检索结果-南通市图书馆

Phosphatidic Acid （PA） PP2A Activity and PIN1 Binds PP2AA1 to Regulate Polar Localization

在线全文

学校读者我要写书评

暂无评论

Molecular Plant 2013年第5期6卷 1692-1702页

作者： Hong-Bo Gao Yu-Jia Chu Hong-Wei Xue National Key Laboratory of Plant Molecular Genetics Institute of Plant Physiology and Ecology Shanghai Institutes for Biological Sciences Chineseacademy of Sciences 200032 Shanghai People's Republic of China

Phospholipase D （PLD） exerts broad biological functions in eukaryotes through regulating downstream effectors by its product, phosphatidic acid （PA）. Protein kinases and phosphatases, such as mammalian target of rapa- mycin （mTOR）, Protein Phosphatase 1 （PP1） and Protein Phosphatase 2C （PP2C）, are PA-binding proteins that execute crucial regulatory functions in both animals and plants. PA participates in many signaling pathways by modulating the enzymatic activity and/or subcellular localization of bound proteins. In this study, we demonstrated that PLD-derived PA interacts with the scaffolding A1 subunit of Protein Phosphatase 2A （PP2A） and regulates PP2A-mediated PIN1 dephos- phorylation in Arabidopsis. Genetic and pharmacological studies showed that both PA and PP2A participate in the regu- lation of auxin distribution. In addition, both the phosphorylation status and polar localization of PIN1 protein were affected by PLD inhibitors, Exogenous PA triggered the membrane accumulation of PP2AA1 and enhanced the PP2A activity at membrane, while PLD inhibition resulted in the reduced endosomal localization and perinuclear aggregation of PP2AA1. These results demonstrate the important role of PLD-derived PA in normal PP2A-mediated PIN dephosphoryl- ation and reveal a novel mechanism, in which PA recruits PP2AA1 to the membrane system and regulates PP2A function on membrane-targeted proteins. As PA and PP2A are conserved among eukaryotes, other organisms might use similar mechanisms to mediate multiple biological processes.

关键词： phosphatidic acid (PA) PP2A PIN1 phosphorylation polar localization.

Structure of human lysine methyltransferase Smyd2 reveals insights into the substrate divergence in Smyd proteins

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Molecular Cell Biology 2011年第5期3卷 293-300页

作者： Shutong Xu Chen Zhong Tianlong Zhang Jianping Ding State Key Laboratory of Molecular Biology and Research Center for Structural Biology Institute of Biochemistry and Cell BiologyShanghai Institutes for Biological SciencesChinese Academy of SciencesShanghai 200031China Graduate School of Chinese Academy of Sciences 320 YueYang RoadShanghai 200031China

The SET-and myeloid-Nervy-DEAF-1(MYND)-domain containing(Smyd)lysine methyltransferases 1–3 share relatively high sequence similarity but exhibit divergence in the substrate *** we report the crystal structure of the full-length human Smyd2 in complex with S-adenosyl-L-homocysteine(AdoHcy).Although the Smyd1–3 enzymes are similar in the overall structure,detailed comparisons demonstrate that they differ substantially in the potential substrate-binding *** binding site of Smyd3 consists mainly of a deep and narrow pocket,while those of Smyd1 and Smyd2 consist of a comparable pocket and a long *** addition,Smyd2,which has lysine methyltransferase activity on histone H3-lysine 36,exhibits substantial differences in the wall of the substrate-binding pocket compared with those of Smyd1 and Smyd3 which have activity specifically on histone H3-lysine *** differences in the substrate-binding site might account for the observed divergence in the specificity and methylation state of the *** modeling study of Smyd2 in complex with a p53 peptide indicates that mono-methylation of p53-Lys372 might result in steric conflict of the methyl group with the surrounding residues of Smyd2,providing a structural explanation for the inhibitory effect of the SET7/9-mediated mono-methylation of p53-Lys372 on the Smyd2-mediated methylation of p53-Lys370.

