检索结果-南通市图书馆

Microfluidic thermotaxic selection of highlymotile sperm and in vitro fertilization

Bio-Design and Manufacturing 2024年第5期7卷 687-700页

作者： Sihan Chen Jiemin Chen Zihan Qin Jibo Wang Yuwen Wang Rong Liu Wen Zhao Ming Zhang Yuanzhen Zhang Mengcheng Luo Pu Chen Tissue Engineering and Organ Manufacturing(TEOM)Lab Department of Biomedical EngineeringTaiKang Medical School(School of Basic Medical Sciences)Wuhan UniversityWuhan 430071China hubei Provincial key Laboratory of Developmentally Originated Disease TaiKangMedical School(School of Basic Medical Sciences)Wuhan UniversityWuhan 430071China Reproductive Medicine Center Zhongnan Hospital of Wuhan UniversityWuhan 430071China TaiKang Center for Life and Medical Sciences Wuhan UniversityWuhan 430071China

The selection of the most motile and functionally competent sperm is an essential basis for in vitro fertilization(IVF)and normal embryonic *** adopted clinical approaches for sperm sample processing intensely rely on centrifugation and wash steps that may induce mechanical damage and oxidative stress to *** a few microfluidic sperm sorting devices may avoid these adverse effects by exploiting intrinsic guidance mechanisms of sperm swimming,none of these approaches have been fully validated by clinical-grade assessment *** this study,a microfluidic sperm sorting device that enables the selection of highly motile and functional sperm via their intrinsic thermotaxis is *** by the temperature microenvironment in the fallopian tube during natural sperm selection,a microfluidic device with controllable temperature gradients along the sperm separation channel was designed and *** study investigated the optimal temperature conditions for human sperm selection and fully characterized thermotaxis-selected sperm with 45 human sperm *** indicated that a temperature range of 35–36.5℃along the separation channel significantly improves human sperm motility rate((85.25±6.28)%vs.(60.72±1.37)%;P=0.0484),increases normal sperm morphology rate((16.42±1.43)%vs.(12.55±0.88)%;P<0.0001),and reduces DNA fragmentation((7.44±0.79)%vs.(10.36±0.72)%;P=0.0485)compared to the nonthermotaxis *** thermotaxis is species-specific,and selected mouse sperm displayed the highest motility in response to a temperature range of 36–37.5℃ along the separation ***,IVF experiments indicated that the selected sperm permitted an increased fertilization rate and improved embryonic development from zygote to *** microfluidic thermotaxic selection approach will be translated into clinical practice to improve the IVF success rate for patients with oligozoospermia and asthenozoospermia.

关键词： Microfluidic device Thermotaxis Sperm sorting Assisted reproductive technology In vitro fertilization

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Fine mapping and cloning of the sterility gene Bra2Ms in nonheading Chinese cabbage(Brassica rapa ***)

引用

Journal of Integrative Agriculture 2024年第4期23卷 1195-1204页

作者： Liping Song Xia Li Liguang Tang Chuying Yu Bincai Wang Changbin Gao Yanfeng Xie Xueli Zhang Junliang Wang Chufa Lin Aihua Wang Wuhan Vegetable Research Institute Wuhan Academy of Agricultural SciencesWuhan 430345China Biotechnology and Genetic Resources Institute Yunnan Academy of Agricultural SciencesKunming 650000China

The application of a male-sterile line is an ideal approach for hybrid seed production in non-heading Chinese cabbage(Brassica rapa ***).However,the molecular mechanisms underlying male sterility in *** are still largely *** previously obtained the natural male sterile line WS24-3 of non-heading Chinese cabbage and located the male sterile locus,Bra2Ms,on the A2 *** observations revealed that the male sterility of WS24-3 resulted from disruption of the meiosis process during pollen *** mapping of Bra2Ms delimited the locus within a physical distance of about 129 kb on the A2 chromosome of *** Bra039753 gene encodes a plant homeodomain(PHD)-finger protein and is considered a potential candidate gene for ***039753 was significantly downregulated in sterile line WS24-3 compared to the fertile line at the meiotic anther *** analysis of Bra039753 identified a 369 bp fragment insertion in the first exon in male sterile plants,which led to an amino acid insertion in the Bra039753 *** addition,the 369 bp fragment insertion was found to cosegregate with the male sterility *** study identified a novel locus related to male sterility in non-heading Chinese cabbage,and the molecular marker obtained in this study will be beneficial for the marker-assisted selection of excellent sterile lines in non-heading Chinese cabbage and other Brassica crops.

