检索结果-南通市图书馆

Haplotype-resolved T2T genome assemblies and pangenome graph of pear reveal diverse patterns of allele-specific expression and the genomic basis of fruit quality traits

引用

Plant Communications 2024年第10期5卷 100-120页

作者： Qionghou Li Xin Qiao Lanqing Li Chao Gu Hao Yin Kaijie Qi Zhihua Xie Sheng Yang Qifeng Zhao Zewen Wang Yuhang Yang Jiahui Pan Hongxiang Li Jie Wang Chao Wang Loren H.Rieseberg Shaoling Zhang Shutian Tao National Key Laboratory of Crop Genetics and Germplasm Enhancement and Utilization Sanya Institute of Nanjing Agricultural UniversityCollege of HorticultureNanjing Agricultural UniversityNanjingJiangsu 210095China Pomology Institute Shanxi Agricultural UniversityTaiguShanxi 030801China Department of Botany and Biodiversity Research Centre University of British ColumbiaVancouverBCCanada

Hybrid crops often exhibit increased yield and greater resilience,yet the genomic mechanism(s)underlying hybrid vigor or heterosis remain unclear,hindering our ability to predict the expression of phenotypic traits in hybrid ***,we generated haplotype-resolved T2T genome assemblies of two pear hybrid varieties,‘Yuluxiang’(YLX)and‘Hongxiangsu’(HXS),which share the same maternal parent but differ in their paternal *** then used these assemblies to explore the genome-scale landscape of allele-specific expression(ASE)and create a pangenome graph for *** was observed for close to 6000 genes in both hybrid cultivars.A subset of ASE genes related to aspects of fruit quality such as sugars,organic acids,and cuticular wax were identified,suggesting their important contributions to ***,Ma1,a gene regulating fruit acidity,is absent in the paternal haplotypes of HXS and YLX.A pangenome graph was built based on our assemblies and seven published pear *** data for 139 cultivated pear genotypes(including 97 genotypes sequenced here)were subsequently aligned to the pangenome graph,revealing numerous structural variant hotspots and selective sweeps during pear diversifica*** predicted,the Ma1 allele was found to be absent in varieties with low organic acid content,and this association was functionally validated by Ma1 overexpression in pear fruit and ***,these results reveal the contributions of ASE to fruit-quality heterosis and provide a robust pangenome reference for high-resolution allele discovery and association mapping.

关键词： pear haplotype-resolved assembly allele-specific expression fruit quality traits pangenome graph

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Deletion of a 1,049 bp sequence from the 5′UTR upstream of the SiHEC3 gene induces a seed non-shattering mutation in sesame

引用

Journal of Integrative Agriculture 2024年第8期23卷 2589-2604页

作者： Ming Ju Guiting Li Qiuzhen Tian Hengchun Cao Qin Ma Yinghui Duan Hui Guo Zhanyou Zhang Yingying Huang Huili Wang Haiyang Zhang Hongmei Miao The Shennong Laboratory Zhengzhou 450002China Henan Sesame Research Center Henan Academy of Agricultural SciencesZhengzhou 450002China Key Laboratory of Specific Oilseed Crops Genomics of Henan Province Henan Sesame Research CenterHenan Academy of Agricultural SciencesZhengzhou 450002China

Sesame is a labor intensive crop with limited mechanized harvesting mainly due to the seed shattering(SS)*** this study,we performed a genetic analysis of the seed-shattering resistance(SR)trait with a SR sesame mutant *** the SS type,the parenchyma cells in the abscission zone of the 12M07 mutant are arranged loosely but adhere to the seed *** analysis of six generations derived from 12M07(SR)×Xiangcheng Dazibai(SS)showed that the SR trait is recessive and controlled by a single gene *** mapping of the F2population with 888,619 variants(single-nucleotide polymorphisms(SNPs)and insertion-deletion(InDels))and 31,884 structural variations(SVs)determined that only SV12002 in the 5′upstream region of gene Sindi0765000(named SiHEC3)in Chr.3 was significantly associated with the SR ***3 encodes the bHLH transcription factor.A 1,049 bp deletion occurred in the 5′UTR of Sihec3 in ***3 is mainly expressed in developing placental tissues,with the expression peaking in capsules at 45 days after pollination.A dual-luciferase reporter assay in tobacco confirmed that the promoter activity of Sihec3 was reduced because of the deletion of the 1,049 bp promoter ***–protein interaction network analysis showed that HEC3 is co-expressed with nine key proteins,such as SHATTERPROOF1(SHP1)and SEEDSTICK(STK)which participate in the secondary wall biosynthesis of the abscission layer in *** findings of this study show the important function of Sihec3corresponding with the SR trait and supply the genetic information for breeding new varieties that are amenable to mechanized harvesting in sesame and other crops.