关键词： Smyd2 Smyd proteins SET family histone lysine methyltransferase epigenetics

Improved plasmid-based recovery of coxsackievirus A16 infectious clone driven by human RNA polymerase I promoter

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Virologica Sinica 2016年第4期31卷 339-341页

作者： xiaoli wang chaoyun shen tan chen ke lan zhong huang yunfang zhang qingwei liu vaccine research center key laboratory of molecular virology & immunologyinstitut pasteur of shanghaishanghai institutes for biological scienceschinese academy of sciencesShanghai 200031China

Dear Editor,Coxsackievirus A16(CA16)is one of the major viral pathogens associated with hand,foot,and mouth ***16 belongs to the Enterovirus genus of the Picornaviridae family and possesses a single-stranded positivesense RNA genome(Mao et al.,2014).Reverse genetics is an important tool for CA16 ***,a reverse genetics T7 polymerase-based system was de-

关键词： RNA Figure Improved plasmid-based recovery of coxsackievirus A16 infectious clone driven by human RNA polymerase I promoter MD CA CPE Vero

Thymine DNA glycosylase promotes transactivation of β-catenin/TCFs by cooperating with CBP

在线全文

学校读者我要写书评

暂无评论

Journal of Molecular Cell Biology 2014年第3期8卷 231-239页

作者： Yingying Jia Fen Nie Aiying Du Zhangcheng Chen Yuanbo Qin Tao Huang Xiaomin Song Lin Li State Key Laboratory of Molecular Biology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of SciencesShanghai 200031 China

Thymine DNA glycosylase CrDG）, an enzyme that initiates the repair of G/T and G/U mismatches, has been lately found crucial in em- bryonic development to maintain epigenetic stability and facilitate the active DNA demethylation. Here we report a novel role of TDG in Wnt signaling as a transcriptional coactivator of β-catenin/TCFs complex. Our data show that TDG binds to the transcriptional factor family LEF1/TCFs and potentiates β-catenin/TCFs transactivation, while TDG depletion suppresses Wnt3a-stimulated reporter activity or target gene transcription. Next, we show that CBP, a known coactivator, is also required for TDG function through forming a coopera- tive complex on target promoters. Moreover, there is an elevation of TDG levels in human colon cancer tissue, and knockdown of TDG inhibits proliferation of the colon cells. Overall, our results reveal that TDG, as a new coactivator, promotes β-catenin/TCFs transacti- vation and functionally cooperates with CBP in canonical Wnt signaUng.

关键词： TDG TCF CBP Wnt signaling transactivation

Multiple Components are Integrated to Determine Leaf Complexity in Lotus japonicus

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Integrative Plant Biology 2013年第5期55卷 419-433页

作者： Zhenhua Wang Jianghua Chen Lin Weng Xin Li Xianglin Cao Xiaohe Hu Da Luo Jun Yang National Key Laboratory of Plant Molecular Genetics Institute of Plant Physiology and EcologyShanghai Institutes for Biological SciencesGraduate School of the Chinese Academy of Sciencesthe Chinese Academy of Sciences State Key Laboratory of Biocontrol School of Life SciencesSun Yat-sen University

Transcription factors and phytohormones have been reported to play crucial roles to regulate leaf complexity among plant species. Using the compound-leafed species Lotus japonicus, a model legume plant with five visible leaflets, we characterized four independent mutants with reduced leaf complexity, proliferating floral meristem （pfm）, proliferating floral organ-2 （pfo-2）, fused leaflets1 （ful1） and umbrella leaflets （uml）, which were further identified as loss-of-function mutants of Arabidopsis orthologs LEAFY （LFY ）, UNUSUAL FLORAL ORGANS （UFO）, CUP-SHAPED COTYLEDON 2 （CUC2） and PIN-FORMED 1 （PIN1）, respectively. Comparing the leaf development of wild-type and mutants by a scanning electron microscopy approach, leaflet initiation and/or dissection were found to be affected in these mutants. Expression and phenotype analysis indicated that PFM/LjLFY and PFO/LjUFO determined the basipetal leaflet initiation manner in L. japonicus. Genetic analysis of ful1 and uml mutants and their double mutants revealed that the CUC2-like gene and auxin pathway also participated in leaflet dissection in L. japonicus, and their functions might influence cytokinin biogenesis directly or indirectly. Our results here suggest that multiple genes were interplayed and played conserved functions in controlling leaf complexity during compound leaf development in L. japonicus.

