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The effect of SphK1/S1P signaling pathway on hepatic sinus microcirculation in rats with hepatic ischemia-reperfusion injury

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Hepatobiliary & Pancreatic Diseases International 2022年第1期21卷 94-98页

作者： Li-Ming Jin Yuan-Xing Liu Jian Cheng Lin Zhou Hai-Yang Xie Xiao-Wen Feng Hui Li Yan Shen Xiao Xu Shu-Sen Zheng Division of Hepatobiliary and Pancreatic Surgery Department of SurgeryFirst Affiliated HospitalZhejiang University School of MedicineHangzhou 310003China NHC Key Laboratory of Combined Multi-organ Transplantation Hangzhou 310003China Department of General Surgery Hepatobiliary and Pancreatic Surgery and Minimally Invasive SurgeryZhejiang Provincial People’s HospitalAffiliated People’s Hospital of Hangzhou Medical CollegeHangzhou 310014China

To the Editor:Hepatic ischemia-reperfusion(I/R)injury is the component of liver injury related to liver transplantation and liver surgery[1-3].The hepatic sinus microcirculation injury is a central part of hep-atic I/R injury,which eventually leads to hepatocyte *** process is closely associated with the participation of liver non-parenchymal cells such as Kuffer cells,hepatic stellate cells,liver sinusoidal endothelial cells(LSECs),and cytokines.

关键词： hepatic ischemia microcirculation

BMI-1 activates hepatic stellate cells to promote the epithelialmesenchymal transition of colorectal cancer cells

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World Journal of Gastroenterology 2023年第23期29卷 3606-3621页

作者： Zhong-Yang Jiang Xi-Mei Ma Xiao-Hui Luan Zhen-Yu Liuyang Yi-Yang Hong Yuan Dai Qing-Hua Dong Guan-Yu Wang Department of General Surgery Sir Run Run Shaw HospitalSchool of MedicineZhejiang UniversityHangzhou 310016Zhejiang ProvinceChina Department of Emergency The Second Affiliated Hospital of Zhejing UniversityHangzhou 310016Zhejiang ProvinceChina Biomedical Research Center Sir Run Run Shaw HospitalSchool of MedicineZhejiang UniversityHangzhou 310016Zhejiang ProvinceChina Key Laboratory of Cancer Prevention and Intervention China National Ministry of EducationHangzhou 310009Zhejiang ProvinceChina

BACKGROUND Activated hepatic stellate cells(aHSCs)are the major source of cancer-associated fibroblasts in the *** the crosstalk between aHSCs and colorectal cancer(CRC)cells supports liver metastasis(LM),the mechanisms are largely *** To explore the role of BMI-1,a polycomb group protein family member,which is highly expressed in LM,and the interaction between aHSCs and CRC cells in promoting CRC liver metastasis(CRLM).METHODS Immunohistochemistry was carried out to examine BMI-1 expression in LM and matched liver specimens of *** expression levels of BMI-1 in mouse liver during CRLM(0,7,14,21,and 28 d)were detected by Western blotting(WB)and the quantitative polymerase chain reaction(qPCR)*** overexpressed BMI-1 in HSCs(LX2)by lentivirus infection and tested the molecular markers of aHSCs by WB,qPCR,and the immunofluorescence *** cells(HCT116 and DLD1)were cultured in HSC-conditioned medium(LX2 NC CM or LX2 BMI-1 CM).CM-induced CRC cell proliferation,migration,epithelial-mesenchymal transition(EMT)phenotype,and transforming growth factor beta(TGF-β)/SMAD pathway changes were investigated in vitro.A mouse subcutaneous xenotransplantation tumor model was established by co-implantation of HSCs(LX2 NC or LX2 BMI-1)and CRC cells to investigate the effects of HSCs on tumor growth and the EMT phenotype in *** Positive of BMI-1 expression in the liver of CRLM patients was 77.8%.The expression level of BMI-1 continued to increase during CRLM in mouse liver ***2 overexpressed BMI-1 was activated,accompanied by increased expression level of alpha smooth muscle actin,fibronectin,TGF-β1,matrix metalloproteinases,and interleukin *** cells cultured in BMI-1 CM exhibited enhanced proliferation and migration ability,EMT phenotype and activation of the TGF-β/SMAD *** addition,the TGF-βR inhibitor SB-505124 diminished the effect of BMI-1 CM on SMAD2/3 phosphorylation in CRC ***,BMI-1 overexpressed LX2 HSCs promot