关键词： non-heading Chinese cabbage male sterility Bra2Ms fine mapping PHD-finger protein

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

CdS/LaFeO_(3) heterojunctions with improved optical properties for visible-light catalytic applications

引用

Journal of Rare Earths 2024年第5期42卷 879-888,I0003页

作者： Jiaqi Wei Wen Zhu Xuelian Xu Xiao Xu Junjiang Zhu hubei key Laboratory of Biomass Fibers and Eco-dyeing&Finishing College of Chemistry and Chemical EngineeringWuhan Textile University ChemistryWuhan430200China

Construction of heterojunctions is a normal and effective strategy to improve the photocatalytic performances of semiconductors,through which both the lifetime and the redox ability of electrons/holes can be improved,as co mpared to the respective *** this basis,we constructed Z-scheme CdS/LaFeO_(3) heterojunctions(CdS/LFO),by in-situ growing different amounts of CdS on the surface of LaFeO_(3),for photocatalytic degradation of tetracycline hydrochloride(TC)in aqueous solution at room *** crystal structure,surface morphology and optoelectronic properties of the COS/LFO heterojunctions were systemically characterized to correlate the reaction *** tests indicate that the CdS/LFO heterojunctions exhibit promising activity for TC degradation under visible light irradiation(λ≥420 nm),with 85%TC conversion obtained at reaction time of 45 min,which is 3.4 and 1.9 times higher than that of CdS and LaFeO_(3).The CdS/LFO heterojunctions are also stable in the reaction and can be reused for four cycles with no appreciable activity *** applicability of CdS/LFO to photocatalytic degradation of organic dyes,as well as the reaction mechanism,was also explored.

关键词： LaFeO_(3) CdS Photocatalytic degradation Tetracycline hydrochloride Reaction mechanism Rare earths

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Experimental and numerical study on protective effect of RC blast wall against air shock wave

引用

Defence Technology（防务技术） 2024年第1期31卷 567-579页

作者： Xin-zhe Nian Quan-min Xie Xin-li Kong Ying-kang Yao Kui Huang State key Laboratory of Precision Blasting Jianghan UniversityWuhan430056China hubei key Laboratory of Blasting Engineering Jianghan UniversityWuhan430056China hubei(Wuhan)Institute of Explosion and Blasting Technology Jianghan UniversityWuhan430056China College of Defense Engineering Army Engineering University of PLANanjing210007China Institute of Defense Engineering AMSPLALuoyang471023China

Prototype experiments were carried out on the explosion-proof performance of the RC blast *** mass of TNT detonated in the experiments is 5 kg and 20 kg *** shock wave overpressure was tested in different *** above experiments were numerically simulated,and the simulated shock wave overpressure waveforms were compared with that tested and given by CONWEP *** results show that the numerically simulated waveform is slightly different from the test waveform,but similar to CONWEP *** dimensional analysis and numerical simulation under different working conditions,the equation for the attenuation rate of the diffraction overpressure behind the blast wall was *** to the corresponding standards,the degree of casualties and the damage degree of the brick concrete building at a certain distance behind the wall can be determined when parameters are *** above results can provide a reference for the design and construction of the reinforced concrete blast wall.

关键词： Blast wall Shock wave Diffraction Overpressure Protection

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Preparation and Analysis of Carbon Fiber-Silicon Carbide Thermally Conductive Asphalt Concrete

引用

Fluid Dynamics & Materials Processing 2024年第4期20卷 705-723页

作者： Zhiyong Yang Enjie Hu Lei Xi Zhi Chen Feng Xiong Chuanhai Zhan School of Civil Engineering and Environment Hubei University of TechnologyWuhan430068China

An experimental investigation into the thermal conductivity of CF-SiC two-phase composite asphalt concrete is *** main objective of this study was to verify the possibility of using SiC powder instead of mineral powder as the thermal conductive filler to prepare a new type of asphalt concrete and improve the efficiency of electrothermal snow and ice melting systems *** thermal conductivity of asphalt concrete prepared with different thermally conductive fillers was tested by a transient plane source method,and the related performances were *** the temperature rise rate and surface temperature were studied through field heating ***,the actual ice melting efficiency of the thermally conductive asphalt concrete was evaluated using an effective electrothermal *** shown by the experimental results,the composite made of SiC powder and carbon fiber has a high thermal *** SiC replaces mineral powder,the thermal conductivity of the asphalt mixture increases first and then decreases with the increase of carbon fiber *** the present study,in particular,the thermal conductivity attained a peak when the carbon fiber content was 0.2%of the aggregate mass.

