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Genetic Diversity Analysis of Rice Germplasm in Tripura State of Northeast India Using Drought and Blast Linked Markers

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Rice science 2017年第1期24卷 10-20页

作者： Alpana ANUPAM Jahangir IMAM Syed Mohammad QUATADAH Anantha SIDDAIAH Shankar Prasad DAS Mukund VARIAR Nimai Prasad MANDAL Central Rainfed Upland Rice Research Station ICAR Research Complex for North Eastern Hill Region Tripura Centre

We genotyped 74 rice germplasms including Tripura＇s local landraces, improved varieties, cultivars and breeding lines and other rice varieties using molecular markers for genetic diversity, drought QTLs, and blast resistance genes. The number of alleles per locus ranged from 2 to 5 with an average of 2.9. The polymorphic information content value per locus ranged from 0.059（RM537） to 0.755（RM252） with an average of 0.475. Cluster analysis based on 30 simple sequence repeat markers revealed 5 clusters and also indicated the presence of variability within the rice accessions. The drought QTL q DTY2.1 was found in 56.0% of germplasms and q DTY1.1 was detected in only 6.8% of the germplasms. Out of seven rice blast resistance genes screened, only two rice varieties, RCPL-1-82 and Buh Vubuk（Lubuk）, were positive for four blast resistance genes while only Releng possessed two blast resistance genes. Among 74 rice germplasms, only three accessions, Releng, RCPL1-82 and Buh Vubuk（Lubuk）, possessed both drought-related QTLs and blast resistance genes. Overall, the 74 indigenous rice genotypes showed low level of genetic diversity, which is in contrast to high level of genetic diversity among rice varieties in northeast India, where highlights the good farming practice, conservation of germplasms and the limitation of molecular markers employed in this study. The presence of both drought related QTLs and blast resistance genes in some of the germplasms can be useful in future breeding programmes.

关键词： drought blast alleles breeding ranged polymorphic Northeast screened upland germplasm

Compression Molded Ultra High Molecular Weight Polyethylene-Hydroxyapatite-Aluminum Oxide-Carbon Nanotube Hybrid Composites for Hard Tissue Replacement

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Journal of Materials Science & Technology 2013年第6期29卷 514-522页

作者： Ankur Gupta Garima Tripathi Debrupa Lahiri Kantesh Balani department of Materials Science and Engineering Indian Institute of Technology KanpurKanpur 208016India department of Mechanical and Materials Engineering.Florida International University MiamiFL 33174USA

Ultra high molecular weight polyethylene （UHMWPE） is widely used for articulating surfaces in total hip and knee replacements. In the present work, UHMWPE based polymer composites were synthesized by synergistic reinforcing of bioactive hydroxyapatite （HA）, bioinert aluminum oxide （Al2O3）, and carbon nanotubes （CNTs） using compression molding. Phase and microstructural analysis suggests retention of UHMWPE and reinforcing phases in the compression molded composites. Microstructural analysis elicited variation in densification due to the size effect of the reinforcing particles. The hybrid composites exhibited hardness, elastic modulus and toughness comparable to that of UHMWPE. The interfacial effect of reinforcement phases has evinced the effectiveness of Al2O3 over HA and CNT reinforcements, depicting synergistic enhancement in hardness and elastic modulus. Weak interfacial bonding of polymer matrix with HA and CNT requires utilization of coupling agents to achieve enhanced mechanical properties without deteriorating cytocompatible properties.

关键词： Compression molding Ultra high molecular weight polyethylene (UHMWPE) Hydroxyapatite Aluminum oxide (AI2O3) Carbon nanotubes (CNTs) Nanoindcntation

Effect of Porous Activated Charcoal Reinforcement on Mechanical and In-Vitro Biological Properties of Polyvinyl Alcohol Composite Scaffolds

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Journal of Materials Science & Technology 2017年第7期33卷 734-743页

作者： Tejinder Kaur Arunachalam Thirugnanam department of biotechnology and Medical Engineering National Institute of Technology Rourkela Odisfla 769008 Indi

