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Using liquid chromatography tandem mass spectrometry to detect rhodamine123 in rat plasma

Using liquid chromatography tandem mass spectrometry to dete...

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第十三次全国临床药理学学术大会

作者： Ya-Bin Sun~a,Zhong-Yuan Xu~a,Guo-Feng Li~b a GCP Office,Nanfang Hospital,Southern Medical University,guangzhou 510515,china b Department of Pharmacy,Nanfang Hospital,Southern Medical University,guangzhou 510515,china

A new,rapid and sensitive method was developed for the quantifying of rhodamine 123(R123) in rat plasma using liquid chromatography tandem mass spectrometry(LC-MS/MS).Rhodamine 6G(R6G) was used as the internal standard(IS).R123 and IS were extracted from aliquots of plasma with ethyl acetate and dichloromethane (4:1) as the *** separation was performed using a Zorbax Eclipse Plus C18 *** mobile phase was composed of A:ammonium formate-formic acid buffer containing 5 mM ammonium formate and 0.1%formic acid and B:methanol(A:B,5:95,v/v).To quantify R123 and IS respectively,multiple reaction monitoring(MRM) transition of m/z 345.2→285.2 and m/z 443.3→415.2 were *** analysis lasted for 4 min,in the positive mode,the results of which were presented as a linear calibration curve over the concentration range of 1-200 ng/ml and sampling volume of 2μ*** lowest limit of quantification(LLOQ) reached 1 ng/*** intra and inter-day precision were 6.7%and 9.2%for the low quality control(QC) sample (2 ng/ml),*** and inter-day precision of the medium QC sample(100 ng/ml) were both less than 3.4%.Intra and inter-day precision of high QC sample(150 ng/ml) were both less than 2.1%,while the intra and inter-day relative errors ranged between -7.4%and 9.1%for the above three QC concentration *** LC-MS/MS method proved to be simple,accurate,reliable and with a shorter running time and has been successfully applied to an absorption experiment in the rat.

关键词： Rhodamine 123 Liquid chromatography tandem mass spectrometry Rhodamine 6G Rat plasma

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