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Structural Properties of Ions and Polyelectrolytes in Aqueous Solutions under External Electric Fields:The Sign Effect

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Chinese Journal of Polymer Science 2024年第9期42卷 1341-1352,I0007页

作者： Cheng-Jiang Lin Jun-Jun Wang Yuan Jiang Shu-Li Chen Hong-Fei Li Wen-Han Zhao Qing-Rong Huang Chang-Ru Rong Xiao-Zheng Duan State key laboratory of Polymer Physics and Chemistry Changchun Institute of Applied ChemistryChinese Academy of SciencesChangchun130022China School of Applied Chemistry and Engineering University of Science and Technology of ChinaHefei230026China National key laboratory of Advanced Vehicle Integration and Control China FAW Group Co.Ltd.Changchun130013China State key laboratory of molecular Engineering of Polymers Department of Macromolecular ScienceFudan UniversityShanghai200438China Department of Food Science Rutgers University65 Dudley RoadNew BrunswickNew Jersey08901USA

We utilize molecular dynamics simulations to investigate the microstructures of ions and polyelectrolytes in aqueous solutions under external electric *** focusing on the multi-body interactions between ionic components and H_(2)O molecules,as well as their responses to the external electric fields,we clarify several nontrivial molecular features of the ionic and polyelectrolyte solutions,such as the solvations of cations and anions,clustering of the ions,and dispersions/aggregations of polyelectrolyte chains,as well as the corresponding responses of H_(2)O molecules in these *** simulations illustrate the variations in structures of ionic solutions caused by reversing the charge sign of the ions,and elucidate the disparity in structures between anionic and cationic polyelectrolyte solutions in the presence of the external electric *** work clarifies the mechanism for the alternations in complex multi-body interactions in aqueous solutions caused by the application electric field,which can contribute to the fundamental understanding of the physical and chemical natures of ion-containing and charged polymeric systems.

关键词： Polyelectrolyte Ions Aqueous solutions External electric fields Asymmetric dipole

Ectopic Expression of TRIM25 Restores RIG-I Expression and IFN Production Reduced by Multiple Enteroviruses 3Cpro

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Virologica Sinica 2021年第6期36卷 1363-1374页

作者： Huimin Xiao Jingliang Li Xu Yang Zhaolong Li Ying Wang Yajuan Rui Bin Liu Wenyan Zhang Institute of virology and AIDS Research Key Laboratory of Organ Regeneration and Transplantation of The Ministry of EducationThe First Hospital of Jilin UniversityChangchun130021China Cancer Institute(key laboratory of Cancer Prevention and Intervention Ministry of Education)Second Affiliated HospitalSchool of MedicineZhejiang UniversityHangzhou310058China Department of Hand Surgery First Hospital of Jilin UniversityChangchun130021China Changchun Institute of Biological Products Co. LtdChangchun130012China

Enteroviruses(EVs) 3C proteins suppress type I interferon(IFN) responses mediated by retinoid acid-inducible gene I(RIG-I), while an E3 ubiquitin ligase, tripartite motif protein 25(TRIM25)-mediated RIG-I ubiquitination is essential for RIG-I antiviral activity. Therefore, whether the effect of EVs 3C on RIG-I is associated with TRIM25 expression is worth to be further investigated. Here, we demonstrate that 3C proteins of EV71 and coxsackievirus B3(CVB3) reduced not only RIG-I expression but also TRIM25 expression through protease cleavage activity, while overexpression of TRIM25 restored RIG-I expression and IFN-b production reduced by 3C proteins. Further investigation confirmed that the two amino acids and functional domains in TRIM25 required for RIG-I ubiquitination and TRIM25 structural conformation were essential for the recovery of RIG-I expression. Moreover, we also observed that TRIM25 could rescue RIG-I expression reduced by 3C proteins of CVA6 and EV-D68 but not CVA16. Our findings provide an insightful interpretation of 3C-mediated host innate immune suppression and support TRIM25 as an attractive target against multiple EVs infection.

