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Analysis and Characterization of the GABA Transaminase and Succinate Semialdehyde Dehydrogenase Genes in the Microalga Isochrysis zhanjiangensis in Response to Abiotic Stresses

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Journal of Ocean University of China 2024年第3期23卷 775-785页

作者： TIAN Jiaojiao ZHANG Lin LU Xinyue YE Lingzhi WU Yuanyuan CHEN Deshui CAO Jiayi JIANG Jiaxin XU Jilin YAN Xiaojun Key Laboratory of Marine Biotechnology of Zhejiang Province School of Marine SciencesNingbo UniversityNingbo 315211China Key Laboratory of Aquacultural Biotechnology Ministry of Education Ningbo UniversityNingbo 315211China Laizhou Bay Marine Technology Co. Ltd.Yantai 261400China Fujian Dalai Seed Science and Technology Co. Ltd.Ningde 352101China

Gamma-aminobutyric acid(GABA),widely existing in different organisms,is rapidly accumulated in plants in response to environmental *** main biosynthesis and degradation pathways of GABA constitute the GABA shunt,which is tied to the tricarboxylic acid(TCA)*** transaminase(GABA-T)and succinate semialdehyde dehydrogenase(SSADH)are two essential enzymes for the GABA degradation *** there are abundant studies on GABA shunt in higher plants at the physiological and genetic levels,research on its role in microalgae remains *** study aimed at exploring the function of GABA-T and SSADH genes in Isochrysis zhanjiangensis,an important diet microalga,under different *** cloned two GABA-T genes,IzGABA-T1 and IzGABA-T2,and one SSADH gene IzSSADH from Isochrysis zhanjiangensis and conducted heterologous expression *** results showed that the overexpression of IzGABA-T1 or IzGABA-T2 enhanced the survival rates of yeast transformants under heat or NaCl stress,while the overexpression of IzSSADH improved yeast tolerance to NaCl stress but had no obvious effect on heat ***,the results of quantitative real-time polymerase chain reaction(qPCR)showed that IzGABA-T1 transcription increased in the HT(salinity 25,35℃)and LS(salinity 15,25℃)*** 24 h,the IzGABA-T2 transcriptions increased in the HT,LS,and HS(salinity 35,25℃)groups,but their transcription levels decreased in all groups at 48 *** transcription increased in the LS *** results suggest that IzGABA-T1,IzGABA-T2,and IzSSADH are associated with temperature and salinity stresses and possess a certain preference for different stresses.

关键词： abiotic stress GABA heterologous expression Isochrysis zhanjiangensis transcription

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Post-buckling Reliability and Sensitivity Analysis of Composite Stiffened Plates Based on Adaptive Kriging Method

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Acta Mechanica Solida Sinica 2023年第2期36卷 340-348页

作者： Binwen Wang Lilong Luo Xiaohua Nie Shihui Duan Likai Wang Aircraft Strength research Institute of China Xi’an 710065China School of Civil Aviation Northwestern Polytechnical UniversityXi’an 710072China

Based on the active learning Kriging(ALK)model and the Hashin failure criterion,this paper proposes a new reliability evaluationmodel for composite stiffened panels,and conducts a reliability analysis on the ultimate bearing *** addition,this paper studies the importance ranking of input *** comparing the calculation results of the reliability model proposed in this paper with those of the Monte Carlo method,the accuracy and efficiency of the ALK model are verified by case ***,the effects of longitudinal elastic modulus and fiber-direction tensile strength on post-buckling failure probability are discussed,which provides significant reference and guidance for the optimization and design of composite stiffened plates.

