检索结果-南通市图书馆

Synergy between Cucumber Mosaic Virus and Zucchini Yellow Mosaic Virus on Cucurbitaceae Hosts Tested by Real-time Reverse Transcription-Polymerase Chain Reaction

引用

Acta Biochimica et Biophysica Sinica 2007年第6期39卷 431-437页

作者： Rong ZENG~(1,2#), Qiansheng LIAO~(2#), Junli FENG~2, Dingjun LI~1, and Jishuang CHEN~(2*) 1 College of Bio-Safety Science Technology, Hunan Agricultural University, Changsha 410128, China, 2 Institute of Bioengineering, Zhejiang Sci-Teeh University, Hangzhou 310018, China College of Bio-Safety Science Technology Human Agricultural UniversityChangsha 410128 China Institute of Bioengineering Zhejiang Sci-Tech UniversityHangzhou 310018 China

Cucumber mosaic virus (CMV) and zucchini yellow mosaic virus (ZYMV) are two principal viruses infecting cucurbitaceous crops, and their synergy has been repeatedly observed. In our present work, a real-time reverse transcription-polymerase chain reaction procedure was established to study the accumulation kinetics of these two viruses in single and combined infections at the molecular level. The accumulations of open reading frames (ORFs) for 1a, 2a, 3a and coat protein (CP) of CMV and CP of ZYMV were tested. In the single infection, CMV-Fny ORFs accumulated to their maxima in cucumber or bottle gourd at 14 d post-inoculation (dpi), and gradually declined thereafter. ZYMV-SD CP ORF reached maximal accumulation at 14 and 28 dpi on cucumber and bottle gourd, respectively. However, when coinfected with CMV-Fny and ZYMV-SD, the maximal accumulation levels of all viral ORFs were delayed. CMV-Fny ORFs reached their maxima at 21 dpi on both hosts, and ZYMV-SD CP ORF reached maximal accumulation at 21 and 28 dpi on cucumber and bottle gourd, respectively. Generally, the accumulation levels of CMV-Fny ORFs in the co-infection were higher than those in the single infection, whereas the accumulation of ZYMV-SD CP ORF showed a reverse result.

关键词： cucumber mosaic virus genornic RNA real-time RT-PCR relative quantification synergy zucchini yellow mosaic virus

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Identification of human dopamine receptors agonists from Chinese herbs

引用

Acta Pharmacologica Sinica 2007年第1期28卷 132-139页

作者： Yi-lin ZHANG~(1,2,3,4) Hai-qing ZHANG~(1,2,3,4) Xiao-yu LIU~3 Shi-neng HUA~3 Lu-bing ZHOU~3 Jun YU~2 Xue-hai TAN~(3,5)1 Graduate School of the Chinese Academy of Sciences,Beijing 100039,China 2 Beijing Genomics Institute,Chinese Academy of Sciences Beijing 101300,China 3 HD Biosciences Co Ltd,Shanghai 201203,China Graduate School of the Chinese Academy of Sciences Beijing China Beijing Genomics Institute Chinese Academy of Sciences Beijing China HD Biosciences Co Ltd Shanghai China

Aim:To find human dopamine receptors,especially Dl-like receptor specific ago-nists from Chinese herbs as potential antihypertension drug ***:TwoD l-like receptor cell lines carrying a μ-lactamase reporter gene,and a D2 receptorcell line coexpressing a promiscuous G protein G15 were constructed using HEK293cells.A natural compound library made from fractionated samples of herbal ex-tracts was used for high-throughput screening（HTS）against one of the cell lines,HEK/D5R/*** interested hits were evaluated for their activities againstvarious dopamine ***:Fourteen hits were identified from primaryscreening,of which 2 of the better hit samples,HD0522 and HD0059,were selectedfor further material and activity analysis,and to obtain 2 compounds that ap-peared as 2 single peaks in HPLC,HD0522H01 and ***0059H01could activate D 1,D2,and D5 receptors,with EC50values of 2.28 μg/mL,0.85 μg/mL,and 1.41 μg/mL,***0522H01 could only activate DlR and D5Rwith EC50values of 2.95 μg/mL and 8.38 μg/***:We established cell-based assays for 3 different human dopamine receptors and identified specificagonists HD0522H01 and HD0059H01 through *** specific agonist to D1-like receptors,HD0522H01,may become a new natural product-based drug leadfor antihypertension treatment.

