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Genome-wide identification of WRKY gene family and expression analysis under abiotic stresses in Andrographis paniculata

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BIOCELL 2021年第4期45卷 1107-1119页

作者： QICHAO WANG WUJING ZENG BASHARAT ALI XUEMIN ZHANG LING XU ZONGSUO LIANG zhejiang province Key Laboratory of Plant Secondary Metabolism and Regulation College of Life Sciences and MedicineZhejiang Sci-Tech UniversityHangzhou310018China Department of Agronomy University of AgricultureFaisalabad38040Pakistan Tianjin Tasly Modern TCM Resources Co. Ltd.Tianjin300410China

Andrographis paniculata(***)is a Chinese herbal medicine that clears away heat,reduces inflammation,protects the liver,and promotes *** WRKYs of *** are still not well characterized,although many WRKYs have been identified in various plant *** the present study,59 *** WRKY(ApWRKY)genes were identified and renamed on the basis of their respective chromosome *** ApWRKYs were divided into three groups via phylogenetic analysis according to their WRKY domains and combined with WRKY of *** 59 identified ApWRKY transcription factors were nonuniformity distributed on 23 chromosomes of *** the structural analysis of the conserved motifs,different ApWRKYs structures showed different biological functions,and the ApWRKY transcription factor had certain species-specificity in the evolutionary *** expression patterns of the 41 ApWRKYs were examined through quantitative real-time PCR(qRT-PCR)in various tissues and under abiotic stresses(salt).The results showed that most of the ApWRKY had different reactions to salt *** addition,the content of the four main secondary metabolites in *** leaves was determined under salt *** results show that under a low concentration of salt treatment,the synthesis of andrographolide can be improved.

关键词： A.paniculata ApWRKY Secondary metabolites Diterpene lactones Andrographolide Abiotic stress

Human cytomegalovirus autophagy is related to the interferon synthesis and mTOR signal pathway

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BIOCELL 2022年第10期46卷 2275-2280页

作者： DONGMEI GAO JIAOE CHEN HONGZHANG LI JUN ZHAO Department of Clinical Laboratory Third Affiliated Hospital of Anhui Medical UniversityHefei230032China Department of Gastroenterology Sanmen People’s HospitalTaizhou317100China Department of Microbiology Anhui Medical UniversityHefei230032China

Introduction:Human cytomegalovirus(HCMV)is reported to be involved in the occurrence of many human *** further investigate the biological changes of HCMV,we analyzed the relevant factors that affect the autophagy caused by HCMV ***:Firstly,we cultured human embryonic lung fibroblasts(HELF)cells with HCMV infection,and evaluated the effects of HELF cells infected with different viruses through Enzyme-linked immunoabsorbent assay(ELISA),Real-time quantitative Polymerase Chain Reaction(RT-qPCR),Acridine orange(AO)staining and Western blotting(WB)***:Through the above experiments,we found that the combined treatment of HCMV infection and carbamazepine,rapamycin and si-mTOR promoted the increase of interferon(IFN)-α/βexpression and protein level,and caused the increase of LC3B protein level in HELF *** addition,HCMV infection could also affect the biological activities of HELF cells by regulating signal pathways like the JAK/***:Autophagy induced by HCMV is affected by the changes in the biological behavior of HELF cells,especially IFN-α/βsynthesis and mTOR signal *** findings might shed new light on HCMVrelated disease treatment.

关键词： HCMV HELF cell mTOR IFN-α IFN-β

A deep learning-based method for pediatric congenital heart disease detection with seven standard views in echocardiography

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World Journal of Pediatric Surgery 2023年第3期6卷 180-187页

作者： Xusheng Jiang Jin Yu Jingjing Ye Weijie Jia Weize Xu Qiang Shu Department of Cardiac Surgery Children's HospitalZhejiang University School of MedicineNational Clinical Research Center for Child HealthHangzhouChina Department of Ultrasound Diagnosis Children’s HospitalZhejiang University School of MedicineNational Clinical Research Center for Child HealthHangzhouChina Innovation Center for Child Health Binjiang Institute of Zhejiang UniversityHangzhouChina