关键词： Post-bulking reliability analysis Sensitivity analysis Composite stiffened plates Active learning Kriging Hashin failure criterion

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Study on the clinical significance of TRPV2 and MMP2 expressions in ovarian cancer

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BIOCELL 2021年第3期45卷 521-526页

作者： XIAOPANG REN WANJIAO HAO JIN LIU YE LI BING WANG XINHUI ZU HUILING XUE Department of Obstetrics and Gynecology Affiliated Hospital of Hebei UniversityBaoding071000China

Ovarian cancer was one of the most common malignant tumors in female reproductive ***,epithelial ovarian cancer showed the highest mortality rate in gynecological tumors,posing serious threats to women’s life and *** Receptor Potential Cation Channel Subfamily V Member 2(TRPV2)and matrix metalloproteinase-2 MMP-2 have been found to play important roles in regulating the pathogenesis of most tumors,but there were few studies exploring the relationships of TRPV2 and MMP-2 in ***,we evaluated the expression of TRPV2 and MMP-2 proteins in cancer tissues and adjacent normal tissues of OC *** was used to analyze the expressions of TRPV2 and MMP-2 in cancer tissues(N=70)and adjacent normal tissues(N=70)of OC patients,and the correlation of TRPV2 and MMP-2 with the occurrence and development of OC was analyzed with the combination of clinicopathological parameters of OC *** results showed that the expressions of TRPV2 and MMP-2 in OC tissues were significantly higher than those in adjacent normal tissues,and there were significant differences in TRPV2 and MMP-2 expressions in terms of tumor stage,differentiation,and lymph node metastasis of *** together,our results showed that protein expressions of TRPV2 and MMP-2 were closely related associated with the occurrence and development of OC.

关键词： Ovarian cancer TRPV2 MMP-2 Immunohistochemistry

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Broadband Fourier-Domain Mode-Locked Lasers

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Photonic Sensors 2011年第3期1卷 222-227页

作者： Kevin HSU Panomsak MEEMON Kye-Sung LEE Peter J. DELFYETT Jannick E ROLLAND Micron Optics Inc. Atlanta Georgia 30345 USA CREOL The College of Optics and Photonics University of Central Florida Orlando Florida 32816 USA The Institute of Optics University of Rochester Rochester New York 14627 USA

Broadband, high-speed wavelength-swept lasers can substantially enhance applications in optical coherence tomography, chemical spectroscopy, and fiber-optic sensing. We report the demonstration of Fourier-domain mode-lock lasers operating at about 90kHz effective sweep rate over a 158nm sweep range using a single-band design and over a 284nm sweep range across the 1.3 μm to 1.5μm wavelength spectrum using a unique broadband design. A novel dual-detection full-range Fourier-domain optical coherence tomography system is developed which provides 7 I-tm axial resolution （in air）at about 90 kHz axial scan rate for mirror-image resolved Doppler imaging in a human finger and an African frog tadpole.

关键词： Fourier-domain mode-locked laser swept laser tunable laser

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Identification and Functional Characterization of a NovelΔ12 Fatty Acid Desaturase Gene from Haematococcus pluvialis

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Journal of Ocean University of China 2020年第6期19卷 1362-1370页

作者： ZHANG Lin CHEN Wenbi YANG Shuping ZHANG Yuanbo XU Jilin YANG Dongjie WU Zuyao LIU Tong CAO Jiayi School of Marine Sciences Ningbo UniversityNingbo 315211China College of Food and Pharmaceutical Sciences Ningbo UniversityNingbo 315211China

The freshwater microalga Haematococcus pluvialis accumulates large amounts of fatty acids in response to adverse ***,the key fatty acid desaturase genes in *** remain *** this study,we cloned and functionally characterized aΔ12 fatty acid desaturase gene,and designated it as *** open reading frame of HpFAD2 consisted of 1137 base pairs and encoded 378 amino *** deduced polypeptide showed 70%identity to other endoplasmic reticulumΔ12 fatty acid desaturases,whereas it had only 44%identity to plastidΔ12 fatty acid *** PSORT algorithm and phylogenetic analysis further confirmed its affiliation to the endoplasmic reticulumΔ12 fatty acid *** expression was performed in Saccharomyces cerevisiae cells transformed with the recombinant plasmid *** additional fatty acids(C16:2 and C18:2)were detected in the yeast *** results indicatedΔ12 desaturation activity and substrate preference for C18:1 over C16:*** transcriptional levels of *** HpFAD2 at different growth stages were measured by quantitative polymerase chain reaction(PCR),indicating that the HpFAD2 transcriptional levels were significantly higher in red cells than those in green *** study brings more insight into the fatty acid biosynthetic pathway of ***.

