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Strategic Conservation of Orchard Germplasm Based on Indigenous Knowledge and Genetic Diversity:a Case Study of Sour Orange Populations in china

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Journal of Integrative Plant Biology 2009年第1期51卷 100-106页

作者： Feng Ming Qi-Kun Liu Jin-Lei Shi Wei Wang Bao-Rong Lu Institute of Genetics State Key Laboratory of Genetic Engineering Fudan University Shanghai 200433 China Ministry of Education Key Laboratory for Biodiversity science and Ecological Engineering Institute of Biodiversity Science Fudan University Shanghai 200433 China

To effectively conserve sour orange （Citrus aurantium L.） germplasm on two islands at the estuary of the Yangtze River in china, we estimated genetic variation and relationships of the known parental trees and their proposed descendents （young trees） using the fingerprints of random amplified polymorphic DNA （RAPD）. Results based on RAPD analyses showed considerable genetic diversity in the parental populations （He = 0.202）. The overall populations including the parental and young trees showed slightly higher genetic diversity （He = 0.298） than the parents, with about 10% variation between populations. An unweighted pair group method with arithmetic mean analysis dendrogram based on cluster analysis of the Jaccard similarity among individuals demonstrated a more complicated relationship of the parental and young trees from the two islands, although the young trees showed a clear association with parental trees. This indicates a significant contribution of parental trees in establishing the sour orange populations on the two islands. According to farmers＇ knowledge, conservation of only one or two parental trees would be sufficient because they believed that the whole populations were generated from a single mother tree. However, this study suggests that preserving most parental trees and some selected young trees with distant genetic relationships should be an effective conservation strategy for sour orange germplasm on the two islands.

关键词： Citrus aurantium genetic conservation orchard genetic resources random amplified polymorphic DNA variation relationship.

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Dose-dependent Inhibition of Gynecophoral Canal Protein Gene Expression in Vitro in the Schistosome(Schistosomajaponicum)by RNA Interference

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Acta Biochimica et Biophysica Sinica 2005年第6期37卷 386-390页

作者： Guo-Feng CHENG~1 Jiao-Jiao LIN~1 Yi SHI~2 You-Xin JIN~2 Zhi-Qiang FU~1 Ya-Mei JIN~1 Yuan-Cong ZHOU~2 You-Min CA~1 1 shanghai Institute of Animal Parasitology, Chinese Academy of Agricultural sciences, Key Lahoratory of Animal Parasitology,Ministry of Agriculture, shanghai 200232, china 2 State Key Laboratory, of Molecular Biology Institute of Biochemistry and Cell Biology shanghai Institutes for Biological sciences, Chinese Academy of sciences. shanghai 200031, china shanghai Institute of Animal Parasitology Chinese Academy of Agricultural Sciences Key Laboratory of Animal Parasitology Ministry of Agriculture Shanghai 200232 China.

The gynecophoral canal protein gene SjGCP of Schistosoma japonicum that is necessary forthe pairing between the male and female worms is specifically expressed in the adult male *** is widely distributed in the adult female worm after *** transcription-polymerase chainreaction(RT-PCR)and immunofluorescence were employed to analyze the relationship between the RNAieffect and dsRNA dosage in the *** results revealed that the inhibition of SjGCP expression bysiRNA is ***-PCR analysis showed that the SjGCP transcript level was reduced by 75%when 100 nM dsRNA was applied.

关键词： RNA interference gynecophoral canal protein gene Schistosoma japonicum dosage

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Constitutive expression of human coagulating factor IX in HeLa cells by homologous recombination of the promoter

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science china(Life sciences) 2001年第1期44卷 18-24页

作者：杨晓青王飞王颖卢大儒邱信芳薛京伦 1. State Key Laboratory of Genetics Engineering Institute of Genetics School of Life Sciences Fudan University 200433 Shanghai China

Constitutive expression of hFIX protein in nonhepatocytes wasstudied. The gene targeting vector was constructed and transferred into HeLa cells. With the detection system of PCR, we demonstrated that the endogenous hFIX promoter was replaced with an hCMV promoter when targeted insertion of the constructor was directed by the sequence homology. The expression of hFIX in the modified HeLa cells, 11.2 ng/106 cell/24 h, strongly suggested that hFIX gene could be activated by a powerful promoter in nonhepatocytes. The results would make it possible to examine the feasibility of re-regulate gene expression by promoter replacement.

关键词： human clotting factor IX constitutive expression homologous recombination promoter.

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How to measure the internuclear vector from photoelectron angular distributions

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Chinese Physics B 2010年第2期19卷 194-199页

作者：任向河张敬涛王懿徐至展 J.T.Wang D.S.Guo State Key Laboratory for High-Field Laser Physics Shanghai Institute of Optics and Fine MechanicsChinese Academy of Sciences Department of Physics Southern University and A&M CollegeBaton RougeLouisiana 70813 USA

This paper uses a nonperturbative scattering theory to study photoelectron angular distributions of homonuclear diatomic molecules irradiated by circularly polarized laser fields. This study shows that the nonisotropic feature of photoelectron angular distributions is not due to the polarization of the laser field but the internuclear vector of the molecules. It suggests a method to measure the molecular orientation and the internuclear distance of molecules through the measurement of photoelectron angular distributions.

