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miR-93 suppresses proliferation and colony formation of human colon cancer stem cells

World Journal of Gastroenterology 2011年第42期17卷 4711-4717页

作者： Xiao-Feng Yu Jian Zou Zhi-Jun Bao Jie Dong Department of Gastroenterology Huadong Hospital Fudan University Shanghai 200040 China

AIM： TO identify differentially expressed microRNAs （miRNAs） in human colon cancer stem cells （SW1116csc） and study their function in SW1116csc proliferation. METHODS： SW1116csc were isolated from the human colon cancer cell line, SW1116 and cultured in serum- free medium. A miRNA microarray was used to detect differential expression profiles of rniRNAs in SW1116csc and SW1116 cells. Real-time quantitative polymerase chain reaction （PCR） was performed to verify the dif- ferential expression of candidate miRNAs obtained from the microarray. Target mRNAs of differentially expressed miRNAs were predicted with target predic- tion tools, miRNA expression plasmids were transfected into SW1116csc using Lipofectamine 2000 reagent. Cell proliferation curves were generated with trypan blue staining, and the colony formation rate of transfected cells was measured with the soft agar colony formation assay. Expression of target mRNAs and proteins from differentially expressed miRNAs were detected using reverse transcription （RT）-PCR and western ***： Compared with expression in SW1116 cells, 35 miRNAs （including hsa-miR-192, hsa-miR-29b, hsa-miR-215, hsa-miR-194, hsa-miR-33a and hsa- miR-32） were upregulated more than 1.5-fold, and 11 miRNAs （including hsa-miR-93, hsa-miR-1231, hsa- miRPlus-F1080, hsa-miR-524-3p, hsa-miR-886-3p and hsa-miR-561） were downregulated in SW1116csc. The miRNA microarray results were further validated with quantitative RT-PCR. miR-93 was downregulated, and its predicted mRNA targets included BAMBI, CCND2, CDKNIA, HDACS, KIF23, MAP3K9, MAP3K11, MYCN, PPARD, TLE4 and ZDHHCl. Overexpressed miR-93 sig- nificantly inhibited cell proliferation and colony forma- tion by SW1116csc. Furthermore, miR-93 negatively regulated the mRNA and protein levels of HDAC8 and TLE4. CONCLUSION： Some miRNAs were differentially ex- pressed during differentiation of SW1116csc into SW1116 cells, miR-93 may inhibit SW1116csc proliferation and colony formation.

关键词： miR-93 Stem cell Colon cancer Expres-sion profile

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Proteome of human colon cancer stem cells:A comparative analysis

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World Journal of Gastroenterology 2011年第10期17卷 1276-1285页

作者： Jian Zou Xiao-Feng Yu Zhi-Jun Bao Jie Dong Department of Gastroenterology Huadong Hospital Fudan UniversityShanghai 200040 China

AIM： To isolate and identify the biological characteristics of human colon cancer stem cells （SW1116 cells） and further study their proteome. METHODS： SW1116 cells were isolated and cultured with a serum-free medium （SFM）. Sphere formation was assayed to observe the formation of colon cancer stem cell spheres. SW1116 cells were inoculated into a serum-containing medium for observing their differentiation characteristics. Proliferation curve and cross-resistance of SWl116 cells to different drugs were detected by MTT. Percentage of SP cells in SW1116 cells was detected with Hoechst33342 staining. Telomerase activity in SW1116cells was checked by polymerase chain reaction （PCR）-enzyme linked immunosorbent assay. Expressions of stem cell relevant genes and proteins were detected by reverse transcription-PCR and Western blot, respectively. Total protein was isolated from SW1116 cells by two-dimensional gel electrophoresis （2-DE） and differentially expressed proteins were identified by tandem mass spectrometry （MALDI-TOF/TOF）. RESULTS： The isolated SW1116 cells presented as spheroid and suspension growths in SFM with a strong self-renewal, proliferation, differentiation and drug-resistance ability. The percentage of SP cells in SW1116 cells was 38.9%. The SW1116 cells co-expressed the CD133 and CD29 proteins. The telomerase activity in SW1116 cells was increased. The expressions of different stem cell relevant genes and proteins were detected. The proteomic analysis showed that the 26 protein spots were differently expressed in SW1116 cells and 10 protein spots were identified as ubiquitin fusion- degradation l-like protein, nuclear chloride channel protein, tubulin 13, Raichu404X, stratifin, F-actin cap- ping protein α-1 subunit, eukaryotic translation elongation factor 1 delta isoform 2, hypothetical protein, glyceraldehyde-3-phosphate dehydrogenase and guanine nucleotide binding protein 13 polypeptide 2-like 1, respectively. CONCLUSION： SW1116 cells are biologically c

