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Colonization of endophyte Acremonium sp. D212 in Panax notoginseng and rice mediated by auxin and jasmonic acid

Journal of Integrative Plant Biology 2020年第9期62卷 1433-1451页

作者： Li Han Xuan Zhou Yiting Zhao Shusheng Zhu Lixia Wu Yunlu He Xiangrui Ping Xinqi Lu Wuying Huang Jie Qian Lina Zhang Xi Jiang Dan Zhu Chongyu Luo Saijie Li Qian Dong Qijing Fu Kaiyuan Deng Xin Wang Lei Wang Sheng Peng Jinsong Wu Weimin Li JiǐíFriml Youyong Zhu Xiahong He Yunlong Du College of Plant Protection Yunnan Agricultural UniversityKunming 650201China State Key Laboratory for Conservation and Utilization of Bio-Resources in yunnan Yunnan Agricultural UniversityKunming 650201China Key Laboratory of Agro-Biodiversity and Pest Management of Education Ministry of China Yunnan Agricultural UniversityKunming 650201China Industrial Crop research Institute Shanxi Academy of Agricultural SciencesFenyang 032200China Department of Steering Committee Yunnan Agricultural UniversityKunming 650201China yunnan Key Laboratory for Wild Plant Resources Kunming Institute of BotanyChinese Academy of SciencesKunming 650201China Mengzi Seed Management Station Mengzi 661199China Institute of Science and Technology Austria(IST Austria) KlosterneuburgAustria College of Horticulture and Landscape Southwest Forestry UniversityKunming 650224China

Endophytic fungi can be beneficial to plant growth. However, the molecular mechanisms under-lying colonization of Acremonium spp. remain unclear. In this study, a novel endophytic Acremonium strain was isolated from the buds of Panax notoginseng and named Acremonium sp. D212. The Acremonium sp. D212 could colonize the roots of P. notoginseng, enhance the resistance of P. notoginseng to root rot disease, and promote root growth and saponin bio-synthesis in P. notoginseng. Acremonium sp. D212 could secrete indole-3-acetic acid (IAA) and jasmonic acid (JA), and inoculation with the fungus increased the endogenous levels of IAA and JA in P. noto-ginseng. Colonization of the Acremonium sp. D212 in the roots of the rice line Nipponbare was dependent on the concentration of methyl jasmonate (MeJA) (2–15μmol/L) and 1-naphthalenacetic acid (NAA) (10–20μmol/L). Moreover, the roots of the JA signaling-defective coi1-18 mutant were colonized by Acremonium sp. D212 to a lesser degree than those of the wild-type Nipponbare and miR393b-overexpressing lines, and the colonization was res-cued by MeJA but not by NAA. It suggests that the cross-talk between JA signaling and the auxin biosynthetic pathway plays a crucial role in the colonization of Acremonium sp. D212 in host plants.

关键词： roots endogenous expressing

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Haploids can be induced in knockout mutants of OsPLA1,but not OsDMP3 or OsDMP6,in rice

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The Crop Journal 2024年第1期12卷 213-221页

作者： Zongkai Liu Yu Zhong Xiaolong Qi Tai An Shuwei Guo Dong Wang Yuwen Wang Bin Feng Zuofeng Zhu Shaojiang Chen Chenxu Liu National Maize Improvement Center Engineering Research Center of Ministry of EducationChina Agricultural UniversityBeijing 100193China National Center for Evaluation of Agricultural Wild Plants(Rice) Department of Plant Genetics and BreedingChina Agricultural UniversityBeijing 100193China

Doubled haploid(DH)technology is an important tool in crop breeding because it can significantly accelerate the breeding ***1/MATL/NLD and ZmDMP are two key genes controlling haploid induction(HI)in maize,exhibiting a synergistic ***,it is unknown whether knock out of ZmDMP orthologs can stimulate HI in *** this study,a ZmPLA1 ortholog(OsPLA1)and two ZmDMP orthologs(OsDMP3 and OsDMP6)were identified in *** three genes encode plasma membrane-localized proteins and were highly expressed in mature *** of OsPLA1 in both Minghui 63 and Nipponbare resulted in reduced seed setting rate(SSR)and caused *** osdmp3,osdmp6 and the double mutant failed to trigger HI independently,nor increased the haploid induction rate(HIR)when combined with *** pollinations operations of QX654A with the ospla1 mutant significantly improve SSR,while reducing ***-seq profiling of mature ospla1 mutant anthers indicated that a large number of differentially expressed genes(DEGs)were enriched in redox homeostasis and lipid metabolic GO terms,plant hormone signal transduction,and MAPK signaling *** findings provide important insights towards construction of an efficient DH breeding technology and study of the molecular mechanism of HI in rice.