关键词： Carbon fiber silicon carbide thermally conductive asphalt concrete road performance electrothermal snow melting

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Glass-Ceramics with High Strength and High Transmittance Obtained by Multi-Factor Controlling

引用

Journal of Wuhan University of Technology(Materials Science) 2024年第3期39卷 551-560页

作者：胡云坤 RAO Yu WANG Mingzhong XU Yinsheng XIA Mengling TAO Haizheng 陆平 School of Materials Science and Engineering Wuhan University of TechnologyWuhan 430070China Xianning CSG Photovoltaic Glass Co. Ltd.Xianning 437100China State key Laboratory of Silicate Materials for Architectures Wuhan University of TechnologyWuhan 430070China Jincheng Optical-mechanical Industry Research Institute Jincheng 048000China

The presence of Li_(2)Si_(2)O_(5) and LiAlSi_(4)O_(10) could effectively improve the elastic modulus and transmittance of lithium disilicate(LD)*** synergistically modulation of the crystal content and grain size,we obtained high strength and high transmittance of LD *** optimal sample had a high transmittance of 90.3%,the hardness was 7.72 GPa,the fracture toughness was 1.07 MPa·m^(1/2),and the elastic modulus was 103.1 GPa.

关键词： lithium disilicate glass-ceramics transmittance mechanical properties

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Influence of in situ formed spinel in bonding phase on mechanical properties and air permeability of magnesia-chrome porous purging materials

引用

Journal of Iron and Steel Research International 2024年第6期31卷 1459-1472页

作者： Yi An Hao Liu Zhou-fu Wang Yan Ma Xi-tang Wang The State key Laboratory of Refractories and Metallurgy Wuhan University of Science and TechnologyWuhan 430081HubeiChina

Magnesia-chrome porous purging plugs are crucial functional components to remove inclusions and stabilize the flow field during iron and steel ***,practical applications of magnesia-chrome porous purging materials are stil hampered by the poor scouring resistance to molten steel and unstable air permeability owing to their low mechanical properties and uncontrollable pore ***,the particle-packing type magnesiachrome porous purging materials reinforced by in situ formed spinel were prepared using fused magnesia-chrome particles and Al powders as major raw *** results show that in situ formed spinel solid solutions in bonding phase led to the decreased median pore size and increased pore surface fractal dimension from the reactions between Al powders and magnesia-chrome particles and along with high-temperature sintering,bonding between magnesia-chrome particles and the resultant mechanical properties of materials were greatly ***,the results of air permeability tests and polynomial ftting indicated that the formation of spinel solid solutions was the main contributing factor for controllable air permeability,and pressure drop of porous purging materials was positively correlated with surface fractal dimension of ***,the as-prepared porous purging materials added with 6 wt.%Al powders obtained the maximum cold crushing strength(54.2 MPa)and hot modulus of rupture(12.9 MPa)with median pore size of 24.06μm and ftting non-Darcian permeability coefficient of 0.97×10^(-6)m.

关键词： Magnesia Chrome porous purging material Metallic Al-Spinel Mechanical strength Air permeability

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Adenine nucleotide translocase 2(Ant2)is required for individualization of spermatogenesis of Drosophila melanogaster

引用

Insect Science 2024年第4期31卷 1055-1072页

作者： Zhen He Yang Fang Fengchao Zhang Yang Liu Xinkai Cheng Jiajia Wang Dechen Li Dengsong Chen Fan Wu Industrial Crops Institute Hubei Academy of Agriculture SciencesWuhanChina Institute of Evolution&Marine Biodiversity Ocean University of ChinaQingdaoShandong ProvinceChina

Successful completion of spermatogenesis is crucial for the perpetuation of the *** Drosophila,spermatid individualization,a process involving changes in mitochondrial structure and function is critical to produce functional mature ***2,encoding a mitochondrial adenine nucleotide translocase,is highly expressed in male testes and plays a role in energy metabolism in the ***,its molecular function remains ***,we identified an important role of Ant2 in spermatid *** Ant2 knockdown testes,spermatid individualization complexes composed of F-actin cones exhibited a diffuse distribution,and mature sperms were absent in the seminal vesicle,thus leading to male *** most striking effects in Ant2-knockdown spermatids were decrease in tubulin polyglycylation and disruption of proper mitochondria derivatives *** apoptotic cells were also observed in Ant2-knockdown *** further investigate the phenotype of Ant2 knockdown in testes at the molecular level,complementary transcriptome and proteome analyses were *** the mRNA level,868 differentially expressed genes were identified,of which 229 genes were upregulated and 639 were downregulated induced via Ant2 ***-labeling proteome analysis revealed 350 differentially expressed proteins,of which 117 proteins were upregulated and 233 were *** expression of glutathione transferase(GstD5,GstE5,GstE8,and GstD3),proteins involved in reproduction were significantly regulated at both the mRNA and protein *** results indicate that Ant2 is crucial for spermatid maturation by affecting mitochondrial morphogenesis.

