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Contents Variation Analysis ofγ-Amino Butyric Acid in Semen sojae praeparatum fermentation Using Online Pre-Column Derivatization-HPLC

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Wuhan University Journal of Natural Sciences 2021年第1期26卷 55-61页

作者： WENG Meizhi HE Jing REN Jiaxiu LIANG Yonghong CHEN Qingfeng SU Mingsheng WANG Liyuan XIE Xiaomei College of Traditional Chinese Medicine Jiangxi University of Traditional Chinese MedicineNanchang 330004JiangxiChina Institute of Translational Medicine Nanchang UniversityNanchang 330031JiangxiChina Key Laboratory of Modem Preparation ofTCM Ministry of EducationJiangxi University of Traditional Chinese MedicineNanchang 330004JiangxiChina

This paper reported the contents variation analysis ofγ-amino butyric acid(GABA)in Semen sojae praeparatum(SSP)which is a famous traditional Chinese *** performance liquid chromatography(HPLC)was used and GABA was derivatized by online pre-column derivatization with o-phthalaldehyde(OPA).To validate this method,the precision,stability,repeatability and recovery were *** the concentration range from 0.0125 to 0.400 mg/m L,the calibration curve for GABA was linear and the regression equation was obtained with correlation coefficient(R2)of *** high levels of GABA exist in SSP and the content changes of GABA at different time points during the fermenting process were *** the"yellow cladding"stage,GABA level was very low or even undetectable;the"secondary fermentation"stage witnessed a rapid increase of GABA content to 1.39-5.52 mg/g,which remained stable after 18 days of"secondary fermentation".This study demonstrated that GABA was generated at the"secondary fermentation"stage,revealing the significance and rationality of the"secondary fermentation"stage in the fermenting process of *** the other hand,it suggested the downside of taking soy isoflavones as the only measurement in existing quality assessment and optimization approach for the fermenting process of SSP.

关键词： Semen sojae praeparatum γ-amino butyric acid(GABA) "secondary fermentation"process online pre-column derivatization-high performance liquid chromatography

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