关键词： Doubled haploid breeding Haploid induction Mutation anlaysis Rice

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Growth and metal uptake of energy sugarcane (Saccharum spp.) in different metal mine tailings with soil amendments

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Journal of Environmental Sciences 2014年第5期26卷 1080-1089页

作者： Xin Zhang Yongguan Zhu Yuebin Zhang Yunxia Liu Shaochun Liu Jiawen Guo Rudan Li Songlin Wu Baodong Chen State key Laboratory of Urban and Regional Ecology Research Center for Eco-Environmental SciencesChinese Academy of Sciences Key Lab of Urban Environment and Health Institute of Urban EnvironmentChinese Academy of Sciences Sugarcane research Institute Yunnan Academy of Agricultural Sciences

A pot experiment was conducted to investigate the feasibility of growing energy sugarcane （Sac- charum spp.） in three different metal mine railings （Cu, Sn and Pb/Zn tailings） amended with uncontaminated soil at different mixing ratios. The results indicated that sugarcane was highly tolerant to tailing environments. Amendments of 20% soil to Sn tailings and 30% soil to Cu tailings could increase the biomass of cane-stem for use as the raw material for bioethanol production. Heavy metals were mostly retained in roots, which indicated that sugarcane was useful for the stabilization of the tailings. Bagasse and juice, as the most valuable parts to produce bioethanol, only accounted for 0.6%- 3% and 0.6%-7% of the total metal content. Our study supported the potential use of sugarcane for tailing phytostabilization and bioenergy production.

关键词： bioenergy crop sugarcane metal mine tailings phytoremediation

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Effects of different energy levels in low-protein diet on liver lipid metabolism in the late-phase laying hens through the gut-liver axis

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Journal of Animal Science and Biotechnology 2024年第5期15卷 2122-2136页

作者： Hong Hu Ying Huang Anjian Li Qianhui Mi Kunping Wang Liang Chen Zelong Zhao Qiang Zhang Xi Bai Hongbin Pan yunnan Provincial Key Laboratory of Animal Nutrition and Feed Science Faculty of Animal Science and TechnologyYunnan Agricultural UniversityKunming 650201China College of Animal Science Anhui Science and Technology UniversityBengbu 233000China State Key Laboratory of Animal Nutrition Institute of Animal SciencesChinese Academy of Agriculture SciencesBeijing 100193China Shanghai BIOZERON Biotechnology Co. LtdShanghai 201800China WOD Poultry research Institute Beijing 100193China

Background The energy/protein imbalance in a low-protein diet induces lipid metabolism disorders in late-phase laying *** energy levels in the low-protein diet to adjust the energy-to-protein ratio may improve fat deposition,but this also decreases the laying performance of *** study investigated the mechanism by which different energy levels in the low-protein diet influences liver lipid metabolism in late-phase laying hens through the enterohepatic axis to guide feed optimization and nutrition strategies.A total of 288 laying hens were randomly allocated to the normal-energy and normal-protein diet group(positive control:CK)or 1 of 3 groups:lowenergy and low-protein diet(LL),normal-energy and low-protein diet(NL),and high-energy and low-protein diet(HL)*** energy-to-protein ratios of the CK,LL,NL,and HL diets were 0.67,0.74,0.77,and 0.80,*** Compared with the CK group,egg quality deteriorated with increasing energy intake in late-phase laying hens fed low-protein *** fed LL,NL,and HL diets had significantly higher triglyceride,total cholesterol,acetylCo A carboxylase,and fatty acid synthase levels,but significantly lower hepatic lipase levels compared with the CK *** transcriptome sequencing revealed that genes involved in fatty acid beta-oxidation(ACOX1,HADHA,EHHADH,and ACAA1)were downregulated,whereas genes related to fatty acid synthesis(SCD,FASN,and ACACA)were upregulated in LL group compared with the CK *** of the cecal microbiome showed that in hens fed an LL diet,Lactobacillus and Desulfovibrio were enriched,whereas riboflavin metabolism was *** metabolites that were most significantly affected by the LL diet included several vitamins,such as riboflavin(vitamin B2),pantethine(vitamin B5 derivative),pyridoxine(vitamin B6),and 4-pyridoxic *** A lipid metabolism disorder due to deficiencies of vitamin B2 and pantethine originating from the metabolism of the cecal microb