关键词： dopamine receptors natural products high throughput screening hypertension

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Jasmonic Acid is Induced in a Biphasic Manner in Response of Pea Seedlings to Wounding

引用

Journal of Integrative Plant Biology 2009年第6期51卷 562-573页

作者： Hao-Ru Yang Ke Tang Hong-Tao Liu Qiu-Hong Pan Wei-Dong Huang College of Food Science and Nutritional Engineering China Agricultural University Beijing 100083 China Institute of Geographic Sciences and Natural Resources Research the Chinese Academy of Sciences Beijing 100101 China

The role of jasmonic acid （JA） in plant wounding response has been demonstrated. However, the source of JA in wound signaling remains unclear. In the present study, pea seedlings were used as material to investigate the systemic induction of JA and the activation of lipoxygenase （LOX）-dependent octadecanoid pathway upon wounding. The results showed that endogenous JA could induce two peaks in the wounded leaves and the stalks, while only one peak in the systemic leaves. LOX activity and its protein amount were also induced and the stimulation mainly occurred in the late phase, while one peak of induction was present after pretreatment with JA. Applied nordihydroguaiaretic acid （NDGA）, an inhibitor of LOX activity, only inhibited the induction of JA in the late phase, and the resistance of pea was impaired. Furthermore, 13（S）- hydroperoxy-9（Z）, 11 （E）-octadecadienoic acid （13（S）-H（P）ODE） was confirmed to be the main product of LOX throughout the experimental time. in addition, immunocytochemical analysis also revealed the occurrence of JA biosynthesis and transport upon wounding. These results demonstrated that wound-induced JA in wounded leaves resulted from its biosynthesis and conversion from its conjugates, while in systemic leaves resulted from its transport and biosynthesis; and proved that the LOX pathway was vital to the wound-induced defense response involved in JA biosynthesis.

关键词： defense response jasmonic acid lipoxygenase pea (Pisum sativum L.).

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

A double-edged sword:ROS related therapies in the treatment of psoriasis

引用

Asian Journal of Pharmaceutical Sciences 2022年第6期17卷 798-816页

作者： Jingyi Hu Qiong Bian Xiaolu Ma Yihua Xu Jianqing Gao Institute of Pharmaceutics College of Pharmaceutical SciencesZhejiang UniversityHangzhou 310058China College of Pharmacy Inner Mongolia Medical UniversityHohhot 010000China Jiangsu Engineering Research Center for New-type External and Transdermal Preparations Changzhou 213149China

In the onset and progression of psoriasis,redox imbalance is a vital ***'s widely accepted that too much reactive oxygen species(ROS)always make psoriasis *** research,however,has shown that the accumulation of ROS is not entirely detrimental,as it helps reduce psoriasis lesions by inhibiting epidermal proliferation and keratinocyte *** a result,ROS appears to have two opposing effects on the treatment of *** this review,the current ROS-related therapies for psoriasis,including basic and clinical research,are ***,the design and therapeutic benefits of various drug delivery systems and therapeutic approaches are examined,and a potential balance between antioxidative stress and ROS accumulation is also trying to be investigated.

关键词： Psoriasis ROS related therapies Antioxidant ROS accumulation

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Performance evaluation of deep neural networks for forecasting time-series with multiple structural breaks and high volatility

引用

CAAI Transactions on Intelligence Technology 2021年第3期6卷 265-280页

作者： Rohit Kaushik Shikhar Jain Siddhant Jain Tirtharaj Dash Department of Computer Science and Information Systems Birla Institute of Technology and Science PilaniK.K.Birla Goa CampusZuarinagarGoaIndia

The problem of automatic and accurate forecasting of time-series data has always been an interesting challenge for the machine learning and forecasting community.A majority of the real-world time-series problems have non-stationary characteristics that make the understanding of trend and seasonality *** applicability of the popular deep neural networks(DNNs)as function approximators for non-stationary TSF is *** following DNN models are evaluated:Multi-layer Perceptron(MLP),Convolutional Neural Network(CNN),and RNN with Long Short-Term Memory(LSTM-RNN)and RNN with Gated-Recurrent Unit(GRU-RNN).These DNN methods have been evaluated over 10 popular Indian financial stocks ***,the performance evaluation of these DNNs has been carried out in multiple independent runs for two settings of forecasting:(1)single-step forecasting,and(2)multi-step *** DNN methods show convincing performance for single-step forecasting(one-day ahead forecast).For the multi-step forecasting(multiple days ahead forecast),the methods for different forecast periods are *** performance of these methods demonstrates that long forecast periods have an adverse effect on performance.

