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Deep learning identification of novel autophagic protein-protein interactions and experimental validation of Beclin 2-Ubiquilin 1 axis in triple-negative breast cancer

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Oncology research 2025年第1期33卷 67-81页

作者： Xiang Li Wenke Jin Lifeng Wu Huan Wang Xin Xie Wei Huang B O Liu State Key Laboratory of Southwestern Chinese Medicine Resources School of Pharmacy and College of Medical Technology Chengdu University of Traditional Chinese Medicine Chengdu 611137 China. Department of Biotherapy Cancer Center and State Key Laboratory of Biotherapy West China Hospital Sichuan University Chengdu 610041 China.

Background:Triple-negative breast cancer (TNBC), characterized by its lack of traditional hormone receptors and HER2, presents a significant challenge in oncology due to its poor response to conventional therapies. Autophagy is an important process for maintaining cellular homeostasis, and there are currently autophagy biomarkers that play an effective role in the clinical treatment of tumors. In contrast to targeting protein activity, intervention with protein-protein interaction (PPI) can avoid unrelated crosstalk and regulate the autophagy process with minimal interference pathways.Methods:Here, we employed Naive Bayes, Decision Tree, and k-Nearest Neighbors to elucidate the complex PPI network associated with autophagy in TNBC, aiming to uncover novel therapeutic targets. Meanwhile, the candidate proteins interacting with Beclin 2 were initially screened in MDA-MB-231 cells using Beclin 2 as bait protein by immunoprecipitation-mass spectrometry assay, and the interaction relationship was verified by molecular docking and CO-IP experiments after intersection. Colony formation, cellular immunofluorescence, cell scratch and 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) tests were used to predict the clinical therapeutic effects of manipulating candidate PPI.Results:By developing three PPI classification models and analyzing over 13,000 datasets, we identified 3733 previously unknown autophagy-related PPIs. Our network analysis revealed the central role of Beclin 2 in autophagy regulation, uncovering its interactions with 39 newly identified proteins. Notably, the CO-IP studies identified the substantial interaction between Beclin 2 and Ubiquilin 1, which was anticipated by our model and discovered in immunoprecipitation-mass spectrometry assay results. Subsequently, investigations showed that overexpressing Beclin 2 increased Ubiquilin 1, promoted autophagy-dependent cell death, and inhibited proliferation and metastasis in MDA-MB-231 cells.

关键词： Artificial intelligence (AI) Autophagy Beclin 2 Protein-protein interactions (PPI) Triple-negative breast cancer (TNBC) Ubiquilin 1

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Bacterial indole-3-propionic acid inhibits macrophage IL-1β production through targeting methionine metabolism

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science China Life sciences 2025年

作者： Ziyi Han Jian Fu Aiyan Gong Wenkai Ren State Key Laboratory of Livestock and Poultry Breeding College of Animal Science South China Agricultural University Animal Disease-resistant Nutrition Key Laboratory of Sichuan Province Sichuan Agricultural University China Institute of Veterinary Drug Control

The gut microbiota plays key roles in host health by shaping the host immune responses through their metabolites, like indole derivatives from tryptophan. However, the direct role of these indole derivatives in macrophage fate decision and the underlying mechanism remains unknown. Here, we found that bacterial indole-3-propionic acid(IPA) downregulates interleukin-1beta(IL-1β) production in M1 macrophages through inhibition of nuclear factor-kappa B(NF-κB) signaling. Mechanistically, IPA binds specifically with methionine adenosyltransferase 2A(MAT2A) to promote S-adenosylmethionine(SAM) synthesis, which facilitates the DNA methylation of ubiquitin-specific peptidase 16(USP16, a deubiquitinase), and in turn promotes Toll-like receptor 4(TLR4) ubiquitination and NF-κB inhibition. Furthermore,IPA administration attenuates sepsis in mouse models induced by lipopolysaccharides(LPS), showcasing its potential as a microbial-derived adjunct in alleviating inflammation. Collectively, our findings reveal a newly found microbial metabolite-immune system regulatory pathway mediated by IPA.