关键词： Cecal microbiome Energy/protein imbalance Late-phase laying hens laying hens Liver lipid metabolism Low-protein diet Multi-omics

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N-P fertilization did not reduce AMF abundance or diversity but alter AMF composition in an alpine grassland infested by a root hemiparasitic plant

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Plant Diversity 2018年第3期40卷 117-126页

作者： Xuezhao Wang Xiaolin Sui Yanyan Liu Lei Xiang Ting Zhang Juanjuan Fu Airong Li Peizhi Yang Department of Grassland Science College of Animal Science and Technology Northwest A&F University Department of Economic Plants and Biotechnology Yunnan Key Laboratory for Wild Plant Resources Kunming Institute of Botany Chinese Academy of Sciences Key Laboratory of Biogeography and Bioresource in Arid Land Xinjiang Institute of Ecology and Geography Chinese Academy of Sciences

Fertilization has been shown to have suppressive effects on arbuscular mycorrhizal fungi(AMF) and root hemiparasites separately in numerous investigations, but its effects on AMF in the presence of root hemiparasites remain untested. In view of the contrasting nutritional effects of AMF and root hemiparasites on host plants, we tested the hypothesis that fertilization may not show strong suppressive effects on AMF when a plant community was infested by abundant hemiparasitic plants. Plants and soil samples were collected from experimental field plots in Bayanbulak Grassland, where N and P fertilizers had been applied for three continuous years for control against a spreading root hemiparasite, Pedicularis kansuensis. Shoot and root biomass of each plant functional group were determined. Root AMF colonization levels, soil spore abundance, and extraradical hyphae length density were measured for three soil depths(0 e10 cm, 10 e20 cm, 20 e30 cm). Partial 18 S r RNA gene sequencing was used to detect AMF diversity and community composition. In addition, we analyzed the relationship between relative abundance of different AMF genera and environmental factors using Spearman's correlation method. In contrast to suppressive effects reported by many previous studies, fertilization showed no significant effects on AMF root colonization or AMF species diversity in the soil. Instead, a marked increase in soil spore abundance and extraradical hyphae length density were observed. However, fertilization altered relative abundance and AMF composition in the soil. Our results support the hypothesis that fertilization does not significantly influence the abundance and diversity of AMF in a plant community infested by P. kansuensis.

关键词： Arbuscular mycorrhizal fungi Fertilization Community structure Alpine grassland ecosystem Pedicularis

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Trimethylamine oxide supplementation differentially regulates fat deposition in liver, longissimus dorsi muscle and adipose tissue of growing-finishing pigs

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Animal Nutrition 2024年第2期17卷 25-35页

作者： Andong Zha Wanquan Li Jing Wang Ping Bai Ming Qi Peng Liao Bie Tan Yulong Yin Laboratory of Animal Nutritional Physiology and Metabolic Process Key Laboratory of Agro-ecological Processes in Subtropical RegionNational Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry ProductionInstitute of Subtropical AgricultureChinese Academy of Sciences University of Chinese Academy of Sciences College of Animal Science and Technology Hunan Agricultural University yunnan Southwest agriculture and Animal Husbandry Group Co. Ltd.

