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The Acoustic Attenuation Prediction for seafloor sediment Based on in-situ Data and Machine Learning Methods

Journal of Ocean University of China 2025年第1期24卷 95-102页

作者： WANG Jingqiang HOU Zhengyu CHEN Yinglin LI Guanbao KAN Guangming XIAO Peng LI Zhenglin MO Dinghao HUANG Jingyi Key laboratory of marine Geology and Metallogeny First Institute of OceanographyMNRQingdao 266061China school of Ocean Engineering and Technology Sun Yat-Sen UniversityZhuhai 519000China laboratory for marine Geology Laoshan LaboratoryQingdao 266237China

Accurate acquisition and prediction of acoustic parameters of seabed sediments are crucial in marine sound propagation *** the relationship between sound velocity and physical properties of sediment has been extensively studied,there is still no consensus on the correlation between acoustic attenuation coefficient and sediment physical *** the acoustic attenuation coefficient remains a challenging issue in sedimentary acoustic *** this study,we propose a prediction method for the acoustic attenuation coefficient using machine learning algorithms,specifically the random forest(RF),support vector machine(sVR),and convolutional neural network(CNN)*** utilized the acoustic attenuation coefficient and sediment particle size data from 52 stations as training parameters,with the particle size parameters as the input feature matrix,and measured acoustic attenuation as the training label to validate the attenuation prediction *** results indicate that the error of the attenuation prediction model is *** the three models,the RF model exhibited the lowest prediction error,with a mean squared error of 0.8232,mean absolute error of 0.6613,and root mean squared error of ***,when we applied the models to predict the data collected at different times in the same region,we found that the models developed in this study also demonstrated a certain level of reliability in real prediction *** approach demonstrates that constructing a sediment acoustic characteristics model based on machine learning is feasible to a certain extent and offers a novel perspective for studying sediment acoustic properties.

关键词： in-situ measurement attenuation seafloor sediment machine learning methods

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Functional Inferences of Environmental Coccolithovirus Biodiversity

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Virologica sinica 2013年第5期28卷 291-302页

作者： Jozef I Nissimov Mark Jones Johnathan A Napier Colin B Munn susan A Kimmance Michael J Allen plymouth marine laboratory Prospect Place University of Nottingham School of BiosciencesSutton Bonington Campus Department of Biological Chemistry Rothamsted Research school of marine science & Engineering Plymouth University

The cosmopolitan calcifying alga Emiliania huxleyi is one of the most abundant bloom forming coccolithophore species in the oceans and plays an important role in global biogeochemical cycling. Coccolithoviruses are a major cause of coccolithophore bloom termination and have been studied in laboratory, mesocosm and open ocean studies. However, little is known about the dynamic interactions between the host and its viruses, and less is known about the natural diversity and role of functionally important genes within natural coccolithovirus communities. Here, we investigate the temporal and spatial distribution of coccolithoviruses by the use of molecular fingerprinting techniques PCR, DGGE and genomie sequencing. The natural biodiversity of the virus genes encoding the major capsid protein （MCP） and serine palmitoyltransferase （sPT） were analysed in samples obtained from the Atlantic Meridional Transect （AMT）, the North sea and the L4 site in the Westem Channel Observatory. We discovered nine new coccolithovirus genotypes across the AMT and L4 site, with the majority of MCP sequences observed at the deep chlorophyll maximum layer of the sampled sites on the transect. We also found four new sPT gene variations in the North sea and at L4. Their translated fragments and the full protein sequence of sPT from laboratory strains EhV-86 and EhV-99B 1 were modelled and revealed that the theoretical fold differs among strains. Variation identified in the structural distance between the two domains of the sPT protein may have an impact on the catalytic capabilities of its active site. In summary, the combined use of ＇standard＇ markers （i.e. MCP）, in combination with metabolically relevant markers （i.e. sPT） are useful in the study of the phylogeny and functional biodiversity of coccolithoviruses, and can provide an interesting intracellular insight into the evolution of these viruses and their ability to infect and replicate within their algal hosts.

