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Chinese Chemical Letters 2025年第2期36卷 378-383页

作者： Gangsheng Li Xiang Yuan Fu Liu Zhihua Liu Xujie Wang Yuanyuan Liu Yanmin Chen Tingting Wang Yanan Yang Peicheng Zhang State Key Laboratory of Bioactive Substance and Function of Natural Medicines Institute of Materia MedicaChinese Academy of Medical Sciences and Peking Union Medical College Beijing Wehand-Bio Pharmaceutical Co. Ltd.

The first synthesis of flavanostilbenes with a 2-cyclohepten-1-one core was carried out by applying an effective strategy in three steps from abundant polymerized flavanol resources. A key regio-and stereoselective Cu-mediated [5+2] cycloaddition/decarboxylation cascade was explored and applied without the use of protecting groups, and water as an environmentally friendly solvent contributed to the cascade. The intramolecular [5+2] cycloaddition mechanism, involving oxidation and dearomatization of the flavanol unit as a diene, was proposed and supported by the synthesis of the intermediate. The regioselectivity of the cyclization was found to be dependent on the substitution effects of the stilbene units by the exploration of substrate scope.

关键词： Biomimetic synthesis Copper Natural products Substrate [5 + 2] Cycloaddition

Full biosynthetic pathway of pyrrolobenzoxazines

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Chinese Chemical Letters 2025年第1期36卷 433-436页

作者： Kunya Wang Bingyu Liu Daojiang Yan Jian Bai Haibo Yu Youcai Hu State Key Laboratory of Bioactive Substance and Function of Natural Medicines Institute of Materia MedicaChinese Academy of Medical Sciences&Peking Union Medical CollegeBeijing 100050China

Pyrrolobenzoxazines are a rare terpene-amino acid family of natural products with potent biological ***,we reported the full biosynthetic pathway of paeciloxazine(1),a typical pyrrolobenzoxazine,with significant insecticidal *** on heterologous expression,chemical complement experiment,and in vitro biochemical assays,we demonstrated the sesquiterpene portion of 1 derived from discontinuously oxidations of amorphdiene,in which P450 monooxygenase PaxH catalyzed a cascade of hydroxylation and epoxidation,while two flavin dependent monooxygenases are involved in the transformation of the esterified tryptophan into a pyrrolobenzoxazine ***,a total of 15 compounds were generated through heterologous expression,of which 13,17 and 20 showed potential antiepileptic *** study fully elucidated the biosynthetic pathway of paeciloxazine(1)and showed the diversity and complexity of constructing natural products by organisms.

关键词： Pyrrolobenzoxazines Paeciloxazine Biosynthesis P450 monooxygenase Antiepileptic activity

Intratumoral injection of two dosage forms of paclitaxel nanoparticles combined with photothermal therapy for breast cancer

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Chinese Herbal Medicines 2025年第1期17卷 156-165页

作者： Lina Sun Cuiling Zuo Baonan Ma Xinxin Liu Yifei Guo Xiangtao Wang Meihua Han Institute of Medicinal Plant Development Chinese Academy of Medical Sciences & Peking Union Medical College Research Center of Pharmaceutical Engineering Technology Harbin University of Commerce

Objective:In order to enhance the efficacy of anti-breast cancer,paclitaxel nanoparticles（PTX NPs） and polypyrrole nanoparticles（PPy NPs） were combined with photothermal therapy and *** the same time,the two dosage forms of PTX NPs and PTX NPs gel were ***:PTX NPs were prepared by self-assembly method,and then the cytotoxicity in vitro was investigated by Methyl thiazolyl tetrazolium（MTT） and other methods,and the efficacy and side effects in vivo were further ***:The average hydrated diameter,PDI and electric potential of PTX NPs were（210.20±1.57） nm,（0.081±0.003） mV and（15.80±0.35） mV,*** results showed that the IC50value of PTX NPs on 4 T1 cells was 0.490 μg/mL,while that of PTX injection was 1.737 μg/*** cell inhibitory effect of PTX NPs was about 3.5 times higher than that of PTX *** tumor inhibition rates of PTX NPs and gel were 48.64% and 56.79%,*** with local photothermal stimulation,the tumor inhibition rate of the PTX NPs reached 91.05%,surpassing that of the gel under the same conditions（48.98%）,moreover,the organ index and H&E staining results of PTX NPs showed a decrease in ***:This combination therapy can significantly enhance the effect of anti-breast cancer,and the synergistic effect of chemotherapy and light and heat provides a feasible and effective strategy for the treatment of tumor.

