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Identification of residues in Lassa virus glycoprotein 1 involved in receptor switch

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Virologica Sinica 2024年第4期39卷 600-608页

作者： Jiao Guo Yi Wan Yang Liu Xiaoying Jia Siqi Dong Gengfu Xiao Wei Wang State Key Laboratory of Virology Wuhan Institute of VirologyCenter for Biosafety Mega-ScienceChinese Academy of SciencesWuhan 430071China University of the Chinese Academy of Sciences Beijing 100049China The Xi'an Key Laboratory of Pathogenic Microorganism and Tumor Immunity School of Basic MedicineXi'an Medical UniversityXi'an 710021China

Lassa virus(LASV)is an enveloped,negative-sense RNA virus that causes Lassa hemorrhagic *** entry of LASV requires the viral glycoprotein 1(GP1)to undergo a receptor switch from its primary receptor alpha-dystroglycan(α-DG)to its endosomal receptor lysosome-associated membrane protein 1(LAMP1).A conserved histidine triad in LASV GP1 has been reported to be responsible for receptor *** test the hypothesis that other non-conserved residues also contribute to receptor switch,we constructed a series of mutant LASV GP1 proteins and tested them for binding to *** residues,L84,K88,L107,and H170,were identified as critical for receptor *** any of the four residues with the corresponding lymphocytic choriomeningitis virus(LCMV)residue(L84 N,K88E,L10F,and H170S)reduced the binding affinity of LASV GP1 for ***,all mutations caused decreases in glycoprotein precursor(GPC)-mediated membrane fusion at both pH 4.5 and *** infectivity of pseudotyped viruses bearing either GPCL84N or GPCK88E decreased sharply in multiple cell types,while L107F and H170S had only mild effects on *** biolayer light interferometry assay,we found that all four mutants had decreased binding affinity to LAMP1,in the order of binding affinity being L84 N>L107F>K88E>*** four amino acid loci identified for the first time in this study have important reference significance for the in-depth investigation of the mechanism of receptor switching and immune escape of LASV occurrence and the development of reserve anti-LASV infection drugs.

关键词： Lassa virus(LASV) Lysosome-associated membrane protein 1 (LAMP1) Glycoprotein Receptor switch Membrane fusion

Blockchain-Based Key Management Scheme Using Rational Secret Sharing

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Computers, Materials & Continua 2024年第4期79卷 307-328页

作者： Xingfan Zhao Changgen Peng Weijie Tan Kun Niu State Key Laboratory of Public Big Data College of Computer Science and TechnologyGuizhou UniversityGuiyang550025China Guizhou Big Data Academy Guizhou UniversityGuiyang550025China

Traditional blockchain key management schemes store private keys in the same location,which can easily lead to security issues such as a single point of ***,decentralized threshold key management schemes have become a research focus for blockchain private key *** security of private keys for blockchain user wallet is highly related to user identity authentication and digital asset *** threshold blockchain private key management schemes based on verifiable secret sharing have made some progress,but these schemes do not consider participants’self-interested behavior,and require trusted nodes to keep private key fragments,resulting in a narrow application scope and low deployment efficiency,which cannot meet the needs of personal wallet private key escrow and recovery in public *** design a private key management scheme based on rational secret sharing that considers the self-interest of participants in secret sharing protocols,and constrains the behavior of rational participants through reasonable mechanism design,making it more suitable in distributed scenarios such as the public *** proposed scheme achieves the escrow and recovery of personal wallet private keys without the participation of trusted nodes,and simulate its implementation on smart *** to other existing threshold wallet solutions and keymanagement schemes based on password-protected secret sharing(PPSS),the proposed scheme has a wide range of applications,verifiable private key recovery,low communication overhead,higher computational efficiency when users perform one-time multi-key escrow,no need for trusted nodes,and personal rational constraints and anti-collusion attack capabilities.

