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Studies of Transgenic Hybrid Poplar 741 Carrying Two Insect-resistant Genes

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Acta Botanica Sinica 2000年第3期42卷 263-268页

作者：田颖川郑均宝虞红梅梁海永李常青王进茂中国科学院微生物研究所北京100080 河北农业大学保定071000

Partially modified Bt Cry1Ac gene and the arrowhead proteinase inhibitor (API) gene were used to construct a plant transformation vector pBtiA and this construct was transferred into the genome of the hybrid poplar 741 [ Populus alba L.×( P. davidiana Dode+ P. simonii Carr.)× P. tomentosa Carr.] by Agrobacterium _ mediated transformation. Ten kanamycin resistant plants have been regenerated. Upon insect bioassay using Clostera anachoreta (Fabricius), three of the examined plants were demonstrated to be highly resistant to the testing insects. The mortality of insect larvae on one plant was higher than 90% in 6 days after infestation and the growth of the survival larvae were seriously inhibited. Results of PCR and Southern blot analysis indicated that both Bt Cry1Ac gene and API gene were integrated as a single copy into the genomes of these three plants when Cry1Ac gene fragment was used as the probe. Protein dot blot immunoassay and ELISA analysis revealed that at least the Cry1Ac protein was produced in these three transgenic plants and the expression levels were estimated to be approximately 0.015% of the leaf total soluble protein. This is the first report on insect resistant transgenic hybrid poplar 741 that expresses two insecticidal protein genes.

关键词： poplar 741 divalent insect resistant genes transformation

Novel Optical Fiber Gas Sensor for On-line Measurement of Methane

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Semiconductor Photonics and Technology 2004年第2期10卷 141-144页

作者： WANGShu-tao CHERen-sheng LIUJin WANGYu-tian Dept.ofPrecis.Instrum.andMechan. HarbinInstituteofTechnologyHarbin150001CHN CollegeofElectr.Eng. YanshanUniversityQinhuangdao066004CHN

Based on analysis of near infrared spectral absorption of methane,absorption type optical fiber methane gas sensor with high sensitivity using DFB LD as a source is demonstrated. Light source modulation harmonic measurement is presented in this paper. In order to eliminate the noise, the ratio of the fundamental and second-harmonic signals is used. The mathematical model of gas concentration harmonic measurement is built *** detection result of methane concentration is also shown. Experiments have proved a sensitivity of 28×10-6.

关键词： Gas sensor Methane Modulation Harmonic measurement

The Effect of Kaolinite Structure Changes on Its Transformation Temperature of Phases

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Journal of China University of Mining and Technology 2002年第2期12卷 155-157,162页

作者：刘钦甫丁述理张统军李萍 SchoolofResourcesandSafetyEngineering Team115ofShanxiCoalGeologicalExploration HebeiInstituteofArchitecturalScience&Technology

The relationship between kaolinite structure and the temperature of thermal transformation of phase was discussed in this paper through grinding and heating treatment. The results show that the structure of kaolinite is destroyed rapidly with mechanical grinding time, and the kaolinite structure collapses completely after 1 h grinding. The temperature of thermal transformation of phase of decreases with the destruction of kaolinite structure. This result has a great significance for the utilization of kaolinitic rocks in China coal measures.

关键词： kaolinite structure transformation of phase mullite

Progress of Si-based Optoelectronic Devices

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Semiconductor Photonics and Technology 2004年第3期10卷 158-163页

作者： PENGYing-cai FUGuang-sheng WANGYing-long SHANGYong CollegeofElectron.andInform.Eng. HebeiUniversityBaoding071002CHN CollegeofPhys.Sci.andTechnol. HebeiUniversityBaoding071002CHN NaturalScienceFoundationCommitteeofHebeiProvience Shijiazhuang05000CHN

Si-based optoelectronics is becoming a very active research area due to its potential applications to optical *** of the major goals of this study is to realize all-Si optoelectronic integrated *** is due to the fact that Si-based optoelectronic technology can be compatible with Si microelectronic *** Si-based optoelectronic devices and integrated circuits can be achieved,it will lead to a new informational technological *** the article,the current developments of this exciting field are mainly reviewed in the recent *** involved contents are the realization of various Si-based optoelectronic devices,such as light-emitting diodes,optical waveguides devices,Si photonic bandgap crystals,and Si laser,***,the developed tendency of all-Si optoelectronic integrated technology are predicted in the near future.

关键词： Nanocrystalline materials Si-based luminescent devices All-Si optoelectronic integrated technology

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铁诱导低温SiC薄膜的合成(英文)

功能材料与器件学报 2002年第3期8卷 267-270页

作者：于威郑志远刘丽辉傅广生河北大学物理科学与技术学院保定071002

采用热丝化学气相沉积法,以铁作为催化剂,在较低的衬底温度合成纳米SiC薄膜。铁粒子是在400Pa氢气的气氛中,通过用脉冲激光烧蚀铁靶5min引入的。用扫描电镜和拉曼谱对样品进行了分析。扫描电镜观察到了直径为10-30nm,长度短于1μm的无序... 详细信息

采用热丝化学气相沉积法,以铁作为催化剂,在较低的衬底温度合成纳米SiC薄膜。铁粒子是在400Pa氢气的气氛中,通过用脉冲激光烧蚀铁靶5min引入的。用扫描电镜和拉曼谱对样品进行了分析。扫描电镜观察到了直径为10-30nm,长度短于1μm的无序SiC棒。拉曼谱中的横向生子模式的红移表明生长方向的限制效应。所有这些说明Fe粒子的大小将影响到SiC棒的生长。

