检索结果-南通市图书馆

EDIL3 depletion suppress epithelial-mesenchymal transition of lens epithelial cells via transforming growth factor β pathway

引用

International Journal of Ophthalmology(English edition) 2018年第1期11卷 18-24页

作者： Rui Zhang You-Heng Wei Chun-Yan Zhao Hong-Yuan Song Ni Shen Xiao Cui Xin Gao Zhong-Tian Qi Ming Zhong Wei Shen Department of Ophthalmology Changhai Hospital Second Military Medical University State Key Laboratory of Genetic Engineering Institute of Genetics School of Life Sciences Fudan University Department of Microbiology Second Military Medical University

AIM： To study the effect of discoidin I-like domaincontaining protein 3（EDIL3） depletion on the proliferation and epithelial-mesenchymal transition（EMT） in human lens epithelial cells（LECs）. METHODS： RNA interference was used to inhibit the expression of EDIL3 in human LECs in vitro. The morphology of cells was observed using an inverted microscope. Cell proliferation was assessed using Ed U kit. Cell migration was investigated using Transwell chamber and EMT of LECs was assessed using confocal microscope and Western blotting. The transforming growth factor β（TGFβ） pathway was investigated using Western blotting. RESULTS： The data showed that silencing EDIL3 expression changed LECs morphology and suppressed LECs proliferation（P〈0.05） and migration（P〈0.01）. Furthermore, the result of Western blotting showed that EDIL3 depletion reduced the expression of α-smooth muscle actin（α-SMA）（P〈0.001） and vimentin（P〈0.01）, while increased the expression of E-cadherin（P〈0.001）. EDIL3 depletion could suppress the phosphorylation of Smad2（P〈0.01） and Smad3（P〈0.01） and the activation of exracellular signal regulated kinase（ERK）（P〈0.05）. CONCLUSION： The findings indicate that EDIL3 might participate in the proliferation and EMT in LECs via TGFβ pathway and may be a potential therapeutic target for the treatment of posterior capsule opacification.

关键词： discoidin I-like domain-containing protein 3 transforming growth factor β epithelial-mesenchymal transition human lens epithelial cells

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Inhibition of retinal angiogenesis by gold nanoparticles via inducing autophagy

引用

International Journal of Ophthalmology(English edition) 2018年第8期11卷 1269-1276页

作者： Ni Shen Rui Zhang Hao-Rui Zhang Hao-Yang Luo Wei Shen Xin Gao Da-Zhi Guo Jie Shen Department of Ophthalmology Shanghai Renji HospitalShanghai Jiao Tong University School of Medicine Department of Ophthalmology Changhai Hospitalthe Second Military Medical Univerisity Company 6 of Basic Medical School the Second Military Medical University School of Life Science Fudan University Department of Hyperbaric Oxygen Navy General Hospital

AIM： To investigate the effect of gold nanoparticles on retinal angiogenesis in vitro and in vivo, and to reveal the possible ***： Seed growth method was used to synthesize gold nanoparticles（GNPs）. The size, zeta potential, absorption spectrum and morphology of GNPs were identified using Malvern Nano-ZS, multimode reader（Bio Tek synergy2） and transmission electron microscope. Cell viability was analyzed using cell counting kit-8 method and cell growth was assessed with EdU kit. Transwell chamber was used to investigate cell migration. Tube formation method was used to assess the angiogenic property in vitro. Oxygen induced retinopathy（OIR） model was used to investigate the effect of GNPs on retinal angiogenesis. Confocal microscope and Western blot were used to study the possible mechanism of GNPs inhibited ***： The GNPs synthesized were uniform and well dispersed. GNPs of 10 μg/mL and 20 μg/mL were able to inhibit human umbilical vein endothelial cells proliferation（50% and 72% separately, P〈0.001）, migration（54% and 83% separately, P〈0.001） and tube formation（52% and 90% separately, P〈0.001）. Further data showed that GNPs were able to improve the retinopathy in an OIR model. The possible mechanism might be that GNPs were able to induce autophagy significantly（P〈0.05）.CONCLUSION： The present study suggests that GNPs are able to inhibit retinal neovascularization in vitro and in vivo. GNPs might be a potential nanomedicine for the treatment of retinal angiogenesis.

关键词： gold nanoparticles retinal angiogenesis oxygen induced retinopathy autophagy nanomedicine

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：