关键词： Proteome Stem cell Colon cancer Isola-tion Characterization

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Local asymptotic behavior of regression splines for marginal semiparametric models with longitudinal data

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science China Mathematics 2009年第9期52卷 1982-1994页

作者： QIN GuoYou ZHU ZhongYi Department of Biostatistics School of Public HealthFudan UniversityShanghai 200032China Department of Statistics Fudan UniversityShanghai 200433China

In this paper, we study the local asymptotic behavior of the regression spline estimator in the framework of marginal semiparametric model. Similarly to Zhu, Fung and He (2008), we give explicit expression for the asymptotic bias of regression spline estimator for nonparametric function f. Our results also show that the asymptotic bias of the regression spline estimator does not depend on the working covariance matrix, which distinguishes the regression splines from the smoothing splines and the seemingly unrelated kernel. To understand the local bias result of the regression spline estimator, we show that the regression spline estimator can be obtained iteratively by applying the standard weighted least squares regression spline estimator to pseudo-observations. At each iteration, the bias of the estimator is unchanged and only the variance is updated.

关键词： asymptotic bias B-spline generalized estimating equation longitudinal data semiparametric models 62F35 62G08

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The Balanceabililty of aMM Scheduling Policy for GSS ystems

The Balanceabililty of aMM Scheduling Policy for GSS ystems

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第二十九届中国控制会议

作者： WANG Xingxuan1,ZHENG Da-Zhong2 1 Department of Electronic Engineering,fudan university,Shanghai 200433,P.R.China2 Department of Automation,Tsinghua university,Beijing 100084,P.R.China

The generalized switched server（GSS）system and some switching scheduling policies,such as the FRR,FMM,aMM,and FSS policy,have been proposed for resolving a cluster of task balancing problems,which exist in a wide varietyof engineering *** paper mainly studies the balanceability of the aMM switching scheduling policy for the generalized switched server *** maximum load status difference（MLD）,called as the system difference,is *** is shown that the GSS system would have a smaller MLD under the aMM policy than under the FRR policy,and further,for a given GSS system,the aMM policy is balanceable if the FRR policy is.A general case where the number of the parallel units N is arbitrary is investigated,and a sufficient condition for the balanceability of the aMM policy in the case where the N equals to three is obtained.

关键词： Generalized Switched Server Systems(GSS) Switching Scheduling Policy Balanceability Load Balancing Hybrid Dynamic Systems(HDS) Algebra Constraint

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System Control Design for Generalized Switched Server Systems

System Control Design for Generalized Switched Server System...

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第二十九届中国控制会议

作者： WANG Xingxuan1,ZHENG Da-Zhong2 1.Department of Electronic Engineering,fudan university,Shanghai 200433,P.R.China 2.Department of Automation,Tsinghua university,Beijing 100084,P.R.China

The generalized switched server（GSS）systems model and some switching control strategies have been proposed to address a class of load balancing problems with system control input algebraic *** on the presented framework of the GSS systems and the related switching control strategies,this paper mainly studies the problem of the system control design for the GSS *** to that the GSS system is a discretely controlled continuous time system,the procedure of the control design is divided into two steps:one is to design the continuous time controller,and the other is to design and select the discrete-event driven switching control strategy.A numerical example is given to demonstrate the procedure of the system control design for the GSS systems.

关键词： System Control Design Generalized Switched Server Systems(GSS) Switching Scheduling Policy Load Balancing Hybrid Dynamic Systems(HDS) Algebraic Constraint

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