关键词： networks neural forecasting

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Generating barcodes for nanopore sequencing data with PRO

引用

Fundamental Research 2024年第4期4卷 785-794页

作者： Ting Yu Zitong Ren Xin Gao Guojun Li Renmin Han Research Center for Mathematics and Interdisciplinary Sciences Frontiers Science Center for Nonlinear Expectations(Ministry of Education)Shandong UniversityShandong 266000China Computer Electrical and Mathematical Sciences and Engineering Division&Computational Bioscience Research CenterKing Abdullah University of Science and TechnologyThuwal 23955Saudi Arabia

DNA barcodes,short and unique DNA sequences,play a crucial role in sample identification when processing many samples simultaneously,which helps reduce experimental ***,the low quality of long-read sequencing makes it difficult to identify barcodes accurately,which poses significant challenges for the design of barcodes for large numbers of samples in a single sequencing ***,we present a comprehensive study of the generation of barcodes and develop a tool,PRO,that can be used for selecting optimal barcode sets and *** formulate the barcode design problem as a combinatorial problem and prove that finding the optimal largest barcode set in a given DNA sequence space in which all sequences have the same length is theoretically *** practical applications,we developed the novel method PRO by introducing the probability divergence between two DNA sequences to expand the capacity of barcode kits while ensuring demultiplexing ***,the maximum size of the barcode kits designed by PRO is 2,292,which keeps the length of barcodes the same as that of the official ones used by Oxford Nanopore Technologies(ONT).We validated the performance of PRO on a simulated nanopore dataset with high error *** demultiplexing accuracy of PRO reached 98.29%for a barcode kit of size 2,922,4.31%higher than that of Guppy,the official demultiplexing *** the size of the barcode kit generated by PRO is the same as the official size provided by ONT,both tools show superior and comparable demultiplexing accuracy.

关键词： Third-generation sequencing Nanopore sequencing DNA barcode Farthest point sampling algorithm High throughput

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Multiple valence states of Fe boosting SERS activity of Fe_(3)O_(4) nanoparticles and enabling effective SERS-MRI bimodal cancer imaging

引用

Fundamental Research 2024年第4期4卷 858-867页

作者： Jie Lin Xuehua Ma Anran Li Ozioma Udochukwu Akakuru Chunshu Pan Meng He Chenyang Yao Wenzhi Ren Yanying Li Dinghu Zhang Yi Cao Tianxiang Chen Aiguo Wu Cixi Institute of Biomedical Engineering International Cooperation Base of Biomedical Materials Technology and ApplicationChinese Academy of Science Key Laboratory of Magnetic Materials and DevicesZhejiang Engineering Research Center for Biomedical MaterialsNingbo Institute of Materials Technology and EngineeringCASNingbo315201China School of Engineering Medicine Key Laboratory of Big Data-Based Precision Medicine(Beihang University)Ministry of Industry and Information TechnologyBeihang UniversityBeijing100191China Advanced Energy Science and Technology Guangdong Laboratory Huizhou 516000China University of Chinese Academy of Sciences Bejing 100049China

Developing novel nanoparticle-based bioprobes utilized in clinical settings with imaging resolutions ranging from cell to tissue levels is a major challenge for tumor diagnosis and ***,an optimized strategy for designing a Fe_(3)O_(4)-based bioprobe for dual-modal cancer imaging based on surface-enhanced Raman scattering(SERS)and magnetic resonance imaging(MRI)is *** SERS activity of ultrasmall Fe_(3)O_(4) nanoparticles(NPs)was discovered,and a 5×10^(-9)M limit of detection for crystal violet molecules was successfully *** high-efficiency interfacial photon-induced charge transfer in Fe_(3)O_(4) NPs was promoted by multiple electronic energy levels ascribed to the multiple valence states of Fe,which was observed using ultraviolet-visible diffuse reflectance *** functional theory calculations were utilized to reveal that the narrow band gap and high electron density of states of ultrasmall Fe_(3)O_(4) NPs significantly boosted the vibronic coupling resonances in the SERS system upon *** subtypes of cancer cells were accurately recognized via high-resolution SERS imaging in vitro using the prepared Feg Og-based bioprobe with high sensitivity and good ***,Fe_(3)O_(4)-based bioprobes simultaneously exhibited T,-weighted MRI contrast enhancement with an active targeting capability for tumors in *** the best of our knowledge,this is the first report on the use of pure semiconductor-based SERS-MRI dual-modal nanoprobes in tumor imaging in vivo and in vitro,which has been previously realized only using semiconductor-metal complex *** non-metallic materials with SERS-MRI dual-modal imaging established in this report are a promising cancer diagnostic platform,which not only showed excellent performance in early tumor diagnosis but also possesses great potential for image-guided tumor treatment.