关键词： Compound leaf FUSED LEAFLETS1 Lotus japonicus PROLIFERATING FLORAL ORGAN/LjUFO PROLIFERATING FLORAL MERISTEM/LjLFY UMBRELLA LEAFLETS.

A Single Amino-Acid Substitution at Lysine 40 of an Arabidopsis thaliana α-tubulin Causes Extensive Cell Proliferation and Expansion Defects

在线全文

学校读者我要写书评

暂无评论

Journal of Integrative Plant Biology 2013年第3期55卷 209-220页

作者： Xue Xiong Deyang Xu Zhongnan Yang Hai Huang Xiaofeng Cui National Laboratory of Plant Molecular Genetics Shanghai Institute of Plant Physiology and EcologyShanghai Institutes for Biological SciencesChinese Academy of Sciences College of Life and Environmental Sciences Shanghai Normal University

Microtubules are highly dynamic cytoskeletal polymers of α/β-tubulin heterodimers that undergo multiple post-translational modifications essential for various cellular functions in eukaryotes. The lysine 40 （K40） is largely conserved in α-tubulins in many eukaryote species, and the post-translational modification by acetylation at K40 is critical for neuronal development in vertebrates. However, the biological function of K40 of α-tubulins in plants remains unexplored. In this study, we show in Arabidopsis thaliana that constitutive expression of mutated forms of α-tubulin6 （TUA6） at K40 （TUA6K40A or TUA6K40Q ）, in which K40 is replaced by alanine or glutamine, result in severely reduced plant size. Phenotypic characterization of the 35S：TUA6K40A transgenic plants revealed that both cell proliferation and cell expansion were affected. Cytological and biochemical analyses showed that the accumulation of α- and β-tubulin proteins was significantly reduced in the transgenic plants, and the cortical microtubule arrays were severely disrupted, indicating that K40 of the plant α-tubulin is critical in maintaining microtubule stability. We also constructed 35S：TUA6K40R transgenic plants in which K40 of the engineered TUA6 protein is replaced by an arginine, and found that the 35S：TUA6K40R plants were phenotypically indistinguishable from the wild-type. Since lysine and arginine are similar in biochemical nature but arginine cannot be acetylated, these results suggest a structural importance for K40 of α-tubulins in cell division and expansion.

关键词： Arabidopsis α-tubulin expansion cell proliferation microtubule,

C4 Rice-an Ideal Arena for Systems Biology Research

在线全文

学校读者我要写书评

暂无评论

Journal of Integrative Plant Biology 2010年第8期52卷 762-770页

作者： Xin-Guang Zhu Lanlan Shan Yu Wang William Paul Quick Key Laboratory of Computational Biology CAS-MPG Partner Institute for Computational Biology Shanghai Institutes for Biologica Sciences Chinese Academy of Sciences Shanghai 200031 China The International Rice Research Institute Los Banos Philippines

Engineering the C4 photosynthetic pathway into C3 crops has the potential to dramatically increase the yields of major C3 crops. The genetic control of features involved in C4 photosynthesis are still far from being understood; which partially explains why we have gained little success in C4 engineering thus far. Next generation sequencing techniques and other high throughput technologies are offering an unprecedented opportunity to elucidate the developmental and evolutionary processes of C4 photosynthesis. Two contrasting hypotheses about the evolution of C4 photosynthesis exist, i.e. the master switch hypothesis and the incremental gain hypothesis. These two hypotheses demand two different research strategies to proceed in parallel to maximize the success of C4 engineering. In either case, systems biology research will play pivotal roles in identifying key regulatory elements controlling development of C4 features, identifying essential biochemical and anatomical features required to achieve high photosynthetic efficiency, elucidating genetic mechanisms underlining C4 differentiation and ultimately identifying viable routes to engineer C4 rice. As a highly interdisciplinary project, the C4 rice project will have far-reaching impacts on both basic and applied research related to agriculture in the 21st century.