Trimethylamine oxide(TMAO) is a microbiota-derived metabolite, and numerous studies have shown that it could regulate fat metabolism in humans and mice. However, few studies have focused on the effects of TMAO on fat deposition in growing-finishing pigs. This study aimed to investigate the effect of TMAO on fat deposition and intestinal microbiota in growing-finishing pigs. Sixteen growing pigs were randomly divided into 2 groups and fed with a basal diet with 0 or 1 g/kg TMAO for 149 d. The intestinal microbial profiles, fat deposition indexes, and fatty acid profiles were measured. These results showed that TMAO supplementation had a tendency to decrease lean body mass(P < 0.10) and significantly increased backfat thickness(P < 0.05), but it did not affect growth performance. TMAO significantly increased total protein(TP) concentration, and reduced alkaline phosphatase(ALP) concentration in serum(P < 0.05). TMAO increased the a diversity of the ileal microbiota community(P < 0.05), and it did not affect the colonic microbial community. TMAO supplementation significantly increased acetate content in the ileum, and Proteobacteria and Escherichia-Shigella were significantly enriched in the TMAO group(P < 0.05). In addition, TMAO decreased fat content, as well as the ratio of linoleic acid, n-6polyunsaturated fatty acids(PUFA), and PUFA in the liver(P < 0.05). On the contrary, TMAO increased intramuscular fat content of the longissimus dorsi muscle, whereas the C18:2n6c ratio was increased,and the n-6 PUFA:PUFA ratio was decreased(P < 0.05). In vitro, 1 mmol/L TMAO treatment significantly upregulated the expression of FASN and SREBP1 in C2C12 cells(P < 0.05). Nevertheless, TMAO also increased adipocyte area and decreased the CPT-1B expression in subcutaneous fat(P < 0.05). Taken together, TMAO supplementation regulated ileal microbial composition and acetate production, and regulated fat distribution and fatty acid composition in growing-finishing pigs. These results

关键词： Growing-finishing pig Trimethylamine oxide Fat deposition Fatty acid composition Intestinal microbiota

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Design and experiment of an air-suction potato seed metering device

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International Journal of Agricultural and Biological Engineering 2016年第5期9卷 33-42页

作者： LüJinqing Yang Ying Li Zihui Shang Qinqin Li Jicheng Liu Zhongyuan Department of Engineering Northeast Agricultural UniversityHarbin 150030China

An air-suction potato seed metering device was designed to improve the performance of the large-size seed crops planting equipment and provide a solution for problems on the mechanical potato seeder,such as high multiple-seeding index,high missing-seeding index,low qualified index,and limited *** main structure and operation parameters in terms of rotating speed of the device,seeds height and pickup vacuum pressure were determined by theoretical *** orthogonal tests,conventional tuber(the triaxial average diameter is about 38-57 mm)test and mini-tuber(the triaxial average diameter is about 12-36 mm)test,were conducted to analyze the influences of these factors on the quality of *** multiple-seeding index,missing-seeding index and qualified index were chosen for evaluating the working *** results of conventional tuber test showed that,the multiple-seeding index was 1.1%,the missing-seeding index was 0.8%and the qualified index was 98.1%under conditions of the 30 r/min rotating speed,25 cm seeds height and 10 kPa pickup vacuum *** results of mini-tuber test showed that the multiple-seeding index was 0.5%,the missing-seeding index was 0.6%and the qualified index was 98.9%under conditions of 35 r/min rotating speed,17 cm seeds height and 3.5 kPa pickup *** working performances of the device can meet the requirements of precision seeding.

关键词： air-suction seed metering device mini-tuber precision seeding potato pickup vacuum

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Two major er1 alleles confer powdery mildew resistance in three pea cultivars bred in yunnan province, China

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The Crop Journal 2016年第5期4卷 353-359页

作者： Suli Sun Yuhua He Cheng Dai Canxing Duan Zhendong Zhu National Key Facility for Crop Gene Resources and Genetic Improvement Institute of Crop Science Chinese Academy of Agricultural Sciences yunnan Academy of Agricultural Sciences