关键词： Blockchain smart contract rational secret sharing key management

Exosomes derived from microglia overexpressing miR-124-3p alleviate neuronal endoplasmic reticulum stress damage after repetitive mild traumatic brain injury

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Neural Regeneration research 2024年第9期19卷 2010-2018页

作者： Yan Wang Dai Li Lan Zhang Zhenyu Yin Zhaoli Han Xintong Ge Meimei Li Jing Zhao Shishuang Zhang Yan Zuo Xiangyang Xiong Han Gao Qiang Liu Fanglian Chen Ping Lei Department of Geriatrics Tianjin Medical University General HospitalTianjinChina Tianjin Geriatrics Institute Tianjin Medical University General HospitalTianjinChina Tianjin Neurological Institute Tianjin Medical University General HospitalTianjinChina

We previously reported that miR-124-3p is markedly upregulated in microglia-derived exosomes following repetitive mild traumatic brain ***,its impact on neuronal endoplasmic reticulum stress following repetitive mild traumatic brain injury remains *** this study,we first used an HT22 scratch injury model to mimic traumatic brain injury,then co-cultured the HT22 cells with BV2 microglia expressing high levels of *** found that exosomes containing high levels of miR-124-3p attenuated apoptosis and endoplasmic reticulum ***,luciferase reporter assay analysis confirmed that miR-124-3p bound specifically to the endoplasmic reticulum stress-related protein IRE1α,while an IRE1αfunctional salvage experiment confirmed that miR-124-3p targeted IRE1αand reduced its expression,thereby inhibiting endoplasmic reticulum stress in injured ***,we delivered microglia-derived exosomes containing miR-124-3p intranasally to a mouse model of repetitive mild traumatic brain injury and found that endoplasmic reticulum stress and apoptosis levels in hippocampal neurons were significantly *** findings suggest that,after repetitive mild traumatic brain injury,miR-124-3 can be transferred from microglia-derived exosomes to injured neurons,where it exerts a neuroprotective effect by inhibiting endoplasmic reticulum ***,microglia-derived exosomes containing miR-124-3p may represent a novel therapeutic strategy for repetitive mild traumatic brain injury.

关键词： apoptosis C/EBP homologous protein endoplasmic reticulum stress exosome inositol-requiring enzyme 1α microglia miR-124-3p neuron repetitive mild traumatic brain injury X-box binding protein 1

A Novel Anti-Collision Algorithm for Large Scale of UHF RFID Tags Access Systems

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Computers, Materials & Continua 2024年第7期80卷 897-912页

作者： Xu Zhang Yi He Haiwen Yi Yulu Zhang Yuan Li Shuai Ma Gui Li Zhiyuan Zhao Yue Liu Junyang Liu Guangjun Wen Jian Li School of Information and Communication Engineering/Yibin Institute University of Electronic Science and Technology of ChinaChengdu611731China Department of IoT Technology research China Mobile Research InstituteBeijing100053China The 10th research Institute of China Electronics Technology Group Corporation Chengdu611731China College of Information and Communication National University of Defense TechnologyWuhan430019China

When the radio frequency identification(RFID)system inventories multiple tags,the recognition rate will be seriously affected due to *** on the existing dynamic frame slotted Aloha(DFSA)algorithm,a sub-frame observation and cyclic redundancy check(CRC)grouping combined dynamic framed slotted Aloha(SUBF-CGDFSA)algorithm is *** algorithm combines the precise estimation method of the quantity of large-scale tags,the large-scale tags grouping mechanism based on CRC pseudo-randomcharacteristics,and the Aloha anti-collision optimization mechanism based on sub-frame *** grouping tags and sequentially identifying themwithin subframes,it accurately estimates the number of remaining tags and optimizes frame length accordingly to improve efficiency in large-scale RFID *** outcomes demonstrate that this proposed algorithmcan effectively break through the system throughput bottleneck of 36.8%,which is up to 30%higher than the existing DFSA standard scheme,and has more significant advantages,which is suitable for application in largescale RFID tags scenarios.

关键词： Radio frequency identification anti-collision tags grouping dynamic framed slotted Aloha

Passive IoT Localization Technology Based on SD-PDOA in NLOS and Multi-Path Environments

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Computers, Materials & Continua 2024年第7期80卷 913-930页

作者： Junyang Liu Yuan Li Yulu Zhang Shuai Ma Gui Li Yi He Haiwen Yi Yue Liu Xiaotao Xu Xu Zhang Jinyao He Guangjun Wen Jian Li School of Information and Communication Engineering/Yibin Institute University of Electronic Science and Technology of ChinaChengdu611731China Department of IoT Technology research China Mobile Research InstituteBeijing100053China The 10th research Institute of China Electronics Technology Group Corporation Chengdu611731China College of Information and Communication National University of Defense TechnologyWuhan430019China

