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Spatial and diel variations of the prokaryotic community in the Phaeocystis globosa blooms area of Beibu Gulf,china

Acta Oceanologica Sinica 2022年第12期41卷 87-97页

作者： Cheng He Sha Xu Shuqun Song Caiwen Li North china Sea Environmental Monitoring Center State Oceanic AdministrationQingdao 266033China Key Laboratory of Ecological Prewarning Protection and Restoration of Bohai SeaMinistry of Natural ResourcesQingdao 266033China Key Laboratory of Marine Ecology and Environmental Sciences Chinese Academy of SciencesQingdao 266071China Marine Ecology and Environmental Science Laboratory Pilot National Laboratory for Marine Science and Technology(Qingdao)Qingdao 266237China Center for Ocean Mega-Science Chinese Academy of SciencesQingdao 266071China Shaanxi Provincial Academy of Environmental Science Xi’an 710061China

While prokaryotes play key roles in nutrient cycling and energy flow during Phaeocystis globosa blooms,the information on the spatial and diel temporal distribution of the prokaryotic community during Phaeocystis blooms remains *** January 2019,we used high-throughput sequencing of the 16S rRNA gene to explore the spatial and diel variations of particle-attached(PA)and free-living(FL)prokaryotic communities during the blooming phase of *** in Beibu Gulf,Guangxi,*** results suggested a significant spatial variation pattern in the horizontal distribution of prokaryotic communities,while there was no significant difference in the vertical *** spatial distance and environmental variables shaped the horizontal distribution of the prokaryotic community structure,while environmental variables,particularly the abundance of *** colony and Chl a,showed more significant influence and were closely related to the structure and variation of the prokaryotic *** vertical mixing of the water column disrupted the vertical structure heterogeneity of the prokaryotic community in *** were significant differences in the diel samples of PA prokaryotic communities,but not in the FL prokaryotic ***,ammonium and the abundance of *** colony were the key environmental variables impacting the diel variations of prokaryotic communities over the sampling *** present study provided valuable information to depict the spatial-temporal variations of the microbial community and its association with environmental parameters during *** bloom in the tropical gulf.

关键词： algal bloom 16S rRNA genes prokaryotic community diversity spatial patterns diel distribution

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Improving the genome and proteome annotations of the marine model diatom Thalassiosira pseudonana using a proteogenomics strategy

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Marine Life Science & Technology 2023年第1期5卷 102-115页

作者： Xiao-Huang Chen Ming-Kun Yang Yuan-Yuan Li Zhang-Xian Xie Shu-Feng Zhang Mats Töpel Shady A.Amin Lin Lin Feng Ge Da-Zhi Wang State Key Laboratory of Marine Environmental Science/College of the Environment and Ecology Xiamen UniversityXiamen361005China Southern Marine Science and Engineering Guangdong Laboratory(Zhuhai) Sun Yat-Sen UniversityZhuhai519082China Key Laboratory of Algal Biology Institute of HydrobiologyChinese Academy of SciencesWuhan430072China College of Chemistry Chemical Engineering and EnvironmentMinnan Normal UniversityZhangzhou363000China Department of Marine Sciences University of GothenburgBox 46140530GothenburgSweden IVL-Swedish Environmental Research Institute Box 5302140014GothenburgSweden New York University Abu Dhabi Saadiyat Island129188Abu DhabiUnited Arab Emirates

Diatoms are unicellular eukaryotic phytoplankton that account for approximately 20%of global carbon fixation and 40%of marine primary productivity;thus,they are essential for global carbon biogeochemical cycling and *** availability of ten diatom genome sequences has facilitated evolutionary,biological and ecological research over the past decade;however,a complimentary map of the diatom proteome with direct measurements of proteins and peptides is still ***,we present a proteome map of the model marine diatom Thalassiosira pseudonana using high-resolution mass spectrometry combined with a proteogenomic ***-depth proteomic profiling of three different growth phases and three nutrient-deficient samples identified 9526 proteins,accounting for~81%of the predicted protein-coding *** analysis identified 1235 novel genes,975 revised genes,104 splice variants and 234 single amino acid ***,our quantitative proteomic analysis experimentally demonstrated that a considerable number of novel genes were differentially translated under different nutrient *** findings substantially improve the genome annotation of *** and provide insights into new biological functions of *** relatively comprehensive diatom proteome catalog will complement available diatom genome and transcriptome data to advance biological and ecological research of marine diatoms.