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适用于狭小空间的非接触式传感仿生软体机器人（英文）

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science China Materials 2025年

作者：肖宇航周子淞潘兴海刘艳玲梅浩王浩伦蒲伟 Key Laboratory of Advanced Spatial Mechanism and Intelligent Spacecraft Ministry of EducationSchool of Aeronautics and AstronauticsSichuan University Robotic Satellite Laboratory School of Aeronautics and AstronauticsSichuan University

近年来,软体机器人概念的提出使得研究者们开始借鉴动物的运动和感知能力,开发出结合生物视觉、触觉等特性的仿生软体机器人,以实现操控、避障和路径规划.软体机器人小型化是近些年来的重点研究领域,然而,受限于较小的体积使其只能具备...

近年来,软体机器人概念的提出使得研究者们开始借鉴动物的运动和感知能力,开发出结合生物视觉、触觉等特性的仿生软体机器人,以实现操控、避障和路径规划.软体机器人小型化是近些年来的重点研究领域,然而,受限于较小的体积使其只能具备单一的行走运动能力,未真正实现仿生机器人的功能集成化.本研究受到自然界中昆虫运动和电感知能力的启发,开发了一款小型且轻质量的(900 mg)电热驱动(ETA)具备电容式非接触传感的软体机器人.电热驱动的PE/CNT/PI复合薄膜驱动器,具备优良的机械性能和灵活性,能实现最大弯曲角度达320°,展现出至少支撑自身重量5倍的负载能力.传感器部分由直径0.2 mm的银丝构成,通过边缘电场的变化检测物体的靠近,信号变化响应灵敏,能有效识别不同直径的障碍物.与其他报道的工作相比,该软体机器人聚焦于检测外部环境,在狭小空间和光线昏暗的环境中具备障碍物感知能力,它还利用在运动过程中生成传感器信号变化来获取有关自身运动姿势的信息,为未来在复杂环境中的应用提供了新的思路与依据.

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Exploring the interaction between the gut microbiota and cyclic adenosine monophosphate-protein kinase A signaling pathway:a potential therapeutic approach for neurodegenerative diseases

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Neural Regeneration Research 2025年第11期20卷 3095-3112页

作者： Fengcheng Deng Dan Yang Lingxi Qing Yifei Chen Jilian Zou Meiling Jia Qian Wang Runda Jiang Lihua Huang College of Pharmacy Chengdu University of Traditional Chinese MedicineChengduSichuan ProvinceChina State Key Laboratory of Southwestern Chinese Medicine Resources Chengdu University of Traditional Chinese MedicineChengduSichuan ProvinceChina

The interaction between the gut microbiota and cyclic adenosine monophosphate(cAMP)-protein kinase A(PKA)signaling pathway in the host's central nervous system plays a crucial role in neurological diseases and enhances communication along the gut–brain *** gut microbiota influences the cAMP-PKA signaling pathway through its metabolites,which activates the vagus nerve and modulates the immune and neuroendocrine ***,alterations in the cAMP-PKA signaling pathway can affect the composition of the gut microbiota,creating a dynamic network of microbial-host *** reciprocal regulation affects neurodevelopment,neurotransmitter control,and behavioral traits,thus playing a role in the modulation of neurological *** coordinated activity of the gut microbiota and the cAMP-PKA signaling pathway regulates processes such as amyloid-β protein aggregation,mitochondrial dysfunction,abnormal energy metabolism,microglial activation,oxidative stress,and neurotransmitter release,which collectively influence the onset and progression of neurological *** study explores the complex interplay between the gut microbiota and cAMP-PKA signaling pathway,along with its implications for potential therapeutic interventions in neurological *** pharmacological research has shown that restoring the balance between gut flora and cAMP-PKA signaling pathway may improve outcomes in neurodegenerative diseases and emotional *** can be achieved through various methods such as dietary modifications,probiotic supplements,Chinese herbal extracts,combinations of Chinese herbs,and innovative dosage *** findings suggest that regulating the gut microbiota and cAMP-PKA signaling pathway may provide valuable evidence for developing novel therapeutic approaches for neurodegenerative diseases.