关键词： BMI-1 Hepatic stellate cells Colorectal cancer Liver metastasis Epithelial-mesenchymal transition

5′-tiRNA-Gln inhibits hepatocellular carcinoma progression by repressing translation through the interaction with eukaryotic initiation factor 4A-I

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Frontiers of Medicine 2023年第3期17卷 476-492页

作者： Chengdong Wu Dekai Liu Lufei Zhang Jingjie Wang Yuan Ding Zhongquan Sun Weilin Wang Department of Hepatobiliary and Pancreatic Surgery The Second Affiliated HospitalZhejiang University School of MedicineHangzhou 310009China Key Laboratory of Precision Diagnosis and Treatment for Hepatobiliary and Pancreatic Tumor of zhejiang province Hangzhou 310009China Research Center of Diagnosis and Treatment Technology for Hepatocellular Carcinoma of zhejiang province Hangzhou 310009China Clinical Medicine Innovation Center of Precision Diagnosis and Treatment for Hepatobiliary and Pancreatic Disease of zhejiang University Hangzhou 310009China Clinical Research Center of Hepatobiliary and Pancreatic Diseases of zhejiang province Hangzhou 310009China Cancer Center Zhejiang UniversityHangzhou 310009China

tRNA-derived small RNAs(tsRNAs)are novel non-coding RNAs that are involved in the occurrence and progression of diverse ***,their exact presence and function in hepatocellular carcinoma(HCC)remain ***,differentially expressed tsRNAs in HCC were profiled.A novel tsRNA,tRNAGln-TTG derived 5′-tiRNA-Gln,is significantly downregulated,and its expression level is correlated with progression in *** HCC cells,5′-tiRNA-Gln overexpression impaired the proliferation,migration,and invasion in vitro and in vivo,while 5′-tiRNA-Gln knockdown yielded opposite results.5′-tiRNA-Gln exerted its function by binding eukaryotic initiation factor 4A-I(EIF4A1),which unwinds complex RNA secondary structures during translation initiation,causing the partial inhibition of *** suppressed downregulated proteins include ARAF,MEK1/2 and STAT3,causing the impaired signaling pathway related to HCC ***,based on the construction of a mutant 5′-tiRNA-Gln,the sequence of forming intramolecular G-quadruplex structure is crucial for 5′-tiRNA-Gln to strongly bind EIF4A1 and repress ***,5′-tiRNA-Gln expression level is negatively correlated with ARAF,MEK1/2,and STAT3 in HCC ***,these findings reveal that 5′-tiRNA-Gln interacts with EIF4A1 to reduce related mRNA binding through the intramolecular Gquadruplex structure,and this process partially inhibits translation and HCC progression.

关键词： EIF4A1 G-quadruplex hepatocellular carcinoma tRNA-derived small RNA translation initiation

Clinical practice and implementation of enhanced recovery programs in hepato-pancreato-biliary surgery

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Hepatobiliary Surgery and Nutrition 2018年第6期7卷 492-494页

作者： Liming Chen Lixia Cai Beilei Gao Xiangying Zhang Division of Hepatobiliary Pancreatic Surgery The First Affiliated HospitalZhejiang University School of MedicineHangzhou 310003China

The concept of enhanced recovery after surgery(ERAS)is developed according to the evidence-based *** reduces the physiological and psychological stress in surgical *** aims at expediting postoperative recovery by implementing specific strategies in perioperative *** optimizes the perioperative care through the multidisciplinary collaboration of surgeons,anesthesiologists,nurses and nutritionists;reduces the perioperative stress response and postoperative complications;shortens the hospital stay,and promotes the patient rehabilitation(1).

