检索结果-南通市图书馆

Knockdown of HE4 suppresses tumor growth and invasiveness in lung adenocarcinoma through regulation of EGFR signaling

引用

Oncology Research 2024年第6期32卷 1119-1128页

作者： YUE ZHANG WENYU YANG XIAOWANG HAN YUE QIAO HAITAO WANG TING CHEN TIANYING LI WEN-BIN OU Department of Biopharmaceutics College of Life Sciences and MedicineZhejiang Provincial Key Laboratory of Silkworm Bioreactor and BiomedicineZhejiang Sci-Tech UniversityHangzhou310018China Department of Thoracic Surgery Zhejiang Provincial People’s HospitalHangzhou310014China

It has been shown that the high expression of human epididymis protein 4(HE4)in most lung cancers is related to the poor prognosis of patients,but the mechanism of pathological transformation of HE4 in lung cancer is still *** current study is expected to clarify the function and mechanism of HE4 in the occurrence and metastasis of lung adenocarcinoma(LUAD).Immunoblotting evaluated HE4 expression in lung cancer cell lines and biopsies,and through analysis of The Cancer Genome Atlas(TCGA)*** HE4 overexpression was demonstrated in LUAD,but not in lung squamous cell carcinoma(LUSC),indicating that HE4 can serve as a biomarker to distinguish between LUAD and ***4 knockdown significantly inhibited cell growth,colony formation,wound healing,and invasion,and blocked the G1-phase of the cell cycle in LUAD cell lines through inactivation of the EGFR signaling downstream including PI3K/AKT/mTOR and RAF/MAPK *** first-line EGFR inhibitor gefitinib and HE4 shRNA had no synergistic inhibitory effect on the growth of lung adenocarcinoma cells,while the third-line EGFR inhibitor osimertinib showed additive anti-proliferative ***,we provided evidence that HE4 regulated EGFR expression by transcription regulation and protein interaction in *** findings suggest that HE4 positively modulates the EGFR signaling pathway to promote growth and invasiveness in LUAD and highlight that targeting HE4 could be a novel strategy for LUAD treatment.

关键词： Lung adenocarcinoma Human epididymis protein 4 Epidermal growth factor receptor Biomarker Targeted therapies

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

An antifouling polydopamine-based fluorescent aptasensor for determination of arginine kinase

引用

Food Science and Human Wellness 2023年第3期12卷 737-744页

作者： Yanbo Wang Huan Li Jinru Zhou Fangting Wang Yifan Qian Linglin Fu Food Safety Key Laboratory of Zhejiang Province School of Food Science and BiotechnologyZhejiang Gongshang UniversityHangzhou 310018China

Simple yet efficient detection methods for food allergens are in urgent need to help people avoid the risks imposed by allergenic *** this work,a polydopamine(PDA)-based fluorescent aptasensor was developed to detect arginine kinase(AK),one of the major allergens in *** aptamer towards AK was firstly selected via systematic evolution of ligands by exponential enrichment method and labeled with fluorescein amidite(FAM)to build a fluorescence resonance energy transfer(FRET)system with PDA *** glycol(PEG)was employed to construct an antifouling surface for the aptasensor to eliminate food matrix *** the presence of AK,the PDA-based aptasensor exhibited elevated fluorescent signals as the FAM-labeled aptamer bound to AK and detached from the PDA *** aptasensor showed great stability and resistance to nonspecific interference of background proteins and had a limit of detection(LOD)of 0.298μg/*** proposed aptasensor was further proved to be feasible for quantitative analysis of AK in nine species of shrimps and five commercial processed products,which indicated its high potential in tracing the presence of AK in complex aquatic products.