关键词： Marine diatoms Thalassiosira pseudonana Proteome Proteogenomics

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Comparison of time-restricted and ad libitum self-feeding on the growth,feeding behavior and daily digestive enzyme profiles of Atlantic salmon

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Chinese Journal of Oceanology and Limnology 2017年第4期35卷 729-736页

作者：史策刘鹰衣萌萌郑纪盟田会芹杜以帅李贤孙国祥 Institute of Oceanology Chinese Academy of Sciences National & Local Joint Engineering Laboratory of Ecological Mariculture Dalian Ocean University School of Marine Science and Environment Engineering University of Chinese Academy of Sciences

Although it has been hypothesized that a predictable feeding regime in animals allows physiological variables to be adjusted to maximize nutrient utilization and,hence,better growth performance,the assumption has rarely been *** study compares the effects of time-restricted versus free access self-feeding on the growth,feeding behavior and daily digestive enzyme rhythms of Atlantic salmon(Salmo salar).In an experiment that lasted 6 weeks,fish(109.9 g) were divided into two groups:group 1 had free access to a self-feeder(FA);group 2 received three meals per day(2 h per meal) at dawn,midday and dusk via a time-restricted self-feeder(TR).At the end of the experiment,the fish were sampled every 3 h over a24-h *** results showed that the TR fish quickly synchronized their feeding behavior to the feeding window and their blood glucose showed a significant postprandial increase,while FA fish displayed no statistically significant rhythms(P>0.05).Pepsin activity of TR fish also showed a significant daily rhythm(P0.05).In conclusion,the study failed to confirm a link between the entrainment of daily digestive enzyme profiles and growth performance,with the TR group showing comparatively poor blood glucose regulation.

关键词： Atlantic salmon (Salmo salar) self-feeding regime feeding behavior digestive physiology daily rhythm

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Advanced Biotechnology for Cell Cryopreservation

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Transactions of Tianjin University 2020年第6期26卷 409-423页

作者： Jing Yang Lei Gao Min Liu Xiaojie Sui Yingnan Zhu Chiyu Wen Lei Zhang Department of Biochemical Engineering School of Chemical Engineering and TechnologyTianjin UniversityTianjin 300350China Frontier Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering(MOE) School of Chemical Engineering and TechnologyTianjin UniversityTianjin 300350China qingdao Institute for Marine Technology of Tianjin University Qingdao 266235China

Cell cryopreservation has evolved as an important technology required for supporting various cell-based applications,such as stem cell therapy,tissue engineering,and assisted *** times have witnessed an increase in the clinical demand of these applications,requiring urgent improvements in cell ***,cryopreservation technology suff ers from the issues of low cryopreservation effi ciency and cryoprotectant(CPA)*** of advanced biotechnology tools can signifi cantly improve post-thaw cell survival and reduce or even eliminate the use of organic solvent CPAs,thus promoting the development of ***,based on the diff erent cryopreservation mechanisms available,we provide an overview of the applications and achievements of various biotechnology tools used in cell cryopreservation,including trehalose delivery,hydrogel-based cell encapsulation technique,droplet-based cell printing,and nanowarming,and also discuss the associated challenges and perspectives for future development.

