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Astrocyte-neuron communication mediated by the Notch signaling pathway:focusing on glutamate transport and synaptic plasticity

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Neural Regeneration Research 2023年第10期18卷 2285-2290页

作者： Ke-Xin Li Meng Lu Meng-Xu Cui Xiao-Ming Wang Yang Zheng Department of Radiology Shengjing Hospital of China Medical UniversityShenyangLiaoning ProvinceChina

Maintaining glutamate homeostasis after hypoxic ischemia is important for synaptic function and neural cell activity,and regulation of glutamate transport between astrocyte and neuron is one of the important modalities for reducing glutamate ***,further research is needed to investigate the dynamic changes in and molecular mechanisms of glutamate transport and the effects of glutamate transport on *** aim of this study was to investigate the regulatory mechanisms underlying Notch pathway mediation of glutamate transport and synaptic *** this study,Yorkshire neonatal pigs(male,age 3 days,weight 1.0–1.5 kg,n=48)were randomly divided into control(sham surgery group)and five hypoxic ischemia subgroups,according to different recovery time,which were then further subdivided into subgroups treated with dimethyl sulfoxide or a Notch pathway inhibitor(N-[N-(3,5-difluorophenacetyl-l-alanyl)]-S-phenylglycine t-butyl ester).Once the model was established,immunohistochemistry,immunofluorescence staining,and western blot analyses of Notch pathway-related proteins,synaptophysin,and glutamate transporter were ***,synapse microstructure was observed by transmission electron *** the early stage(6–12 hours after hypoxic ischemia)of hypoxic ischemic injury,expression of glutamate transporter excitatory amino acid transporter-2 and synaptophysin was downregulated,the number of synaptic vesicles was reduced,and synaptic swelling was observed;at 12–24 hours after hypoxic ischemia,the Notch pathway was activated,excitatory amino acid transporter-2 and synaptophysin expression was increased,and the number of synaptic vesicles was slightly *** amino acid transporter-2 and synaptophysin expression decreased after treatment with the Notch pathway *** suggests that glutamate transport in astrocytes-neurons after hypoxic ischemic injury is regulated by the Notch pathway and affects vesicle release and synapt

关键词： astrocyte astrocyte-neuron communication glutamate glutamate transporter hypoxic-ischemic injury magnetic resonance spectroscopy neonate Notch signaling pathway plasticity synapse

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Emodin attenuates inflammation and demyelination in experimental autoimmune encephalomyelitis

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Neural Regeneration Research 2023年第7期18卷 1535-1541页

作者： Yue-Ran Cui Zhong-Qi Bu Hai-Yang Yu Li-Li Yan Juan Feng Department of Neurology Shengjing Hospital of China Medical UniversityShenyangLiaoning ProvinceChina

Emodin,a substance extracted from herbs such as rhubarb,has a protective effect on the central nervous ***,the potential therapeutic effect of emodin in the context of multiple sclerosis remains *** this study,a rat model of experimental autoimmune encephalomyelitis was established by immune induction to simulate multiple sclerosis,and the rats were intraperitoneally injected with emodin(20 mg/kg/d)from the day of immune induction until they were *** this model,the nucleotide-binding domain-like receptor family pyrin domain containing 3(NLRP3)inflammasome and the microglia exacerbated neuroinflammation,playing an important role in the development of multiple *** addition,silent information regulator of transcription 1(SIRT1)/peroxisome proliferator-activated receptor-alpha coactivator(PGC-1α)was found to inhibit activation of the NLRP3 inflammasome,and SIRT1 activation reduced disease severity in experimental autoimmune ***,treatment with emodin decreased body weight loss and neurobehavioral deficits,alleviated inflammatory cell infiltration and demyelination,reduced the expression of inflammatory cytokines,inhibited microglial aggregation and activation,decreased the levels of NLRP3 signaling pathway molecules,and increased the expression of SIRT1 and PGC-1α.These findings suggest that emodin improves the symptoms of experimental autoimmune encephalomyelitis,possibly through regulating the SIRT1/PGC-1α/NLRP3 signaling pathway and inhibiting microglial *** findings provide experimental evidence for treatment of multiple sclerosis with emodin,enlarging the scope of clinical application for emodin.