Background With the aggregation of clinical data and the evolution of computational resources,artificial intelligence-based methods have become possible to facilitate clinical *** congenital heart disease(CHD)detection,recent deep learning-based methods tend to achieve classification with few views or even a single *** to the complexity of CHD,the input images for the deep learning model should cover as many anatomical structures of the heart as possible to enhance the accuracy and robustness of the *** this paper,we first propose a deep learning method based on seven views for CHD classification and then validate it with clinical data,the results of which show the competitiveness of our *** A total of 1411 children admitted to the Children’s Hospital of zhejiang University School of Medicine were selected,and their echocardiographic videos were ***,seven standard views were selected from each video,which were used as the input to the deep learning model to obtain the final result after training,validation and *** In the test set,when a reasonable type of image was input,the area under the curve(AUC)value could reach 0.91,and the accuracy could reach 92.3%.During the experiment,shear transformation was used as interference to test the infection resistance of our *** long as appropriate data were input,the above experimental results would not fluctuate obviously even if artificial interference was *** These results indicate that the deep learning model based on the seven standard echocardiographic views can effectively detect CHD in children,and this approach has considerable value in practical application.

关键词： admitted congenital echocardiographic

Terrestrial input and nutrient change reflected by sediment records of the Changjiang River Estuary in recent 80 years

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Acta Oceanologica Sinica 2015年第2期34卷 27-35页

作者： LI Junlong ZHENG Binghui HU Xupeng WANG Yiming DING Ye LIU Fang College of Water Sciences Beijing Normal University State Key Laboratory of Environmental Criteria and Risk Assessment Chinese Research Academy of Environmental Sciences State Environmental Protection Key Laboratory of Quality Control in Environmental Monitoring China National Environmental Monitoring Center Zhoushan Marine Ecological Environmental Monitoring Station

A variety of environmental problems have been observed in the Changjiang River Estuary and adjacent coastal area, including eutrophication, harmful algal blooms（HABs）, and hypoxia in recent decades. Application of sedimentary biogenic element indicators on the study of paleoenvironment can reconstruct environmental evolution history of waters. Two 210Pb-dated cores were collected from the Changjiang River Estuary（S3） and adjacent coastal area（Z13）, and total organic carbon（TOC）, total nitrogen（TN）, biogenic silicon（BSi）, total phosphorus（TP） and phosphorus（P） species were analyzed. Three stages of environmental changes are deduced by the nutrient sedimentary records. First, nutrient concentration increased rapidly since the 1950 s, which attributed to agriculture development and overused chemical fertilizers. Second, nutrient concentration kept high and primary production began to promote during the 1960 s to 1980 s, while diatom abundance and proportion began to decline since the 1970 s, accompanied by reduced 23 Si O- concentration and flux from the river. Third, due to several dams and bridges constructed, river runoff and coastal hydrodynamic conditions reduced to a certain extent since the 1990 s, which aggravated the unbalance in nutrient structure. Multi-nutrient proxies in sediment can reflect the natural environm-ental changes as well as influence of human activities.

关键词： biogenic elements phosphorus species sediment cores Changjiang River Estuary

research and application of the virtual simulation system teaching method in NC machining course

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International Journal of Modeling, Simulation, and Scientific Computing 2018年第1期9卷 168-184页

作者： Yongxiang Li Xiaoping Lu Cong Yu Haixia Guo Dan Zhang Xingzhi College Zhejiang Normal University No.688 Yingbin RoadJinhua Zhejiang 321004P.R.China zhejiang Wanliyang Transmission Co.Ltd No.3999 West Binhong Road JinhuaZhejiang 321025P.R.China College of Economics and Management zhejiang Normal University No.688 Yingbin Road Jinhu Zhejiang 321004P.R.China

Virtual simulation technology has become one of the most popular technologies in the field of engineering education after the multimedia information technology in recent *** paper,based on the comprehensive integrated simulation and verification module of UG NX software,describes and discusses a novel virtual simulation system teaching(VSST)for numerically controlled machining to support the student engineering training to achieve the theoretical knowledge and practical techniques in numerically controlled *** findings of a study designed to evaluate the impact of VSST for the development of numerically controlled machining course are presented *** addition,analysis of the follow-up surveys indicates that the VSST method enables to provide the concrete experience of interaction between the students and the simulation environment and to further stimulate students’interest in learning,so that the students who used VSST achieve significantly higher results than their co-workers.

