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A nanobody-based blocking enzyme-linked immunosorbent assay for detecting antibodies against pseudorabies virus glycoprotein E

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Journal of Integrative Agriculture 2024年第4期23卷 1354-1368页

作者： Huanhuan Lü Pinpin Ji Siyu Liu Ziwei Zhang Lei Wang Yani Sun Baoyuan Liu Lizhen Wang Qin Zhao Department of Preventive Veterinary Medicine College of Veterinary MedicineNorthwest A&F University/Shaanxi Scientific Observing and Experimental Station of Veterinary Pharmacology and Diagnostic TechnologyMinistry of Agriculture and Rural AffairsYangling 712100China

Pseudorabies(PR)is an acute infectious disease of pigs caused by the PR virus(PRV)and results in great economic losses to the pig industry *** glycoprotein E(gE)-based enzyme-linked immunosorbent assay(ELISA)has been used to distinguish gE-deleted vaccine-immunized pigs from wild-type virus-infected pigs to eradicate PR in some *** has the advantages of small size and easy genetic engineering and has been a promising diagnostic ***,there were few reports about developing nanobody-based ELISA for detecting anti-PRV-gE *** the present study,the recombinant PRV-gE was expressed with a bacterial system and used to immunize the Bactrian ***,two nanobodies against PRV-gE were screened from the immunized camel by phage display ***,two nanobody-HRP fusion proteins were expressed with HEK293T *** PRV-gE-Nb36-HRP fusion protein was selected as the probe for developing the blocking ELISA(bELISA)to detect anti-PRV-gE *** optimizing the conditions of bELISA,the amount of coated antigen was 200 ng per well,and dilutions of the fusion protein and tested pig sera were separately 1:320 and 1:*** cut-off value of bELISA was 24.20%,and the sensitivity and specificity were 96.43 and 92.63%,*** detecting 233 clinical pig sera with the developed bELISA and a commercial kit,the results showed that the coincidence rate of two assays was 93.99%.Additionallly,epitope mapping showed that PRV-gE-Nb36 recognized a conserved conformational epitope in different reference PRV ***,great stability and low-cost nanobody-based bELISA for detecting anti-PRV-gE antibodies were *** bELISA could be used for monitoring and eradicating PR.

关键词： nanobody nanobody-HRP blocking ELISA PRV-gE antibody

采用两步炭化法和熔盐模板法制备N、S共掺杂煤基硬炭及共储钠性能（英文）

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新型炭材料(中英文) 2024年第2期39卷 297-307页

作者：牛慧祝王海花孙立宇杨晨榕王雨曹瑞杨存国王洁舒珂维陕西科技大学化学与化工学院陕西科技大学西安市先端性能材料与高分子创新团队陕西科技大学陕西省工业化学添加剂重点实验室西安北方惠安化学工业有限公司

硬炭因资源丰富、结构稳定及安全性高等优势，已成为钠离子电池常用阳极材料。其中，煤基衍生硬炭受到了广泛的关注。本工作以长焰煤为碳源，硫脲为氮硫源，NaCl为模板，通过两步炭化工艺和杂原子掺杂相结合的方法合成了N和S共掺杂的煤... 详细信息

硬炭因资源丰富、结构稳定及安全性高等优势，已成为钠离子电池常用阳极材料。其中，煤基衍生硬炭受到了广泛的关注。本工作以长焰煤为碳源，硫脲为氮硫源，NaCl为模板，通过两步炭化工艺和杂原子掺杂相结合的方法合成了N和S共掺杂的煤基硬炭（NSPC1200）。两步炭化过程在调节碳微晶结构和扩大层间距方面发挥了重要的作用。N和S的共掺杂调节了炭材料的电子结构，赋予其更多的活性位点；此外，引入NaCl作为模板有助于孔结构的构建，有利于电极和电解质之间的接触，从而实现Na+和电子的有效传输。在协同作用下，样品NSPC1200表现出优异的储钠能力，在20 mAg-1电流密度下呈现314.2 mAhg-1的可逆容量。即使在100 mAg-1下循环200次，仍保持224.4 mAhg-1的比容量。这项工作成功实现了策略性调整煤基炭材料微观结构的目标，最终获得了具有优异的电化学性能的硬炭阳极。

