检索结果-南通市图书馆

Global Analysis of Expression Profiles of Rice Receptor-Like Kinase Genes

在线全文

学校读者我要写书评

暂无评论

Molecular Plant 2012年第1期5卷 143-153页

作者： Lin-Lin Gao Hong-Wei Xue National Key Laboratory of Plant Molecular Genetics Institute of Plant Physiology and EcologyShanghai Institutes for Biological Science(SIBS)ChineseAcademy of Sciences300 Fenglin Road20032 ShanghaiChina

The receptor-like kinases （RLKs） play critical roles in plant development and response to stress stimuli. By perceiving or sensing the extracellular signals, RLK activates the downstream signaling pathway through phosphorylating the specific targets. Up to now, only a few RLKs have been functionally identified, which are even fewer in rice （Oryza sativa L.）. We here report the systemic analysis of the expression profiles of rice RLK coding genes in different tissues, with the emphasis on seed development and in response to both abiotic stress and plant hormones. The results showed that most rice RLK genes are expressed in two or more tissues, of which the RLCK-RLKs have a higher, while WAK- and SD-RLKs have a lower, expression level in the vegetative tissues than other subfamily members. Interestingly, the constitutively highly expressed RLKs in rice and Arabidopsis are conserved, which is consistent with the previous hypothesis that RLKs existed before the differentiation of monocotyledon and dicotyledon plants. Nearly one-third of the detected rice RLKs are expressed during seed development, and the RLCK-RLK members possess a higher percentage during the endosperm de- velopment, suggesting a novel function of RLCK-RLK members in endosperm development. Further analysis revealed that many RLK genes expressed during seed development are also regulated by abiotic stresses （cold, salt, or drought） or hor- mones, indicating that RLKs may take part in the stress-related signaling pathways such as dehydration of endosperm. These results provide informative insights into the RLK studies and will be helpful to reveal the global regulatory network controlling rice seed development.

关键词： Receptor-like kinase rice profiling seed development stress ABA.

SPT6L Encoding a Putative WG/GW-Repeat Protein Regulates Apical-Basal Polarity of Embryo in Arabidopsis

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Molecular Plant 2012年第1期5卷 249-259页

作者： Xiao-Lu Gu Hua Wang Hai Huang Xiao-Feng Cui National Laboratory of Plant Molecular Genetics Shanghai Institute of Plant Physiology and EcologyShanghai Institutes for Biological SciencesChineseAcademy of Sciences300 Fenglin RoadShanghai 200032China

In eukaryotes, a protein motif consisting of WG/GW repeats, also called the Argonaute （AGO） hook, is thought to be essential for binding AGO proteins to fulfill their functions in RNA-mediated gene silencing. Although a number of WG/GW-containing proteins have been computationally identified in Arabidopsis, their roles in plant growth and development are unknown. Here, we show that the Arabidopsis Suppressor of Ty insertion 6-like （SPT6L） gene, which encodes a protein with C-terminal WG/GW repeats, plays critical roles in embryonic development. SPT6L is evolutionarily conserved only in vascular plants, with varying numbers of C-terminal WG/GW repeats, which are plant-species specific. spt61 mutants formed embryos with an aberrant apical-basal axis, showing insufficient development of the basal domain and embryonic lethality. Expression domains of the class-Ill homeodomain-leucine zipper （HD-ZIP III） genes PHABULOSA （PHB） and PHAVOLUTA （PHV） were expanded in the spt61 embryo. In contrast, the PLETHORA1 （PLT1） gene, which acts antagonistically to the HD-ZIP III genes in specification of basal fate, was severely down-regulated in the spt61 mutant. Furthermore, the phb phv double mutations partially rescued aberrant basal development in the spt61 background and restored PLT1 expression. Collectively, our results indicate that SPT6L is essential for specification of the apical-basal axis, partly by controlling the HD-ZIP III genes in embryos.