关键词： sesame(Sesamum indicum L.) association mapping seed shattering structure variation bHLH transcription factor

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Straw interlayer improves sunflower root growth: Evidence from moisture and salt migration and the microbial community in saline-alkali soil

引用

Journal of Integrative Agriculture 2024年第11期23卷 3870-3881页

作者： Mengmeng Chen Guoli Wang Yupeng Jing Jie Zhou Jiashen Song Fangdi Chang Ru Yu Jing Wang Weini Wang Xia Sun Hongyuan Zhang Yuyi Li State Key Laboratory of Efficient Utilization of Arid and Semi-arid Arable Land in Northern China Institute of Agricultural Resources and Regional PlanningChinese Academy of Agricultural Sciences Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences College of Agriculture Nanjing Agricultural University Ordos Agriculture and Animal Husbandry Ecology and Resource Protection Center

A straw interlayer added to soil can effectively reduce soil salinity effects on plant growth, however, the effects of soil moisture, salt and microbial community composition on plant growth under a straw interlayer are unclear. A rhizobox study was conducted to investigate the role of straw interlayer thickness on soil moisture, salt migration, microbial community composition, as well as root growth in sunflower. The study included four treatments: Control(no straw interlayer); S3(straw interlayer of 3.0 cm); S5(straw interlayer of 5.0 cm); S7(straw interlayer of 7.0 cm). Straw interlayer treatments increased soil moisture by 8.2–11.0% after irrigation and decreased soil salt content after the bud stage in 0–40 cm soil. Total root length, total root surface area, average root diameter, total root volume and the number of root tips of sunflower plants were higher under straw interlayer treatments than in the control, and were the highest under the S5 treatment. This stimulated root growth was ascribed to the higher abundance of Chloroflexi and Verrucomicrobia bacteria in soil with a straw interlayer, which was increased by 55.7 and 54.7%, respectively,in the S5 treatment. Addition of a straw interlayer of 5 cm thickness is a practical and environmentally feasible approach for improving sunflower root growth in saline-alkali soil.

关键词： saline-alkali soil rhizobox soil water soil salt content bacterial community

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

KIF5B-mediated internalization of FMDV promotes virus infection

引用

Virologica Sinica 2024年第3期39卷 378-389页

作者： Wei Zhang Fan Yang Yang Yang Weijun Cao Wenhua Shao Jiali Wang Mengyao Huang Zhitong Chen Xiaoyi Zhao Weiwei Li Zixiang Zhu Haixue Zheng State Key Laboratory for Animal Disease Control and Prevention College of Veterinary MedicineLanzhou UniversityLanzhou Veterinary Research InstituteChinese Academy of Agricultural SciencesLanzhou 730000China Gansu Province Research Center for Basic Disciplines of Pathogen Biology Lanzhou 730046China

Foot-and-mouth disease(FMD)is a highly contagious and economically important disease,which is caused by the FMD virus(FMDV).Although the cell receptor for FMDV has been identified,the specific mechanism of FMDV internalization after infection remains *** this study,we found that kinesin family member 5B(KIF5B)plays a vital role during FMDV ***,we confirmed the interaction between KIF5B and FMDV structural protein VP1 by co-immunoprecipitation(Co-IP)and co-localization in FMDV-infected *** particular,the stalk[amino acids(aa)413–678]domain of KIF5B was indispensable for KIF5B-VP1 ***,overexpression of KIF5B dramatically enhanced FMDV replication;consistently,knockdown or knockout of KIF5B suppressed FMDV ***,we also demonstrated that KIF5B promotes the internalization of FMDV via regulating clathrin ***5B also promotes the transmission of viral particles to early and late endosomes during the early stages of *** conclusion,our results demonstrate that KIF5B promotes the internalization of FMDV via regulating clathrin uncoating and intracellular *** study may provide a new therapeutic target for developing FMDV antiviral drugs.