关键词： demyelination emodin experimental autoimmune encephalomyelitis microglia multiple sclerosis neuroinflammation NLRP3 inflammasome PGC-1α pyroptosis SIRT1

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Live birth following an innovative artificial oocyte activation protocol using double calcium stimulators

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Chinese Medical Journal 2023年第17期136卷 2101-2103页

作者： Yingzhuo Gao Dalei Yang Yuanyuan Fang Xiuxia Wang Da Li Center of Reproductive Medicine Shengjing Hospital of China Medical UniversityShenyangLiaoning 110004China

Intracytoplasmic sperm injection(ICSI)is an effective method for human-assisted reproductive technology,especially for male-factor ***,the average fertilization rate of ICSI is 70%,and total fertilization failure occu... 详细信息

关键词： stimulator injection activation

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Magnetic Resonance Three-dimensional Cube Technique in the Measurement of Piglet Femoral Anteversion

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Chinese Medical Journal 2016年第13期129卷 1584-1591页

作者： Dong-Mei Sun Shi-Nong Pan En-Bo Wang Li-Qiang Zheng Wen-Li Guo Xi-Hu Fu Department of Radiology Shengjing Hospital of China Medical University Shenyang Liaoning 110004 China Department of Pediatric Orthopedics Shengjing Hospital of China Medical University Shenyang Liaoning 110004 China Department of Clinical Epidemiology Shengjing Hospital of China Medical University Shenyang Liaoning 110004 China

Background： The accurate measurement of the femoral anteversion （FA） angle is always a topic of much debate in the orthopedic surgery and radiology research. We aimed to explore a new FA measurement method to acquire accurate results without radiation damage using piglet model. Methods： A total of thirty piglets were assigned to two groups based on the age. Bilateral femora were imaged with 3.0-T magnetic resonance （MR） and 64-slice computed tomography （CT） examinations on all piglets. FA was measured on MR-three-dimensional （3D） postprocessing software with a four-step method： initial validation of the femoral condylar axis, validation of the condylar plane, validation of the femoral neck axis, and line-plane angle measurement of FA. After MR and CT examinations, all piglets were sacrificed and their degree of FA was measured using their excised, dried femora. MR, CT, and dried-femur measurement results were analyzed statistically; M R and CT measurements were compared for accuracy against each other and against the gold standard dried femur measurement. Results： In both groups, the mean FA value measured by MR was lower than that measured by CT. A statistically significant difference was observed between CT- and dried-femur measurements but not between MR- and dried-femur measurements. A higher correlation （0.783 vs. 0.408） and a higher consistency （0.863 vs. 0.578） with dried-femur measurement results were seen for MR measurements than CT measurements in the 1 -week age group. However, in the 8-week age group, similar correlations （0.707 vs. 0.669） and consistencies （0.864 vs. 0.82 l ） were observed. Conclusions： Noninvasive MR-3D-Cube reconstruction was able to accurately measure FA in piglets. Particularly in the 1-week age group with a larger proportion of cartilaginous structures, the correlation and consistency between MR- and dried-femur measurement results were higher than those between CT- and dried-femur measurements, suggesting that MR may be a new usef

关键词： Femoral Anteversion Hip Magnetic Resonance Imaging Piglet Three-dimensional Fast Spin Echo Cube

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Mesenteric heterotopic pancreas in a pediatric patient: A case report and review of literature

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World Journal of Clinical Cases 2018年第14期6卷 847-853页

作者： Xiao-Bing Tang Min-Yi Liao Wei-Lin Wang Yu-Zuo Bai Department of Pediatric Surgery Shengjing HospitalChina Medical University