关键词： breast cancer combination therapy paclitaxel photothermal therapy polypyrrole

The transcriptome of MHV-infected RAW264.7 cells offers an alternative model for macrophage innate immunity research

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Animal Models and Experimental Medicine 2025年第1期8卷 57-66页

作者： Yun Liu Ting-Ting Feng Wei Tong Zhi Guo Xia Li Qi Kong Zhi-Guang Xiang Institute of Laboratory Animal sciences Chinese Academy of Medical Sciences(CAMS)and Comparative Medicine CenterPeking Union Medical College(PUMC)BeijingP.R.China

Background:Macrophages are the primary innate immune cells encountered by the invading coronaviruses,and their abilities to initiate inflammatory reactions,to main-tain the immunity homeostasis by differential polarization,to train the innate immune system by epigenic modification have been reported in laboratory animal ***:In the current in vitro research,murine macrophage RAW 264.7 cell were infected by mouse hepatitis virus,a coronavirus existed in *** 3-,6-,12-,24-,and 48-h post infection(hpi.),the attached cells were washed with PBS and harvested in Trizol *** The harvest is subjected to transcriptome ***:The transcriptome analysis showed the immediate(3 hpi.)up regulation of DEGs related to inflammation,like Il1b and *** related to M2 differential po-larization,like Irf4 showed up regulation at 24 hpi.,the late term after viral *** addition,DEGs related to metabolism and histone modification,like Ezh2 were de-tected,which might correlate with the trained immunity of ***:The current in vitro viral infection study showed the key innated im-munity character of macrophages,which suggested the replacement value of viral infection cells model,to reduce the animal usage in preclinical research.

关键词： coronavirus innate immunity macrophage transcriptome

Particular Chinese contributions to extracorporeal liver surgery

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Hepatobiliary & Pancreatic Diseases International 2025年第1期24卷 57-66页

作者： Abudusalamu Aini Qian Lu Hao Wen Wen-Tao Wang Tuerganaili Aji Zhi-Yu Chen Lei-Da Zhang Zhan-Yu Yang Jia-Yin Yang Hai-Ning Fan Wei-Lin Wang Xiang-Cheng Li Yu Zhang Jia-Hong Dong Hepatopancreatobiliary Center Organ Transplantation CenterBeijing Tsinghua Changgung HospitalTsinghua UniversityBeijing 102218China Key Laboratory of Digital Intelligence Hepatology(Chinese Ministry of Education) School of Clinical MedicineTsinghua UniversityBeijing 100084China Research Unit of Precision Hepatobiliary Surgery Paradigm Chinese Academy of Medical SciencesBeijing 100010China Institute for Organ Transplantation and Bionics Institute for Precision MedicineSchool of Clinical MedicineTsinghua UniversityBeijing 100010China Hepatobiliary&Echinococcosis Surgery Digestive&Vascular Surgery CenterThe First Affiliated Hospital of Xinjiang Medical UniversityUrumqi 830054China Department of Liver Surgery&Liver Transplantation Center West China Hospital of Sichuan UniversityChengdu 610041China Department of Hepatobiliary Surgery Southwest HospitalThe Army Medical UniversityChongqing 100089China Faculty of Hepato-Pancreato-Biliary Surgery Chinese People’s Army General HospitalBeijing 400038China Liver Transplantation Center&Organ Transplantation Center West China Hospital of Sichuan UniversityChengdu 610041China Hepatopancreatobiliary Surgery School of Clinical MedicineQinghai University Affiliated HospitalXining 810006China Department of Hepatobiliary Surgery The Second Affiliated HospitalZhejiang Univeristy School of MedicineHangzhou 310009China Department of Hepatobiliary Surgery The First Affiliated Hospital of Nanjing Medical UniversityNanjing 210029China Department of Hepatobiliary Surgery Sichuan Provincial People’s HospitalChengdu 610072China

Extracorporeal liver surgery(ELS), also known as liver autotransplantation, is a hybrid(cross-fertilized) surgery incorporating the technical knowledge from extreme liver and transplant liver surgeries, and recently became more embraced and popularized among leading centers. ELS could be summarized into three major categories, namely, ex-situ liver resection and autotransplantation(ELRA), ante-situm liver resection and autotransplantation(ALRA) and auxiliary partial liver autotransplantation(APLA). The successful development of ELS during the past 37 years is definitely inseparable from continuous effort s done by Chinese surgeons and researchers. Especially, the precision liver surgery paradigm has allowed to transform ELS into a modularized, more simplified, and standardized surgery, to upgrade surgical skills, to improve peri-operative outcome and long-term survival, to increase the capability of surgeons to select more complex diseases and to expand the level of medical service to the population. This review highlights the Chinese contributions to the field of ELS, focusing thereby on features of different surgical types, technical innovations, disease selection and surgical indication, patient prognosis and future perspectives.