关键词： Architectures for educational technology systems virtual reality computerbased models and simulations computer-supported learning environment

Putrescine Plays a Positive Role in Salt-Tolerance Mechanisms by Reducing Oxidative Damage in Roots of Vegetable Soybean

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Journal of Integrative Agriculture 2014年第2期13卷 349-357页

作者： ZHANG Gu-wen XU Sheng-chun HU Qi-zan MAO Wei-hua GONG Ya-ming Institute of Vegetable Zhejiang Academy of Agricultural Sciences Center of Analysis and Measurement Zhejiang University

Polyamines play important roles in plant tolerance to environmental stress. With the aim of investigating the possible involvement of putrescine （Put） in salt-tolerance mechanisms in vegetable soybean roots, exogenous Put （10 mmol L＂） and its biosynthetic inhibitor D-arginine （D-Arg）（0.5 mmol L-1） were added to nutrient solution when vegetable soybean （Glycine max L. cv. Huning 95-1） seedlings were exposed to 100 mmol L^-11 sodium chloride （NaCl）. The results showed that Put ameliorated but D-Arg aggravated the detrimental effects of NaCl on plant growth and biomass production. Under NaCl stress, levels of free, soluble conjugated and insoluble bound types of Put in roots of vegetable soybean were reduced, whereas those of free, soluble conjugated, and insoluble bound types of spermidine （Spd） and spermine （Spm） were increased. Exogenous Put eliminated the decrease in Put but promoted the increase of Spd and Spm. However, these changes could be reversed by D-Arg. Under NaCl stress, activities of arginine decarboxylase （ADC）, S-adenosylmethionine decarboxylase （SAMDC）, diamine oxidase （DAO）, and polyamine oxidase （PAO） were induced, with exogenous Put promoting and D-Arg reversing these changes. Furthermore, NaCl stress decreased activities of antioxidant enzymes. Exogenous Put alleviated but D-Arg exaggerated these effects of NaCl stress, resulting in the same changes in membrane damage and reactive oxygen species （ROS） production. These results indicated that Put plays a positive role in vegetable soybean roots by activating antioxidant enzymes and thereby attenuating oxidative damage.

关键词： NaCl stress oxidative damage putrescine salt-tolerance vegetable soybean

Lithium nitrate regulated carbonate electrolytes for practical Li-metal batteries: Mechanisms, principles and strategies

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Journal of Energy Chemistry 2023年第2期77卷 581-600,I0015页

作者： Kun Wang Wenbing Ni Liguang Wang Lu Gan Jing Zhao Zhengwei Wan Wei Jiang Waqar Ahmad Miaomiao Tian Min Ling Jun Chen Chengdu Liang zhejiang Provincial Key Laboratory of Advanced Chemical Engineering Manufacture technology College of Chemical and Biological EngineeringZhejiang UniversityHangzhou 310027ZhejiangChina Institute of zhejiang University-Quzhou Quzhou 324000ZhejiangChina

Li-metal batteries(LMBs)regain research prominence owing to the ever-increasing high-energy *** available carbonate electrolytes exhibit unfavourable parasitic reactions with Limetal anode(LMA),leading to the formation of unstable solid electrolyte interphase(SEI)and the breed of Li dendrites/dead ***,lithium nitrate(LiNO_(3)),an excellent film-forming additive,proves crucial to construct a robust Li_(3)N/Li_(2)O/Li_(x)NO_(y)-rich SEI after combining with ether-based ***,the given challenge leads to natural ideas which suggest the incorporation of LiNO_(3) into commercial carbonate for practical ***,LiNO_(3) demonstrates limited solubility(~800 ppm)in commercial carbonate ***,developing stable SEI and dendrite-free LMA with the incorporation of LiNO_(3) into carbonate electrolytes is an efficacious strategy to realize robust LMBs via a scalable and cost-effective ***,this review unravels the grievances between LMA,LiNO_(3)and carbonate electrolytes,and enables a comprehensive analysis of LMA stabilizing mechanism with LiNO_(3),dissolution principle of LiNO_(3) in carbonate electrolytes,and LiNO_(3) introduction *** review converges attention on a point that the LiNO_(3)-introduction into commercial carbonate electrolytes is an imperious choice to realize practical LMBs with commercial 4 V layered cathode.