Heterotopic pancreas （HP） is a congenital anomaly defined as pancreatic tissue that has no contact with the orthotopic pancreas and its own duct system and vascular supply. The most common locations of HP are the upper gastrointestinal tract, specifically, the stomach, duodenum, and proximal jejunum. In-volvement of the mesentery is rare. Here, we describe a rare case of mesenteric heterotopic pancreas （MHP） in a 12-year-old girl who presented with acute abdomen. The patient underwent emergency laparotomy, and the mass and adjacent small bowel were resected. Results of the postoperative histopathologic examination confirmed the diagnosis of MHP. Observation of the patient for 12 mo postoperatively showed no evidence of recurrence. Preoperative diagnosis of HP is diffcult, even in a symptomatic patient. Increased awarenessand understanding of the image characteristics of MHP will aid in correct preoperative diagnosis and appropriate patient management.

关键词： Heterotopic pancreas Mesenteric Acute abdomen Computed tomography Magnetic resonance imaging Case report

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PRPF6 promotes metastasis and paclitaxel resistance of ovarian cancer via SNHG16/CEBPB/GATA3 axis

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Oncology Research 2021年第4期29卷 275-289页

作者： HAN WANG YINGYING ZHOU SIYANG ZHANG YA QI MIN WANG Department of Gynecology and Obstetrics Shengjing Hospital of China Medical UniversityShenyangChina

Metastasis and paclitaxel(PTX)resistance are the main reason for the poor prognosis of ovarian cancer(OC).Evidence showed that RNA-binding proteins(RBPs)and long noncoding RNAs(lncRNAs)can modulate post-transcriptional *** aim of this study was to determine the relationship among RBP,lncRNA and OC and to further guide clinical *** revealed that pre-mRNA processing factor 6(PRPF6)was upregulated in OC chemoresistant tissues and was closely related to advanced(Federation of International of Gynecologists and Obstetricians)FIGO stages and ***6 promoted progression,and PTX resistance in vitro and in *** the transcripts of small nucleolar RNA host gene SNHG16-L/S were differentially expressed in OC cells and tissues as detected through real-time PCR(RT-PCR).SNHG16-L/S had opposite effects on progression and PTX resistance in ***,SNHG16-L inhibited GATA-binding protein 3(GATA3)transcription by binding to CCAAT/enhancer-binding protein B(CEBPB).Moreover,PRPF6 induced the alternative splicing of SNHG16,causing downregulation of SNHG16-L and,leading to the upregulation of GATA3 expression to further promote metastasis and PTX-resistance in ***,these data unveiled that PRPF6 promotes metastasis and PTX resistance of OC through SNHG16-L/CEBPB/GATA3 axis,which provides a new direction for OC treatment.

关键词： Ovarian cancer PRPF6 SNHG16 Paclitaxel resistance GATA3

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Changes in energy metabolism in the quadriceps femoris after a single bout of acute exhaustive swimming in rats： a 31p-magnetic resonance spectroscopy study

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Chinese Medical Journal 2014年第5期127卷 937-944页

作者： Sun Yingwei Pan Shinong Chen Zhian Zhao Heng Ma Ying Zheng Liqiang Li Qi Deng Chunbo Fu Xihu Lu Zaiming Guo Qiyong Department of Radiology Shengjing Hospital of China Medical UniversityShenyang Liaoning 110004 China

