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Chinese Chemical Letters 2022年第1期33卷 324-327页

作者： Yang Wang Qing Li Mengsheng Deng Kuijun Chen Jianmin Wang state key laboratory of trauma Burns and Combined InjuryDepartment of Surgical ResearchDaping HospitalArmy Medical UniversityChongqing 400042China School of Chemistry and Chemical Engineering Yangzhou UniversityYangzhou 225002China

Metal-organic frameworks(MOFs)have recently allured a variety of concern in the fields of ***,exploring their biomedical applications is still a relatively new *** this work,zeolite imidazole skeleton-8(ZIF-8)was reported for the first time as a drug carrier for the treatment of lung *** ZIF-8 nanoparticles encapsulating plumbagin(PLB)are achieved by a facile physical adsorption *** electron microscopy(SEM),powder X-ray diffraction(PXRD)and UV–vis absorption spectrum were conducted to investigate the physical properties of ZIF-8 and PLB@*** animal model,the collagen fibers deposition produced by severe lung injury is significantly *** secretion of inflammatory factor TGF-βand IL-6 were efficiently dropped by the combination of plumbagin and *** the same time,the expressions of collagen I,α-SMA and TNF-αwere also *** strategy puts forth a promising blueprint in the application of MOF materials,especially in biomedical fields.

关键词： ZIF-8 Plumbagin Drug delivery Lung injury

Impact and risk factors of sepsis on long-term outcomes after spontaneous intracerebral hemorrhage

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Chinese Medical Journal 2022年第8期135卷 1006-1008页

作者： Jie Lin Minglian He Binbin Tan Xue Yang Yuhong Li Jishu Xian Hua Feng Yujie Chen Department of Neurosurgery and state key laboratory of trauma Burn and Combined InjurySouthwest HospitalThird Military Medical University(Army Medical University)Chongqing 400038China Chongqing Clinical Research Center for Neurosurgery Southwest HospitalThird Military Medical University(Army Medical University)Chongqing 400038China Chongqing key laboratory of Precision Neuromedicine and Neuroregenaration Southwest HospitalThird Military Medical University(Army Medical University)Chongqing 400038China.

To the Editor:Spontaneous intracerebral hemorrhage(sICH)has a high incidence of acute cerebrovascular illness in elderly people in China,with high mortality and long-term functional impairment among ***,there is still controversy over whether secondary infection during the course of hospitalization after ICH has a negative impact on long-term function.

关键词： hemorrhage mortality intracerebral

Cyclophilin D-induced mitochondrial impairment confers axonal injury after intracerebral hemorrhage in mice

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Neural Regeneration Research 2023年第4期18卷 849-855页

作者： Yang Yang Kai-Yuan Zhang Xue-Zhu Chen Chuan-Yan Yang Ju Wang Xue-Jiao Lei Yu-Lian Quan Wei-Xiang Chen Heng-Li Zhao Li-Kun Yang Yu-Hai Wang Yu-Jie Chen Hua Feng Department of Neurosurgery The 904th Hospital of PLAAnhui Medical UniversityWuxiJiangsu ProvinceChina Department of Neurosurgery and state key laboratory of trauma Burn and Combined InjurySouthwest HospitalThird Military Medical University(Army Medical University)ChongqingChina Chongqing key laboratory of Precision Neuromedicine and Neuroregenaration Third Military Medical University(Army Medical University)ChongqingChina Wuxi Translational Medicine Center WuxiJiangsu ProvinceChina Department of Neurosurgery General Hospital of Xinjiang Military Command of PLAUrumqiXinjiang Uygur Autonomous RegionChina Department of Neurology The Second Medical Center&National Clinical Research Center for Geriatric DiseasesChinese PLA General HospitalBeijingChina

The mitochondrial permeability transition pore is a nonspecific transmembrane *** of mitochondrial permeability transition pore opening has been shown to alleviate mitochondrial swelling,calcium overload,and axonal *** D is an important component of the mitochondrial permeability transition *** cyclophilin D participates in mitochondrial impairment and axonal injury after intracerebral hemorrhage is not *** this study,we established mouse models of intracerebral hemorrhage in vivo by injection of autologous blood and oxyhemoglobin into the striatum in Thy1-YFP mice,in which pyramidal neurons and axons express yellow fluorescent *** also simulated intracerebral hemorrhage in vitro in PC12 cells using *** found that axonal degeneration in the early stage of intracerebral hemorrhage depended on mitochondrial swelling induced by cyclophilin D activation and mitochondrial permeability transition pore *** further investigated the mechanism underlying the role of cyclophilin D in mouse models and PC12 cell models of intracerebral *** found that both cyclosporin A inhibition and short hairpin RNA interference of cyclophilin D reduced mitochondrial permeability transition pore opening and mitochondrial *** addition,inhibition of cyclophilin D and mitochondrial permeability transition pore opening protected corticospinal tract integrity and alleviated motor dysfunction caused by intracerebral *** findings suggest that cyclophilin D is used as a key mediator of axonal degeneration after intracerebral hemorrhage;inhibition of cyclophilin D expression can protect mitochondrial structure and function and further alleviate corticospinal tract injury and motor dysfunction after intracerebral *** findings provide a therapeutic target for preventing axonal degeneration of white matter injury and subsequent functional impairment in central nervous diseases.