关键词： Arabidopsis embryo HD-ZIP ill polarity SPT6L.

Effects of CpG oligodeoxynucleotide on transcription factors GATA-3 and T-bet mRNA expression in asthmatic mice

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Acta Pharmacologica Sinica 2005年第9期26卷 1117-1122页

作者： Zu-qun WU~(1,2) Yi-ping XU~3 Hua XIANG~4 Hua-hao SHEN~11 Department of Respiratory Medicine,the Second Affiliated Hospital,4 Department of Pathology,the First Affiliated Hospital,School of Medicine,Zhejiang University,Hangzhou 310009,China 3 Department of Respiratory Medicine,Renji Hospital,shanghai Second Medical University shanghai 200001,China Department of Respiratory Medicine the Second Affiliated Hospital Hangzhou China Department of Respiratory Medicine Renji Hospital Shanghai Second Medical University Shanghai China Department of Pathology the First Affiliated Hospital School of Medicine Zhejiang University Hangzhou China

Aim:To investigate effects ofCpG oligod'eoxynucleotide(CpG ODN)on the mRNAexpression of transcription factors GATA binding protein 3(GATA-3)and T-boxexpressed in T cells(T-bet)in asthmatic ***:An asthmatic mousemodel was established and treated with CpG *** inflammatory cells andeosinophils in bronchoalveolar lavage fluid(BALF)were counted and inflamma-tory cell infiltration in lung tissue was ***-y and interleukin-4concentrations in BALF and splenocyte culture supernatants were detected us-ing an enzyme-linked immunosorbent *** factor GATA-3 and T-bet mRNA expression in splenocytes and lung tissue were detected by reversetranscription-polymerase chain ***:Total inflammatory cells andeosinophils in BALF were reduced in the CpG ODN-treated group compared withthe asthma group,and inflammatory cell infiltration in lung tissue was also signifi-cantly *** ODN treatment increased the interferon-γ concentrationbut decreased the interleukin-4 concentration in both BALF and splenocyte cul-ture ***-3 mRNA expression was reduced in both lung tissueand splenocytes in the CpG ODN-treated group,while the mRNA ratio ofT-bet toGATA-3 in splenocytes was ***:CpG ODN treatment inhibitsairway inflammatory cell infiltration and regulates interferon-y/interleukin-4 syn-thesis in asthmatic mice,possibly through a mechanism of downregulation ofGATA-3 mRNA expression in both lung tissue and splenocytes.

关键词： asthma inflammation CpG oligodeoxy-nucleotide GATA-3 T-bet mice

Biomarkers of neurodegenerative disorders: How good are they?

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Cell Research 2004年第5期14卷 347-358页

作者： Varun RACHAKONDA Department of Neurology Baylor College of Medicine Houston TX USA

Biomarkers are very important indicators of normal and abnormal biological processes. Specific changes in pathologies, biochemistries and genetics can give us comprehensive information regarding the nature of any particular disease. A good biomarker should be precise and reliable, distinguishable between normal and interested disease, and differential between different diseases. It is believed that biomarkers have great potential in predicting chances for diseases, aiding in early diagnosis, and setting standards for the development of new remedies to treat diseases. New technologies have enabled scientists to identify biomarkers of several different neurodegenerative diseases. The followings, for instance, are only a few of the many new biomarkers that have been recently identified: the phosphorylated tau protein and aggregated β-amyloid peptide for Alzheimer’s disease (AD), α-synuclein contained Lewy bodies and altered dopamine transporter (DAT) imaging for Parkinson’s disease (PD), SOD mutations for familial amyotrophic lateral sclerosis (ALS), and CAG repeats resulted from Huntington’s gene mutations in Huntington’s disease (HD). This article will focus on the most-recent findings of biomarkers belonging to the four mentioned neurodegenerative diseases.

关键词： Alzheimer’s disease Parkinson’s disease Amyotrophic Lateral Sclerosis Huntington’s disease biomarkers.