关键词： FMDV VP1 protein KIF5B Endosome Clathrin

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Insights into genetic diversity and phenotypic variations in domestic geese through comprehensive population and pan-genome analysis

引用

Journal of Animal Science and Biotechnology 2024年第1期15卷 88-107页

作者： Guangliang Gao Hongmei Zhang Jiangping Ni Xianzhi Zhao Keshan Zhang Jian Wang Xiangdong Kong Qigui Wang Chongqing Academy of Animal Science Rongchang DistrictChongqing 402460China Livestock and Poultry Multi‑Omics Key Laboratory of Ministry of Agriculture and Rural Affairs College of Animal Science and TechnologySichuan Agricultural UniversityChengdu 611130China Chongqing Engineering Research Center of Goose Genetic Improvement Rongchang DistrictChongqing 402460China Department of Cardiovascular Ultrasound and Non‑Invasive Cardiology Sichuan Academy of Medical Sciences and Sichuan Provincial People’s HospitalUniversity of Electronic Science and Technology of ChinaChengdu611731China Ultrasound in Cardiac Electrophysiology and Biomechanics Key Laboratory of Sichuan Province University of Electronic Science and Technology of ChinaChengdu611731China JiguangGene Biotechnology Co. Ltd.Nanjing210032China Jiangsu Agri-Animal Vocational College Taizhou225300China Poultry Science Institute Chongqing Academy of Animal ScienceNo.51 Changzhou AvenueRongchang DistrictChongqing 402460P.R.China

Background Domestic goose breeds are descended from either the Swan goose(Anser cygnoides)or the Greylag goose(Anser anser),exhibiting variations in body size,reproductive performance,egg production,feather color,and other phenotypic *** a pan-genome facilitates a thorough identification of genetic variations,thereby deepening our comprehension of the molecular mechanisms underlying genetic diversity and phenotypic *** To comprehensively facilitate population genomic and pan-genomic analyses in geese,we embarked on the task of 659 geese whole genome resequencing data and compiling a database of 155 RNA-seq *** constructing the pan-genome for geese,we generated non-reference contigs totaling 612 Mb,unveiling a collection of 2,813 novel genes and pinpointing 15,567 core genes,1,324 softcore genes,2,734 shell genes,and 878 cloud genes in goose ***,we detected an 81.97 Mb genomic region showing signs of genome selection,encompassing the TGFBR2 gene correlated with variations in body weight among ***-wide association studies utilizing single nucleotide polymorphisms(SNPs)and presence-absence variation revealed significant genomic associations with various goose meat quality,reproductive,and body composition *** instance,a gene encoding the SVEP1 protein was linked to carcass oblique length,and a distinct gene-CDS haplotype of the SVEP1 gene exhibited an association with carcass oblique ***,the pan-genome analysis revealed enrichment of variable genes in the“hair follicle maturation”Gene Ontology term,potentially linked to the selection of feather-related traits in geese.A gene presence-absence variation analysis suggested a reduced frequency of genes associated with“regulation of heart contraction”in domesticated geese compared to their wild *** study provided novel insights into gene expression features and functions by integrating gene expression patterns across multiple orga

关键词： Gene-CDS haplotype Goose GWAS Pan-genome Presence-absence variation Selection signal

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Predicting Lactobacillus delbrueckii subsp. bulgaricusStreptococcus thermophilus interactions based on a highly accurate semi-supervised learning method

引用

Science China Life Sciences 2024年

作者： Shujuan Yang Mei Bai Weichi Liu Weicheng Li Zhi Zhong Lai-Yu Kwok Gaifang Dong Zhihong Sun Key Laboratory of Dairy Biotechnology and Engineering Ministry of Education Inner Mongolia Agricultural University Key Laboratory of Dairy Products Processing Ministry of Agriculture and Rural Affairs Inner Mongolia Agricultural University Inner Mongolia Key Laboratory of Dairy Biotechnology and Engineering Inner Mongolia Agricultural University Collaborative Innovative Center for Lactic Acid Bacteria and Fermented Dairy Products Ministry of Education Inner Mongolia Agricultural University College of Computer and Information Engineering Inner Mongolia Agricultural University Inner Mongolia Autonomous Region Key Laboratory of Big Data Research and Application of Agriculture and Animal Husbandry

