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Genetic mapping and expressivity of a wheat multi-pistil gene in mutant 12TP

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Journal of Integrative Agriculture 2019年第3期18卷 532-538页

作者： ZHU Xin-xin NI Yong-jing HE Rui-shi JIANG Yu-mei LI Qiao-yun NIU Ji-shan national Centre of Engineering and Technological Research for Wheat/Key Laboratory of Physiological Ecology and Genetic Improvement of Food Crops in Henan Province/College of Agronomy Henan Agricultural University Shangqiu Academy of agricultural and Forestry sciences

We identified a wheat(Triticum aestivum L.) multi-pistil mutant from an F_2 breeding population in 2012, named 12 TP(three pistils in one floret). Genetic analysis showed that one dominant gene locus controlled the multi-pistil trait. Using homozygous normal and multi-pistil lines(near-isogenic lines; NILs) derived from the original mutant 12 TP, a simple sequence repeat(SSR) marker assay located the 12 TP locus on chromosome arm 2 DL. Four SSR markers were linked to 12 TP and their order was Xcfd233→Xcfd62-12 TP→Xwmc41→Xcfd168 at 15.85, 10.47, 2.89, and 10.37 cM, respectively. The average genetic expressivity of the trait ‘three pistils in one floret' was more than 98% in seven homozygous 12 TP lines; however, the average genetic expressivity in heterozygous F_1 plants was about 49%. Thus, the 12 TP is a semi-dominant gene locus, which differ from all previously reported multi-pistil mutants. Mutant 12 TP is a new useful germplasm for study of wheat floral development and for breeding of high yield wheat.

关键词： wheat multi-pistil mutant expressivity mapping

Enhancement of CH_(3)CO^(*) adsorption by editing d-orbital states of Pd to boost C–C bond cleavage of ethanol eletrooxidation

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science China Chemistry 2024年第2期67卷 696-704页

作者： Yuchen Qin Fengqi Wang Pei Liu Jinyu Ye Qian Wang Yao Wang Guangce Jiang Lijie Liu Pengfang Zhang Xiaobiao Liu Xin Zheng Yunlai Ren Junjun Li Zhicheng Zhang College of sciences Henan Agricultural UniversityZhengzhou450000China Tianjin Key Laboratory of Molecular Optoelectronic sciences Department of ChemistrySchool of ScienceTianjin University&Collaborative Innovation Center of Chemical Science and EngineeringTianjin300072China College of Chemistry and Chemical Engineering Xiamen UniversityXiamen361005China Department of Analysis Sinopec Engineering Group Luoyang R&D Center of TechnologyLuoyang471000China School of Chemical and Material Engineering Jiangnan UniversityWuxi214211China Shandong Provincial Key Laboratory of Chemical Energy Storage and Novel Cell Technology Liaocheng UniversityLiaocheng252000China

Improving the complete ethanol electrooxidation on Pd-based catalysts in alkaline media has drawn widely attention due to the high mass energy ***,the weak adsorption energy of CH_(3)CO^(*) on Pd restricts the C–C bond *** by the molecular orbital theory,we proposed the d-state-editing strategy to construct more unoccupied d-states of Pd for the enhanced interaction with CH_(3)CO^(*) to break C–C *** expected,the reduced number of e_g electrons and more unoccupied d-states of Pd successfully formed on as-prepared porous Rh Au–Pd Cu nanosheets(PNSs).Theoretical calculations show that the optimized d-states of Rh Au–Pd Cu PNS can effectively improve the adsorption of CH_(3)CO^(*) and drastically reduce the energy barrier of C–C bond cleavage,thus boosting the complete oxidation of *** charge ratio of C_1 pathway on Rh Au–Pd Cu PNSs is 51.5%,more than 2 times higher than that of Pd *** finding provides an innovative perspective for the design of highly-efficient noble-based electrocatalysts.