关键词： Cell cryopreservation Biotechnology Trehalose delivery Hydrogel-based cell encapsulation Droplet-based cell printing Nanowarming

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Rational construction and triethylamine sensing performance of foam shapedα-MoO_(3)@SnS_(2) nanosheets

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Chinese Chemical Letters 2022年第1期33卷 567-572页

作者： Xianhui Dong Qing Han Yaru Kang Haidong Li Xinyu Huang Zhengtao Fang Huimin Yuan Ahmed A.Elzatahry Zongtao Chi Guanglei Wu Wanfeng Xie School of Material Science&Engineering Institute of Materials for Energy and EnvironmentState Key Laboratory of Bio-Fibers and Eco-TextilesQingdao UniversityQingdao 266071China School of Electronics and Information Qingdao UniversityQingdao 266071China College of Physics and Electronic Engineering Qilu Normal UniversityJi'nan 250200China Materials Science and Technology program College of Arts and SciencesQatar UniversityPO Box 2713DohaQatar

Owing to their high surface area,stable structure and easy fabrication,composite nanomaterials with encapsulation structures have attracted considerable research interest as sensing materials to detect volatile organic ***,a hydrothermal route is designed to prepare foam shapedα-MoO_(3)@SnS_(2)nanosheets that exhibit excellent sensing performance for triethylamine(TEA).The developed sensor,based onα-MoO_(3)@SnS_(2)nanosheets,displays a high response of 114.9 for 100 ppm TEA at a low working temperature of 175℃with sensitivity higher than many other reported *** addition,the device shows a wide concentration detection range(from 500 ppb to 500 ppm),good stability after exposure to air for 80 days,and excellent *** superior sensing characteristics of the developed sensor are attributed to the high crystallinity ofα-MoO_(3)/SnS_(2),excessive and accessible active sites provided by the good permeability of porous SnS_(2)shells,and the excellent conductivity of the encapsulation heterojunction ***,the foam shapedα-MoO_(3)@SnS_(2)nanosheets presented herein have promising practical applications in TEA gas sensing devices.

关键词： MoO_(3) SnS_(2) Encapsulation heterojunction Triethylamine Sensing performance

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Quantitative proteomics provides insight into the response of the marine dinoflagellate Prorocentrum donghaiense to changes in ambient phosphorus

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Journal of Oceanology and Limnology 2022年第2期40卷 563-576页

作者： Shufeng ZHANG Chunjuan YUAN Ying CHEN Lin LIN Dazhi WANG State Key Laboratory of Marine Environmental Science/College of the Environment and Ecology Xiamen UniversityXiamen 361102China Key Laboratory of Marine Ecology&Environmental Sciences Institute of OceanologyChinese Academy of SciencesQingdao 266071China

Dinofl agellates are the major causative agents of harmful algal blooms in the global ocean and they usually form blooms under conditions of very low dissolved inorganic phosphorus(DIP).However,the mechanisms underpinning the dinofl agellate blooms remain ***,we quantitatively compared protein expression profi les of a marine dinofl agellate,Prorocentrum donghaiense,grown in inorganic P-replete,P-defi cient,and DIP-and dissolved organic phosphorus(DOP)-resupplied conditions by employing a Tandem Mass Tag(TMT)-based quantitative proteomic *** involved in intracellular P reallocation,organic P,and non-P lipid utilization were up-regulated under the P-defi cient condition,while inorganic phosphate transporters varied insignifi *** response to the P resupplementation,nitrogen metabolism,ribosome,porphyrin,and chlorophyll metabolism were up-regulated,while lysosome,and starch and sucrose metabolism were ***,photosynthesis was up-regulated and secondary metabolism was down-regulated only in the DIP-resupplied cells,whereas amino acid metabolism and vitamin B6 metabolism were up-regulated in the DOP-resupplied cells,indicating diff erential response mechanisms of *** to DIP or DOP *** results indicated that *** initiated multiple strategies in response to an ambient inorganic P-defi ciency,and its efficient DOP assimilation by providing both P and carbon sources might be a key factor driving bloom formations of *** in a low DIP environment.