关键词： surgery nurse collaboration

Crotalaria ferruginea extract attenuates lipopolysaccharide-induced acute lung injury in mice by inhibiting MAPK/NF-κB signaling pathways

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Asian Pacific Journal of Tropical Biomedicine 2021年第11期11卷 481-490页

作者： Wei Pan Li-Ping Meng Jie Su Zheng-Biao Yang Wei-Feng Du Zhi-Wei Xu Yun-Xiang Chen Sheng Zhang Feng Xie Cong Xu Hong-Zhong Yang Wei-Hong Ge Department of Pharmacology College of Pharmaceutical SciencesZhejiang Chinese Medical UniversityHangzhou 310053China State Key Laboratory of Safety Evaluation for New Drugs Hangzhou Medical CollegeHangzhou 310013China Department of Surgical Nursing The First Affiliated Hospital of Hainan Medical UniversityHaikou 570102China

Objective:To evaluate the anti-inflammatory activity of Crotalaria ferruginea extract(CFE)and its ***:An intratracheal lipopolysaccharide(LPS)instillationinduced acute lung injury(ALI)model was used to study the antiinflammatory activity of CFE in *** LPS-induced shock model was used to analyze the effect of CFE on ***-stimulated RAW264.7 cell model was used to investigate the anti-inflammatory activity of CFE in vitro and the effects on mitogen-activated protein kinase(MAPK)or nuclear factor-κB(NF-κB)signaling ***:CFE administration decreased the number of inflammatory cells,reduced the levels of tumor necrosis factor-α(TNF-α),monocyte chemotactic protein-1(MCP-1),interleukin-6(IL-6),and interferon-γ,and diminished protein content in the bronchoalveolar lavage fluid of *** also reduced lung wet-to-dry weight ratio,myeloperoxidase,and lung tissue pathological *** preadministration improved the survival rate of mice challenged with a lethal dose of *** reduced LPS-activated RAW264.7 cells to produce nitric oxide,TNF-α,MCP-1,and ***,CFE inhibited nuclear translocation and phosphorylation of NF-κB P65,extracellular signal-regulated kinase,c-Jun N-terminal kinases,and P38 ***:CFE exhibits potent anti-inflammatory activity in LPS-induced ALI mice,LPS-shock mice,and RAW264.7 cells,and its mechanism may be associated with the inhibition of NF-κB and MAPK signaling *** ferruginea may be a useful therapeutic drug for the treatment of ALI and other respiratory inflammations.

关键词： Crotalaria ferruginea Acute lung injury Cytokine Lipopolysaccharide Nuclear factor-κB Mitogen-activated protein kinase

Application of extracorporeal membrane oxygenation in the endoscopic treatment of severe benign airway stenosis:A case report and literature review

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Laparoscopic, Endoscopic and Robotic Surgery 2021年第2期4卷 48-52页

作者： Yuyuan Liang Jihong Zhu Xing Zheng Qing Qiao Yaolin Guan Jisong Zhang Enguo Chen Department of Anesthesia Sir Run Run Shaw HospitalZhejiang University School of MedicineHangzhou 310016China Department of Anesthesia People's Hospital of Qingyuan CountyLishui 323800China Department of Respiratory Sir Run Run Shaw HospitalZhejiang University School of MedicineHangzhou 310016China

Ventilation strategies in patients with severe tracheal stenosis should be tailored to the patient according to the underlying cause and narrowing *** report is on a case of a 68-year-old male patient,who was admitted for radiotherapy because of esophageal cancer and then developed severe stenosis at the cervical *** used venovenous extracorporeal membrane oxygenation to secure the airway and ensure adequate *** urgent endoscopic balloon dilation of airway stenosis was successfully performed under general *** case shows that venovenous extracorporeal membrane oxygenation can be used in endoscopic tracheal procedures for patients with severe benign stenosis in the upper-trachea who are unable to tolerate conventional ventilation.