关键词： Coccolithovirus Major capsid protein serine palmitoyltransferase Functional biodiversity

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Effects of temperature on the respiratory metabolism,feeding and expression of three heat shock protein genes in Anadara broughtonii

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Journal of Oceanology and Limnology 2021年第2期39卷 755-769页

作者： Xuxu LIU Xiumei ZHANG Yihang WANG Key laboratory of Mariculture Ministry of EducationOcean University of ChinaQingdao 266003China Fisheries College Zhejiang Ocean UniversityZhoushan 316022China laboratory for marine Fisheries science and Food Production Processes Qingdao National Laboratory for Marine Science and TechnologyQingdao 266237China

Anadara broughtonii is one of the main commercially important species of shellfish in northern *** lives in relatively stable subtidal zone where the clam could respond to environmental changes with minimum ***,slight fluctuations in water environment may have a great impact on physiological processes such as feeding and ***-scale mortality often occurs during the intermediate rearing processes,and high temperatures in summer are considered the leading cause of *** understand the physiological and molecular response patterns of *** at high temperatures and to estimate the appropriate metabolism temperature for ***,the effects of temperature on the respiratory metabolism and food intake at different growth stages were *** response patterns of heat shock protein genes to heat stress and post-stress recovery were also *** show that the temperature and growth stage of *** were two important factors affecting metabolism and *** optimum temperature for feeding and physiological activities in each shell-length group was 24℃.The temperature at which the peak metabolic rate occurred in each shell-length group increased with the increase in shell *** the increase in heat stress,the expression of three heat shock protein genes(Abhsp60,Abhsp70,and Abhsp90)in the tissues of two size groups of *** increased significantly when temperature was above 24℃and reached a peak at 30℃.After heat shock at 30℃for 2 h,the expression of the three heat shock protein genes rapidly increased,peaked at 2 h after the heat shock,and then gradually decreased to the level of the control group at 48 h after the heat *** three heat shock protein genes respond rapidly to heat stress and can be used as indicators to the cellular stress response in *** under an environmental stress.

关键词： Anadara broughtonii temperature oxygen consumption rate clearance rate heat shock protein gene

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Development of chloroplast marker for identification of Ulva species

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Journal of Oceanology and Limnology 2022年第6期40卷 2364-2371页

作者： Dahai GAO Qingchun ZHANG Zhongmin sUN Key laboratory of Exploration and Utilization of Aquatic Resources Ministry of EducationShanghai Ocean UniversityShanghai 201306China shanghai Engineering research Center of Aquaculture Shanghai Ocean UniversityShanghai 201306China Department of marine Organism Taxonomy and Phylogeny Institute of OceanologyChinese Academy of Sciences(IOCAS)Qingdao 266071China Key laboratory of marine Ecology and Environmental sciences Institute of OceanologyChinese Academy of SciencesQingdao 266071China

Large-scale Ulva-caused green tides has posed various environmental and ecological problems as well as economic *** is important to identify them accurately and *** current universal markers based on ITs,rbc L,and 5s spacer sequences were defective to distinct closely related species in the genus of *** this study,by investigating the intergenic regions of chloroplast(cp)genome,a novel marker of c15(based on ycf 3-rps 7)were reliable for discrimination of Ulva ***,the resolution of c15 was suitable for resolve the closely related species of *** and ***,which may provide tools for deciphering the blooming mechanisms of green tides.

关键词： Ulva linza-procera-prolifera(LPP)complex molecular makers ycf 3-rps 7 region

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Assessing the effects of oyster/kelp weight ratio on water column properties: an experimental IMTA study at sanggou Bay, China

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Journal of Oceanology and Limnology 2020年第6期38卷 1914-1924页

作者： FANG Jinghui FANG Jianguang CHEN Qionglin MAO Yuze JIANG Zengjie DU Meirong GAO Yaping LIN Fan laboratory for marine Fisheries science and Food Production Processes Pilot National Laboratory for Marine Science and Technology(Qingdao)/Yellow Sea Fisheries Research InstituteChinese Academy of Fishery SciencesQingdao 266071China Key laboratory of marine Biotechnology of Zhejiang Province School of Marine SciencesNingbo UniversityNingbo 315211China