Background Little is known about the value of 31P-magnetic resonance spectroscopy （31P-MRS） in in vivo assessment of exhaustive exercise-induced injury in skeletal *** aimed to evaluate the value of a 31P-MRS study using the quadriceps femoris after a single bout of acute exhaustive swimming in rats,and the correlation between 31P-MRS and histological *** Sixty male Sprague-Dawley rats were randomly assigned to control,half-exhaustive,and exhaustive exercise groups.31P-MRS of the quadriceps femoris of the right lower limb was performed immediately after swimming exercise to detect Pi,PCr,and β-*** Pi/PCr,Pi/β-ATP,PCr/β-ATP,and PCr/（PCr＋Pi） were calculated and pH *** under the receiver operating characteristic curve （AUCs） were calculated to evaluate the diagnostic potential of 31P-MRS in identifying and distinguishing the three *** staining,electron microscopy and desmin immunostaining after imaging of the muscle were used as a reference *** correlation between 31P-MRS and the mean absorbance （A value） of desmin staining were analyzed with the Pearson correlation *** Pi,PCr,Pi/PCr,and PCr/（PCr＋Pi） showed statistically significant intergroup differences （P＜0.05）.AUCs of Pi,PCr,Pi/PCr,and PCr/（PCr＋Pi） were 0.905,0.848,0.930,and 0.930 for the control and half-exhaustive groups,while sensitivity and specificity were 90％/85％,95％/55％,95％/80％,and 90％/85％,*** AUCs of Pi,PCr,Pi/PCr and PCr/（PCr＋Pi） were 0.995,0.980,1.000,and 1.000 for the control and exhaustive groups,while sensitivity and specificity were 95％/90％,100％/90％,100％/95％,and 100％/95％,*** AUCs of Pi,PCr,Pi/PCr,and PCr/（PCr＋Pi） were 0.735,0.865,0.903,and 0.903 for the half-exhaustive and exhaustive groups,while sensitivity and specificity were 80％/60％,90％/75％,95％/65％,and 95％/70％,*** the half-exhaustive group,some muscle fibers exhibited edema in HE staining,and the unclear Z-discs and the mitochondria with vacuolar degener

关键词： magnetic resonance spectroscopy exhaustive exercise energy metabolism receiver operating characteristic curve

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Identification and characterization of a novel group of natural antisense transcripts from RNA1.2 gene locus of human cytomegalovirus

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Chinese Medical Journal 2019年第13期132卷 1591-1598页

作者： Bing-Yang Liu Yan-Ping Ma Ying Qi Yu-Jing Huang Zhong-Yang Liu Meng-Qing Lu Qiang Ruan Department of Endocrinology Affiliated Shengjing HospitalChina Medical UniversityShenyangLiaoning 110004China Virus Laboratory Affiliated Shengjing HospitalChina Medical UniversityShenyangLiaoning 110004China

Background:Natural anti-sense transcripts(NATs),which are transcribed from the complementary DNA strand of annotated genes,exert regulatory function of gene *** studies recognized anti-sense transcription widespread throughout human cytomegalovirus(HCMV)genome,whereas the anti-sense transcription of RNA1.2 gene locus has never been *** this study,the transcription of the RNA1.2 anti-sense strand was investigated in clinically isolated HCMV ***:Strand-specific high-through RNA-sequencing(RNA-seq)was performed to find possible anti-sense transcripts(ASTs).For analyzing and visualization of RNA-seq data sets,Integrative Genomics Viewer software was *** confirm these possibilities,Northern blotting and rapid amplification of cDNA ends(RACE)were ***:Transcription of the opposite strand of RNA1.2 gene locus was detected by RNA-sequencing using RNAs extracted from human embryonic lung fibroblasts infected with HCMV clinical isolate *** least three HCMV NATs,named RNA1.2 AST 1,RNA1.2 AST2,and RNA 1.2 AST3,were characterized by Northern blotting and RACE *** RNA1.2 ASTs orientated from the complementary strand of RNA1.2 locus during the late phase of HCMV *** 5'-and 3'-termini of these transcripts were located within the opposite sequence of the predicted RNA1.2 ***:A cluster of novel NATs was transcribed from the opposite sequence of the HCMV RNA 1.2 gene region.