关键词： photoelectron angular distributions internuclear distance photoionization

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Bilirubin adsorption property of mesoporous silica and amine-grafted mesoporous silica

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Nano-Micro Letters 2009年第1期1卷 14-18页

作者： L.X.Zhang M.Zhu L.M.Guo L.Li J.L.Shi State Key Laboratory of High Performance and Superfine Microstructure Shanghai Institute of Ceramics Chinese Academy of Science

Nephritic, hepatic and immune failures would lead to the overload of endogenous toxic molecules(e.g. bilirubin, cholic acid, uric acid, creatinine etc.) in human bodies. It is fatal in most cases and extracorporeal blood purification(ECBP) is powerful first-aid therapy. Adsorbents are key parts of ECBP apparatus. Mesoporous silicas should be promising candidates for these medical adsorbents, but there is no report about this. Herein, pure and amine-grafted mesoporous silicas have been applied to adsorb bilirubin, cholic acid, uric acid, creatinine and phenobarbital for the first time. These mesoporous materials show high adsorption capacities for bilirubin and uric acid in phosphate buffer solution(PBS). Effects of pore sizes, amine-modification, temperature and ionic strength on their bilirubin adsorption capacities have been studied in detail.

关键词： Mesoporous silica Bilirubin adsorption Amine-grafted

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Remarks on the Operator Norm Localization Property

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Chinese Annals of Mathematics,Series B 2011年第4期32卷 593-604页

作者： Xianjin WANG Department of Applied Mathematics Donghua University Shanghai 201620 China.

The author studies the metric spaces with operator norm localization property. It is proved that the operator norm localization property is coarsely invariant and is preserved under certain infinite union. In the case... 详细信息

关键词： Metric space Asymptotic dimension Operator norm localization Coarse invariance Roe algebra

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Immobilization of HX:[Hmim]X as Halogenating Agent, Recyclable Catalyst and Medium for Conversion of Alcohols to Alkyl Halides

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Chinese Journal of Chemistry 2004年第7期22卷 619-621页

作者：吴海虹孙靖杨帆汤杰何鸣元 Department of Chemistry East China Normal University Shanghai 200062 China

HX is immobilized by reaction of halogen acid with methylimidazole, and the formed ionic liquid [Hmim]X was used as halogenating agent, catalyst as well as medium for conversion of alcohols to alkyl halides. Excellent yields were obtained. The halides produced could be easily separated from the reaction mixture via simple decantation, and the ionic liquid [Hmim]X could be regenerated conveniently by adding equivalent of halogen acids followed by removal of water.

关键词： halogenation ionic liquid alcohol alkyl halide

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Nonlinear H_∞ control of structured uncertain stochastic neural networks with discrete and distributed time varying delays

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Chinese Physics B 2008年第4期17卷 1506-1512页

作者：陈狄岚张卫东 Department of Automation Shanghai Jiaotong University Division of Basic Courses Shanghai Maritime University

This paper is concerned with the problem of robust H∞ control for structured uncertain stochastic neural networks with both discrete and distributed time varying delays. A sufficient condition is presented for the existence of H∞ control based on the Lyapunov stability theory. The stability criterion is described in terms of linear matrix inequalities （LMIs）, which can be easily checked in practice. An example is provided to demonstrate the effectiveness of the proposed result.

关键词： delayed neural networks (DNNs) stochastic systems Lyapunov functional linear matrix inequality

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Modeling and simulation for a new virtual-clock-based collision resolution algorithm

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High Technology Letters 2006年第2期12卷 124-128页

作者：尹汝泼 Cai yunze He xing Zhang weidong Xu xiaoming Department of Automation Shanghai Jiaotong University Shanghai 200030 P.R. China

Virtual time Ethemet is a multiple access protocol proposed to provide FCFS transmission service over the predominant Ethemet bus. It incorporates a novel message-rescheduling algorithm based on the virtual clock mechanism. By manipulating virtual clocks back up over a common virtual time axis and performing timely collision resolution, the algorithm guarantees the system＇s queuing strictness. The protocol is particularly modeled as a finite state machine and implemented using OPNET tools. Simulation studies prove its correctness and effectiveness.

关键词： collision resolution virtual clock Ethernet OPNET

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Cloning,expression and function of phosphate transporter encoded gene in Oryza sativa L.

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science china(Life sciences) 2006年第5期49卷 409-413页

作者： MING Feng LU Qun WANG Wei ZHANG Shanshan GUO Bin SHEN Daleng Institute of Genetics State Key Laboratory of Genetic EngineeringSchool of Life ScienceFudan UniversityShanghai 200433China Ministry of Education Key Laboratory for Biodiversity science and Ecological Engineering Institute of Biodiversity ScienceSchool of Life ScienceFudan UniversityShanghai 200433China

OsPT6:1,a phosphate transporter encoding gene from the leaf samples of Oryza sativa, was identified through PCR with specifically designed *** phylogenetic analysis and the conserved amino acid residue site detection suggested OsPT6:1 a possible high-affinity phosphate transporter encoding *** situ hybridization and RT-PCR demonstrated the expression of OsPT6:1 in both roots and *** peak expression signal was observed in mesophyll cells under low phosphorus(P)induction.A homologous recombination study indicated that OsPT6:1 can enhance the Pi uptake efficiency of Pichia *** the meantime,the introduction of OsPT6:1 was able to complement the Pi uptake function of yeast cells with high-affinity phosphate transporters de- *** results substantiated our contention that OsPT6:1 encoded a high-affinity phosphate transporter of Oryza sativa.

关键词： high-affinity phosphate transporter Oryza sativa in situ hybridization function complementation

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