Integrated Multi-Trophic Aquaculture(IMTA)is an effective method for sustainable aquaculture as species from different trophic levels could reduce negative effects from fed species in the environment.A proper proportion of different trophic species in an IMTA system could improve the aquaculture production and environmental *** present,research on the proper proportions for farming species is *** investigated the effects of IMTA modes of oyster(Crassostrea gigas)and kelp(saccharina japonica)in different weight ratios on water quality and carbonate system in a closed enclosure experiment for three days in the sanggou Bay in shandong Province,China,in December *** collocation modes in oyster:kelp weight ratio were tested showing as 24:3,24:2,24:1,16:3,16:2,16:1,8:3,8:2,and 8:*** water parameters were determined at 17:00 on Day 1(D1),and 6:00 and 17:00 on Days 2(D2)and 3(D3).As two-way ANOVA showed,all increased parameters(dissolved oxygen(DO),pH,chl a,the carbonate system and pCO2)were significantly related to oyster-kelp modes,and interaction between modes and time were also significant(Ps the highest in DO,pH,chl a,CO32-(Pssolved inorganic carbon(DIC),HCO3-,CO2,and pCO2 were the lowest(Ps references and the results of this study,the appropriate oyster:kelp proportion at the beginning of winter is from 8:2 to 8:*** results of this study may help government to optimize the aquaculture structure of sanggou Bay.

关键词： Integrated Multi-Trophic Aquaculture(IMTA) Pacific oyster Crassostrea gigas kelp saccharina japonica

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Performance evaluation of fixed-station sampling design for a fishery-independent survey with multiple objectives

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Acta Oceanologica sinica 2022年第3期41卷 94-102页

作者： Jing Wang Binduo Xu Ying Xue Chongliang Zhang Mingkun Li Yiping Ren school of Fishery Zhejiang Ocean UniversityZhoushan 316000China Fisheries College Ocean University of ChinaQingdao 266003China laboratory for marine Fisheries science and Food Production Processes Pilot National Laboratory for Marine Science and Technology(Qingdao)Qingdao 266237China

Fixed-station sampling design was widely used in fishery-independent surveys because of its characteristics of convenient sampling station setting,but the non-probabilistic(fixed)nature made it more uncertainty of drawing inferences on *** performance of fixed-station sampling design for multispecies survey has not been evaluated,and we are uncertain if the design could detect the temporal trends of different populations in multispecies fishery-independent *** this study,spatial distribution of abundance indices for three species with different spatial distribution patterns including small yellow croaker(Larimichthys polyactis),whitespotted conger(Conger myriaster)and Fang’s blenny(Enedrias fangi)were simulated using ordinary kriging interpolation as the“true”population *** performance of fixed-station sampling design was compared with simple random sampling design by resampling the simulated“true”populations in this simulation *** results showed that the fixed-station sampling design had the power to detect the seasonal trends of species *** effectiveness of fixed-station sampling design were different in different species distribution *** the species had even distribution,fixed-station sampling design could get high quality abundance data;when the distribution was uneven with heterogeneity or patchiness,fixed-station sampling design tended to underestimate or overestimate the ***,the estimates of abundance index based on the fixedstation sampling design must be calibrated cautiously while applying them for fisheries stock assessment and *** study suggested that fixed-station sampling design could catch the temporal dynamics of population abundance,but the abundance estimates from the fixed-station sampling design could not be treated as the absolute estimates of populations.