关键词： 3C proteins RIG-I TRIM25 Innate immunity Enteroviruses(EVs)

EV713C protease cleaves host anti-viral factor OAS3 and enhances virus replication

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Virologica Sinica 2022年第3期37卷 418-426页

作者： Xiaolei Zhou Li Tian Jian Wang Baisong Zheng Wenyan Zhang Center for Infectious Diseases and Pathogen Biology Institute of Virology and AIDS ResearchKey Laboratory of Organ Regeneration and Transplantation of the Ministry of EducationThe First Hospital of Jilin UniversityJilin130021China

The global spread of enteroviruses(EVs)has become more frequent,severe and ***(IFN)I has been proved to control EVs by regulating IFN-stimulated genes(ISG)expression.20-50-oligoadenylate synthetases 3(OAS3)is an important ISG in the OAS/RNase L antiviral *** relationship between OAS3 and EVs is still ***,we reveal that OAS3,superior to OAS1 and OAS2,significantly inhibited EV71 replication in ***,EV71 utilized autologous 3C protease(3C^(pro))to cleave intracellular OAS3 and enhance viral ***,a human rhinovirus 3C protease inhibitor,completely abolished the cleavage of EV713C^(pro)on *** the proteolytically deficient mutants H40G,E71A,and C147G of EV713C^(pro)also lost the ability of OAS3 ***,the Q982-G983 motif in C-terminal of OAS3 was identified as a crucial 3C^(pro)cutting *** investigation indicated that OAS3 inhibited not only EV71 but also Coxsackievirus B3(CVB3),Coxsackievirus A16(CA16),Enterovirus D68(EVD68),and Coxsackievirus A6(CA6)***,unlike other four subtypes,CA163C^(pro)could not cleave *** key amino acids variation Ile36 and Val86 in CA163C^(pro)might result in weak and delayed virus replication of CA16 because of failure of OAS and 3AB *** works elucidate the broad anti-EVs function of OAS3,and illuminate a novel mechanism by which EV71 use 3C^(pro)to escape the antiviral effect of *** findings can be an important entry point for developing novel therapeutic strategies for multiple EVs infection.

关键词： Enteroviruses EV71 3C protease Cleavage Anti-viral factor OAS3

IFN-β1b induces OAS3 to inhibit EV71 via IFN-β1b/JAK/STAT1 pathway

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Virologica Sinica 2022年第5期37卷 676-684页

作者： Baisong Zheng Xiaolei Zhou Li Tian Jian Wang Wenyan Zhang Center for Pathogen Biology and Infectious Diseases Institute of Virology and AIDS ResearchKey Laboratory of Organ Regeneration and Transplantation of the Ministry of EducationThe First Hospital of Jilin UniversityChangchun130021China

Enterovirus 71(EV71) caused hand, foot and mouth disease(HFMD) is a serious threat to the health of young children. Although type I interferon(IFN-I) has been proven to control EV71 replication, the key downstream IFNstimulated gene(ISG) remains to be clarified and investigated. Recently, we found that 2’-5’-oligoadenylate synthetases 3(OAS3), as one of ISG of IFN-β1b, was antagonized by EV71 3C protein. Here, we confirm that OAS3is the major determinant of IFN-β1b-mediated EV71 inhibition, which depends on the downstream constitutive RNase L activation. 2’-5’-oligoadenylate(2-5A) synthesis activity deficient mutations of OAS3 D816A, D818A,D888A, and K950A lost resistance to EV71 because they could not activate downstream RNase L. Further investigation proved that EV71 infection induced OAS3 but not RNase L expression by IFN pathway. Mechanically, EV71 or IFN-β1b-induced phosphorylation of STAT1, but not STAT3, initiated the transcription of OAS3 by directly binding to the OAS3 promoter. Our works elucidate the immune regulatory mechanism of the host OAS3/RNase L system against EV71 replication.

关键词： Enterovirus 71(EV71) 20-50-oligoadenylate synthetases 3(OAS3) RNase L IFN-β1b JAK/STAT

恶嗪N-稠合咪唑-2-硫酮合成的机理研究:添加剂和溶剂的作用（英文）

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分子科学学报 2024年第3期 256-266页

作者： MOHAMED Hend 袁海艳张景萍东北师范大学化学学院

采用密度泛函理论计算了在无催化剂协助、水协助、三氟乙酸（TFA）协助条件下，2,2-二羟基-1-苯乙酮（1）,（2-氨基苯）甲醇（2）和KSCN（3）合成3-苯基-2,5-二氢-1-氢-苯并[d]咪唑并[5,1-b][1,3]恶嗪-1-硫酮的反应机理。计算结果表明，... 详细信息