关键词： Engineering Engines Engineers Viverridae Genetics Crops Oesophagus Forecasting Rice Technology

Petal Development in Lotus japonicus

在线全文

学校读者我要写书评

暂无评论

Journal of Integrative Plant Biology 2011年第10期53卷 770-782页

作者： Lin Weng Zhaoxia Tian Xianzhong Feng Xin Li Shilei Xu Xiaohe Hu Da Luo Jun Yang National Key Laboratory of Plant Molecular Genetics Institute of Plant Physiology and Ecology Shanghai Institutes for Biological Sciences Graduate School of the Chinese Academy of Sciences Chinese Academy of Sciences Shanghai 200032 China Key Laboratory of Plant Stress Research of Shandong Province College of Life Sciences Shandong Normal University Jinan 250014 China State Key Laboratory of Biocontrol School of Life Sciences Sun Yat-sen University Guangzhou 510275 China

Previous studies have demonstrated that petal shape and size in legume flowers are determined by two separate mechanisms, dorsoventral （DV） and organ internal （IN） asymmetric mechanisms, respectively. However, little is known about the molecular mechanisms controlling petal development in legumes. To address this question, we investigated petal development along the floral DV axis in Lotus japonicus with respect to cell and developmental biology by comparing wild-type legumes to mutants. Based on morphological markers, the entire course of petal development, from initiation to maturity, was grouped to define 3 phases or 13 stages. In terms of epidermal micromorphology from adaxial surface, mature petals were divided into several distinct domains, and characteristic epidermal cells of each petal differentiated at stage 9, while epidermal cells of all domains were observed until stage 12. TCP and MIXTA-like genes were found to be differentially expressed in various domains of petals at stages 9 and 12. Our results suggest that DV and IN mechanisms interplay at different stages of petal development, and their interaction at the cellular and molecular level guides the elaboration of domains within petals to achieve their ideal shape, and further suggest that TCP genes determine petal identity along the DV axis by regulatincl MIXTA-like clene expression.

关键词： CYC-like TCP genes epidermal cell Lotusjaponicus MIXTA-like genes petal.

Proteomic characteristics of the liver and skeletal muscle in the Chinese tree shrew(Tupaia belangeri chinensis)

在线全文

学校读者我要写书评

暂无评论

Protein & Cell 2012年第9期3卷 691-700页

作者： Rongxia Li Wei Xu Zhen Wang Bin Liang Jia-Rui Wu Rong Zeng Key Laboratory of Systems Biology Institute of Biochemistry and Cell BiologyShanghai Institutes for Biological ScienceChinese Academy of SciencesShanghai 200031China Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences and Yunnan Province Kunming Institute of ZoologyKunming 650223China

Valid animal models are useful for studying the pathophysiology of specific disorders,such as neural disease,diabetes and *** molecular phylogeny studies indicate that the tree shrew is in the same order as(or a close sister to)primates,and thus may be an ideal model in which to study human *** this study,the proteome of liver and muscle tissue in tree the shrew was identified by combining peptide fractionation and LC-MS/MS *** total,2146 proteins were detected,including 1759 proteins in liver samples and 885 proteins in skeletal muscle samples from the tree *** sub-source analysis revealed that nearly half of the identified proteins(846 proteins and 418 proteins)were derived from human *** this study,we are the first to describe the characteristics of the proteome from the liver and skeletal muscle of the tree *** tree analysis based on these proteomic data showed that the tree shrew is closer to primates(human)than to glires(the mouse and rat).

关键词： proteome Chinese tree shrew phylogenetic tree liver muscle