关键词： axonal injury corticospinal tract cyclophilin D cyclosporin A intracerebral hemorrhage mitochondrial impairment mitochondrial permeability transition pore motor dysfunction retraction bulb white matter

Emerging role of tumor cell plasticity in modifying therapeutic response

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Signal Transduction and Targeted Therapy 2020年第1期5卷 549-584页

作者： Siyuan Qin Jingwen Jiang Yi Lu Edouard CNice Canhua Huang Jian Zhang Weifeng He state key laboratory of Biotherapy and Cancer Center West China Hospitaland West China School of Basic Medical Sciences and Forensic MedicineSichuan Universityand Collaborative Innovation Center for Biotherapy610041 ChengduPeople’s Republic of China School of Medicine Southern University of Science and Technology ShenzhenShenzhenGuangdong 518055People’s Republic of China Guangdong Provincial key laboratory of Cell Microenvironment and Disease Research ShenzhenGuangdongPeople’s Republic of China Department of Biochemistry and Molecular Biology Monash UniversityClaytonVICAustralia School of Basic Medical Sciences Chengdu University of Traditional Chinese Medicine1166 Liutai Road611137 ChengduPeople’s Republic of China state key laboratory of trauma Burn and Combined InjuryInstitute of Burn ResearchSouthwest HospitalThird Military Medical University(Army Medical University)ChongqingPeople’s Republic of China Chongqing key laboratory for Disease Proteomics ChongqingPeople’s Republic of China

Resistance to cancer therapy is a major barrier to cancer *** views have proposed that acquisition of resistance may result from genetic ***,accumulating evidence implicates a key role of non-mutational resistance mechanisms underlying drug tolerance,the latter of which is the focus that will be discussed *** non-mutational processes are largely driven by tumor cell plasticity,which renders tumor cells insusceptible to the drug-targeted pathway,thereby facilitating the tumor cell survival and *** concept of tumor cell plasticity highlights the significance of re-activation of developmental programs that are closely correlated with epithelial–mesenchymal transition,acquisition properties of cancer stem cells,and transdifferentiation potential during drug *** observations in various cancers,this concept provides an opportunity for investigating the nature of anticancer drug *** the years,our understanding of the emerging role of phenotype switching in modifying therapeutic response has considerably *** expanded knowledge of tumor cell plasticity contributes to developing novel therapeutic strategies or combination therapy regimens using available anticancer drugs,which are likely to improve patient outcomes in clinical practice.

关键词： therapeutic plasticity thereby

Sub-cytotoxic concentrations of ionic silver promote the proliferation of human keratinocytes by inducing the production of reactive oxygen species

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Frontiers of Medicine 2018年第3期12卷 289-300页

作者： Xiaodong Duan Daizhi Peng Yilan Zhang Yalan Huang Xiao Liu Ruifu Li Xin Zhou Jing Liu Institute of burn Research Southwest Hospital Third Military Medical University Chongqing 400038 China burn and Plastic Surgery Department 209 Hospital of PLA Mudanjiang 157011 China Tissue Engineering Research Unit State Key Laboratory of Trauma Burns and Combined Injury Third Military Medical University Chongqing 400038 China

Silver-containing preparations are widely used in the management of skin wounds, but the effects of silver ions on skin wound healing remain poorly understood. This study investigated the effects of silver ions （Ag^＋） on the proliferation of human skin keratinocytes （HaCaT） and the production of intracellular reactive oxygen species （ROS）. After treating HaCaT cells with Ag^＋ and/or the active oxygen scavenger N-acetyl cysteine （NAC）, cell proliferation and intracellular ROS generation were assessed using CCK-8 reagent and DCFH-DA fluorescent probe, respectively. In addition, 5-bromo-2-deoxyUridine （BrdU） incorporation assays, cell cycle flow cytometry, and proliferating cell nuclear antigen （PCNA） immunocytochemistry were conducted to further evaluate the effects of sub-cytotoxic Ag^＋ concentrations on HaCaT cells. The proliferation of HaCaT cells was promoted in the presence of 10^-6 and 10^-5s mol/L Ag^＋at 24, 48, and 72 h. Intracellular ROS generation also significantly increased for 5 60 min after exposure to Ag^＋ The number of BrdU-positive cells and the presence of PCNA in HaCaT cells increased 48 h after the addition of 10^-6 and 10^-5 mol/L Ag^＋, with 10^-5 mol/L Ag^＋ markedly increasing the cell proliferation index. These effects of sub-cytotoxic Ag^＋ concentrations were repressed by 5 mmol/L NAC. Our results suggest that sub-cytotoxic Ag^＋ concentrations promote the proliferation of human keratinocytes and might be associated with a moderate increase in intracellular ROS levels. This study provides important experimental evidence for developing novel silver-based wound agents or dressings with few or no cytotoxicity.