关键词： Δ12 fatty acid desaturase fatty acid Haematococcus pluvialis Saccharomyces cerevisiae transcriptional level

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Advances in nuclear detection and readout techniques

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Nuclear Science and Techniques 2023年第12期34卷 281-358页

作者： Rui He Xiao‑Yang Niu Yi Wang Hong‑Wei Liang Hong‑Bang Liu Ye Tian Hong‑Lin Zhang Chao‑Jie Zou Zhi‑Yi Liu Yun‑Long Zhang Hai‑Bo Yang Ju Huang Hong‑Kai Wang Wei‑Jia Han Bei Cao Gang Chen Cong Dai Li‑Min Duan Rui‑Rui Fan Fang‑Fa Fu Jian‑Hua Guo Dong Han Wei Jiang Xian‑Qin Li Xin Li Zhuo‑Dai Li Yu‑Tie Liang Shun Liao De‑Xu Lin Cheng‑Ming Liu Guo‑Rui Liu Jun‑Tao Liu Ze Long Meng‑Chen Niu Hao Qiu Hu Ran Xiang‑Ming Sun Bo‑Tan Wang Jia Wang Jin‑Xiang Wang Qi‑Lin Wang Yong‑Sheng Wang Xiao‑Chuan Xia Hao‑Qing Xie He‑Run Yang Hong Yin Hong Yuan Chun‑Hui Zhang Rui‑Guang Zhao Ran Zheng Cheng‑Xin Zhao Institute of Modern Physics Chinese Academy of SciencesLanzhou 730000China School of Nuclear Science and Technology University of Chinese Academy of SciencesBeijing 100049China Advanced Energy Science and Technology Guangdong Laboratory Huizhou 516000China Key Laboratory of Particle and Radiation Imaging Department of Engineering PhysicsTsinghua UniversityBeijing 100084China School of Integrated Circuits Dalian University of TechnologyDalian 116020China Guangxi Key Laboratory for Relativistic Astrophysics School of Physical Science and TechnologyGuangxi UniversityNanning 530004China School of Nuclear Science and Technology Lanzhou UniversityLanzhou 730000China State Key Laboratory of Particle Detection and Electronics University of Science and Technology of ChinaHefei 230026China Department of Radiation Oncology National Cancer Center/National Clinical Research Center for Cancer/Cancer HospitalChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100021China Electronic Engineering School Heilongjiang UniversityHarbin 150080China Spallation Neutron Source Science Center Dongguan 523803China Micro‑electronic Department Harbin Institute of TechnologyHarbin 150001China Purple Mountain Observatory Chinese Academy of SciencesNanjing 210034China Liaoning Academy of Materials Shenyang 110167China Istituto Nazionale di Fisica Nucleare Sezione di PerugiaPerugiaItaly School of Mathematics and Physics Chengdu University of TechnologyChengdu 620035China PLAC Key Laboratory of Quark and Lepton Physics(MOE)Central China Normal UniversityWuhan 430079China School of Electronics and Information Northwestern Polytechnical UniversityXi’an 710129China State Key Lab of Superhard Materials College of PhysicsJilin UniversityChangchun 130012China School of Computer Science Northwestern Polytechnical UniversityXi’an 710129China

“A Craftsman Must Sharpen His Tools to Do His Job,”said *** detection and readout techniques are the foundation of particle physics,nuclear physics,and particle astrophysics to reveal the nature of the ***,they are being increasingly used in other disciplines like nuclear power generation,life sciences,environmental sciences,medical sciences,*** article reviews the short history,recent development,and trend of nuclear detection and readout techniques,covering Semiconductor Detector,Gaseous Detector,Scintillation Detector,Cherenkov Detector,Transition Radiation Detector,and Readout *** explaining the principle and using examples,we hope to help the interested reader underst and this research field and bring exciting information to the community.

关键词： Silicon detector Wide bandgap detector Time projection chamber(TPC) Multigap resistive plate chamber detector(MRPC) Scintillation detector Calorimeters Cherenkov detector Transition radiation detector Artificial intelligence Trigger-less readout

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