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Expression of the MAGE-1 gene in human hepatocellular carcinomas

Chinese Medical Journal 2000年第12期113卷 56-62页

作者：陈红松蔡胜利王瑜赵海涛彭吉润庞学文朱继业丛旭芮静安冷希圣杜如昱王宇陈慰峰 Hepatology Institute People's Hospital Peking University Beijing 100044 China Surgical Department People's Hospital Peking University Beijing 100044 China Immunology Department Liver Cancer Research Center Peking University Beijing 100083 China School of Basic Medical Science Liver Cancer Research Center Peking University Beijing 100083 China Ludwig Institute for Cancer Research Austin Campus Studley Road Heidelberg Vic. 3085 Australia Immunology Department 38 XueYuan Road Beijing 100083 China

To further investigate the expression of MAGE 1 gene in hepatocellular carcinoma (HCC) Methods The tumors and adjacent liver tissue from 45 HCC patients and liver tissue from 28 non HCC patients (16 with liver cirrhosis and 12 with normal liver) were characterized by RT PCR A 421?bp PCR product from a cDNA fragment spanning exons 1, 2 and 3 was sequenced The HLA type was assayed by standard ELISA in 43 HCC patients Results Thirty two of 45 tumor tissues from HCC patients expressed MAGE 1 mRNA (71 1%) In contrast, MAGE 1 mRNA was not detected in adjacent tissues Three were found to have point mutations at 3 identical sites resulting in the substitution of two amino acid residues The most frequent HLA types in 43 HCC patients were: HLA A2, 53 5%; A11, 25 6%; A24, 20 9%; A33, 20 9%; HLA B13, 28 3% and B35, 23 2% Expression of HLA A33 (20 9%) was higher in HCC patients than that predicted in the normal Chinese population (8 8%) There was no discernable correlation between MAGE 1 expression and α FP level, tumor size and hepatitis B or C virus infection The identification of peptides which are restricted by haploptypes other than A1 should increase the opportunity for peptide based immunotherapy Conclusions This study shows that MAGE 1 mRNA is highly expressed in HCC tumor tissue in Chinese patients Previously unreported point mutations in the MAGE 1 gene are described and may also provide additional opportunities for immunotherapy

关键词： hepatocellular carcinoma melanoma antigen

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