Addressing the challenges of passive Radio Frequency Identification(RFID)indoor localization technology in Non-Line-of-Sight(NLoS)and multipath environments,this paper presents an innovative approach by introducing a combined technology integrating an improved Kalman Filter with Space Domain Phase Difference of Arrival(SD-PDOA)and Received Signal Strength Indicator(RSSI).This methodology utilizes the distinct channel characteristics in multipath and NLoS contexts to effectively filter out interference and accurately extract localization information,thereby facilitating high precision and stability in passive RFID *** efficacy of this approach is demonstrated through detailed simulations and empirical tests conducted on a custom-built experimental platform consisting of passive RFID tags and an R420 *** findings are significant:in NLoS conditions,the four-antenna localization system achieved a notable localization accuracy of 0.25 m at a distance of 5 *** complex multipath environments,this system achieved a localization accuracy of approximately 0.5 m at a distance of 5 *** compared to conventional passive localization methods,our proposed solution exhibits a substantial improvement in indoor localization accuracy under NLoS and multipath *** research provides a robust and effective technical solution for high-precision passive indoor localization in the Internet of Things(IoT)system,marking a significant advancement in the field.

关键词： Indoor localization multipath and NLoS environments combined SD-PDOA and RSSI technology

Landscape of global urban environmental resistome and its association with local socioeconomic and medical status

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Science China(Life Sciences) 2024年第6期67卷 1292-1301页

作者： Jun Wu Yige Hu Michael H Perlin David Danko Jun Lu Manuela Oliveira Johannes Werner Maria M Zambrano Maria A Sierra Olayinka O Osuolale Paweł Łabaj Nicolás Rascovan Nur Hazlin Hazrin-Chong Soojin Jang Haruo Suzuki Marina Nieto-Caballero Bharath Prithiviraj Patrick K H Lee Agnieszka Chmielarczyk Anna Różańska Yongxiang Zhao Lan Wang Christopher E Mason Tieliu Shi Center for Bioinformatics and Computational Biology and the Institute of Biomedical SciencesSchool of Life SciencesEast China Normal UniversityShanghai 200241China Department of Biology Program on Disease EvolutionUniversity of LouisvilleLouisville 40292USA Weill Cornell Medicine New York 10065USA The Bin Talal Bin Abdulaziz Alsaud Institute for Computational Biomedicine New York 10065USA Department of Pulmonary Medicine Shanghai Chest HospitalShanghai Jiao Tong UniversityShanghai 200025China i3S-Instituto de Investiga玢o e Inova玢o em Saúde Universidade do PortoPorto 4050-290Portugal Ipatimup-Instituto de Patologia e Imunologia Molecular da Universidade do Porto Porto 4200-465Portugal Departamento de Biologia Faculdade de CiênciasUniversidade do PortoPorto 4050-290Portugal High Performance and Cloud Computing Group Zentrum für Datenverarbeitung(ZDV)Eberhard Karls University of TübingenW鋍hterstra遝7672074TübingenGermany Corporación Corpogen research Center Bogotá110311Colombia Applied Environmental Metagenomics and Infectious Diseases research(AEMIDR) Department of Biological SciencesElizade UniversityIlara-Mokin 340271Nigeria Maopolska Centre of Biotechnology Jagiellonian UniversityKraków 30-005Poland Aix-Marseille Université Mediterranean Institute of OceanologyUniversitéde ToulonCNRSIRDUM 110Marseille 83041France Department of Biological Sciences and Biotechnology Faculty of Science and TechnologyUniversiti Kebangsaan Malaysia43600 UKM BangiSelangor Institut Pasteur Korea Seoul 13488Republic of Korea Faculty of Environment and Information Studies Keio UniversityFujisawaKanagawa 252-0882Japan University of Colorado at Boulder CivilEnvironmental and Architectural DepartmentBoulder 80303USA Brooklyn College of the City University of New York Brooklyn 11210USA School of Energy and Environment City University of Hong KongHong Kong 999077China Department of Microbiology Faculty of MedicineJagiellonian UniversityKraków 30-005Poland Biological Targeting Diagnosis and The

Antimicrobial resistance(AMR)poses a critical threat to global health and development,with environmental factors—particularly in urban areas—contributing significantly to the spread of antibiotic resistance genes(ARGs).However,most research to date has been conducted at a local level,leaving significant gaps in our understanding of the global status of antibiotic resistance in urban *** address this issue,we thoroughly analyzed a total of 86,213 ARGs detected within 4,728 metagenome samples,which were collected by the Meta SUB International Consortium involving diverse urban environments in 60 cities of 27 countries,utilizing a deep-learning based *** findings demonstrated the strong geographical specificity of urban environmental resistome,and their correlation with various local socioeconomic and medical *** also identified distinctive evolutionary patterns of ARG-related biosynthetic gene clusters(BGCs)across different countries,and discovered that the urban environment represents a rich source of novel *** study provides a comprehensive overview of the global urban environmental resistome,and fills a significant gap in our knowledge of large-scale urban antibiotic resistome analysis.

