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Distribution and localization of microfilament cytoskeleton is regulated by EBP50

The Chinese-German Journal of Clinical Oncology 2009年第5期8卷 282-285页

作者： Kun Liu Junfang Zheng Ying Wang Peng Chen Junqi He Department of Biochemistry and Molecular Biology Capital Medical University Beijing 100069 China Shandong Eye Institute Qjngdao 266071 China

Objective:To explore the influence of EBP50(ezrin-radixin-moesin-binding phospho-protein-50) on microfilament cytoskeleton content and distribution in cultured Hela cells, and to investigate the relationship between the changes in microfilament cytoskeleton localization and EBP50 after PDGF(platelet-derived growth factor) stimulation, and to further clarify the molecular mechanism by which EBP50 suppresses tumor cell proliferation and ***:pBK-CMV-HAEBP50 wild type recombinant plasmid and pBK-CMV-HA empty vector were transfected into Hela cells.G418 at 350 mg/L was used to screen for cell clones stably expressing *** blot was carried out to detect EBP50 *** and differences in microfilament cytoskeleton content and distribution in Hela cells transfected with pBK-CMV-HA-EBP50 wild type recombinant plasmid and pBK-CMV-HA empty vector were also treated with PDGF(10 ng/mL and 20 ng/mL, 37 ℃, 15 min) and stained by rhodamine-labeled phalloidin to observe the distribution of microfilament cytoskeleton in the two ***50 protein distribution in PDGF-stimulated Hela cells was detected by ***:Western blot results confirmed that the EBP50 cDNA fragment could express EBP50 in cultured Hela cell lines and that cell lines stably expressing EBP50 were successfully *** blot and fluorescence results showed that in the cell line transfected with empty vector, the microfilament cytoskeleton was thick, loose, multidirectional and displayed crossing *** content of microfilament cytoskeleton in the cell line transfected with pBK-CMV-HA-EBP50 was different from that found in the cell line transfected with empty ***50 expression enhanced microfilament cytoskeleton polymerization into compact thin *** the stimulation of PDGF, EBP50 migrated to the cell membrane from the cytosol together with microfilament cytoskeleton and co-localized ***:EBP50 can change the dist

关键词： microfilament cytoskeleton hela cell EBP50

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快捷两步法制备金纳米电极用于活体多巴胺检测（英文）

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电化学 2019年第2期25卷 244-251页

作者：关利浩王超张望蔡雨露李凯林雨青首都师范大学化学系北京100048

用微电极进行活体检测神经化学物质属于侵入式分析,会对脑组织产生不可避免的损伤,进而在生理上产生一些信号干扰检测过程.减小电极的尺寸对于减小对脑组织的损伤非常重要.该研究报道了一种新型制备金纳米电极的方法并将其用于活体鼠脑... 详细信息

用微电极进行活体检测神经化学物质属于侵入式分析,会对脑组织产生不可避免的损伤,进而在生理上产生一些信号干扰检测过程.减小电极的尺寸对于减小对脑组织的损伤非常重要.该研究报道了一种新型制备金纳米电极的方法并将其用于活体鼠脑内多巴胺分析研究.这种金纳米电极的制备过程包含两步:1)通过离子溅射在毛细管的尖端覆盖一层金种子;2)把覆盖有金种子的毛细管浸入氯金酸和盐酸羟胺混合溶液中湿法沉积生成连续导电金膜.制备好的纳米电极尖端约300～400 nm.该金纳米电极可以应用于多巴胺的检测,并且在多巴胺浓度1.0～56.0μmol·L^(-1)范围内有很好的线性响应,最低检测限低至0.14μmol·L^(-1)(信噪比=3).该金纳米电极具有优异的电化学性能,可以成功的应用于检测鼠脑纹状体儿茶酚胺的释放.

关键词：离子溅射化学沉积金纳米电极活体分析多巴胺

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Visualizing and Tracking Chinese Business Management and Economic Society High-Quality Development

Visualizing and Tracking Chinese Business Management and Eco...

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作者： Meijun Ning Yongxiang Liu Li Zhu Luoru Ma School of Economics and Management North China University of Technology National School of Development Peking University

The high-quality development of business management and economic society is the strategic target to the construction and dissemination of China’s national image. This research regarded CSSCI literatures of business management discipline in the CNKI database from 1978 to 2018 as the research objects. Using CiteSpace V to draw the maps of keyword co-occurrence, keyword cluster, burst detection, keyword timeline, this research investigated knowledge base, research hotspots, research frontiers and knowledge evolution of business management and economic society high-quality development. The research results are as follows.（1） The research framework for high-quality development is becoming completer and more practical, forming a research system with a combination of micro-business, medium-industry and macro-economy.（2） The most relevant and influential journals are Accounting Research and Business Management Journal.（3） Research hotspots mainly include business management, accounting information, cost, quality.（4） Research topics are grouped into 6 core paths: accounting information quality-business management, quality cost-business management, high-quality development-cost management, total quality management-customer satisfaction, high-quality development-total factor productivity, high-quality development-famous brand strategy.（5） Research frontiers mainly include business management, earning quality, corporate governance, internal control quality, total factor productivity.（6） Policy orientation guides business management and economic society high-quality development. This paper may help new researchers to identify the most relevant and influential journals and keywords, thus establishing future research directions. Besides, the paper may provide reference for further promoting business management and economic society high-quality development with Chinese features.

关键词： business management high-quality development economic society visualization analysis

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THE DATA PROCESSING OF OPTICAL SUPERVISORY CHANNEL IN DWDM SYSTEM

THE DATA PROCESSING OF OPTICAL SUPERVISORY CHANNEL IN DWDM S...

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中国光学学会2010年光学大会

作者： Xun Li,Yangan Zhang,Liming Zhou,Xueguang Yuan,Lili Hou,Minglun Zhang,Jinnan Zhang Key Laboratory of Information Photonics and Optical Communications,Ministry of Education,beijing University of Posts and Telecommunications,beijing

OSC （Optical Supervisory Channel） is a carrier of DWDM working statement information,including the information of network management,service and *** this paper,with the platform of ISE software and CPLD hardware,OSC data was successfully CMI encoded/decoded and scrambled/*** design has been applied in DWDM system reliably,which worked stably and showed a good performance.

关键词： DWDM OSC CPLD VHDL

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