关键词： Food allergy Aptasensor Antifouling Polydopamine FRET effect

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Speckle-based interrogation system for quasi-distributed weak fiber Bragg gratings

引用

Chinese Optics Letters 2023年第12期21卷 81-86页

作者：陶缙超梁芹李玥孟彦龙裘燕青周鹏威赵春柳康娟张先超蒋治国王乐李裔 College of Optical and Electronic Technology China Jiliang UniversityHangzhou 310018China Key Laboratory of Medical Electronics and Digital Health of Zhejiang Province Jiaxing UniversityJiaxing 314001China

A simple quasi-distributed fiber sensing interrogation system based on random speckles is proposed for weak fiber Bragg gratings(WFBGs) in this work. Without using tunable lasers or spectrometers, a piece of multimode fiber is applied to interrogate the WFBGs relying on the wavelength sensitivity of speckles. Instead of the CCD sensor, an InGaAs quadrant detector serves as the receiver to capture the fast-changing speckle patterns. A supervised deep learning algorithm of the multilayer perceptron architecture is implemented to process speckle data and to interrogate temperature changes or dynamic strains. The proposed demodulation system is experimentally demonstrated for WFBGs with 0.1% *** experimental results demonstrate that the new system is capable of measuring temperature change with an accuracy of 1℃ and achieving dynamic frequency of 100 Hz. This speckle-based interrogation system paves a new way for distributed WFBGs sensing with a simple design.

关键词： weak fiber Bragg gratings random speckles deep learning time-division-multiplexing

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Resetting amino acid metabolism of cancer cells by ATB^(0,+)-targeted nanoparticles for enhanced anticancer therapy

引用

Bioactive Materials 2022年第3期7卷 15-28页

作者： Longfa Kou Xinyu Jiang Yingying Tang Xing Xia Yingtao Li Aimin Cai Hailun Zheng Hailin Zhang Vadivel Ganapathy Qing Yao Ruijie Chen Department of Pharmacy The Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical UniversityWenzhou325027China School of Pharmaceutical Sciences Wenzhou Medical UniversityWenzhou325035China Department of Children’s Respiration Disease The Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical UniversityWenzhou325027China Department of Cell Biology and Biochemistry Texas Tech University Health Sciences CenterLubbockTX79430USA

Reprogramed cellular metabolism is one of the most significant hallmarks of *** cancer cells exhibit increased demand for specific amino acids,and become dependent on either an exogenous supply or upregulated de novo *** resultant enhanced availability of amino acids supports the reprogramed metabolic pathways and fuels the malignant growth and metastasis of cancers by providing energy and critical metabolic intermediates,facilitating anabolism,and activating signaling networks related to cell proliferation and ***,pharmacologic blockade of amino acid entry into cancer cells is likely to have a detrimental effect on cancer cell *** we developed a nanoplatform(LJ@Trp-NPs)to therapeutically target two transporters,SLC6A14(ATB^(0,+))and SLC7A5(LAT1),that are known to be essential for the sustenance of amino acid metabolism in most *** LJ@Trp-NPs uses tryptophan to guide SLC6A14-targeted delivery of JPH203,a high-affinity inhibitor of *** the process,SLC6A14 is also *** tested the ability of this strategy to synergize with the anticancer efficacy of lapatinib,an inhibitor of EGFR/HER1/HER2-assocated *** studies show that blockade of amino acid entry amplifies the anticancer effect of lapatinib via interference with mTOR signaling,promotion of apoptosis,and suppression of cell proliferation and *** represents the first study to evaluate the impact of amino acid starvation on the anticancer efficacy of widely used kinase inhibitor.