关键词： C–C bond cleavage CH_(3)CO^(*)adsorption unoccupied d-states ethanol oxidation reaction RhAu–PdCu porous nanosheets

Controlled-Release of Plant Volatiles:New Composite Materials of Porous Carbon-Citral and Their Fungicidal Activity against Exobasidium vexans

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Journal of Renewable Materials 2023年第2期11卷 811-823页

作者： Yaoguo Liu Yao Chen Huifang Liu Wei Chen Zhiwei Lei Chiyu Ma Jie Yin Wen Yang College of Tea science Guizhou UniversityGuiyang550025China Guizhou Tea Research Institute Guizhou Academy of Agricultural SciencesGuiyang550006China

Citral(Eo)exhibits excellent fungicidal ***,it is difficult to maintain long-term fungicidal activity due to its strong ***,a controlled-release strategy by using biomass-derived porous carbon(BC)was developed to overcome the drawback of *** composite materials were prepared by loading Eo on tea stem porous carbon(BC@Eo),and their controlled-release fungicidal activity against Exobasidium vexans was *** with a large specific surface area of 1001.6 m2/g and mesoporous structure was fabricated through carbonization tempera-ture of 700℃.The BC@Eo materials were characterized using Fourier-transform infrared spectroscopy and X-ray powder *** results suggested that chemical and physical interactions occurred in BC@*** Eo release profile suggested a biphasic pattern with an initial fast release on days 1–14 and a subsequent controlled phase on days 14–*** in vitro cumulative release percentage of Eo from BC@Eo was 51%during one month,and this result was significantly lower than that from free Eo(cumulative release percentage of Eo of 82%in one week).The anti-fungal activities of Eo and BC@Eo against *** were determined using the inhibition zone *** results indicated that Eo and BC@Eo formed large inhibition zones of 19.66±0.79 and 21.92±0.77 mm,*** influence on the hyphal structure of *** was observed by scanning electron microscopy on day *** hyphal structure of *** treated with BC@Eo was more shrunken than that treated with Eo at 30 days,suggesting that BC@Eo prolongs the fungicidal activity against *** study demonstrated that the encapsulation of Eo in BC for developing the BC@Eo materials could be a promising strategy to inhibit volatility and maintain the fungicidal activity of Eo and provide a potential alternative for the reuse of abundant tea biomass waste resources.

关键词： Exobasidium vexans porous carbon citral controlled release fungicidal activity

TWISTED PRODUCTS AND SMASH PRODUCTS OVER WEAK HOPF ALGEBRAS

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Acta Mathematica Scientia 2004年第2期24卷 247-258页

作者：张良云陈惠香李金其 DepartmentofMathematics NanjingAgriculturalUniversityNanjing210095China

This paper gives a sufficient and necessary condition for twisted products to be weak Hopf algebras, moreover, gives a description for smash products to be weak Hopf algebras. It respectively generalizes ***'s major r... 详细信息

关键词： Weak bialgebra weak Hopf algebra twisted product smash product

Non-Oxidative Methane Conversion Using Lead- and Iron-Modified Albite Catalysts in Fixed-Bed Reactor

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Chinese Journal of Chemistry 2018年第6期36卷 531-537页

作者： Ye Chen Xin Wang Xuegang Luo Xiaoyan Lin Yu Zhang Department of Materials science and Engineering Southwest University of Science and Technology Mianyang Sichuan 621010 China Engineering Research Center of Biomass Materials Ministry of Education Mianyang Sichuan 621010 China

Raw and modified albite catalysts, including Pb/Atbite and Fe/Albite catalysts, have been investigated for methane conversion to C2 hydrocarbons under non-oxidative conditions. Introduction of Pb to albite improved the activity and selectivity to non-coke products. Based on characterization, it was found that Pb entered into the alkali and alkaline-earth metal sites of albite, while partial Fedoped in the tetrahedron sites and the other loaded on the surface of albite. At the reaction temperature of 1073 K, methane gas hourly space velocity （GHSV） of 2 ***-1·h-1, catalyst dosage of 0.25 g （300 mesh）, the methane conversion catalyzed by raw albite in the fixed-bed micro reactor exhibited a methane conversion of 3.32%. Notably, introducing a Pb content of 3.4 wt% into albite greatly enhanced the conversion of methane up to 8.19%, and the selectivity of C2 hydrocarbons reached 99% without any coke under the same reaction conditions. While Fe-doping could weakly heighten the methane conversion to 3.97%, and coke was formed. Thus, a comparison of Pb/Albite and Fe/Albite catalysts demonstrates that the catalytic activity of albite is mainly decided by alkali and alkaline-earth metal sites, and lead-modification can effectively improve the catalytic activity of albite.