关键词： marine dinofl agellates harmful algal blooms Prorocentrum donghaiense phosphorus quantitative proteomics

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Identification and functional prediction of long intergenic noncoding RNAs in fetal porcine longissimus dorsi muscle

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Journal of Integrative Agriculture 2021年第1期20卷 201-211页

作者： LI Cen-cen YU Shu-long REN Hai-feng WU Wei WANG Ya-ling HAN Qiu XU Hai-xia XU Yong-jie ZHANG Peng-peng College of Life Sciences Xinyang Normal UniversityXinyang 464000P.R.China

Pigs are globally farmed animals which provide protein for human consumption in the form of skeletal muscle. To better understand the function of long intergenic noncoding RNAs(linc RNAs) in porcine skeletal muscle growth and development, we collected RNA-seq data from porcine longissimus dorsi muscle(LDM) during embryonic development. We identified a total of 739 linc RNA transcripts, which were distributed on all chromosomes except the chromosome Y, and analyzed their molecular characteristics. Compared to protein-coding genes, linc RNAs showed shorter transcripts, longer exons, fewer exons and higher tissue specificity. In addition, the abundance of linc RNAs in five embryonic development stages were analyzed and 45 differentially expressed linc RNAs were screened, three of which were highly expressed in LDM during porcine embryonic development. Finally, we predicted the potential target genes and functions of the linc RNAs, and identified 1 537 cis-target genes and 8 571 trans-target genes. Furthermore, we identified two key candidate linc RNAs involved in muscle development, XLOC_024652 and XLOC_001832, for post-trial validation. Our results provide a genome-wide resource of linc RNAs which are potentially involved in porcine embryonic skeletal muscle development and lay a foundation for the further study of their functions.

关键词： lincRNAs pig skeletal muscle embryo potential target genes

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Recent Advances in Nano‑Enabled Seed Treatment Strategies for Sustainable Agriculture:Challenges,Risk Assessment,and Future Perspectives

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Nano-Micro Letters 2023年第4期15卷 153-189页

作者： Amruta Shelar Shivraj Hariram Nile Ajay Vikram Singh Dirk Rothenstein Joachim Bill Jianbo Xiao Manohar Chaskar Guoyin Kai Rajendra Patil Department of Technology Savitribai Phule Pune UniversityPuneMaharashtra 411007India Zhejiang Provincial International S&T Cooperation Base for Active Ingredients of Medicinal and Edible Plants and Health School of Pharmaceutical ScienceJinhua AcademyZhejiang Chinese Medical UniversityHangzhou 310053ZhejiangPeople’s Republic of China Department of Chemical and Product Safety German Federal Institute for Risk Assessment(BfR)Max‑Dohrn‑Strasse10589 BerlinGermany Institute for Materials Science University of Stuttgart70569 StuttgartGermany International Research Center for Food Nutrition and Safety Jiangsu UniversityZhenjiang 212013People’s Republic of China Faculty of Science and Technology Savitribai Phule Pune UniversityPuneMaharashtra 411007India Department of Biotechnology Savitribai Phule Pune UniversityPuneMaharashtra 411007India

Agro seeds are vulnerable to environmental stressors,adversely affecting seed vigor,crop growth,and crop *** agrochemical-based seed treatments enhance seed germination,but they can also cause damage to the environment;therefore,sustainable technologies such as nano-based agrochemicals are urgently *** can reduce the dose-dependent toxicity of seed treatment,thereby improving seed viability and ensuring the controlled release of nanoagrochemical active ingredients However,the applications of nanoagrochemicals to plants in the field raise concerns about nanomaterial safety,exposure levels,and toxicological implications to the environment and human *** the present comprehensive review,the development,scope,challenges,and risk assessments of nanoagrochemicals on seed treatment are ***,the implementation obstacles for nanoagrochemicals use in seed treatments,their commercialization potential,and the need for policy regulations to assess possible risks are also *** on our knowledge,this is the first time that we have presented legendary literature to readers in order to help them gain a deeper understanding of upcoming nanotechnologies that may enable the development of future generation seed treatment agrochemical formulations,their scope,and potential risks associated with seed treatment.