关键词： Li-metal battery Carbonate electrolyte Lithium nitrate Stabilization mechanism Dissolution principle Introduction strategy

Metabolic dysfunction is associated with steatosis but no other histologic features in nonalcoholic fatty liver disease

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World Journal of Clinical Cases 2022年第13期10卷 4097-4109页

作者： Yi-Ning Dai Cheng-Fu Xu Hong-Ying Pan Hai-Jun Huang Mei-Juan Chen You-Ming Li Chao-Hui Yu Department of Gastroenterology The First Affiliated HospitalZhejiang University School of MedicineHangzhou 310003Zhejiang ProvinceChina Center for General Practice Medicine Department of Infectious DiseasesZhejiang Provincial People's Hospital(Affiliated People’s HospitalHangzhou Medical College)Hangzhou 310014Zhejiang ProvinceChina

BACKGROUND Recently,nonalcoholic fatty liver disease(NAFLD)has been renamed metabolicassociated fatty liver disease(MAFLD).Based on the definition for MAFLD,a group of non-obese and metabolically healthy individuals with fatty liver are excluded from the newly proposed *** To analyze the histologic features in the MAFLD and non-MAFLD subgroups of *** Eighty-three patients with biopsy-proven NAFLD were separated into MAFLD and non-MAFLD *** diagnosis of MAFLD was established as hepatic steatosis along with obesity/diabetes or evidence of metabolic *** histologic features were compared according to different metabolic disorders and liver enzyme *** MAFLD individuals had a higher NAFLD activity score(P=0.002)and higher severity of hepatic steatosis(42.6%Grade 1,42.6%Grade 2,and 14.8%Grade 3 in MAFLD;81.8%Grade 1,13.6%Grade 2,and 4.5%Grade 3 in non-MAFLD;P=0.007)than the non-MAFLD *** and portal inflammation,hepatic ballooning,fibrosis grade,and the presence of nonalcoholic steatohepatitis(NASH)and significant fibrosis were comparable between the two *** higher the liver enzyme levels,the more severe the grades of hepatic steatosis(75.0%Grade 1 and 25.0%Grade 2 in normal liver function;56.6%Grade 1,39.6%Grade 2,and 3.8%Grade 3 in increased liver enzyme levels;27.8%Grade 1,27.8%Grade 2,and 44.4%Grade 3 in liver injury;P3×upper limit of normal)presented a higher severity of hepatocellular ballooning(P=0.021).Moreover,the grade of steatosis correlated significantly with hepatocellular ballooning degree(r=0.338,P=0.002)and the presence of NASH(r=0.466,Presearch is needed to assess the dynamic histologic characteristics in NAFLD based on the presence or absence of metabolic disorders.

关键词： Nonalcoholic fatty liver disease Metabolic associated fatty liver disease Liver histology Hepatic steatosis Fibrosis

Calculation method of existing tunnel displacement under action of support trolley in overlapping tunnel

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Yantu Lixue/Rock and Soil Mechanics 2020年 41卷 1-10页

作者： Wei, Gang Qi, Yong-Jie Sun, Qiao Wu, Hua-Jun Department of Civil Engineering Zhejiang University City College Hangzhou310015 China School of Civil Engineering and Architecture Anhui University of Science and Technology Huainan232001 China The Architectural Design & research Institute of zhejiang University Co. Ltd. Hangzhou310028 China Yiwu Industrial & Commercial College Yiwu322000 China

The excavation of the upper tunnel in the overlapping section may cause the uplift of the lower tunnel, which is often reinforced by the support trolley. In order to study the influence of the upper shield excavation of the overlap section on the vertical displacement of the lower existing tunnel, the reinforcement and weight of the supporting trolley were comprehensively considered. In view of the uneven rigidity of the existing tunnel along the longitudinal direction, a calculation model of the vertical deformation of the tunnel with the uneven longitudinal rigidity was proposed. Moreover, a calculation formula of the vertical deformation of the existing tunnel was deduced. The influence of different clear distances and different additional loads on the vertical displacement of the tunnel was studied. The research results show that the results obtained by this calculation method are in good agreement with the results obtained by finite element simulation and other scholars. The uplift deformation of the existing tunnel is mainly concentrated in the range of 3.5 times the diameter before and after the excavation, and the length of the influence range is basically unchanged before and after the support trolley is installed, while the overall deformation area moves to the opposite direction of shield tunneling. The supporting trolley has limited effect on controlling the uplift deformation of the existing tunnel, while can effectively control the deformation of its cross section deformation. Moreover, increasing the clear distance can effectively reduce the uplift deformation of the existing tunnel, and reducing the burying depth of the upper shield z0 is more effective than increasing the burial depth h of the existing tunnel. Furthermore, additional weight can help control the deformation (heave or settlement), but too large weight may cause the existing tunnel to sink and deform before and after the excavation. © 2020, Science Press. All right reserved.

关键词： Reinforcement