关键词： Ex-situ ex-vivo liver resection Ex-situ in-vivo liver resection Semi-ex-vivo liver resection Ex-situ liver resection after in-situ hepatectomy Liver autotransplantation Autologous liver transplantation Bench hepatectomy Back-table liver resection Precision liver surgery

Longitudinal landscape of immune reconstitution after acute SARS-CoV-2 infection at single-cell resolution

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Science Bulletin 2025年第3期70卷 323-327页

作者： Lili Ren Yiwei Liu Yeming Wang Chao Wu Li Guo Lan Chen Xinming Wang Yan Xiao Lixue Huang Jingchuan Zhong Jiacheng Yao Lu Liu Hui Li Ying Wang Yongchao Ma Yichunzi Zhang Lin Di Tao Dong Julian Knight Jianbin Wang Yanyi Huang Bin Cao Xianwen Ren Jianwei Wang National Health Commission Key Laboratory of Systems Biology of Pathogens and Christophe Mérieux Laboratory National Institute of Pathogen BiologyChinese Academy of Medical Sciences&Peking Union Medical CollegeBeijing 102629China Key Laboratory of Respiratory Disease Pathogenomics Chinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100730China State Key Laboratory of Respiratory Health and Multimorbidity Chinese Academy of Medical Sciences&Peking Union Medical CollegeBeijing 100029China Department of Pulmonary and Critical Care Medicine Institute of Respiratory Medicine of Chinese Academy of Medical ScienceNational Center for Respiratory MedicineCenter of Respiratory MedicineNational Clinical Research Center for Respiratory DiseasesChina-Japan Friendship HospitalBeijing 100029China College of Chemistry and Molecular Engineering Peking UniversityBeijing 100871China Biomedical Pioneering innovation Center(BIOPIC) Beijing Advanced Innovation Center for Genomics(ICG)College of Chemistryand Peking-Tsinghua Center for Life SciencesPeking UniversityBeijing 100871China MRC Human Immunology Unit MRC Weatherall Institute of Molecular MedicineRadcliffe Department of MedicineThe University of OxfordOxford OX39DSUK Chinese Academy of medical Science(cams)Oxford Institute(COI) University of OxfordOxford OX39DSUK Wellcome Centre for Human Genetics University of OxfordOxford OX39DSUK School of Life sciences Tsinghua-Peking Center for Life SciencesTsinghua UniversityBeijing 100084China Beijing Advanced innovation Center for Structural Biology(ICSB) Tsinghua UniversityBeijing 100084China Chinese Institute for Brain Research(CIBR) Beijing 102206China Institute for Cell Analysis Shenzhen Bay LaboratoryShenzhen 518107China Tsinghua University-Peking University Joint Center for Life sciences Beijing 100084China Biomedical Pioneering innovation Center(BIOPIC) Peking UniversityBeijing 100871China Changping Laboratory Beijing 102206China

The long-term consequences of severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)infection,known as the post-acute sequelae of COVID-19(PASC),have been a growing concern.A significant proportion of COVID-19 patients experience persistent health issues even six to twelve months after recovering from acute infection[1],[2],[3],[4].Therefore,understanding the immune reconstitution process and identifying the molecular and cellular mechanisms underlying PASC is crucial.