关键词： Agro seeds Environmental seed stressors Nanoagrochemicals Toxicological implications Risk regulations

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Synthesis of hierarchical nanohybrid CNT@Ni-PS and its applications in enhancing the tribological,curing and thermal properties of epoxy nanocomposites

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Frontiers of Chemical Science and Engineering 2021年第5期15卷 1281-1295页

作者： Jinian Yang Yuxuan Xu Chang Su Shibin Nie Zhenyu Li School of Materials Science and Engineering Anhui University of Science and TechnologyHuainan232001China School of Energy Resources and Safety Anhui University of Science and TechnologyHuainan232001China School of Mechanical Engineering Anhui University of Science and TechnologyHuainan232001China

Poor interfacial adhesion and dispersity severely obstruct the continued development of carbon nanotube(CNT)-reinforced epoxy(EP)for potential ***,hierarchical CNT nanohybrids using nickel phyllosilicate(Ni-PS)as surface decorations(CNT@Ni-PS)were synthesized,and the nanocomposites derived from varied mass fractions of EP and CNT@Ni-PS were *** morphological structures,tribological performances,curing behaviors and thermal properties of EP/CNT@Ni-PS nanocomposites were carefully *** show that hierarchical CNT nanohybrids with homogeneous dispersion and well-bonded interfacial adhesion in the matrix are successfully obtained,presenting significantly improved thermal and tribological ***,analysis on cure kinetics proves the excellent promotion of CNT@Ni-PS on the non-isothermal curing process,lowering the curing energy barrier steadily.

关键词： nickel phyllosilicate surface decoration tribological property curing kinetics thermal performance

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The brassinosteroid signaling component SlBZR1 promotes tomato fruit ripening and carotenoid accumulation

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Journal of Integrative Plant Biology 2023年第7期65卷 1794-1813页

作者： Fanliang Meng Haoran Liu Songshen Hu Chengguo Jia Min Zhang Songwen Li Yuanyuan Li Jiayao Lin Yue Jian Mengyu Wang Zhiyong Shao Yuanyu Mao Lihong Liu Qiaomei Wang Key Laboratory of Horticultural Plant Growth and Development Ministry of AgricultureDepartment of HorticultureZhejiang UniversityHangzhou 310058China College of Plant Science Jilin UniversityChangchun 130062China

The plant hormone ethylene is essential for climacteric fruit ripening, although it is unclear how other phytohormones and their interactions with ethylene might affect fruit ripening. Here, we explored how brassinosteroids(BRs) regulate fruit ripening in tomato(Solanum lycopersicum) and how they interact with ethylene. Exogenous BR treatment and increased endogenous BR contents in tomato plants overexpressing the BR biosynthetic gene SlCYP90B3promoted ethylene production and fruit *** analysis indicated that the BR signaling regulators Brassinazole-resistant1(SlBZR1) and BRI1-EMS-suppressor1(SlBES1) act redundantly in fruit softening. Knocking out Sl BZR1 inhibited ripening through transcriptome reprogramming at the onset of ripening. Combined transcriptome deep sequencing and chromatin immunoprecipitation followed by sequencing identified 73 Sl BZR1-repressed targets and 203 Sl BZR1-induced targets involving major ripening-related genes, suggesting that Sl BZR1 positively regulates tomato fruit *** BZR1 directly targeted several ethylene and carotenoid biosynthetic genes to contribute to the ethylene burst and carotenoid accumulation to ensure normal ripening and quality formation. Furthermore,knock-out of Brassinosteroid-insensitive2(Sl BIN2),a negative regulator of BR signaling upstream of Sl BZR1, promoted fruit ripening and carotenoid accumulation. Taken together, our results highlight the role of Sl BZR1 as a master regulator of tomato fruit ripening with potential for tomato quality improvement and carotenoid biofortification.

关键词： brassinosteroids carotenoids ethylene fruit ripening quality improvement SlBIN2 SlBZR1 tomato

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