关键词： methane conversion albite C2 hydrocarbons active site lead-modification

Multiple generation effects of high temperature on the development and fecundity of Bemisia tabaci （Gennadius）（Hemiptera Aleyrodidae） biotype B

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Insect science 2013年第4期20卷 541-549页

作者： Jian-Ying Guo Lin Cong Fang-Hao Wan State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant Protection Chinese Academy of AgriculturalSciences Beijing China

Insects are ectotherms and their ability to resist temperature stress is limited. The immediate effects of sub-lethal heat stress on insects are well documented, but longer- term effects of such stresses are rarely reported. In this study, survival, development and reproduction of the whitefly, Bemisia tabaci （Gennadius）（Hemiptera： Aleyrodidae） biotype B, were compared over five consecutive generations at 27, 31 and 35℃ and for one generation at 37 ℃. Both temperature and generation significantly affected the fitness of the whitefly. These impacts were more dramatic with increasing generations and temperatures. Among the experimental temperatures, the most favorable for development and reproduction were 27 ℃ and 31 ℃. At 27 ℃, survival, development and fecundity were all stable over these five generations. At 31 ℃, immature survival rate was the highest in the fifth generation, but female fecundities decreased in the fourth and fifth generations. At 35 ℃, egg hatching rate, immature survival rate and female fecundity decreased significantly in the fourth and fifth generations. At 37 ℃, survival ofB. tabaci was not adversely affected, but female fecundity at 37 ℃ was less than 10% of that at 27 ℃ or 31 ℃. These results demonstrate that the lethal high temperature for B. tabaci is over 37 ℃, and the whitefly population continued expanding in the five generations at 35 ℃. The ability orB. tabaci biotype B to survive high temperature stress will play an important role in its population extension under global warming.

关键词： Bemisia tabaci biotype B high temperature stress multiple generation ef-fects population expansion

Rapid generation of genetic diversity by multiplex CRISPR/Cas9 genome editing in rice

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science China(Life sciences) 2017年第5期60卷 506-515页

作者： Lan Shen Yufeng Hua Yaping Fu Jian Li Qing Liu Xiaozhen Jiao Gaowei Xin Junjie Wang Xingchun Wang Changjie Yan Kejian Wang State Key Laboratory of Rice Biology China National Rice Research Institute Chinese Academy of Agricultural Sciences Hangzhou 310006 China Key Laboratory of Plant Functional Genomics Ministry of Education Yangzhou University Yangzhou 225009 China Lianyungang Academy of agricultural sciences Lianyungang 222006 China College of Life sciences Shanxi Agricultural University Taigu 030801 China

The clustered regularly interspaced short palindromic repeats(CRISPR)-associated endonuclease 9(CRISPR/Cas9) system has emerged as a promising technology for specific genome editing in many species. Here we constructed one vector targeting eight agronomic genes in rice using the CRISPR/Cas9 multiplex genome editing system. By subsequent genetic transformation and DNA sequencing, we found that the eight target genes have high mutation efficiencies in the T_0 generation. Both heterozygous and homozygous mutations of all editing genes were obtained in T_0 plants. In addition, homozygous sextuple, septuple, and octuple mutants were identified. As the abundant genotypes in T_0 transgenic plants, various phenotypes related to the editing genes were observed. The findings demonstrate the potential of the CRISPR/Cas9 system for rapid introduction of genetic diversity during crop breeding.

关键词： CRISPR/Cas9 genome editing agronomic genes rice

Evolutionary genomics of climatic adaptation and resilience to climate change in alfalfa

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Molecular Plant 2024年第6期17卷 867-883页