关键词： computer simulation fixed-station sampling design precision and accuracy ordinary kriging interpolation

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Global air-sea CO_(2) exchange fl ux since 1980s: results from CMIP6 Earth system Models

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Journal of Oceanology and Limnology 2022年第4期40卷 1417-1436页

作者： Baoxiao QU Jinming sONG Xuegang LI Huamao YUAN Kun ZHANG suqing XU Key laboratory of marine Ecology and Environmental sciences Institute of OceanologyChinese Academy of SciencesQingdao 266071China marine Ecology and Environmental sciences laboratory Pilot National Laboratory for Marine Science and Technology(Qingdao)Qingdao 266237China University of Chinese Academy of sciences Beijing 100049China Center for Ocean Mega-science Chinese Academy of SciencesQingdao 266071China Key laboratory of Ocean Circulation and Waves Institute of OceanologyChinese Academy of SciencesQingdao 266071China Key laboratory of Global Change and marine-Atmospheric Chemistry Third Institute of OceanographyMinistry of Natural ResourcesXiamen 361005China

The ocean could profoundly modulate the ever-increasing atmospheric CO_(2) by air-sea CO_(2) exchange process,which is also able to cause signifi cant changes of physical and biogeochemical properties in *** this study,we assessed the long-term average and spatial-temporal variability of global air-sea CO_(2) exchange fl ux(F CO_(2))since 1980s basing on the results of 18 Coupled Model Intercomparison Project Phase 6(CMIP6)Earth system Models(EsMs).Our fi ndings indicate that the CMIP6 EsMs simulated global CO_(2) sink in recent three decades ranges from 1.80 to 2.24 Pg C/a,which is coincidence with the results of cotemporaneous ***’s more,the CMIP6 EsMs consistently show that the global oceanic CO_(2) sink has gradually intensifi ed since 1980s as well as the *** study confi rms the simulated F CO_(2) could reach agreements with the observations in the aspect of primary climatological characteristics,however,the simulation skills of CIMP6 EsMs in diverse open-sea biomes are *** of the 18 CMIP6 EsMs could reproduce the observed F CO_(2) increasement in the central-eastern tropical Pacifi c and the midlatitude southern *** ciencies of some CMIP6 EsMs in reproducing the atmospheric pressure systems of the southern Hemisphere and the El Niño-southern Oscillation(ENsO)mode of the tropical Pacifi c are probably the major causes.

关键词： air-sea CO_(2)fl ux Coupled Model Intercomparison Project Phase 6(CMIP6) Earth system Model(EsM) long-term average spatial-temporal variability

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Tracing the settlement region of massive floating green algae in the Yellow sea

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Journal of Oceanology and Limnology 2019年第5期37卷 1555-1565页

作者： GENG Huixia YU Rencheng ZHANG Qingchun YAN Tian KONG Fanzhou ZHOU Mingjiang Key laboratory of marine Ecology and Environmental sciences Institute of OceanologyChinese Academy of SciencesQingdao 266071China laboratory for marine Ecology and Environmental science Pilot National Laboratory for Marine Science and Technology (Qingdao)Qingdao 266237China University of Chinese Academy of sciences Beijing 100049China Center for Ocean Mega-science Chinese Academy of SciencesQingdao 266071China

Outbreaks of large-scale green tides formed by Ulva prolifera in the Yellow sea (Ys) cause heavy ecological damages and huge economic losses. However, the ecological consequences of green tides remain poorly understood due to the lack of knowledge on the settlement region of massive green algae floating in the Ys. In this study, we established a method to trace the settlement region of floating green algae, using 28-isofucosterol preserved in sediment as the specific biomarker for green algae. sterols including 28-isofucosterol in surface sediment samples collected during an investigation in the Ys and the Bohai sea (Bs) in August 2015 were analyzed using gas chromatography coupled with a mass spectrometer (GC-Ms). Based on the content of 28-isofucosterol in sediment samples, the potential settlement region of fl oating green algae was identifi ed in the sea area southeast to the shandong Peninsula around the sampling site H06 (122.66°E, 36.00°N). This paper presents a first result on the settlement region of floating green algae in the Ys for providing a solid basis to elucidate the ecological consequences of green tides in the area.