关键词： Amino acid delivery ATB^(0,+)targeting Nanoparticles Cancer therapy mTOR

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Chickpea C2H2-Type Zinc Finger Protein ZF2 is a Positive Regulator in Drought Response in Arabidopsis

引用

Phyton-International Journal of Experimental Botany 2023年第2期92卷 577-590页

作者： Sushuang Liu Yanmin Liu Chundong Liu Xingwang Yu Hao Ma Department of Life Sciences and Health Huzhou CollegeHuzhou313000China State Key Laboratory of Crop Genetics and Germplasm Enhancement Nanjing Agricultural UniversityNanjing210095China Department of Crop and Soil Sciences North Carolina State UniversityRaleigh27695-7620USA Institute of Desert in the Arid Areas College of Grassland and Environment SciencesXinjiang Agricultural UniversityUrumqi830052China

Drought is a major abiotic stress limiting agricultural crops production *** our study,we isolated a novel C2H2-type zinc finger protein gene ZF2 from ***2 consisted of 232 amino acids with two QALGGH motifs in Cys2/His2 zinc finger *** expression analysis of ZF2:GFP fusion protein showed that ZF2 was a nuclear localized *** the yeast assay system,the full-length of ZF2 did not show transcriptional *** of ZF2 gene was enhanced by treatments of several abiotic stresses and *** promoter region of ZF2 contained multiple stress-and hormone-related *** of ZF2 in Arabidopsis significantly improved the root length and fresh weight at seedling stage and enhanced the survival rates and proline content under drought *** results indicated that ZF2 functioned as a positive regulator in drought response.

关键词： ZF2 chickpea drought tolerance transgenic

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

A reciprocating motion-driven rotation mechanism for the ATP synthase

引用

Science China(Life Sciences) 2016年第1期59卷 44-48页

作者： Jiafeng Liu xinmiao Fu Zengyi Chang State Key Laboratory of Protein and Plant Gene Research School of Life Sciences Peking University Center for Protein Science Peking University

The ATP synthase(having a typical subunit composition of ?3?3???ab2c8-15) employs an intriguing rotary mechanism for the generation of ATP from ADP and Pi, using energy stored in a transmembrane proton gradient. The conventional rotary model, although being generally accepted, remains difficult to explain certain experimental observations. Here we propose an alternative rotary model for the ATP synthase such that what rotates is the catalytic ?3?3 cylinder rather than the central stalk and the membrane-embedded c-ring. Specifically, the membrane translocation of protons would induce a cycled conformational change in the c-ring, leading to a reciprocating motion of the attached central stalk, which in turn drives the unidirectional rotation of the ?3?3 cylinder. Such a reciprocating motion-driven rotation mechanism is somehow analogous to the working mechanism of a retractable click ballpoint pen. Our new model not only explains the experimental observations that have been difficult to reconcile with the conventional model but also avoids its theoretical illogicality.

关键词：旋转机构往复运动旋转机械驱动 ATP合成酶旋转模型质子梯度实验观察

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

3-epi-bufotalin suppresses the proliferation in colorectal cancer cells through the inhibition of the JAK1/STAT3 signaling pathway

引用

BIOCELL 2022年第11期46卷 2425-2432页

作者： SANHUA LI QINGHONG KONG XIAOKE ZHANG XINTING ZHU CHUNBO YU CHANGYAN YU NIAN JIANG JING HUI LINGJIE MENG YUN LIU Guizhou Provincial College–Based Key Lab for Tumor Prevention and Treatment with Distinctive Medicines Zunyi563000China Life Science Institute of Zunyi Medical University Zunyi563000China School of Preclinical Medicine of Zunyi Medical University Zunyi563000China

Traditional Chinese medicine(TCM)has been increasingly employed in the last decades in China for both preventing and treating a variety of cancers.3-epi-bufotalin is an active ingredient of TCM“Chanpi”with anti-tumor ***,the effect and mechanism of 3-epi-bufotalin on colorectal cancers were not well *** present study demonstrated that 3-epi-bufotalin could reduce viability,trigger apoptosis,and block the cell cycle at the G2/M stage in colorectal cancer cell lines HT29,RKO,and COLO205 in ***,3-epi-bufotalin inhibited the JAK1/STAT3 signaling *** results indicated the anti-proliferation ability of 3-epi-bufotalin in colorectal cancer cells.

