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Proinflammatory effects and molecular mechanisms of interleukin-17 in intestinal epithelial cell line HT-29

World Journal of Gastroenterology 2014年第47期20卷 17924-17931页

作者： Yi-Lin Wang Meng Fang Xiao-Ming Wang Wei-Yan Liu Yun-Jiang Zheng Xu-Bo Wu Ran Tao Center for Organ Transplantation and Department of Surgery Ruijin HospitalShanghai Jiaotong University School of Medicine Department of General Surgery Minhang HospitalFudan University Department of Anesthesia The 88th Hospital of PLA Department of Hepatobiliary Surgery Yijishan HospitalWannan Medical College Department of Emergency Xin Hua HospitalShanghai Jiaotong University School of Medicine

AIM:To evaluate the proinflammatory effects and molecular mechanisms of interleukin(IL)-17 in intestinal epithelial cell line ***:HT-29 cells were cultured with IL-17,tumor necrosis factor(TNF)-α,or the combination of both IL-17 and TNF-α.Real-time PCR and Western blot were used to measure the gene expression levels of neutrophil chemokines CXCL1,CXCL2,CXCL5,CXCL6,IL-8and TH-17 cell chemokine CCL20,the phosphorylation levels of p38 and TNF-α,and the expression level of IL-8,after using the p38 inhibitor in HT-29 *** stable Act1 knockdown HT-29 cell line was established to further test the phosphorylation changes of p38,after using IL-17 and TNF-α.RESULTS:After HT-29 cells were cultured with IL-17and TNF-α,the expression levels of neutrophil chemokines(CXCL1,CXCL2,CXCL5,CXCL6,IL-8)and Th17chemokine(CCL20)significantly improved(24.96±2.53,28.47±2.87,38.08±2.72,33.47±2.41,31.7±2.38,44.37±2.73,respectively),and the differences were all statistically significant(P<0.01).Western blot results showed that IL-17 obviously enhanced the phosphorylation level of p38,which was induced by TNF-α.Compared with the control group,the expression level of IL-8 significantly declined(9.47±1.36 vs 3.06±0.67,P<0.01)when TH-29 cells were cultured with IL-17and TNF-α.p38 inhibition assay showed that the p38pathway played an essential role in the inflammatory response induced by IL-17.p38 phosphorylation levels could not be changed after using IL-17 and TNF-αin the stable Act1 knockdown HT-29 cell ***:IL-17 significantly promoted the gene expression levels of TNF-α-induced neutrophil chemokines and Th17 cell *** is obvious that IL-17and TNF-αhave synergistic effects on p38.

关键词： IL-17 HT-29 TNF-α Inflammatory bowel disease

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Hepatocyte differentiation of mesenchymal stem cells

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Hepatobiliary & Pancreatic Diseases International 2012年第4期11卷 360-371页

作者： Xu-Bo Wu Department of General Surgery,Central Hospital of Minhang District,Ruijin Medical Group,shanghai 201199,China Ran Tao Center for Organ Transplantation,Department of General Surgery,Ruijin Hospital,shanghai Jiaotong University School of Medicine,shanghai 200025,China [a] Department of General Surgery Central Hospital of Minhang District Ruijin Medical Group Shanghai 201199 China [b] Center for Organ Transplantation Department of General Surgery Ruijin Hospital Shanghai Jiaotong University School of Medicine Shanghai 200025 China

BACKGROUND:Liver cell transplantation and bioartificial liver may provide metabolic support of liver function temporary and are prospective treatments for patients with liver *** stem cells(MSCs) are expected to be an ideal cell source for transplantation or liver tissue engineering,however the hepatic differentiation of MSCs is still insufficient for clinical *** SOURCES:A PubMed search on 'mesenchymal stem cells','liver cell' and 'hepatocyte differentiation' was performed on the topic,and the relevant articles published in the past ten years were ***:Hepatocyte-like cells differentiated from MSCs are a promising cell source for liver regeneration or tissue *** it is still a matter of debate as to whether MSC-derived hepatocytes may efficiently repopulate a host liver to provide adequate functional substitution,the majority of animal studies support that MSCs can become key players in liver-directed regenerative *** the clinical application of human stem cells in the treatment of liver diseases is still in its ***:Future studies are required to improve the efficacy and consistency of hepatic differentiation from *** is necessary to better understand the mechanism to achieve transdifferentiation with high *** clinical trials are warranted to prove their efficacy in the management of patients with liver failure.

关键词： hepatocyte differentiation mesenchymal stem cells

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