The present work focused on developing an innovative composite material by reinforcing polymer matrix with highly porous activated charcoal. Polyvinyl alcohol-activated charcoal（PVA-AC） composite scaffolds were developed by varying the AC concentrations（0, 0.5, 1, 1.5, 2 and 2.5 wt%） in PVA matrix by freeze drying method. The developed scaffolds were characterized for their physicochemical, mechanical and in-vitro biological properties. In addition, the effect of AC on the attachment, proliferation and differentiation of osteoblast MG 63 cells was evaluated by scanning electron microscopy（SEM）, 3-（4,5-dimethylthiazol-2-yl）-2,5-diphenyltetrazolium bromide（MTT） assay, alkaline phosphatase（ALP） activity assay and alizarin red stain-based（ARS） assay. All the PVA-AC composite scaffolds exhibited good bioactivity, hemocompatibility and protein adsorption properties. The scaffolds with high AC concentration（2.5 wt%） showed controlled drug release kinetics that are suitable for long term healing. The mechanical properties of all the PVA-AC composite scaffolds were improved when compared to the pure PVA scaffold. The high porosity, swelling degree and hydrophilicity of PVA-AC composite scaffolds facilitated cell attachment and proliferation. This is due to porous AC present in the sample that supported the osteoblast differentiation and formed mineralized nodules without the addition of any extra agents. From the above studies, it can be concluded that PVA-AC composite scaffolds are promising biomaterials for bone tissue engineering applications.

关键词： Polyvinyl alcohol Activated charcoal Porous reinforcement Bioactivity Mechanical studies Osteoblast proliferation and differentiation

Genotypic Variation in Spatial Distribution of Fe in Rice Grains in Relation to Phytic Acid Content and Ferritin Gene Expression

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Rice science 2020年第3期27卷 227-236页

作者： Anurag MISHRA MdSHAMIM MdWASIM Siddiqui Akanksha SINGH Deepti SRIVASTAVA K.N.SINGH department of Plant Molecular Biology and Genetic Engineering Narendra Deva University of Agriculture and TechnologyKumarganjFaizabadUttar Pradesh 224229India Division of Genetics ICAR-Indian Agricultural Research InstituteNew Delhi 110012India department of Molecular Biology and Genetic Engineering Dr.Kalam Agricultural CollegeKishanganj(Bihar Agricultural UniversitySabourBhagalpur)Bihar 855107India department of Post-Harvest Technology Bihar Agricultural UniversitySabourBhagalpurBihar 813210India Integral Institute of Agricultural Science and Technology(IIAST)Integral University Kursi RoadDasauliUttar Pradesh 226026India

Rice varieties having high Fe concentration in the endospermic region can be used as a good source for Fe deficit *** this study,303 Oryza sativa varieties and 1 Oryza rufipogon accession were assessed for spatial Fe accumulation in grains by Prussian blue staining *** ferritin protein distribution in grains was visualized by immunohistochemistry,and ferritin expression was assessed in selected rice varieties using semi-quantitative reverse transcription *** popular rice varieties,namely Sarjoo 52,Madhukar and Jalmagna,and the *** variety showed Fe in all the regions of grains,and the highest Fe concentration was observed in the embryo *** high-yielding varieties like Swarna,Swarna Sub 1,CSR13 and NDRR359 had lower Fe concentration in the embryo *** highest Fe concentration was detected in ***(49.8μg/g),followed by Sarjoo 52(26.1μg/g)and Madhukar(25.7μg/g).Phytic acid concentration was the minimum in ***(5.75 mg/g)followed by Sarjoo 52(5.83 mg/g).Western blot and semi-quantitative reverse transcription PCR showed higher expression of ferritin gene in ***,Sarjoo 52 and *** conclusion,*** and Sarjoo 52 had higher Fe concentration in the embryo regions as well as endosperm and aleurone layer,whereas the other varieties had lower Fe concentration in the *** 52 could be used as a donor in the rice breeding program for the generation of new varieties with elevated grain Fe concentration.