关键词：硬炭钠离子电池煤衍生炭两步炭化氮硫共掺杂

AtRKD5 inhibits the parthenogenic potential mediated by AtBBM

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Journal of Integrative Plant Biology 2024年第7期66卷 1517-1531页

作者： Qiyan Liu Dongfen Han Denghu Cheng Jinfan Chen Shujuan Tian Jiafa Wang Man Liu Li Yuan State key Laboratory of Crop Stress Resistance and High‐Efﬁciency Production College of HorticultureNorthwest A&F UniversityYangling 712100China

Parthenogenesis,the development of unfertilized egg cells into embryos,is a key component of ***(BABY BOOM),a crucial regulator of embryogenesis in Arabidopsis,possesses the capacity to shift nutritional growth toward reproductive ***,the mechanisms underlying AtBBM-induced parthenogenesis remain largely unexplored in dicot *** findings revealed that in order to uphold the order of sexual reproduction,the embryo-specific promoter activity of AtBBM as well as repressors that inhibit its expression in egg cells combine to limiting its ability to induce ***,AtRKD5,a RWPRK domain-containing(RKD)transcription factor,binds to the 3'end of AtBBM and is identified as one of the inhibitory factors for AtBBM expression in the egg *** the atrkd5 mutant,we successfully achieved enhanced ectopic expression of AtBBM in egg cells,resulting in the generation of haploid offspring via parthenogenesis at a rate of 0.28%.Furthermore,by introducing chimeric Arabidopsis and rice BBM genes into the egg cell,we achieved a significant 4.6-fold enhancement in haploid induction through the atdmp8/9 *** findings lay a strong foundation for further exploration of the BBM-mediated parthenogenesis mechanism and the improvement of haploid breeding efficiency mediated by the dmp8/9 mutant.

关键词： AtBBM atdmp AtRKD5 haploid induction parthenogenesis

Histological,transcriptomic,and gene functional analyses reveal the regulatory events underlying gibberellin-induced parthenocarpy in tomato

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Horticultural Plant Journal 2024年第1期10卷 156-170页

作者： Shaobo Niu Yu He Siwei Yan Zhengliang Sun Run Cai Yan Zhang shaanxi Engineering Research Center for Vegetables College of HorticultureNorthwest A&F UniversityYanglingShaanxi 712100China

Gibberellin(GA)is one of the major plant hormones that promote parthenocarpy,a highly valuable agronomic ***,we demonstrated that exogenous GA3application triggered the formation of parthenocarpic fruits with smaller size but unchanged shape in tomato(Solanum lycopersicum).These fruits exhibited a thicker pericarp,undeveloped ovules,and few jelly tissues,leading to smaller locules with empty *** investigation showed that GA treatment produced more cell layers with larger cells in the pericarp,suggesting its promotion in both cell division and *** analyses between GA-3and mock-treated unpollinated ovaries/fruits identified a large number of differentially expressed genes related to hormones,cell division,cell expansion,and transcription factors,implying that they coordinately regulated parthenocarpy conferred by *** particular,the downregulation of five reported repressors of tomato parthenocarpy,including two auxin signaling components,AUXIN RESPONSE FACTOR5(SlARF5)and Sl ARF7,and three MADS-box genes,TOMATO APETALA3(TAP3),TOMATO PISTILLATA(TPI),and AGAMOUS-LIKE6(SlAGL6),after GA treatment might play a key role in this ***,we found that the knockdown of a GA signaling factor SlMYB33,which was depressed by GA treatment,induced parthenocarpic fruit set in tomato,an effect that might have been achieved by enhancing GA biosynthesis and decreasing the expression of some repressors of tomato ***,our results provide a basis for understanding the regulatory mechanism of GA in tomato parthenocarpy.