关键词： Human cytomegalovirus RNA1.2 Natural anti-sense transcript

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Human cytomegalovirus miR-US5-1 inhibits viral replication by targeting Geminin mRNA

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Virologica Sinica 2017年第5期32卷 431-439页

作者： Shujuan Jiang Yujing Huang Ying Qi Rong He Zhongyang Liu Yanping Ma Xin Guo Yaozhong Shao Zhengrong Sun Qiang Ruan Virus Laboratory the Affiliated Shengjing Hospital China Medical University Shenyang 110122 China Clinical Genetics the Affiliated Shengjing Hospital China Medical University Shenyang 110122 China

Viruses commonly create favorable cellular conditions for their survival through multiple mechanisms. Micro RNAs(mi RNAs), which function as post-transcriptional regulators, are utilized by human cytomegalovirus(HCMV) in its infection and pathogenesis. In the present study, the DNA replication inhibitor Geminin(GMNN) was identified to be a direct target of hcmv-mi R-US5-1. Overexpression of hcmv-mi R-US5-1 could block the accumulation of GMNN during HCMV infection, and the decrease of GMNN expression caused by hcmv-mi R-US5-1 or GMNN specific si RNA reduced HCMV DNA copies in U373 cells. Meanwhile, ectopic expression of hcmv-mi R-US5-1 and consequent lower expression of GMNN influenced host cell cycle and proliferation. These results imply that hcmv-mi R-US5-1 may affect viral replication and host cellular environment by regulating expression kinetics of GMNN during HCMV infection.

关键词： Human cytomegalovirus(HCMV) hcmv-mi R-US5-1 Geminin(GMNN) DNA replication cell cycle

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Exendin-4 and linagliptin attenuate neuroinflammation in a mouse model of Parkinson's disease

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Neural Regeneration Research 2023年第8期18卷 1818-1826页

作者： Hai-Yang Yu Tong Sun Zhen Wang Hong Li Duo Xu Jing An Lu-Lu Wen Jia-Yi Li Wen Li Juan Feng Department of Neurology Shengjing Hospital of China Medical UniversityShenyangLiaoning ProvinceChina Department of Pediatrics Shengjing Hospital of China Medical UniversityShenyangLiaoning ProvinceChina Laboratory of Research in Parkinson’s Disease and Related Disorders Health Sciences InstituteChina Medical UniversityShenyangLiaoning ProvinceChina Neural Plasticity and Repair Unit Department of Experimental Medical ScienceLund UniversityLundSweden

Use of glucagon-like peptide-1 receptor agonist or dipeptidyl peptidase 4 inhibitor has been shown to lower the incidence of Parkinson's disease in patients with diabetes ***,using these two treatments may help treat Parkinson's *** further investigate the mechanisms of action of these two compounds,we established a model of Parkinson's disease by treating mice with 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine and then subcutaneously injected them with the glucagon-like peptide-1 receptor agonist exendin-4 or the dipeptidyl peptidase 4 inhibitor *** found that both exendin-4 and linagliptin reversed motor dysfunction,glial activation,and dopaminergic neuronal death in this *** addition,both exendin-4 and linagliptin induced microglial polarization to the anti-inflammatory M2 phenotype and reduced pro-inflammatory cytokine ***,in vitro experiments showed that treatment with exendin-4 and linagliptin inhibited activation of the nucleotide-binding oligomerization domain-and leucine-rich-repeat-and pyrin-domaincontaining 3/caspase-1/interleukin-1βpathway and subsequent pyroptosis by decreasing the production of reactive oxygen *** findings suggest that exendin-4 and linagliptin exert neuroprotective effects by attenuating neuroinflammation through regulation of microglial polarization and the nucleotidebinding oligomerization domain-and leucine-rich-repeat-and pyrin-domain-containing 3/caspase-1/interleukin-1βpathway in a mouse model of Parkinson's disease induced by 1-methyl-4-phenyl-1,2,3,***,these two drugs may serve as novel anti-inflammatory treatments for Parkinson's disease.

关键词： diabetes mellitus dipeptidyl peptidase 4 inhibitor exendin-4 glucagon-like peptide-1 receptor agonist 1-methyl-4-phenyl-1 2 3 6-tetrahydropyridine linagliptin microglia neuroinflammation NLRP3 inflammasome Parkinson's disease pyroptosis

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