关键词： Severe tracheal stenosis Venovenous extracorporeal membrane oxygenation Ventilation strategy

Paraplegia after transcatheter artery chemoembolization in a child with clear cell sarcoma of the kidney:A case report

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World Journal of Clinical Cases 2020年第11期8卷 2332-2338页

作者： Jia-Bin Cai Min He Fa-Liang Wang Jie-Ni Xiong Jun-Qing Mao Zhong-Hai Guan Lin-Jie Li Jin-Hu Wang Department of Surgical Oncology The Children’s HospitalZhejiang University School of MedicineNational Clinical Research Center for Child HealthHangzhou 310053Zhejiang ProvinceChina

BACKGROUND Transcatheter arterial chemoembolization(TACE)is a common treatment for inoperable malignant renal ***,a series of complications may follow the TACE *** cord injury caused by the embolization of intercostal or lumbar arteries is extremely *** SUMMARY We describe a case with quite uncommon spinal cord injury after TACE in a 3-year-old child with clear cell sarcoma of the *** impairment beneath the T10 dermatomes and paraplegia on the day after TACE were found in this ***,sustained paraplegia still existed for more than 2 mo after TACE despite the large dose of steroids and supportive *** We should draw attention to an uncommon complication of paraplegia after TACE treatment in malignant renal *** it is rare,the result is disastrous.

关键词： Complication Chemoembolization Spinal cord injury Clear cell sarcoma of the kidney Pediatric Case report

A review of functional slit lamp biomicroscopy

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Eye and Vision 2019年第1期6卷 105-113页

作者： Xupeng Shu Jianhua Wang Liang Hu School of Ophthalmology and Optometry Wenzhou Medical UniversityWenzhou 325027China Bascom Palmer Eye Institute University of MiamiMiamiFloridaUSA

Functional slit lamp biomicroscopy(FSLB)is a novel device which consists of a traditional slit-lamp and a digital *** can quantitatively assess vessel diameter,blood flow velocity,and blood flow rate and can create noninvasive microvascular perfusion maps(nMPMs).At present,FSLB is mainly used in contact lens(CL)and dry eye disease(DED)studies to advance our understanding of ocular surface ***-derived blood flow and vessel density measures are significantly altered in CL wearers and DED patients compared to normal *** subtle changes in the ocular surface microcirculation may contribute to the monitoring of potential diseases of the body and provide a new way to diagnose dry eye ***,this may also indicate that FSLB can be more widely applied in the study of other diseases to reveal the relationship between changes in ocular surface microcirculation and systemic *** purpose of this paper is to summarize the functions of FSLB and the related studies especially in CL and DED.

关键词： Functional slit lamp biomicroscopy(FSLB) Conjunctival blood flow velocity Microvascular network density Contact lens Dry eye disease

Identification of Pulmonary Hypertension Animal Models Using a New Evolutionary Machine Learning Framework Based on Blood Routine Indicators

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Journal of Bionic Engineering 2023年第2期20卷 762-781页

作者： Jiao Hu Shushu Lv Tao Zhou Huiling Chen Lei Xiao Xiaoying Huang Liangxing Wang Peiliang Wu Department of Computer Science and Artificial Intelligence Wenzhou UniversityWenzhou 325035People'sRepublicof China Department of Dermatology Beijing Tongren HospitalCapital Medical UniversityBeijing 100730People's Republicof China The First Clinical College Wenzhou Medical UniversityWenzhou 325000People's Republic of China Department of Pulmonary and Critical Care Medicine The First Affiliated Hospital of Wenzhou Medical UniversityWenzhou 325000People's Republic of China

Pulmonary Hypertension(PH)is a global health problem that affects about 1%of the global *** models of PH play a vital role in unraveling the pathophysiological mechanisms of the *** present study proposes a Kernel Extreme Learning Machine(KELM)model based on an improved Whale Optimization Algorithm(WOA)for predicting PH mouse *** experimental results showed that the selected blood indicators,including Haemoglobin(HGB),Hematocrit(HCT),Mean,Platelet Volume(MPV),Platelet distribution width(PDW),and Platelet–Large Cell Ratio(P-LCR),were essential for identifying PH mouse models using the feature selection method proposed in this ***,the method achieved 100.0%accuracy and 100.0%specificity in classification,demonstrating that our method has great potential to be used for evaluating and identifying mouse PH models.

关键词： Feature selection Pulmonary hypertension Whale optimization algorithm Extreme learning machine