关键词： bio-fortification iron accumulation ferritin tissue-specific localization iron deficit population

Erosion corrosion control of 6061 aluminum alloy in multi-phase jet impingement conditions with eco-friendly green inhibitor

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Chinese Journal of Chemical Engineering 2020年第2期28卷 340-347页

作者： M.Lavanya V.Ramachandra Murthy Padmalatha Rao department of biotechnology Manipal Institute of TechnologyManipal Academy of Higher EducationManipalKarnatakaIndia department of Chemistry Manipal Institute of TechnologyManipal Academy of Higher EducationManipalKarnatakaIndia

Erosion corrosion performance of 6061 aluminum alloy in simulated sea water slurry was investigated under multi-phase jet impingement conditions. The main objective of the work is to study erosion–corrosion of a material with engineering application and mitigate it using eco-friendly green inhibitor. Experiments were performed with the sand concentration of 0.3% and 0.3 μm size. The effect of temperature and flow rate on the performance of inhibitor was explored. Electrochemical methods were adopted for erosion-corrosion measurements. Experiments revealed that starch could mitigate erosion-corrosion with a maximum reduction efficiency of 58% at temperature of 303 K and flow rate of 4 L·min–1. Inhibition efficiency decreased with increase in flowrate and temperature. EIS spectrum demonstrated that the corrosion process in the presence on inhibitor was both charge transfer and mass transfer controlled. A key role of hydrodynamics in the performance of corrosion inhibitor was confirmed by the present study.

关键词： Erosion-corrosion Green-inhibitor Electrochemical studies Aluminum alloy Simulated sea water

Bio-interface behaviour of graphene and semiconducting SWCNT:C60 blend based nano photodiode for subretinal implant

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Biosurface and Biotribology 2020年第2期6卷 53-58页

作者： Moorthy Vijai Meyyappan Varadarajan Parthasarathy Rathnasami Joseph Daniel NPMaSS MEMS Design Centre Department of Electronics and Instrumentation EngineeringFaculty of Engineering&TechAnnamalai UniversityAnnamalainagar608002TamilnaduIndia Centre for cell Biology and Drug Discovery Faculty of Engineering&TechAnnamalai UniversityAnnamalainagar608002TamilnaduIndia

Hybrid interfaces between living cells and organic conjugated polymers play a pivotal role in bioelectronics ***,conjugated polymers are widely utilised for optical stimulation of living cells and other bio-interface applications such as neural probes,cellular scaffolds and biosensors for drug *** such a work,the authors fabricated and characterised the Nano Photodiode array device for the subretinal ***,the authors did not discuss in their report about the biocompatibility of this new *** this work,the authors manifest that the quintessential graphene electrode and polymer blend of semiconducting single-wall carbon nanotube and C60 fullerene(S-SWCNT:C60)sustains its optoelectronic properties all through the various strides for neural ***,the studies show that these materials can provide a favourable environment for cell proliferation and a high degree of ***-12 cells used as a valuable model to validate the biocompatibility were grown successfully onto the S-SWCNT:C60 active layer still preserving its optical and electrical *** improved electrical performance of nano photodiode made of graphene,S-SWCNT:C60 established in the previous studies and the excellent bio-interface performance of this nano photodiode shows that the nano photodiode array proposed by the authors is a strong candidate for subretinal implants.

关键词： interface optoelectronic neural

Floral resources, pollinators and fruiting in a threatened tropical deciduous tree

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Journal of Plant Ecology 2011年第4期4卷 259-267页

作者： Manju V.Sharma R.Uma Shaanker Simon R.Leather R.Vasudeva Kundaranahalli R.Shivanna Ashoka Trust for Research in Ecology and the Environment Royal EnclaveSrirampuraJakkur POBangalore 560064India Division of Biology Imperial College LondonSilwood Park CampusAscotBerkshire SL57PYUK department of Crop Physiology University of Agricultural SciencesGKVKBangalore 560065India department of Forest Biology College of ForestrySirsiUttara Kannada DistrictIndia