关键词： ionic silver human keratinocyte cell proliferation reactive oxygen species active oxygen scavenger NAC

Electrotaxis of alveolar epithelial cells in direct-current electric fields

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Chinese Journal of traumatology 2023年第3期26卷 155-161页

作者： Chao-Yue Yang Jian-Hui Sun Kan Zhu Juan Du Ying Zhang Cong-Hua Lu Wen-Yi Liu Ke-jun Zhang An-Qiang Zhang Ling Zeng Jian-Xin Jiang Li Li Department of Respiratory Medicine Daping HospitalThird Military Medical University(Army Medical University).Chongqing400042China state key laboratory of trauma Burnsand Combined InjuryDepartment of Trauma Medical CenterDaping HospitalThird Military Medical University(Army Medical University)Chongqing400042China Department of Outpatients Daping HospitalThird Military Medical University(Army Medical University)Chongqing400042China

Purpose:This study aims to elucidate the electrotaxis response of alveolar epithelial cells(AECs)in direct-current electric fields(EFs),explore the impact of EFs on the cell fate of AECs,and lay the foundation for future exploitation of EFs for the treatment of acute lung ***:AECs were extracted from rat lung tissues using magnetic-activated cell *** elucidate the electrotaxis responses of AECs,different voltages of EFs(0,50,100,and 200 mV/mm)were applied to two types of AECs,*** migrations were recorded and trajectories were pooled to better demonstrate cellular activities through *** directionality was calculated as the cosine value of the angle formed by the EF vector and cell *** further demonstrate the impact of EFs on the pulmonary tissue,the human bronchial epithelial cells transformed with Ad12-SV402B(BEAS-2B cells)were obtained and experimented under the same conditions as *** determine the influence on cell fate,cells underwent electric stimulation were collected to perform Western blot ***:The successful separation and culturing of AECs were confirmed through immunofluorescence *** with the control,AECs in EFs demonstrated a significant directionality in a voltage-dependent *** general,type I alveolar epithelial cells migrated faster than type II alveolar epithelial cells,and under EFs,these two types of cells exhibited different response *** type II alveolar epithelial cells,only EFs at 200 mV/mm resulted a significant difference to the velocity,whereas for,EFs at both 100 mV/mm and 200 mV/mm gave rise to a significant *** blotting suggested that EFs led to an increased expression of a AKT and myeloid leukemia 1 and a decreased expression of Bcl-2-associated X protein and Bcl-2-like protein ***:EFs could guide and accelerate the directional migration of AECs and exert antiapoptotic effects,which indicated that EFs are important biophysica

关键词： Alveolar epithelial cells Electric fields Migration Electrotaxis Acute lung injury

Recent advances in nanotherapeutics for the treatment of burn wounds

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burns & trauma 2021年第1期9卷 220-239页

作者： Rong Huang Jun Hu Wei Qian Liang Chen Dinglin Zhang Department of Chemistry College of Basic MedicineThird Military Medical University(Army Medical University)Chongqing 400038China Department of Neurology Southwest HospitalThird Military Medical University(Army Medical University)Chongqing 400038China Institute of burn Research Southwest HospitalThird Military Medical University(Army Medical University)Chongqing 400038China Department of plastic surgery Southwest HospitalThird Military Medical University(Army Medical University)Chongqing 400038China state key laboratory of trauma Burn and Combined InjuryChongqing400038China

Moderate or severe burns are potentially devastating injuries that can even cause death,and many of them occur every *** prevention,anti-inflammation,pain management and administration of growth factors play key roles in the treatment of burn *** therapeutic strategies under development,such as nanotherapeutics,are promising prospects for burn wound ***,including metallic and polymeric nanoformulations,have been extensively developed to manage various types of *** human and animal studies have demonstrated that nanotherapeutics are biocompatible and effective in this ***,we provide comprehensive knowledge of and an update on the progress of various nanoformulations for the treatment of burn wounds.