Lactobacillus delbrueckii subsp. bulgaricus(L. bulgaricus) and Streptococcus thermophilus(S. thermophilus) are commonly used starters in milk fermentation. Fermentation experiments revealed that L. bulgaricus-S. thermophilus interactions(LbSt I) substantially impact dairy product quality and production. Traditional biological humidity experiments are time-consuming and labor-intensive in screening interaction combinations, an artificial intelligence-based method for screening interactive starter combinations is necessary. However,in the current research on artificial intelligence based interaction prediction in the field of bioinformatics, most successful models adopt supervised learning methods, and there is a lack of research on interaction prediction with only a small number of labeled ***, this study aimed to develop a semi-supervised learning framework for predicting LbSt I using genomic data from 362 isolates(181per species). The framework consisted of a two-part model: a co-clustering prediction model(based on the Kyoto Encyclopedia of Genes and Genomes(KEGG) dataset) and a Laplacian regularized least squares prediction model(based on K-mer analysis and gene composition of all isolates datasets). To enhance accuracy, we integrated the separate outcomes produced by each component of the two-part model to generate the ultimate LbSt I prediction results, which were verified through milk fermentation experiments. Validation through milk fermentation experiments confirmed a high precision rate of 85%(17/20; validated with 20 randomly selected combinations of expected interacting isolates). Our data suggest that the biosynthetic pathways of cysteine, riboflavin, teichoic acid, and exopolysaccharides, as well as the ATP-binding cassette transport systems, contribute to the mutualistic relationship between these starter bacteria during milk fermentation. However, this finding requires further experimental verification. The presented model and data are valua

关键词：

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

利用纳米气泡控制农业废水灌溉系统复合污垢的机制

引用

Engineering 2024年第4期35卷 214-225页

作者： Yang Xiao Bo Zhou Siyuan Tan Lei Li Tahir Muhammad Buchun Si Changjian Ma Sunny C.Jiang Yunkai Li College of Water Resources and Civil Engineering China Agricultural UniversityBeijing 100083China Engineering Research Center for Agricultural Water-Saving and Water Resources(Ministry of Education of the People’s Republic of China) China Agricultural UniversityBeijing 100083China School of Environment and Civil Engineering Dongguan University of TechnologyDongguan 523808China State Key Laboratory of Nutrient Use and Management Institute of Agricultural Resources and EnvironmentShandong Academy of Agricultural SciencesJinan 250100China Department of Civil and Environmental Engineering University of California-IrvineIrvineCA 92617USA

The increasing demand for wastewater treatment has become a notable trend for addressing global water ***,fouling is a significant challenge for wastewater distribution engineering *** study provides an approach using nanobubbles(NBs)to control *** antifouling capacities of three types of NBs,six oxygen concentrations,and two application procedures(prevention and removal)are *** results show that NBs effectively mitigate composite fouling—including biofouling,inorganic scaling,and particulate fouling—in comparison with the non-NBs *** specifically,hydroxyl radicals generated by the self-collapse of NBs oxidize organics and kill microorganisms in *** negatively charged surfaces of the NBs transform the crystalline form of CaCO_(3)from calcite to looser aragonite,which reduces the likelihood of ion ***,the NBs gas-liquid interfaces act as gas"bridges"between colloidal particles,enhancing the removal of particles from ***,although the NBs inhibit the growth of fouling,they do not significantly remove the already adhered fouling in non-NBs treated *** study anticipates that the application of NBs will address the significant fouling issue for various wastewater distribution engineering systems in order to meet the global challenge of sustainable water supplies.