Extra-copy nifA enhances the nodulation efficiency of Sinorhizobium fredii

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Chinese Science Bulletin 2002年第7期47卷 565-567页

作者： Zhao Jieping Dai Xiaomi Xu Ling Zhu Jiabi Shen Shanjiong Yu Guanqiao Laboratory of Molecular Genetics Shanghai Institute of Plant Physiology and EcologyShanghai Institutes of Biological SciencesChinese Academy of Sciences200032ShanghaiChina

Previous investigations have shown that nifA gene is involved in nodulation and symbiotic nitrogen fixation regulation of Rhizobium. We study the role of nifA on nodulation of leguminous plants. We found that Sinorhizo-bium fredii harboring multi-copy plasmid carrying the con-stitutively expressed Klebsiella pneumoniae nifA exhibited an increase of nodulation activity and nodulation competitiveness on soybean plants. The Nod-factor secreted by the rhizobia cells containing the multi-copied nifA was assayed, and preliminary results showed that S. fredii containing the multi-copy plasmid carrying nifA produced higher strength of Nod-factor than the rhizobia containing the same plasmid carrying the vector did.

关键词： Sinorhizobium fredii nifA Nod-factor nodulation com-petitiveness.

Heat shock response down-regulates IL-18 expression inthe murine macrophage cell line, RAW264.7

在线全文

学校读者我要写书评

暂无评论

Chinese Science Bulletin 2003年第8期48卷 780-785页

作者： WANG Yun LI Changlin WANG Xingyu ZHANG Jinsong ZHANG Zongliang Department of Cell Biology The Third Military Medical UniversityChongqing 400038China shanghai Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological SciencesChinese Academy of SciencesShanghai 200031China

Heat shock response is a self-defense mecha-nism for protection of cells and organisms from a wide range of harmful stressors. Recent studies revealed that it is in-volved in the regulation of cytokines expression. IL-18 is an important cytokine in mediating immune response. We stud-ied LPS-induced IL-18 expression in heat shock treated RAW264.7 murine macrophages. Our results show that the heat shock response significantly inhibited the expression of LPS-induced pro-inflammatory cytokine IL-18. Further research on the down-regulation mechanism shows that this inhibitory effect is correlated to the great suppression of the binding activity of AP-1, which is a transcription factor binding to the promoter of IL-18 (-1120 to -1083) and regu-lates the transcription of IL-18. Meanwhile, we observed that the phosphorylation of JNK, which is AP-1 upstream kinase, was greatly decreased. These results confirmed that the down-regulation effect on IL-18 production in heat shock response is related to the suppression of the JNK/AP-1 sig-naling pathway.

关键词：热震反应巨噬细胞自卫机制 HSR 环境压力

Proteomic and bioinformatic analyses of possible targetrelated proteins of gambogic acid in human breast carcinoma MDA-MB-231 cells

在线全文

学校读者我要写书评

暂无评论

Chinese Journal of Natural Medicines 2015年第1期13卷 41-51页

作者： LI Dong SONG Xiao-Yi Yue Qing-Xi CUI Ya-Jun LIU Miao FENG Li-Xing WU Wan-Ying JIANG Bao-Hong YANG Min QU Xiao-Bo LIU Xuan GUO De-An Changchun University of Chinese Medicine shanghai Institute of Materia Medica Chinese Academy of Sciences shanghai University of Traditional Chinese Medicine