关键词： cyclic adenosine monophosphate emotional disorders gut microbiota neurodegenerative diseases neurological diseases protein kinase A reciprocal regulation signaling pathway strategy therapies

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Melting Evaluation of Phase Change Materials Impregnated into Cascaded Metal Foams with Regionalized Enhancement

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Journal of Thermal science 2025年第1期 223-241页

作者： WEI Xinyi ZHANG Nan ZHANG Zhaoli CAO Xiaoling YUAN Yanping School of Mechanical Engineering Southwest Jiaotong University

Metal foam promotes the heat transfer of phase change materials(PCMs) in the penalty of reducing the energy storage density of the composite *** this work,the effects of constant porosity(0.96,0.94,0.92,or 0.90) and pore density(PPI) of metal foam on heat transfer of composite PCMs are *** rate could be enhanced by employing with low porosity copper ***,aiming to the right bottom phase changing "dead region",a regionalized enhancement strategy of cascaded metal foams is *** dynamic melting performances of all the composite PCMs are comprehensively *** results reveal that the cascaded configuration is beneficial for *** show that the horizontal strategy enhances melting performance:a maximum of 17.98% reduction in total melting time could be reached when the rear part porosity is 90%.The energy storage density rate could be raised by 5.48%.Besides,the vertical strategy performs with a better average temperature uniformity of 0.441 and brings a lower temperature in the heated *** sum up,the regionalized enhancement of copper foam provides better performance in the phase change *** shows significant potential for solar heat storage and thermal protection.

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Elevated temperature properties of Mg-12Li-Al-MgO composites

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中国有色金属学会会刊：英文版 2006年第2期16卷 432-437页

作者：魏晓伟黄清民 Department of Materials science and Engineering Xihua University Chengdu 610039 China

The compressive creep of Mg-12Li-Al-MgO particulate composites was investigated, which were produced by the reaction of reinforcement materials (B2O3) with Mg-12Li-Al alloy melt in the temperature range of 100?190 ℃ and under different compressive stress in the range of 40?70 MPa with special apparatus. The content of MgO particulates is about 0, 5%, 10%, 15%(volume fraction) in Mg-12Li-Al alloy respectively. The results reveal that the creep resistance of the particulate composites is increased with increasing the content of MgO particulates and considerable improvement in creep resistance is observed in Mg-12Li-Al-MgO composites. However, over all range of temperatures and stresses, the creep data for these composites can be correlated using an empirical equation ε s=Aσnexp(?Q/RT), where n is 4.93 and Q is about 78.1 kJ/mol for Mg-14Li-Al alloy and n is between 7.48 and 9.47 and Q is 111.2?137.3 kJ/mol for Mg-12Li-Al-MgO composites. The different compressive creep behavior of the composites is associated with the different material constant A. The compressive creep rate is controlled by the lattice diffusion of Li and dislocation climb.

关键词： Mg-12Li-Al 镁合金氧化镁微粒压缩蠕变显微结构

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Adjoint based state estimation of compressible flow in porous media

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Petroleum 2021年第1期7卷 39-52页

作者： Yanbin Sun Zhibin Liu Yanjie Hu School of science Southwest Petroleum UniversityChengdu610500China

In this paper,we study the state estimation of compressible single phase flow in compressible porous *** initial pressure distribution is estimated according to discrete adjoint approach based on the collected well pressure *** first-order Tykhonov regularization method is used to obtain reasonable *** analyzing the optimality condition of estimation problem,the discrete adjoint state equation and discrete adjoint gradient are derived based on the numerical scheme of the continuous equations.A quasi-Newton numerical optimization method related to adjoint gradient is proposed to solve the estimation *** estimation results with different regularization coefficients are compared and analyzed by numerical *** deviation between the estimated pressure obtained without regularization and the real pressure is *** result with smaller deviation and higher smoothness can be obtained through appropriate regularization *** the observation error is large,the observed values generated by the estimated pressure fit well with the real pressure.