采用密度泛函理论计算了在无催化剂协助、水协助、三氟乙酸（TFA）协助条件下，2,2-二羟基-1-苯乙酮（1）,（2-氨基苯）甲醇（2）和KSCN（3）合成3-苯基-2,5-二氢-1-氢-苯并[d]咪唑并[5,1-b][1,3]恶嗪-1-硫酮的反应机理。计算结果表明，TFA、水和底物2可以改变和促进该反应。基于底物不同的反应顺序共探究了3种机理M1（1+2然后与3反应）、M2（1+3然后与2反应）和M3（2+3然后与1反应）,并发现底物2的亲核性强于底物3。计算表明TFA-水共同协助机理M1的路径最优，决速步是[3+2]环加成过程。TFA和水分子在整个反应中起着至关重要的作用，通过N—H…O,O—H…O及C—H…O相互作用来稳定过渡态和中间体的结构。它们还作为氢键（HBs）给体和受体，通过改变乙二醛-水合物和KSCN的反应形式来提高底物的反应活性。底物2作为HBs受体促进了烯醇-酮互变异构和质子转移过程。进一步发现该反应可以用水替换CH3CN,为合成绿色恶嗪杂环类化合物提供了新的方案。

关键词：密度泛函理论反应机理添加剂和溶剂多组分反应

ATP1B3 Restricts Hepatitis B Virus Replication Via Reducing the Expression of the Envelope Proteins

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Virologica Sinica 2021年第4期36卷 678-691页

作者： Jun Zhang Tianhang Zheng Xiaolei Zhou Hong Wang Zhaolong Li Chen Huan Baisong Zheng Wenyan Zhang Institute of virology and AIDS Research the First Hospital of Jilin UniversityChangchun 130021China

Our recent study reported that ATP1B3 inhibits hepatitis B virus(HBV)replication via inducing NF-κB ***,ATP1B3 mutants which were defective in NF-κB activation still maintained the moderate degree of suppression on HBV replication,suggesting that another uncharacterized mechanism is also responsible for ATP1B3-mediated HBV ***,we demonstrated that ATP1B3 reduced the expression of HBV envelope proteins LHBs,MHBs and SHBs,but had no effect on intracellular HBV DNA,RNA levels as well as HBV promoter *** investigation showed that proteasome inhibitor MG132 rescued ATP1B3-mediated envelope proteins degradation,demonstrating that proteasome-dependent pathway is involved in ATP1B3-induced degradation of envelope ***-IP showed that ATP1B3 interacts with LHBs and MHBs and induces LHBs and MHBs *** colocalization analysis confirmed LHBs and MHBs colocalized with ATP1B3 *** work provides important information for targeting ATP1B3 as a potential therapeutic molecule for HBV infection.

关键词： ATP1B3 Hepatitis B virus(HBV) Envelope proteins Proteasome-dependent degradation

Regulation of Virus Replication and T Cell Homeostasis by N^6-Methyladenosine

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Virologica Sinica 2019年第1期34卷 22-29页

作者： Jing Yang Hong Wang Wenyan Zhang Institute of virology and AIDS Research The First Hospital of Jilin University

RNA modifications are abundant in eukaryotes, bacteria, and archaea. N^6-methyladenosine(m^6A), a type of RNA modification mainly found in messenger RNA(mRNA), has significant effects on the metabolism and function of m RNAs. This modification is governed by three types of proteins, namely methyltransferases as ‘‘writers' ', demethylases as ‘‘erasers' ',and specific m^6A-binding proteins(YTHDF1-3) as ‘‘readers' '. Further, it is important for the regulation of cell fate and has a critical function in many biological processes including virus replication, stem cell differentiation, and cancer development, and exerts its effect by controlling gene expression. Herein, we summarize recent advances in research on m^6A in virus replication and T cell regulation, which is a rapidly emerging field that will facilitate the development of antiviral therapies and the study of innate immunity.

关键词： RNA modification N^6-methyladenosine (m^6A) Virus replication T cell homeostasis

Pseudocapacitive sodium storage of Fe1-xS@N-doped carbon for low-temperature operation

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Science China Materials 2020年第4期63卷 505-515页

作者： Honghong Fan Bowen Qin Zhiwei Wang Huanhuan Li Jinzhi Guo Xinglong Wu Jingping Zhang National&Local United Engineering laboratory for Power Batteries Faculty of ChemistryNortheast Normal UniversityChangchun 130024China key laboratory for UV Light-Emitting Materials and Technology of Ministry of Education Northeast Normal UniversityChangchun 130024China