Powdery mildew, caused by Erysiphe pisi D.C., is an important disease of pea(Pisum sativum L.).The use of cultivars carrying powdery mildew resistance alleles at the er1 locus is the most effective and economical means of controlling this disease. The objectives of this study were to screen Chinese elite pea cultivars for resistance to E. pisi and to identify the responsible gene at the er1 locus. Among the 37 pea cultivars tested, three(Yunwan 8, Yunwan 21, and Yunwan 23) were immune to E. pisi infection in phenotypic evaluations. The full-length cD NA sequences of the er1 candidate gene, PsM LO1, from the three resistant cultivars and control plants were analyzed. Comparison of the cD NA sequences of 10 clones revealed differences among the powdery mildew-resistant cultivars, susceptible controls, and wild-type cultivar Sprinter. The observed resistance in Yunwan 8 plants resulted from a point mutation(C → G) at position 680 of PsM LO1 that introduced a stop codon, leading to premature termination of protein synthesis. The responsible resistance allele was identified as er1–1. Powdery mildew resistance in Yunwan 21 and Yunwan 23 plants was caused by identical insertions or deletions in PsM LO1. Three distinct PsM LO1 transcripts were observed in Yunwan 21 and Yunwan 23 plants. These transcripts were characterized by a129-bp deletion and 155- and 220-bp insertions, respectively. The responsible resistance allele was identified as er1–2. We have characterized two important er1 alleles in three E. pisi-resistant pea cultivars bred in yunnan province, China. These cultivars represent important genetic resources for the breeding of powdery mildew-resistant pea cultivars.

关键词： Erysiphe pisi er1 Pea Powdery mildew yunnan province

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Effects of Colchicine on Callus Induction and Differentiation of Erianthus arundinaceus

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Agricultural Biotechnology 2018年第1期7卷 1-4,8页

作者： Li YAO Peifang ZHAO Jun ZHAO Jiayong LIU Wei QIN Caiwen WU yunnan Key Laboratory of Sugarcane Genetic Improvement/Sugarcane research Institute Yunnan Academy of Agricultural Sciences

Erianthus arundinaceum, a related species of the most important sugar crop Saccharum, and is an essential gene resource for stress and disease resistance in sugarcane breeding. Colchicine is the most common and effective chemical inducer in polyploid breeding at present. To detect the effects of colchicine on callus induction and shoot regeneration, E. arundinaceus ＇ Yun 83-183＇, with the chromosome of 2n = 60, was used as the material for studying the effect of different concentrations of 2,4-D and colchicine, treatment time and culture methods on callus induction and shoot regeneration. The results indicated, when the callus was cultured for 7 d in solid medium with 0.07% colchicine or 0.5 d in liquid medium with 0.05% colchicine, callus had no ability of shoot regeneration, which meant that in liquid culture, colchicine had not only high inhibitory effect on callus, but also lower concentration and shorter treatment time than solid culture; when the concentration of colchicine was 0.03% and callus was cultured for 7 d in solid medium or 0.5 d in liquid mhdium, the resistant callus rate and resistant bud rate were the highest, although the resistant callus rates were all about 88.00% and had no significant difference between solid culture and liquid culture, the resistance bud rate was only 6.00% in liquid culture, 16.22% lower than in solid culture, which meant that solid culture was more effective than liquid culture at the same colchicine concentration. For E. arundinaceus ＇ Yun 83-183＇ , the best concentration of 2,4-D was 2 mg/L, the best concentration of colchicine was 0.03%, and the best effect of callus induction and shoot regeneration was achieved within 7 d of solid culture. This study would provide fundamental information for chromosome doubling of E. arundinaceus in the future.

关键词： Erianthus arundinaceum Colchieine Callus

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Karyotype Comparison of F_1 and F_2 Hybrids of Sugarcane and Erianthus fulvus

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Agricultural Biotechnology 2015年第1期4卷 36-41页

作者： Xianhong WANG Fusheng LI Lilian HE Hongbo LOU Qinghui YANG Shunchang HE Sugarcane research Institute Yunnan Agricultural University

Chromosome specimens of sugarcane, Erianthus fu/vus and their progenies were prepared with wall degradation hypotonic method to analyze the karyotypes of parents and Fl, F2 hybrids. The results showed that most chromosomes of experimental materials are median region （m） chromosomes, while only a small amount of chromosomes are submedian （sm） chromosomes. The karyotype of YAU04/102 belongs to 2C type, while other materials belong to 2B type. In addition, the karyotypes of hybrids are asymmetrical and the chromosomes of hybrids show an evolutionary trend compared with parental chromosomes.

关键词： Sugarcane Erianthus fidvus Hybridization Karyotype

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