作者： Fan Zhang Ruicai Long Zhiyao Ma Hua Xiao Xiaodong Xu Zhongjie Liu Chunxue Wei Yiwen Wang Yanling Peng Xuanwen Yang Xiaoya Shi Shuo Cao Mingna Li Ming Xu Fei He Xueqian Jiang Tiejun Zhang Zhen Wang Xianran Li Long-Xi Yu Junmei Kang Zhiwu Zhang Yongfeng Zhou Qingchuan Yang Institute of Animal science Chinese Academy of Agricultural SciencesBeijing 100193China national Key Laboratory of Tropical Crop Breeding Shenzhen BranchGuangdong Laboratory of Lingnan Modern AgricultureKey Laboratory of Synthetic BiologyMinistry of Agriculture and Rural AffairsAgricultural Genomics Institute at ShenzhenChinese Academy of Agricultural SciencesShenzhen 518000China School of Grassland science Beijing Forestry UniversityBeijing 100083China Department of Agronomy and Horticulture University of Nebraska-LincolnLincolnNE 68583USA Department of Crop and Soil sciences Washington State UniversityPullmanWA 99163USA U.S.Department of Agriculture-agricultural Research Service Plant Germplasm Introduction and Testing ResearchProsserWA 99350USA national Key Laboratory of Tropical Crop Breeding Tropical Crops Genetic Resources InstituteChinese Academy of Tropical Agricultural SciencesHaikou 571101China

Given the escalating impact of climate change on agriculture and food security,gaining insights into the evolutionary dynamics of climatic adaptation and uncovering climate-adapted variation can empower the breeding of climate-resilient crops to face future climate ***(Medicago sativa ***),the queen of forages,shows remarkable adaptability across diverse global environments,making it an excellent model for investigating species responses to climate *** this study,we performed population genomic analyses using genome resequencing data from 702 accessions of 24 Medicago species to unravel alfalfa’s climatic adaptation and genetic susceptibility to future climate *** found that interspecific genetic exchange has contributed to the gene pool of alfalfa,particularly enriching defense and stress-response *** introgression between *** ***(***)and alfalfa not only aids alfalfa’s climatic adaptation but also introduces genetic burden.A total of 1671 genes were associated with climatic adaptation,and 5.7%of them were introgressions from *** integrating climate-associated variants and climate data,we identified populations that are vulnerable to future climate change,particularly in higher latitudes of the Northern *** findings serve as a clarion call for targeted conservation initiatives and breeding *** also identified preadaptive populations that demonstrate heightened resilience to climate fluctuations,illuminating a pathway for future breeding ***,this study enhances our understanding about the local adaptation mechanisms of alfalfa and facilitates the breeding of climate-resilient alfalfa cultivars,contributing to effective agricultural strategies for facing future climate change.

关键词： Medicago local adaptation population genetics adaptive introgression genetic vulnerability alfalfa breeding

SNP-based identification of QTL for resistance to black point caused by Bipolaris sorokiniana in bread wheat

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The Crop Journal 2022年第3期10卷 767-774页

作者： Qiaoyun Li Runyu Hu Zhenfeng Guo Siyu Wang Chuang Gao Yumei Jiang Jianwei Tang Guihong Yin national Engineering Research Centre for Wheat College of AgronomyNational Key Laboratory of Wheat and Maize Crop ScienceHenan Agricultural UniversityZhengzhou 450002HenanChina

Black point disease caused by Bipolaris sorokiniana is a problem in wheat production *** aimed to identify major quantitative trait loci(QTL)for resistance to black point and develop molecular markers for marker-assisted selection(MAS).A recombinant inbred line(RIL)population derived from a cross between Wanyuanbai 1(susceptible)and SN4143(resistant)was evaluated for black point response at three locations during two years under artificial inoculation with ***,providing data for six *** resistant and 30 susceptible RILs were selected to form resistant and susceptible bulks,respectively,that were genotyped by the wheat 660 K SNP array;685 singlenucleotide polymorphisms(SNPs)were identified,among which 385(56.2%)and 115(16.8%)were located on chromosomes 4 A and 2 B,*** segregant RNA-Seq analysis identified candidate regions on chromosomes 4 A(4.60–40.28 Mb)and 5 A(1.22–48.47 Mb).Genetic linkage maps were constructed for chromosomes 2 B,4 A,and 5 A using 59 polymorphic d CAPS and SSR ***,two QTL,designated ***-4 A and ***-5 A,were detected on chromosomes 4 A and 5 A,*** resistance allele of ***-4 A was derived from SN4143,and that of ***-5 A came from Wanyuanbai ***-4 A with a large and consistent effect(15.1%)is likely to be a new locus for black point *** markers linked to ***-4 A and ***-5 A have potential application in MASbased breeding.

关键词： Black point Molecular marker Quantitative trait locus Triticum aestivum