关键词： burn wounds Metal and metal oxide nanotherapeutics Polymeric nanotherapeutics Therapeutic mechanism Wound healing

Effects of early rehabilitation in improvement of paediatric burnt hands function

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World Journal of Clinical Cases 2021年第32期9卷 9741-9751页

作者： Ya-Qin Zhou Jun-Yi Zhou Gao-Xing Luo Jiang-Lin Tan Institute of burn Research State Key Laboratory of TraumaBurns and Combined InjuriesChongqing Key Laboratory for Disease ProteomicsSouthwest HospitalThe Third Military Medical University(Army Medical University)Chongqing 400038China

BACKGROUND Hands are one of the most common burn sites in *** scar contractures in hands after wound healing result in further reductions in their range of motion(ROM),motility,and fine motor *** can improve the function of *** the optimal time of rehabilitation intervention is still ***,this study was designed to investigate the effects of early rehabilitation management of paediatric burnt hands and to compare the efficacy between early and later rehabilitation *** To investigate the effects of early rehabilitation management of paediatric burnt *** A total of 52 children with burnt hands were allocated into the early intervention group(≤1 mo from onset)and a late intervention group(>1 mo from onset)between January 2016 and December *** children received the same rehabilitation programme including skin care,scar massage,passive ROM exercises,active ROM exercises,compression therapy,orthotic devices wearing and game or music *** assessments were performed before and after the rehabilitation *** In the early intervention group,the ROM of the hands was significantly improved after rehabilitation(P=0.001).But in the late group the effect was not significant statistically(P=0.142).In the early group,38.5%of the patients showed significant improvement,while in the late group,69.2%of the patients showed no significant *** time from onset to posttraumatic rehabilitation(P=0.0007)and length of hospital stay(P=0.003)were negatively correlated with the hand function *** length of rehabilitation stay was positively correlated with the hand function improvement(P=0.005).CONCLUSION These findings suggest that early rehabilitation might show better results in terms of ROM.

关键词： burns Children Rehabilitation Range of motion Hand flexion Hand extension

Morphological study of Schwann cells remyelination in contused spinal cord of rats

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Chinese Journal of traumatology 2013年第4期16卷 225-229页

作者： LI Yue ZHANG Lu ZHANG Jie-yuan LIU Zheng DUAN Zhao-xia LI Bing-cang Neurosurgery Department 451 Hospital of ChinesePeople's Liberation Army Xi'an 710054 China Life School Southwest University Chongqing 400715China state key laboratory of trauma Burn and CombinedInjury Research Institute of Surgery and Daping HospitalThird Military Medical University Chongqing 400042 China Physiological Department Chongqing MedicalUniversity Chongqing 400042 China

Abstract：Objective:To study the role and effect of Schwann cells (SCs) remyelination in contused spinal ***:Green fluorescence protein expressing-SCs were transplanted into the epicenter,rostral and caudal tissues of the injury site at 1 week after the spinal cords were *** 6 weeks,the spinal cords were removed for cryosections,semithin sections and ultrathin sections,and then immunocytochemical staining of myelin basic protein (MBP),P0 protein (P0) and S 100 protein (S100) was carried out on the *** and semiquantitative analyses were performed on the cryosections and semithin *** ofmyelinated fibers was observed on the ultrathin sections under electron ***:Transplanted SCs and myelinated fibers immunocytochemically labeled by MBP,P0 as well as S100 distributed in whole injured *** quantity of myelinated fibers labeled by the three myelin proteins showed no statistical difference,however,which was significantly larger than that of *** the semithin sections,the experimental group demonstrated more myelinated fibers in the injured area than the controls,but the fibers had smaller diameter and thinner myelin sheath under electron ***:SCs can promote regeneration of injured nerve fibers and enhance remyelination,which may be histological basis of SCs-mediated functional repair of injured spinal cords。

关键词：髓鞘碱性蛋白脊髓损伤雪旺氏细胞挫伤形态学电子显微镜大鼠蛋白表达

The mobilization of rat’s mesenchymal stem cells into peripheral blood by LiCL and its potency differentiation

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Chinese Science Bulletin 2008年第17期53卷 2632-2638页

作者： DENG dun ZOU ZhongMin ZHOU TaoLi AI GuoPing WANG JunPing DONG ShiWu SU YongPing Institute of combined injury State Key Laboratory of Trauma Burns and Combined Injury Department of Preventive Medicine Third Military Medical University Chongqing 400038 China Department of Pathogenic Biology Third Military Medical University Chongqing 400038 China

Mesenchymal stem cells (MSCs) are multipotent cells, which can differentiate into different tissues. It is still controversial whether MSCs can be isolated from adult peripheral blood (PB) under normal conditions and whether they can be mobilized in a way similar to that of hematopoietic stem cells (HSCs). In this study, rat MSCs circulating in the PB under normal conditions can be isolated and cultured, and MSCs from PB and BM can be mobilized by LiCL. The mobilized MSCs can be induced to differentiate into neuron by such factors as β-mercaptoethanol and supernatants of nerve cell cultures. The present study provides a broader perspective on the repair of neural trauma.

关键词：间叶细胞干细胞外周血 LiCL 动物实验