关键词：铁诱导 HFCVD 催化剂碳化硅薄膜合成

二过碘酸合镍(Ⅳ)配离子氧化氨基乙酸的反应动力学及机理(英文)

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化学研究 2001年第4期12卷 21-25页

作者：单金缓魏海英王莉申世刚孙汉文河北大学化学和环境科学学院保定071002

在碱性介质中于 2 5～ 40℃用分光光度法研究了二过碘酸合镍(Ⅳ)配离子(DPN)氧化氨基乙酸的动力学.结果表明:反应对DPN为准一级,对氨基乙酸为正分数级.准一级速率常数(kobs)随[OH-]增加而增加:随[IO4 -]增加而减小,无盐效应并未检出自... 详细信息

在碱性介质中于 2 5～ 40℃用分光光度法研究了二过碘酸合镍(Ⅳ)配离子(DPN)氧化氨基乙酸的动力学.结果表明:反应对DPN为准一级,对氨基乙酸为正分数级.准一级速率常数(kobs)随[OH-]增加而增加:随[IO4 -]增加而减小,无盐效应并未检出自由基存在,我们假设了一过碘酸合镍(Ⅳ)配离子(MPN)是氧化剂的活性物种,提出包括DPN和MPN存在前期平衡的氧化反应机理。

关键词：二过碘酸合镍(Ⅳ) 配离子氨基乙酸氧化还原反应动力学分光光度法

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中国三麦区小麦抗叶锈基因部署研究

植物病理学报 1996年第1期26卷 19-23页

作者：刘颖超郭爱国朱之 王焕如王剑雄河北农业大学中国农科院植保所

利用已知抗叶锈基因的小麦近等基因系（或单基因系），推导了来自中国３大麦区（东北春麦区、华北平原冬麦区、江淮半冬麦区）的主要小麦品种，待推广品种（系）和抗源材料中可能含有的抗叶锈基因，分析了３大麦区小麦叶锈菌群体的毒性... 详细信息

利用已知抗叶锈基因的小麦近等基因系（或单基因系），推导了来自中国３大麦区（东北春麦区、华北平原冬麦区、江淮半冬麦区）的主要小麦品种，待推广品种（系）和抗源材料中可能含有的抗叶锈基因，分析了３大麦区小麦叶锈菌群体的毒性基因。研究结果表明，东北春麦区的小麦品种可能含有的抗叶锈基因主要有Ｌｒ１６、Ｌｒ２０、Ｌｒ１７等１１个；华北平原冬麦区的小麦品种，可能含有的抗叶锈基因主要是Ｌｒ２６等７个，江淮麦区的小麦品种可能含有的抗叶锈基因主要是Ｌｒ３Ｂｇ、Ｌｒ１０、Ｌｒ２６等９个。对３大麦区小麦叶锈菌群体的毒性基因分析表明，Ｖ１、Ｖ２ｄ、Ｖ３、Ｖ１０、Ｖ２６、Ｖ３３等１３个基因是３大麦区的优势毒性基因，但个别毒性基因在３大麦区的出现频率仍有较大差别。根据基因对基因概念，在基因部署上建议，东北麦区减少含单个无效抗病基因的品种如９０－０５７４４、９１－１１７９、东农８８－５７９７等的种植面积，适当控制含Ｌｒ２０、１７、１５、３０、３Ｋａ的品种，增加沈免系统及小冰麦系统的种植面积；华北麦区增加含Ｌｒ１４ａ、１４ａｂ、１５的品种的种植面积，如ＣＡ９０６９、ＣＡ８６４６、ＣＡ９０７０、Ｃ８９４和冀８７－５１０８等。

关键词：小麦叶锈菌抗性基因部署

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毛细管电泳分离雷公藤有效成分(英文)

河北大学学报（自然科学版） 2001年第2期21卷 183-187页

作者：宋秀荣杨更亮赵敬湘孙汉文李志伟河北大学化学与环境科学学院

用胶束毛细管电泳法分离和测定雷公藤中雷公藤内酯醇和雷公藤内酯酮的含量 .在优化分离条件下 ,2种有效成分的线性范围分别为 0 .0 5349～ 4 .33g/L和 0 .0 186 2～ 3.4 6g/L ;最低检测限分别为0 .0 5349g/L和 0 .0 186 2 g/L .得到满... 详细信息

用胶束毛细管电泳法分离和测定雷公藤中雷公藤内酯醇和雷公藤内酯酮的含量 .在优化分离条件下 ,2种有效成分的线性范围分别为 0 .0 5349～ 4 .33g/L和 0 .0 186 2～ 3.4 6g/L ;最低检测限分别为0 .0 5349g/L和 0 .0 186 2 g/L .得到满意的分离效果 .

关键词：毛细管电泳雷公藤内酯醇雷公藤内酯酮有效成分分离含量测定中药材

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五维3-李代数的分类(英文)

河北大学学报（自然科学版） 2000年第2期20卷 173-176页

作者：李华君张知学保定师范专科学校数学系河北保定071051 河北大学数学系河北保定071002

给出了复数域上2个5维3 -李代数的同构定理 ,通过计算对5维3 -李代数在同构意义下进行了分类 ,给出了基向量的乘法表。

关键词： n-李代数理想幂零性五维分类可解性