关键词： landscape consequences acute

Biomaterial‑based mechanical regulation facilitates scarless wound healing with functional skin appendage regeneration

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Military medical Research 2025年第1期12卷 96-120页

作者： Ying-Ying Li Shuai-Fei Ji Xiao-Bing Fu Yu-Feng Jiang Xiao-Yan Sun Research Center for Tissue Repair and Regeneration Affiliated to the medical innovation Research Department Chinese PLA General Hospital and PLA Medical CollegePLA Key Laboratory of Tissue Repair and Regenerative Medicine and Beijing Key Research Laboratory of Skin InjuryRepair and RegenerationResearch Unit of Trauma CareTissue Repair and RegenerationChinese Academy of Medical Sciences2019RU051Beijing 100048China Research Unit of Trauma Care Tissue Repair and RegenerationChinese Academy of Medical Sciences2019RU051Beijing 100048China 2Department of Tissue Regeneration and Wound RepairChinese PLA General HospitalBeijing 100853China

Scar formation resulting from burns or severe trauma can significantly compromise the structural integrity of skin and lead to permanent loss of skin appendages,ultimately impairing its normal physiological *** evidence underscores the potential of targeted modulation of mechanical cues to enhance skin regeneration,promoting scarless repair by influencing the extracellular microenvironment and driving the phenotypic *** field of skin repair and skin appendage regeneration has witnessed remarkable advancements in the utilization of biomaterials with distinct physical ***,a comprehensive understanding of the underlying mechanisms remains somewhat elusive,limiting the broader application of these *** this review,we present two promising biomaterial-based mechanical approaches aimed at bolstering the regenerative capacity of compromised *** first approach involves leveraging biomaterials with specific biophysical properties to create an optimal scarless environment that supports cellular activities essential for *** second approach centers on harnessing mechanical forces exerted by biomaterials to enhance cellular plasticity,facilitating efficient cellular reprogramming and,consequently,promoting the regeneration of skin *** summary,the manipulation of mechanical cues using biomaterial-based strategies holds significant promise as a supplementary approach for achieving scarless wound healing,coupled with the restoration of multiple skin appendage functions.

关键词： Scarless Wound healing Biomaterials Mechanical cues Skin appendages

DTD1 modulates synaptic efficacy by maintaining D-serine and D-aspartate homeostasis

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Science China(Life sciences) 2025年第2期68卷 467-483页

作者： Xiao Liu Chaojuan Yang Zhuoran Lin Jianing Li Bin Yin Xuepei Lei Wei Han Boqin Qiang Pengcheng Shu Chen Zhang Xiaozhong Peng State KeyLaboratoryofCommon Mechanism Research for Major Diseases Departmentof Biochemistry&Molecular BiologyMedicalPrimate Research CenterNeuroscienceCenterInstituteof Basic Medical Sciences Chinese Academy of Medical SciencesSchool of Basic Medicine Peking Union Medical CollegeBeijing 100005China State Key Laboratory of Respiratory Health and Multimorbidity Beijing 100005China Institute of Laboratory Animal sciences Chinese Academy of Medical Sciences&Peking Union Medical CollegeBeijing 100021China School of Basic medical sciences Beijing Key Laboratory of Neural Regeneration and RepairCapital Medical UniversityBeijing 100069China Key Laboratoryof Biomechanics and Mechanobiology(Beihang University) Ministryof EducationBeijing AdvancedInnovation Center for Biomedical EngineeringSchoolof Engineering MedicineBeihang UniversityBeijing 100191China Chinese Institute for Brain Research Beijing 102206China

D-serine and D-aspartate are involved in N-methyl-D-aspartate receptor(NMDAR)-related physiological and pathological ***-t RNA deacylase 1(DTD1)may biochemically contribute to D-serine or D-aspartate ***,it is unclear thus far whether DTD1 regulates D-serine or D-aspartate content in neurobiological *** the present research,we found that DTD1 was essential to maintain the D-serine or D-aspartate homeostasis,which was consistent with the phenomenon that DTD1-deficiency resulted in changes in the quantity changes of functional NMDAR subunits in postsynaptic ***,DTD1 played a considerable role in regulating dendritic morphology and synaptic *** a consequence,DTD1 affected neurobiological events,including the synaptic strength of the CA3-to-CA1 circuit,dendritic spine density of hippocampal pyramidal neurons,and behavioral performance of mice in the Morris water *** findings highlight the important role of DTD1 in synaptic transmission,neuronal morphology,and spatial learning and memory and suggest an undisclosed mechanism of DTD1 that participates the regulation of D-serine or D-aspartate homeostasis in hippocampal neurons.