关键词： Tomato Gibberellin Parthenocarpy Histology Transcriptome SlMYB33

MEMS Huygens Clock Based on Synchronized Micromechanical Resonators

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Engineering 2024年第5期36卷 124-131页

作者： Xueyong Wei Mingke Xu Qiqi Yang Liu Xu a Yonghong Qi Ziming Ren Juan Ren Ronghua Huan Zhuangde Jiang State key Laboratory for Manufacturing System Engineering Xi’an Jiaotong UniversityXi’an 710049China School of Construction Machinery Chang’an UniversityXi’an 710064China Department of Mechanics Zhejiang UniversityHangzhou 310027China

With the continuous miniaturization of electronic devices,microelectromechanical system(MEMS)oscillators that can be combined with integrated circuits have attracted increasing *** study reports a MEMS Huygens clock based on the synchronization principle,comprising two synchronized MEMS oscillators and a frequency compensation *** MEMS Huygens clock improved shorttime stability,improving the Allan deviation by a factor of 3.73 from 19.3 to 5.17 ppb at 1 s.A frequency compensation system based on the MEMS oscillator’s temperature-frequency characteristics was developed to compensate for the frequency shift of the MEMS Huygens clock by controlling the resonator *** effectively improved the long-term stability of the oscillator,with the Allan deviation improving by 1.6343105 times to 30.9 ppt at 6000 *** power consumption for compensating both oscillators simultaneously is only 2.85 mW·℃^(-1).Our comprehensive solution scheme provides a novel and precise engineering solution for achieving high-precision MEMS oscillators and extends synchronization applications in MEMS.

关键词： Frequency stability Huygens clock MEMS Oscillator Synchronization

Remodeling the tumor microenvironment by vascular normalization and GSH-depletion for augmenting tumor immunotherapy

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Chinese Chemical Letters 2024年第2期35卷 329-334页

作者： Jin Wang Qingqing Zhang Yanchen Li Xiaoyan Pan Yuanyuan Shan Jie Zhang School of Pharmacy Health Science CenterXi'an Jiaotong UniversityXi'an 710061China Department of Pharmacy The First Affiliated Hospital of Xi'an Jiaotong UniversityXi'an 710061China

Remodeling tumor microenvironment(TME)is a very promising and effective strategy to enhance the effects of chemotherapy,photodynamic therapy,and *** of tumor vasculature as well as depletion of glutathione(GSH)can improve the ***,we developed a novel therapeutic nanoparticle functional enzyme ultra QDAU5 nanoparticles(FEUQ Nps)based on a fluorescence-on and releasable strategy by combining a vascular normalization inducer,a GSH depleting agent,and an activated *** which the cleavage of disulfide bonds releases active molecules that induce vascular normalization and improve the hypoxic *** addition,it may deplete GSH in cancer cells,thus inducing the production of reactive oxygen species(ROS)and lipid peroxide(LPO)and promoting iron *** may also lead to endoplasmic stress and release of calmodulin,which activates the immune ***,quenched fluorophores are turned on in the presence of galactosidase(GLU)for tumor-specific *** summary,we developed novel therapeutic agent nanoparticles with the function of vascular normalization inducers to achieve specific labeling of hepatocellular carcinoma while exerting efficient antitumor effects in vivo.

关键词： Vascular normalization Tumor microenvironment GSH depletion Ferroptosis Activated immune

3D printing in space:from mechanical structures to living tissues

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International Journal of Extreme Manufacturing 2024年第2期6卷 378-387页

作者： Mao Mao Zijie Meng Xinxin Huang Hui Zhu Lei Wang Xiaoyong Tian Jiankang He Dichen Li Bingheng Lu State key Laboratory for Manufacturing Systems Engineering Xi’an Jiaotong UniversityXi’an 710049People’s Republic of China National Medical Products Administration(NMPA)key Laboratory for Research and Evaluation of Additive Manufacturing Medical Devices Xi’an Jiaotong UniversityXi’an 710049People’s Republic of China National Innovation Platform(Center)for Industry-Education Integration of Medical Technology Xi’an Jiaotong UniversityXi’an 710049People’s Republic of China Frontier Institute of science and Technology Xi’an Jiaotong UniversityXi’an 710049People’s Republic of China National Innovation Institute of Additive Manufacturing Xi’an 710000People’s Republic of China

3D printing stands at the forefront of transforming space exploration,offering unprecedented on-demand and rapid manufacturing *** adeptly addresses challenges such as mass reduction,intricate component fabrication,and resource *** the obstacles posed by microgravity and extreme environments,continual advancements underscore the pivotal role of 3D printing in aerospace *** its primary function of producing space structures,3D printing contributes significantly to progress in electronics,biomedicine,and resource *** perspective delves into the technological advantages,environmental challenges,development status,and opportunities of 3D printing in *** its crucial impact,we anticipate that 3D printing will unlock innovative solutions,reshape manufacturing practices,and foster self-sufficiency in future space endeavors.