关键词： adenine nucleotide translocase Drosophila melanogaster individualization complexes mitochondria spermatogenesis tubulin polyglycylation

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Research progress of heterogeneous structure magnesium alloys:A review

引用

Journal of Magnesium and Alloys 2024年第6期12卷 2147-2181页

作者： Xiang Chen Junlei Zhang Min Wang Weizhang Wang Di Zhao Haiming Huang Qi Zhao Xiaofei Xu Hongxia Zhang Guangsheng Huang hubei Longzhong Laboratory Wuhan University of Technology Xiangyang Demonstration ZoneXiangyang 441000China College of Materials Science and Engineering Hubei University of Automotive TechnologyShiyan 442002China College of Materials Science and Engineering Kunming University of Science and TechnologyKunming 650093China hubei key Laboratory of Energy Storage and Power Battery(hubei University of Automotive Technology) Shiyan 442002China College of Materials Science and Engineering Chongqing UniversityChongqing 400044China School of Mechanical Engineering Suzhou University of Science and TechnologySuzhou 215009China

In recent years,a new class of metallic materials featuring heterogeneous structures has *** materials consist of distinct soft and hard domains with significant differences in mechanical properties,allowing them to maintain high strength while offering superior ***(Mg)alloys,renowned for their low density,high specific strength,exceptional vibration damping,and electromagnetic shielding properties,exhibit tremendous potential as lightweight and functional *** their advantageous properties,high-strength Mg alloys often suffer from limited ***,the emergence of heterogeneous materials provides a fresh perspective for the development of Mg alloys with both high strength and *** article provided a fundamental overview of heterostructured materials and systematically reviewed the recent research progress in the design of Mg alloys with strength-ductility balance based on heterostructure *** review encompassed various aspects,including preparation methods,formation mechanisms of diverse heterostructures,and mechanical properties,both within domestic and international *** this basis,the article discussed the challenges encountered in the design and fabrication of heterostructured Mg alloys,as well as the urgent issues that require attention and resolution in the future.

关键词： Mg alloys Heterogeneous structure Processing techniques Strength and ductility Hetero-deformation induced stress Strain gradient

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Surface roughness classification using light scattering matrix and deep learning

引用

Science China(Technological Sciences) 2024年第2期67卷 520-535页

作者： SUN Hao TAN Wei RUAN YiXiao BAI Long XU JianFeng State key Laboratory of Intelligent Manufacturing Equipment and Technology School of Mechanical Science and EngineeringHuazhong University of Science and TechnologyWuhan 430074China Wuhan Digital Design and Manufacturing Innovation Center Co. LtdWuhan 430074China

High-quality optical components have been widely used in various applications;thus,extremely high beam quality is ***,surface roughness is a key indicator of the surface *** this study,the angular distribution of light scattering field intensity was obtained for surfaces having different roughness profiles based on the finite difference time domain(FDTD)method,and the results were compared with those obtained using the generalized Harvey-Shack(GHS)*** was shown that the FDTD approach can be used for an accurate simulation of the scattered field of a rough surface,and the superposition of results obtained from many surfaces that have the same roughness level was in good agreement with the result given by the analytic GHS model.A light scattering matrix(LSM)method was proposed based on the FDTD simulation results that could obtain rich surface roughness *** classification effect of LSM was compared with that of the single-incidence scattering distribution(SISD)based on a ResNet-50 deep learning *** classification accuracy of the model trained with the LSM dataset was obtained as 95.74%,which was 23.40%higher than that trained using the SISD ***,the effects of different noise types and filtering methods on the classification performance were analyzed,and the LSM was also shown to improve the robustness and generalizability of the trained surface roughness ***,the proposed LSM method has important implications for improving the data acquisition scheme of current light scattering measurement systems,and it also has the potential to be used for detection and characterization of surface defects of optical components.

关键词： surface roughness FDTD simulation GHS theory deep learning light scattering matrix

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：