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Proinflammatory effects and molecular mechanisms of interleukin-17 in intestinal epithelial cell line HT-29

World Journal of Gastroenterology 2014年第47期20卷 17924-17931页

作者： Yi-Lin Wang Meng Fang Xiao-Ming Wang Wei-Yan Liu Yun-Jiang Zheng Xu-Bo Wu Ran Tao Center for Organ Transplantation and Department of Surgery Ruijin HospitalShanghai Jiaotong University School of Medicine Department of General Surgery Minhang HospitalFudan University Department of Anesthesia The 88th Hospital of PLA Department of Hepatobiliary Surgery Yijishan HospitalWannan Medical College Department of Emergency Xin Hua HospitalShanghai Jiaotong University School of Medicine

AIM:To evaluate the proinflammatory effects and molecular mechanisms of interleukin(IL)-17 in intestinal epithelial cell line ***:HT-29 cells were cultured with IL-17,tumor necrosis factor(TNF)-α,or the combination of both IL-17 and TNF-α.Real-time PCR and Western blot were used to measure the gene expression levels of neutrophil chemokines CXCL1,CXCL2,CXCL5,CXCL6,IL-8and TH-17 cell chemokine CCL20,the phosphorylation levels of p38 and TNF-α,and the expression level of IL-8,after using the p38 inhibitor in HT-29 *** stable Act1 knockdown HT-29 cell line was established to further test the phosphorylation changes of p38,after using IL-17 and TNF-α.RESULTS:After HT-29 cells were cultured with IL-17and TNF-α,the expression levels of neutrophil chemokines(CXCL1,CXCL2,CXCL5,CXCL6,IL-8)and Th17chemokine(CCL20)significantly improved(24.96±2.53,28.47±2.87,38.08±2.72,33.47±2.41,31.7±2.38,44.37±2.73,respectively),and the differences were all statistically significant(P<0.01).Western blot results showed that IL-17 obviously enhanced the phosphorylation level of p38,which was induced by TNF-α.Compared with the control group,the expression level of IL-8 significantly declined(9.47±1.36 vs 3.06±0.67,P<0.01)when TH-29 cells were cultured with IL-17and TNF-α.p38 inhibition assay showed that the p38pathway played an essential role in the inflammatory response induced by IL-17.p38 phosphorylation levels could not be changed after using IL-17 and TNF-αin the stable Act1 knockdown HT-29 cell ***:IL-17 significantly promoted the gene expression levels of TNF-α-induced neutrophil chemokines and Th17 cell *** is obvious that IL-17and TNF-αhave synergistic effects on p38.

关键词： IL-17 HT-29 TNF-α Inflammatory bowel disease

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MicroRNAs and long non-coding RNAs in liver surgery:Diagnostic and therapeutic merits

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Hepatobiliary & Pancreatic Diseases International 2020年第3期19卷 218-228页

作者： Zhi-Qiu Hu Yi Lu Di Cui Chen-Yang Ma Su Shao Ping Chen Ran Tao Jian-Jun Wang Department of Surgery Minhang HospitalFudan UniversityShanghai 201199China Department of Hepatobiliary-Pancreatic&Minimally Invasive Surgery Affiliated Zhejiang Provincial People’s HospitalHangzhou Medical SchoolHangzhou 310014China Center for Clinical Medical Research Affiliated Zhejiang Provincial People’s HospitalHangzhou Medical SchoolHangzhou 310014China Department of General Surgery Chun’an 1st People’s HospitalHangzhou 311700China Department of Obstetrics and Gynecology Shaoxing 2nd HospitalShaoxing 312000China

Background:Hepatectomy and liver transplantation(LT)are the two most commonly performed surgical procedures for various hepatic *** RNA(mi RNA)and long non-coding RNA(lnc RNA)have been gradually unveiled their roles as either biomarkers for early diagnosis or potentially therapeutic tools to manipulate gene expression in many disease *** review aimed to discuss the effects of mi RNA or lnc RNA in the hepatectomy and LT *** sources:We did a literature search from 1990 through January 2018 to summarize the currently available evidence with respect to the effects of mi RNA and lnc RNA in liver regeneration after partial hepatectomy,as well as their involvement in several key issues related to LT,including ischemia-reperfusion injury,allograft rejection,tolerance,recurrence of original hepatic malignancies,***:Certain mi RNAs and lnc RNAs are actively involved in the regulation of various aspects of liver resection and *** the process of liver regeneration after hepatectomy,the expression of mi RNAs and lnc RNAs shows dynamic ***:It is now clear that mi RNAs and lnc RNAs orchestrate in various aspects of the pathophysiological process of LT and *** understanding of the underlying mechanism and future clinical trials may strengthen their positions as either biomarkers or potential therapeutic targets in the management of complications after liver surgery.

关键词： Hepatectomy Liver transplantation microRNA Long non-coding RNA Ischemia-reperfusion injury Rejection

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