关键词： urban environment metagenome antibiotic resistance resistance diversity biosynthetic gene clusters

Endoscopic measurement of lesion size:An unmet clinical need

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Chinese Medical Journal 2024年第4期137卷 379-381页

作者： Yinong Zhu Wei Liu Lifan Zhang Bing Hu Department of Gastroenterology West China HospitalSichuan UniversityChengduSichuan 610041China

In recent years,the field of endoscopy has experienced significant technological *** developments have facilitated the efficient use of endoscopy for precise examination and minimally invasive treatment of various lesions.A key parameter considered during both endoscopic diagnosis and treatment is the size of the *** colon polypectomy,the size of the polyp is linked to the probability of malignancy and the likelihood of ***,in the case of early gastric cancer,larger tumors are often associated with an increased likelihood of lymph node metastasis,and whether to perform endoscopic submucosal dissection(ESD)is partially dependent on the size of the ***,accurate measurement of lesion size may significantly impact the diagnosis and treatment plan for the ***,at present,there is no standardized method for quantitative measurement during *** lack of a universal technique hinders the ability of clinical guidelines to provide precise recommendations for treatment.

关键词： diagnosis clinical size

research on the Strategic Path of Veterinary Medicine Serving Rural Revitalization in Agriculture-Related Universities

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Journal of Contemporary educational research 2024年第5期8卷 65-73页

作者： Jiedan Liao School of Life Science and Engineering Foshan UniversityFoshan 528225Guangdong ProvinceChina

Local agriculture-related universities must have certain regional advantages,advantages of agricultural characteristics,human resources,and science and technology in the process of implementing the rural revitalization *** literature research and investigation methods,combined with the experience of colleges and universities,the veterinary discipline of rural science and technology correspondent and technology yard,this study discusses our veterinary discipline in education teaching practice and talent training mode innovation,improving the quality of talent training and study on the strategic path of united front service for rural revitalization.

关键词： Veterinary medicine Rural revitalization Path research

Thyroid Hormone Biomonitoring:A Review on Their Metabolism and Machine-Learning Based Analysis on Effects of Endocrine Disrupting Chemicals

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Environment & Health 2024年第6期2卷 350-372页

作者： Shijie Chen Mengjie Yu Yiming Yao Yongcheng Li Ana He Zijun Zhou Liyang Pan Nan Xiao Haining Luo Hongwen Sun MOE Key Laboratory of Pollution Processes and Environmental Criteria College of Environmental Science and EngineeringNankai UniversityTianjin 300071China Tianjin Tianshi College Tianjin 301700China Dalian Center for Disease Control and Prevention DalianLiaoning Province 116000China Department of Center for Reproductive Medicine Tianjin Central Hospital Of Gynecology Obstetrics/Tianjin Key Laboratory Of Human Development And Reproductive RegulationTianjin 300052China

The thyroid is an essential endocrine organ in human body,and thyroid hormones(THs)are pivotal signaling molecules and mediators in various physiological ***,particularly in their free form,play a critical role in regulating body temperature and in the metabolism of lipid and glucose,making the maintenance of TH levels crucial for human *** undergo a series of metabolic processes,producing TH metabolites(THMs).THMs are significant in endocrine regulation,such as 3,5-diiothyronine(3,5-T2)and 3-iodothyronamine(3-T1AM),which exhibit activities akin to *** production and distribution of THMs are intricately linked to the function of specific organs and tissues,highlighting the need for advanced research into the determination and mechanisms of THMs in *** to endocrine disrupting chemicals(EDCs)can significantly affect the levels of thyroid stimulating hormone(TSH)and *** review utilizes machine learning to analyze epidemiological data,identifying potential EDCs that pose risks of hyperthyroidism and ***,it delves into the toxicological mechanisms of these EDCs,examining their effects on TH production,binding processes,related proteins,and metabolic *** approach effectively bridges the gap between epidemiological studies and toxicological researches,laying the groundwork for future research *** integrating epidemiological studies with machine learning,this review offers insightful perspectives on the potential risks associated with chemical exposure and underscores the necessity for further research in understanding the impact of EDCs on TH metabolism and TH-related health effects.

关键词： Thyroid stimulating hormone Emerging contaminants Data mining Thyroxine 3,3′,5-Triiodothyronine Homeostasis