关键词： D-aminoacyl-tRNA deacylase 1 D-serine D-aspartate synaptic transmission neuronal morphology spatial learning and memory

A Case Study on Garbage Code Redistribution Methods for Heart Failure at City Level by Two Approaches

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Biomedical and Environmental sciences 2025年第1期38卷 119-125页

作者： Liqun Liu Zemin Cai Xuewei Wang Chunping Wang Xiangyun Ma Xianfeng Meng Bofu Ning Ning Li Xia Wan Institute of Basic medical sciences Chinese Academy of Medical Sciences(CAMS)and School of Basic MedicinePeking Union Medical College(PUMC)Beijing 100005China State Key Laboratory of Respiratory Health and Multimorbidity Beijing 100005China School of Public Health Weifang Medical UniversityWeifang 261042ShandongChina Xuanwei Center for Disease Control and Prevention Xuanwei 655400YunnanChina Weifang Center for Disease Control and Prevention Weifang 261061ShandongChina

Cause of death surveillance data is most important for developing effective health policies,whose quality is crucially affected by the accuracy of the underlying cause of death(UCOD)provided in death *** World Health Organization(WHO)has clearly defined a UCOD as“the disease or injury which initiated the train of morbid events leading directly to death,or the circumstance of the accident or violence which produced the fatal injuries”[1].

关键词： injuries initiated distribution

Antithetical impacts of deleterious LRP1B mutations in non-squamous and squamous NSCLCs on predicting benefits from immune checkpoint inhibitor alone or with chemotherapy over chemotherapy alone: retrospective analyses of the POPLAR/ OAK and CHOICE-01 trials

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Science China(Life sciences) 2025年第1期68卷 249-262页

作者： Jinliang Wang Wenyong Zhou Yu Xu Jianchun Duan Qiaoxia Zhou Guoqiang Wang Leo Li Chunwei Xu Wenxian Wang Shangli Cai Zhijie Wang Jie Wang Department of Oncology The Fifth Medical Center of Chinese PLA General HospitalBeijing 100091China Department of Thoracic Surgery Shanghai Chest HospitalSchool of MedicineShanghai Jiao Tong UniversityShanghai 200025China Burning Rock Biotech Guangzhou 510300China cams Key Laboratoryof Translational Research on LungCancer State Key Laboratoryof Molecular OncologyDepartmentof Medical OncologyNational Cancer Center/National Clinical Research Center for Cancer/Cancer HospitalChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100021China Institute of Basic Medicine and Cancer(IBMC) Chinese Academy of SciencesHangzhou 310000China Department of Clinical Trial The Cancer Hospital of the University of Chinese Academy of Sciences(Zhejiang Cancer Hospital)Hangzhou 310022China

In non-small cell lung cancers,the non-squamous and squamous subtypes(nsqNSCLC and sqNSCLC)exhibit disparities in pathophysiology,tumor immunology,and potential genomic correlates affecting responses to immune checkpoint inhibitor(ICI)-based *** our inhouse training cohort(n=85),the presence of the LRP1B deleterious mutation(LRP1B-del)was associated with longer and shorter progression-free survival(PFS)on ICIs alone in nsqNSCLCs and sqNSCLCs,respectively(Pinteraction=0.008).These results were validated using a larger public ICI cohort(n=208,Pinteraction<0.001).Multiplex immunofluorescence staining revealed an association between LRP1B-del and increased and decreased numbers of tumor-infiltrating CD8+T cells in nsqNSCLCs(P=0.040)and sqNSCLCs(P=0.014),*** the POPLAR/OAK cohort,nsqNSCLCs with LRP1B-del demonstrated improved PFS benefits from atezolizumab over docetaxel(hazard ratio(HR)=0.70,P=0.046),whereas this benefit was negligible in those without LRP1B-del(HR=1.05,P=0.64).Conversely,sqNSCLCs without LRP1Bdel benefited more from atezolizumab(HR=0.60,P=0.002)than those with LRP1B-del(HR=1.30,P=0.31).Consistent results were observed in the in-house CHOICE-01 cohort,in which nsqNSCLCs with LRP1B-del and sqNSCLCs without LRP1B-del benefited more from toripalimab plus chemotherapy than from chemotherapy alone(Pinteraction=0.008).This multi-cohort study delineates the antithetical impacts of LRP1Bdel in nsqNSCLCs and sqNSCLCs on predicting the benefits from ICI alone or with chemotherapy over chemotherapy *** findings highlight the distinct clinical utility of LRP1B-del in guiding treatment choices for nsqNSCLCs and sqNSCLCs,emphasizing the necessity for a detailed analysis based on pathological subtypes when investigating biomarkers for cancer therapeutics.

关键词： non-squamous NSCLC squamous NSCLC LRP1B deleterious mutations predictive biomarker immune checkpoint inhibitor