Nothapodytes nimmoniana(Family Icacinaceae)is a deciduous tree species distributed in Asia facing severe population *** chips from the tree are a source of camptothecin,a globally soughtafter alkaloid with cancer-treating properties,and are harvested unsustainably in natural *** studied the pollination ecology of the species and asked if there are constraints in pollination and fruiting success in its natural *** also discuss the potential effects of wood extraction on pollinators and reproductive success in the *** The study was carried out during the flowering season in two populations with varying exploitation levels,both located in the Protected Area Network in the Western Ghats of Karnataka *** assessed floral resource availability and measured pollinator diversity and activity in the canopy from the perspective of the biology of the *** quantified pollinator abundance and percent fruit set as a function of population *** Findings Flowers belong to the fly pollination syndrome and are open to visits by generalists such as Apis dorsata and Trigona *** success did not vary between exploited and unexploited sites,but there was a significant difference in pollinator *** a lean male flowering year,fruit set was low,suggesting pollen limitation in the *** fruiting was recorded in the second year at the site where trees were felled soon after the flowering *** argue that when male floral resources are altered in exploited populations,pollinators of generalist nature may show a shift in foraging ***,generalist plants may be as susceptible to pollination loss as are specialist *** need for outcrossing in the species would probably exacerbate this vulnerability.

关键词： fruit set generalist over-harvest pollen limitation pollinator assemblage

Revisiting the role of mesenchymal stem cells in tuberculosis and other infectious diseases

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Cellular & Molecular Immunology 2023年第6期20卷 600-612页

作者： Annu Devi Isha Pahuja Shashi Prakash Singh Akanksha Verma Debapriya Bhattacharya Ashima Bhaskar Ved Prakash Dwivedi Gobardhan Das Special Centre for Molecular Medicine Jawaharlal Nehru UniversityNew DelhiIndia Immunobiology Group International Centre for Genetic Engineering and Biotechnology New DelhiIndia department of Molecular Medicine Jamia Hamdard UniversityNew DelhiIndia Centre for biotechnology Siksha O Anusandhan UniversityBhubneshwarOdishaIndia

Mesenchymal stem cells(MsCs)play diverse roles ranging from regeneration and wound healing to immune *** investigations have indicated the crucial role of these multipotent stem cells in regulating various aspects of the immune *** express unique signaling molecules and secrete various soluble factors that play critical roles in modulating and shaping immune responses,and in some other cases,MSCs can also exert direct antimicrobial effects,thereby helping with the eradication of invading ***,it has been demonstrated that MSCs are recruited at the periphery of the granuloma containing Mycobacterium tuberculosis and exert"Janus"-like functions by harboring pathogens and mediating host protective immune *** leads to the establishment of a dynamic balance between the host and the *** function through various immunomodulatory factors such as nitric oxide(NO),IDO,and immunosuppressive ***,our group has shown that *** uses MSCs as a niche to evade host protective immune surveillance mechanisms and establish *** also express a large number of ABC efflux pumps;therefore,dormant *** residing in MSCs are exposed to a suboptimal dose of ***,it is highly likely that drug resistance is coupled with dormancy and originates within *** this review,we discussed various immunomodulatory properties of MsCs,their interactions with important immune cells,and soluble *** also discussed the possible roles of MsCs in the outcome of multiple infections and in shaping the immune system,which may provide insight into therapeutic approaches using these cells in different infection models.

关键词： Mesenchymal Stem Cells(MsCs) Macrophages Tuberculosis Infectious Diseases T cells Immunomodulation Immunotherapy.

Effect of heavy metals and phenol on bacterial decolourisation and COD reduction of sucrose-aspartic acid Maillard product

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Journal of Environmental Sciences 2013年第1期25卷 172-180页

作者： Sangeeta Yadav Ram Chandra department of Environmental Microbiology School for Environmental SciencesBabasaheb Bhimrao Ambedkar University (A Central University)

Melanodins are amino-carbonyl complex, predominantly present in sugarcane molasses based distillery wastewater as major source of colourant. The microbial decolourisafion of melanoidin is a challenge due to its binding property with other co-pollutants of distillery waste. Results revealed that the presence of Zn2＋（2.00-20.00 mg/L） in melanoidin solution （1200 mg/L） stimulated the bacterial growth and sucrose-aspartic acid Maillard product （SAA） decolourisation as compared to control, while Fe3＋ and Mn2＋ at the same concentration inhibited the process. However, the presence of phenol （100 mg/L） along with Zn2＋, Fe3＋ and Mn2＋ suppressed the bacterial growth, SAA decolourisation and MnP activity. The shrinkage and reduced number of bacterial cell count at higher concentration of heavy metals in presence of phenol was also observed under scanning electron microscope.

关键词： degradation manganese peroxidase melanoidin