关键词： 3D printing in space space manufacturing microgravity

A new insight into LPSO phase transformation and mechanical properties uniformity of large-scale Mg-Gd-Y-Zn-Zr alloy prepared by multi-pass friction stir processing

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Journal of Magnesium and Alloys 2024年第5期12卷 2041-2056页

作者： Xiaohu Guan Wen Wang Ting Zhang Pai Peng Qiang Liu Peng Han Ke Qiao Jun Cai Liqiang Wang Kuaishe Wang School of Metallurgical Engineering National and Local Joint Engineering Research Center for Functional Materials ProcessingXi’an University of Architecture and TechnologyNo.13 Yanta RoadXi’anShaanxi 710055China Xi’an Rare Metal Materials Institute Co.Ltd Xi’an 710016China State key Laboratory of Metal Matrix Composites School of Material Science and EngineeringShanghai Jiao Tong UniversityNo.800 Dongchuan RoadShanghai 200240China

A large-scale fine-grained Mg-Gd-Y-Zn-Zr alloy plate with high strength and ductility was successfully prepared by multi-pass friction stir processing(MFSP)technology in this *** structure of grains and long period stacking ordered(LPSO)phase were characterized,and the mechanical properties uniformity was ***,a quantitative relationship between the microstructure and tensile yield strength was *** results showed that the grains in the processed zone(PZ)and interfacial zone(IZ)were refined from 50μm to 3μm and 4μm,respectively,and numerous original LPSO phases were *** IZ,some block-shaped 18R LPSO phases were transformed into needle-like 14H LPSO phases due to stacking faults and the short-range diffusion of solute *** severe shear deformation in the form of kinetic energy caused profuse stacking fault to be generated and move rapidly,greatly increasing the transformation rate of LPSO *** MFSP,the ultimate tensile strength,yield strength and elongation to failure of the large-scale plate were 367 MPa,305 MPa and 18.0% *** refinement and LPSO phase strengthening were the major strengthening mechanisms for the MFSP *** particularly,the strength of IZ was comparable to that of PZ because the strength contribution of the 14H LPSO phase offsets the lack of grain refinement strengthening in *** result opposes the widely accepted notion that IZ is a weak region in MFSP-prepared large-scale fine-grained plate.

关键词： Friction stir processing Multi-pass Mg-Gd-Y-Zn-Zr alloy LPSO phase transformation Mechanical properties

Transfer film effects induced by 3D-printed polyether-ether-ketone with excellent tribological properties for joint prosthesis

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Bio-Design and Manufacturing 2024年第1期7卷 43-56页

作者： Yang Li Jibao Zheng Changning Sun Dichen Li State key Laboratory for Manufacturing System Engineering School of Mechanical EngineeringXi’an Jiaotong UniversityXi’an 710054China National Medical Products Administration(NMPA)key Laboratory for Research and Evaluation of Additive Manufacturing Medical Devices Xi’an Jiaotong UniversityXi’an 710054China

Based on the building principle of additive manufacturing,printing orientation mainly determines the tribological properties of joint *** this study,we created a polyether-ether-ketone(PEEK)joint prosthesis using fused filament fabrication and investigated the effects of printing orientation on its tribological properties using a pin-on-plate tribometer in 25% newborn calf *** ultrahigh molecular weight polyethylene transfer film is formed on the surface of PEEK due to the mechanical capture of wear debris by the 3D-printed groove morphology,which is significantly impacted by the printing orientation of *** the printing orientation was parallel to the sliding direction of friction,the number and size of the transfer film increased due to higher steady *** transfer film protected the matrix and reduced the friction coefficient and wear rate of friction pairs by 39.13%and 74.33%,***,our findings provide a novel perspective regarding the role of printing orientation in designing knee prostheses,facilitating its practical applications.

关键词： 3D printing orientation Transfer film Tribological properties Polyether-ether-ketone Knee prosthesis