Constructing potential anodes for sodium-ion batteries(SIBs)with a wide temperature property has captured enormous interests in recent ***1-xS,a zero-band gap material confirmed by density states calculation,is an ideal electrode for fast energy storage on account of its low cost and high theoretical ***,Fe1-xS nanosheet wrapped by nitrogen-doped carbon(Fe1-xS@NC)is engineered through a post-sulfidation strategy using Fe-based metal-organic framework(Fe-MOF)as the *** obtained Fe1-xS@NC agaric-like structure can well shorten the charge diffusion pathway,and significantly enhance the ionic/electronic conductivities and the reaction *** expected,the Fe1-xS@NC electrode,as a prospective SIB anode,delivers a desirable capacity up to 510.2 mA h g^-1 at a high rate of8000 mA g^-***,even operated at low temperatures of 0 and-25°C,high reversible capacities of 387.1 and 223.4 mA h g^-1 can still be obtained at 2000 mA g^-1,respectively,indicating its huge potential use at harsh *** noticeably,the full battery made by the Fe1-xS@NC anode and Na3 V2(PO4)2 O2 F cathode achieves a remarkable rate capacity(186.8 mA h g^-1 at 2000 m A g^-1)and an impressive cycle performance(183.6 m A h g^-1 after 100 cycles at700 mA g^-1)between 0.3 and 3.8 *** excellent electrochemical performance is mainly contributed by its pseudocapacitive-dominated behavior,which brings fast electrode kinetics and robust structural stability to the whole electrode.

关键词： agaric-like structure sodium ion batteries Fe1-xS low-temperature performance full cells

Deubiquitinase ubiquitin-specific protease 3 (USP3) inhibits HIV-1 replication via promoting APOBEC3G (A3G) expression in both enzyme activity-dependent and -independent manners

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Chinese Medical Journal 2022年第22期135卷 2706-2717页

作者： Simin Zhao Baisong Zheng Liuli Wang Wenzhe Cui Chunlai Jiang Zhuo Li Wenying Gao Wenyan Zhang Center for Pathogen Biology and Infectious Diseases Institute of Virology and AIDS ResearchKey Laboratory of Organ Regeneration and Transplantation of The Ministry of EducationThe First Hospital of Jilin UniversityChangchunJilin 130021China College of Life Science of jilin University ChangchunJilin 130012China Department of Regenerative Medicine School of Pharmaceutical SciencesJilin UniversityChangchunJilin 130012China jilin Provincial key laboratory on molecular and Chemical Genetics The Second Hospital of Jilin UniversityChangchunJilin 130041China Department of Endocrinology and Metabolism The First Hospital of Jilin UniversityChangchunJilin 130021China

Background: Ubiquitination plays an essential role in many biological processes, including viral infection, and can be reversed by deubiquitinating enzymes (DUBs). Although some studies discovered that DUBs inhibit or enhance viral infection by various mechanisms, there is lack of information on the role of DUBs in virus regulation, which needs to be further ***: Immunoblotting, real-time polymerase chain reaction,in vivo/in vitro deubiquitination, protein immunoprecipitation, immunofluorescence, and co-localization biological techniques were employed to examine the effect of ubiquitin-specific protease 3 (USP3) on APOBEC3G (A3G) stability and human immunodeficiency virus (HIV) replication. To analyse the relationship between USP3 and HIV disease progression, we recruited 20 HIV-infected patients to detect the levels of USP3 and A3G in peripheral blood and analysed their correlation with CD4^(+) T-cell counts. Correlation was estimated by Pearson correlation coefficients (for parametric data).Results: The results demonstrated that USP3 specifically inhibits HIV-1 replication in an A3G-dependent manner. Further investigation found that USP3 stabilized 90% to 95% of A3G expression by deubiquitinating Vif-mediated polyubiquitination and blocking its degradation in an enzyme-dependent manner. It also enhances the A3G messenger RNA (mRNA) level by binding to A3G mRNA and stabilizing it in an enzyme-independent manner. Moreover, USP3 expression was positively correlated with A3G expression (r= 0.5110) and CD4^(+) T-cell counts (r= 0.5083) in HIV-1-infected ***: USP3 restricts HIV-1 viral infections by increasing the expression of the antiviral factor A3G. Therefore, USP3 may be an important target for drug development and serve as a novel therapeutic strategy against viral infections.

关键词： APOBEC3G Ubiquitin-specific protease 3 Deubiquitination Human immunodeficiency virus-1 Vif Human immunodeficiency virus Deubiquitinase