关键词： Fe_(3)O_(4)NPs SERS-MRI dual-modal nanoprobes PICT Multiple valence states Cancer diagnosis

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Ultrasound heterogeneity as an indicator of testicular salvage in testicular torsion: A single center experience

引用

Asian Journal of Urology 2022年第1期9卷 57-62页

作者： Omran Hasan Mohamed Mubarak S.Mohamed Jawad Alwedaie Hasan Baksh Husain Alaradi Ameer Alarayedh Ali Alaradi Abdolsalam Ahmadi Akbar Jalal Department of Surgery Salmaniya Medical Complex Ringgold Standard InstitutionManamaBahrain

Objective:Testicular torsion is a common urological emergency,mainly in the younger *** aim is to evaluate the efficacy and value of testicular echogenicity in predicting testicular ***:Patients who were older than 13 years of age and were discharged with the diagnosis of testicular torsion were *** obtained included demographic data,history,physical examination results,ultrasound findings including size,color Doppler flow,and echotexture,torsion degrees,and surgical *** addition,the histopathological findings of patients who underwent orchiectomy were ***:A total of 71 cases were diagnosed with testicular torsion between January 2016 to December *** mean age at presentation was 20.4±7.0 *** average time from the onset of pain until the presentation was 36.0±55.1 *** of the 71 ultrasound scans reviewed,45.1%showed homogenous echotexture and 54.9%showed heterogeneous echotexture of the affected *** scrotal exploration,the mean degree of torsion was 475.7±301.8 degrees;66.2%of the patients had a viable testis;and bilateral orchidopexy was *** remaining 33.8%had a non-viable *** echotexture was after an average of 13.5 h of scrotal pain,while heterogeneous echotexture presented after 53.7 h of scrotal *** was a statistically significant difference between the time of presentation and echotexture changes on scrotal ultrasonography(p<0.01).The relationship between echotexture changes and testicular viability was statistically significant as well(p<0.001).Conclusion:A long time since the onset of pain coupled with heterogeneous changes in testicular parenchyma is a good indicator of ***,we still advocate for surgical exploration as the gold standard in diagnosis and management.

关键词： Testis Testicular torsion Orchiopexy Orchiectomy Ultrasound Echotexture

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Activity and stability of bacterial cellulase immobilized on magnetic nanoparticles

引用

Chinese Journal of Catalysis 2016年第11期37卷 1891-1898页

作者： Kandasamy Selvam Muthusamy Govarthanan Duraisamy Senbagam Seralathan Kamala-Kannan Balakrishnan Senthilkumar Thangasamy Selvankumar Muthayammal艺术与科学学院生物工程中心印度泰米尔纳德邦纳马卡尔637408 Mahendra艺术与科学学院生物工程系印度泰米尔纳德邦纳马卡尔637501 室兰工业大学环境工程学院应用科学系日本北海道620024 巴拉迪大学海洋生物工程系印度泰米尔纳德邦蒂鲁吉拉伯利620024 全北国立大学环境和生物资源科学学院环境与生物科学高级研究所生物技术部韩国益山570752 Haramaya大学健康与医学科学学院医学微生物学系埃塞俄比亚哈拉尔

Magnetic nanoparticles（Fe3O4） were synthesized by co-precipitating Fe^2＋ and Fe^3＋ ions in an ammonia solution and treating under hydrothermal *** was immobilized onto Fe3O4 magnetic nanoparticles via glutaraldehyde *** response surface methodology and Box-Behnken design,the variables such as magnetic nanoparticle concentration,glutaraldehyde concentration,enzyme concentration,and cross linking time were *** Box-Behnken design analysis showed a reasonable adjustment of the quadratic model with the experimental *** contour plots were generated to evaluate the changes in the response surface and to understand the relationship between the nanoparticles and the enzyme *** electron microscopy,X-ray diffraction analysis,and Fourier transform infrared spectroscopy were studied to characterize size,structure,morphology,and binding of enzyme onto the *** stability and activity of the bound cellulase was analyzed using various parameters including pH,temperature,reusability,and storage *** immobilized cellulase was compared with free cellulase and it shows enhanced stability and activity.

关键词： Magnetic nanoparticle Immobilized enzyme Response surface methodology Characterization

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Influence of Formate on Bioactivity Material-thuringiensin Synthesized by Bacillus thuringiensis YBT-032

引用

Journal of Wuhan University of Technology(Materials Science) 2008年第5期23卷 691-693页

作者：王志喻子牛 College of Bioengineering Hubei University of Technology State Key Laboratoy of Agricultural Microbiology National Engineering Research Center for Microbial Posticides Huazhong Agricultural University

The biological method to synthesize thuringiensin and the influence of formate on thuringiensin biosynthesis were investigated. Addition of 1.00 g/L formate to growth medium of bacillus thuringiensis YBT-032 resulted in significant enhancements in productions of citrate, α -ketoglutarate, intracellular adenine and thuringiensin. These results demonstrate that added formate attends metabolism of cell, facilitates carbon metabolic flux in tricarboxylic acid cycle and hexose monophosphate pathway. As a carbon source, formate facilitates cell growth, increases glucose consumption and enhances the ability of cell to synthesis adenine analogues, and subsequently thuringiensin. Thuringiensin production rate significantly enhanced from 6.44 to 8.46 mg·g^-1 · h and transformation ratio from glucose to thuringiensin increased by 43.30%.

关键词： bacillus thuringiensis precursor formate thuringiensin

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：