关键词： 3-epi-bufotalin Colorectal cancer JAK1/STAT3 signaling pathway Apoptosis

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Multi-objective Trajectory Planning Method based on the Improved Elitist Non-dominated Sorting Genetic Algorithm

引用

Chinese Journal of Mechanical Engineering 2022年第1期35卷 70-84页

作者： Zesheng Wang Yanbiao Li Kun Shuai Wentao Zhu Bo Chen Ke Chen College of Mechanical Engineering Zhejiang University of TechnologyHangzhou 310023China Key Laboratory of E&M Ministry of Education&Zhejiang ProvinceZhejiang University of TechnologyHangzhou 310032China

Robot manipulators perform a point-point task under kinematic and dynamic *** to multi-degreeof-freedom coupling characteristics,it is difficult to find a better desired *** this paper,a multi-objective trajectory planning approach based on an improved elitist non-dominated sorting genetic algorithm(INSGA-II)is *** function is planned with a new composite polynomial that by combining of quintic polynomials with cubic Bezier ***,an INSGA-II,by introducing three genetic operators:ranking group selection(RGS),direction-based crossover(DBX)and adaptive precision-controllable mutation(APCM),is developed to optimize travelling time and torque *** generational distance,hypervolume and optimizer overhead are selected to evaluate the convergence,diversity and computational effort of *** optimal solution is determined via fuzzy comprehensive evaluation to obtain the optimal *** a serial-parallel hybrid manipulator as instance,the velocity and acceleration profiles obtained using this composite polynomial are compared with those obtained using a quintic B-spline *** effectiveness and practicability of the proposed method are verified by simulation *** research proposes a trajectory optimization method which can offer a better solution with efficiency and stability for a point-to-point task of robot manipulators.

关键词： Hybrid manipulator Bezier curve Improved optimization algorithm Trajectory planning Multi-objective optimization

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Facile synthesis of indoles by K2CO3 catalyzed cyclization reaction of 2-ethynylanilines in water

引用

Chinese Chemical Letters 2017年第2期28卷 231-234页

作者： Zhi Chen Xiao-Xiao Shi Dong-Qin Ge Zhen-Zhen Jiang Qi-Qi Jin Hua-Jiang Jiang Jia-Shou Wu School of Pharmaceutical and Chemical Engineering Taizhou University Taizhou 318000 China

The cyclization reaction of 2-ethynyl-N-sulfonylanilides proceeded efficiently in water with the presence of a catalytic amount of K2CO3 under transition metal-free condition to give indoles in high *** recovery and r... 详细信息

关键词： 2-Ethynylanilines Cyclization reaction Indoles Water Potassium carbonate

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

A comparative study of formaldehyde and carbon monoxide complete oxidation on MnO_x-CeO_2 catalysts

引用

Journal of Rare Earths 2009年第3期27卷 419-424页

作者：刘雪松鲁继青千坤黄伟新罗孟飞 Zhejiang Key Laboratory for Reactive Chemistry on Solid Surfaces Institute of Physical ChemistryZhejiang Normal University National Laboratory for Physical Sciences at the Microscale and Department of Chemical Physics University of Science and Technology of China

MnOx-CeO2 composite catalysts were prepared by a coprecipitation method and tested for formaldehyde （HCHO） and carbon monoxide （CO） oxidation. X-ray photon spectroscopy （XPS） results indicated that the average oxidation state of surface Mn species in CeMn composite catalyst was higher compared to the pure MnOx. The enhancement of reactivity for HCHO oxidation was due to the activation of the lattice oxygen species in MnOx by the addition of CeO2, which was confirmed by the H2 temperature programmed reduction （HE-TPR） results. The remarkable enhancement of reactivity for CO oxidation by the addition of CeO2 was due to the active oxygen species generated on the CeO2 surface which directly participated in the reaction.

关键词： MnOx-CeO2 catalysts complete oxidation HCHO CO rare earths

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：