Gambogic acid(GA) is an anticancer agent in phase Ⅱb clinical trial in China but its mechanism of action has not been fully clarified. The present study was designed to search the possible target-related proteins of GA in cancer cells using proteomic method and establish possible network using bioinformatic analysis. Cytotoxicity and anti-migration effects of GA in MDA-MB-231 cells were checked using MTT assay, flow cytometry, wound migration assay, and chamber migration assay. Possible target-related proteins of GA at early(3 h) and late stage(24 h) of treatment were searched using a proteomic technology, two-dimensional electrophoresis(2-DE). The possible network of GA was established using bioinformatic analysis. The intracellular expression levels of vimentin, keratin 18, and calumenin were determined using Western blotting. GA inhibited cell proliferation and induced cell cycle arrest at G2/M phase and apoptosis in MDA-MB-231 cells. Additionally, GA exhibited anti-migration effects at non-toxic doses. In 2-DE analysis, totally 23 possible GA targeted proteins were found, including those with functions in cytoskeleton and transport, regulation of redox state, metabolism, ubiquitin-proteasome system, transcription and translation, protein transport and modification, and cytokine. Network analysis of these proteins suggested that cytoskeleton-related proteins might play important roles in the effects of GA. Results of Western blotting confirmed the cleavage of vimentin, increase in keratin 18, and decrease in calumenin levels in GA-treated cells. In summary, GA is a multi-target compound and its anti-cancer effects may be based on several target-related proteins such as cytoskeleton-related proteins.

关键词：传统医药药理学网络中国药物发现生物制药生物医学生物系统

FOXP3 ＋ regulatory T cells and their functional regulation

在线全文

学校读者我要写书评

暂无评论

Cellular & Molecular Immunology 2015年第5期12卷 558-565页

作者： Zhiyuan Li Dan Li Andy Tsun Bin Li Unit of Molecular Immunology Key Laboratory of Molecular Virology & Immunology Institut Pasteur of Shanghai Chinese Academy of Sciences Shanghai China Innovent Biologics Inc Suzhou China

FOXP3＋ regulatory T （Treg） cells are critical in maintaining immune tolerance and homeostasis of the immune system. The molecular mechanisms underlying the stability, plasticity and functional activity of Treg cells have been much studied in recent years. Here, we summarize these intriguing findings, and provide insight into their potential use or manipulation during Treg cell therapy for the treatment of autoimmune diseases, graft-versus-host disease （GVHD） and cancer.

关键词： autoimmune diseases FOXP3 immunosuppressive activity Treg cells

Comparison of gut-associated and nest-associated microbial communities of a fungus-growing termite （Odontotermes yunnanensis）

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Insect Science 2010年第3期17卷 265-276页

作者： Yan-Hua Long Lei Xie Ning Liu Xing Yan Ming-Hui Li Mei-Zhen Fan Qian Wang Provincial Key Laboratory of Microbial Pest Control Anhui Agricultural University Hefei Institute of Plant Physiology and Ecology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai China

The digestion of cellulose by fungus-growing termites involves a complex of different organisms, such as the termites themselves, fungi and bacteria. To further investigate the symbiotic relationships of fungus-growing termites, the microbial communities of the termite gut and fungus combs of Odontotermes yunnanensis were examined. The major fungus species was identified as Termitomyces sp. To compare the micro-organism diversity between the digestive tract of termites and fungus combs, four polymerase chain reaction clone libraries were created （two fungus-targeted internal transcribed spacer [ITS] - ribosomal DNA [rDNA] libraries and two bacteria-targeted 16S rDNA libraries）, and one library of each type was produced for the host termite gut and the symbiotic fimgus comb. Results of the fungal clone libraries revealed that only Termitomyces sp. was detected on the fungus comb; no non-Termitomyces fungi were detected. Meanwhile, the same fungus was also found in the termite gut. The bacterial clone libraries showed higher numbers and greater diversity of bacteria in the termite gut than in the fungus comb. Both bacterial clone libraries from the insect gut included Firmicutes, Bacteroidetes, Proteobacteria, Spirochaetes, Nitrospira, Deferribacteres, and Fibrobacteres, whereas the bacterial clone libraries from the fungal comb only contained Firmicutes, Bacteroidetes, Proteobacteria, and Acidobacteris.

关键词： fungus comb gut ITS phylotype termite Termitomyces