关键词： Biofouling Scaling Colloidal fouling Wastewater Antifouling mechanism

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Effects of mulch and planting methods on Medicago ruthenica seed yield and soil physical-chemical properties

引用

Journal of Arid Land 2022年第8期14卷 894-909页

作者： WANG Yuxia ZHANG Jing YU Xiaojun College of Grassland Science Gansu Agricultural UniversityLanzhou 730070China

Medicago ruthenica (L.) Trautv., a wild grass species, is commonly grown as a forage crop in arid and semi-arid areas of China. Herein, we explored mulch patterns and planting methods for optimizing M. ruthenica seed production in the loess plateau of the Gansu Province, China from 2017 to 2019. The experiments comprised of six treatments including (1) flat ground without mulch (F0, control);(2) flat ground with a transparent white 0.008 mm thick plastic film mulch (FP);(3) flat ground with 4500 kg/hm2 straw mulch (FS);(4) furrow with 10 cm ridges (R0);(5) furrow with plastic film mulch (RP);and (6) furrow with straw mulch (RS). Results showed that the harvested seed yield of M. ruthenica was the highest under RP treatment, followed by FP and FS treatments. Soil moisture content from mid-May to mid-August in 2017 was the highest under RP and FP treatments, followed by RS and FS treatments. In 2018, soil moisture content was the highest under RS and FS treatments. In 2017 and 2018, soil temperature was the highest under FP and RP treatments, followed by F0 and R0 treatments. Total and available nitrogen, phosphorus, and potassium contents were the highest under RS and FS treatments, followed by RP and FP treatments. Comprehensive analysis result showed that surface mulch improved soil microenvironment and increased seed yield of M. ruthenica. Straw mulch also effectively recycled excess crop straw, thereby encouraging the sustainable development of agriculture in this area. In conclusion, FS treatment was considered the best mode for M. ruthenica seed production in this area.

关键词： arid area plastic film straw mulch soil moisture content soil temperature soil chemistry

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

解锁作物多样性:通过基因组编辑增加遗传变异（英文）

引用

Science Bulletin 2024年第3期 281-284页

作者：邹金鹏黄勇高彩霞王克剑 State Key Laboratory of Rice Biology and Breeding China National Rice Research Institute Chinese Academy of Agricultural Sciences New Cornerstone Science Laboratory Center for Genome Editing Institute of Genetics and Developmental Biology Chinese Academy of Sciences College of Advanced Agricultural Sciences University of Chinese Academy of Sciences Hainan Yazhou Bay Seed Laboratory

The germplasm resource repository harbors an extensive collection of genetic variations,providing a crucial foundation for the survival and sustainable development of *** history,major agricultural breakthroughs have relied on safeguarding,exploring,and harnessing germplasm ***,the pursuit of high yields in modern agriculture has led to a continuous reduction in biodiversity,resulting in monocultures and an undesirable homogeneity of breeding *** a consequence,germplasm resources are facing the alarming prospect of accelerated loss leading to a decline in crop ***,

关键词：

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Fine-mapping and transcriptome analysis of the photosensitive leaf-yellowing gene CaLY1 in pepper(Capsicum annuum L.)

引用

Horticultural Plant Journal 2023年第1期9卷 122-132页

作者： Sha Yang Zhuqing Zhang Wenchao Chen Chengliang Liang Xuefeng Li Zhoubin Liu Qingzhi Cui Yanqing Ma Xuexiao Zou College of Horticulture Hunan Agricultural UniversityChangsha 410128China Vegetable Institution of Hunan Academy of Agricultural Science Changsha 410125China

Leaf color is directly related to altered ***,leaf yellowing mutants can be widely used for the researching plant physiology and functional genomes,for cultivating new varieties of popular horticultural plants,and for identifying hybrid purity(as markers).Here,we constructed a^(60)Co-γF_(2)population from the leaf-yellowing mutant R24 via radiation mutation with the inbred line WT21 of *** analysis showed that the leaf-yellowing of the mutant was controlled by a single recessive *** applying the Bulk Segregation Analysis and Kompetitive Allele Specific PCR markers,the leaf-yellowing gene CaLY1(Capsicum annuum Leaf yellow 1)was mapped on chromosome 9,SNP5791587-SNP6011215,with a size of 214.5 *** non-synonymous mutated gene Capana09g000166 was found in the *** gene encoded a Psb X,which is the core complex of PSⅡ.Transcriptome analysis further showed that 2301 differentially expressed genes were identified under shading treatment for 24 h in *** Gene Ontology enrichment pathways were related to photosynthesis light harvesting,cell wall,activity of quercetin 3-O-glucosyltransferase and flavonoid metabolic process,which likely regulate the response of pepper leaves to different light *** enrichment analysis indicated that the most abundant pathways were photosynthesis antenna proteins and metabolic.

关键词： Pepper Leaf-color yellow Fine-mapping Gene Photosensitive

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：