关键词： Regularization Discrete adjoint approach State estimation Single phase compressible flow Porous media

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Extrusion-base d 3D-printe d“rolle d-up”composite scaffolds with hierarchical pore structure for bone growth and repair

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Journal of Materials science & technology 2024年第4期171卷 222-234页

作者： Yufan Li Li Chen Yijing Stehle Mingyue Lin Chenxin Wang Rui Zhang Min Huang Yubao Li Qin Zou Research Center for Nano Biomaterials Analytical&Testing CenterSichuan UniversityChengdu 610064China Analytical&Testing Center Sichuan UniversityChengdu 610064China Department of mechanical engineering Union College807 Union St.SchenectadyNY 12308USA sichuan Institute of Atomic Energy Chengdu 610101China

Three-dimensional(3D)bioprinting,specifically direct ink writing(DIW)capable of printing biologically active substances such as growth factors or drugs under low-temperature conditions,is an emerging di-rection in bone tissue ***,limited by the bio-ink mobility and the poor resolution of this printing technology,the lateral pores of current crisscross-stacked scaffolds printed through DIW tend to clog and are inimical to bone ***,it is critical to develop DIW printed biologi-cal scaffold structure with high mechanical strength,porosity,and biocompatibility ***,patterned polylactic acid(PLA)/polycaprolactone(PCL)/nano-hydroxyapatite(n-HA)based scaffold was printed through DIW technological and rolled-up for properties characterization,cytocompatibility test,and bone repair *** result not only shows that the hexagonal patterned scaffolds are me-chanically strong with porosity,but also demonstrated that the hierarchical pore structure formed during rolled-up has the potential to address the clogging problem and stimulates bone growth and repair.

关键词： Direct ink writing(DIW) Bone repair scaffold,Micro-CT Bio composites

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Design and characteristics of a new type laminar plasma torch for materials processing

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Plasma science and technology 2020年第1期22卷 24-32页

作者： Xiuquan CAO Lin CHEN School of Mechanical Engineering Sichuan University of Science and EngineeringZigong 643000People's Republic of China

Laminar plasma jet(LPJ)generated by laminar plasma torch(LPT)has a favorable temperature and velocity ***,it is superior to the turbulent plasma jet in material ***,most of the reported LPTs usually operate at a relatively low output power with a relatively low arc voltage and thermal efficiency,which limits its *** this context,this paper attempts to design a new type of high-power LPT with a relatively low arc current and a high thermal *** the first section,the design principle of the main components is studied and discussed in detail,and a new high-power LPT is ***,the experimental characteristics of the proposed high-power LPT are *** results reveal the following characteristics of the proposed LPT.(1)The max jet length of the proposed LPT reaches at 540 mm.(2)Its mean arc voltage is higher than 290 V when the LPT works with arc currents lower than 200 *** to an output power greater than 50 kW.(3)The mean thermal efficiency is higher than ***,the proposed LPT has been applied to spheroidize the aluminum oxide *** experiment results for the production of spherical powders show that the proposed LPT hits a good characteristic for material processing.

关键词： laminar plasma torch design principles high arc voltage with a low arc current spheroidisation

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High catalytic performance of neodymium modified Co_(3)O_(4) for toluene oxidation

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Journal of Rare Earths 2024年第1期42卷 94-101,I0004页

作者： Yanqiong Hu Luyue Zhang Jianbei Zhang Yongjun Liu School of Architecture and Environment Sichuan UniversityChengdu610065China National Engineering Research Center for Flue Gas Desulfurization Chengdu610065China

Co-based catalysts are the most promising catalysts in catalytic oxidation of volatile organic compounds(VOCs).Precious metal doping is adopted to improve the catalytic activity of toluene on Co_(3)O_(4) catalysts,but greatly increases its cost along with *** is found that doping a small amount of rare earth(Ce,Pr,Sm and Nd)can dramatically promote the catalytic activity of Co_(3)O_(4).Especially,the Nd-doped Co_(3)O_(4) catalyst exhibits excellent catalytic activity with a toluene removal rate of 90% at 162.1℃,which is even better than that of Pt-doped Co_(3)O_(4).Compared with other rare earth metal doping,the Nd doping leads to a higher ratio of Co^(3+)/Co^(2+) and has more oxygen *** in situ diffuse reflectance infrared Fourier transform spectroscopy(DRIFTS)experiments show that the lattice oxygen of Nd-Co sample can be utilized at a quite low temperature,while that of pure Co_(3)O_(4) cannot engage in oxidation reaction when the temperature is below 200℃,which visually demonstrates the main reason for the improved catalytic performance of Nd-Co catalyst.

关键词： Rare earth metals Nd Co_(3)O_(4) Toluene catalytic oxidation Oxygen vacancy Lattice oxygen

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