关键词： green tide biomarker sterol Ulva prolifera

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Experimental clearance rates of Aurelia coerulea ephyrae and medusae,and the predation impact on zooplankton in Jiaozhou Bay

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Journal of Oceanology and Limnology 2020年第4期38卷 1256-1269页

作者： WANG Pengpeng ZHANG Fang sUN song WANG Weicheng WAN Aiyong LI Chaolun CAs Key laboratory of marine Ecology and Environmental sciences Institute of OceanologyChinese Academy of SciencesQingdao 266071China laboratory for marine Ecology and Environmental sciences Qingdao National Laboratory for Marine Science and TechnologyQingdao 266071China University of Chinese Academy of sciences Beijing 100049China Jiaozhou Bay marine Ecosystem research station Chinese Academy of SciencesQingdao 266071China Center for Ocean Mega-science Chinese Academy of SciencesQingdao 266071China

The population explosion of jellyfish Aurelia coerulea occurred in Jiaozhou Bay,China in *** potential predation impact of *** on zooplankton was *** clearance potential and residence time(t 1/2)for copepods were calculated from laboratory clearance rates and measurements of jellyfish size and abundance from May to August 2009 in Jiaozhou *** rates varied widely with prey organisms,but they were not significantly different among various prey *** captured rotifers,fish larvae and hydromedusae more efficiently than fish eggs,copepods and *** captured rotifers and hydromedusae more efficiently than fish larvae and *** rate linearly increased with the cross sectional area of ***(size from 0.3 to 7.1 cm).Water temperature also had a marked effect on clearance rate and this was related to the increased beat frequency as water temperature *** early May 2009,*** potentially cleared the volume of water in the Bay less than 0.001 times a day,but this value was estimated to be more than 0.3 times a day in *** t 1/2 for copepods was less than 6 d in June and *** of copepods,hydromedusae and chaetognaths were extremely low in 2009 compared to 2008 and 2010(jellyfish non-bloom years).Large predation pressure by the *** population occurred to control zooplankton communities in Jiaozhou ***,when present at a high population level,can be a keystone species in Jiaozhou Bay and control the trophic structure here.

关键词： scyphomedusae predation pressure prey selection zooplankton control jellyfish bloom

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Isolation and Neuroprotective Activity of Phenolic Derivatives from the marine-Derived Fungus Penicillium janthinellum

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Journal of Ocean University of China 2020年第3期19卷 700-706页

作者： ZHENG Yaoyao CHEN Xu CHEN Lumin sHEN Li FU Xiumei CHEN Qiuxia CHEN Min WANG Changyun marine science&Technology Institute College of Environmental Science&EngineeringYangzhou UniversityYangzhou 225127China Key laboratory of marine Drugs of Ministry of Education of China School of Medicine and PharmacyOcean University of ChinaQingdao 266003China laboratory for marine Drugs and Bioproducts Qingdao National Laboratory for Marine Science and TechnologyQingdao 266237China Institute of Translational Medicine Medical CollegeYangzhou UniversityYangzhou 225001China Institute of Evolution&marine Biodiversity Ocean University of ChinaQingdao 266003China

A new phenolic compound, 6-(2-acetyl-3,5-dihydroxybenzyl)-4-hydroxy-3-methyl-2H-pyran-2-one(1), along with other six known phenolic derivatives(2-7), were isolated from the mangrove rhizosphere fungus Penicillium janthinellum HK1-6 cultured in potato dextrose broth medium containing 30 g L^(-1) of natural sea salt. The structure of the new compound(1) was elucidated by comprehensive analysis of spectroscopic data including 1D and 2D NMR spectra. The proposed biosynthetic pathway of compound 1 was also studied in this research. Interestingly, a brominated phenolic derivative, aryl bromide(compound 8), was obtained from this fungal strain cultured in medium containing 30 g L^-1 of NaBr instead of natural sea salt. Compound 8 is proposed as a new natural product and formed through bromination of compound 7 when the fungus was cultured with NaBr. The neuroprotective effect of compound 1 on oxygen-glucose deprivation(OGD)-induced injury was investigated in rat spinal cord astrocytes. MTT assay demonstrated that compound 1 can attenuate OGD-induced cell viability loss in rat spinal cord astrocytes.

关键词： marine fungus Penicillium janthinellum phenolic derivative neuroprotective activity

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