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Cyclooxygenase-2 expression is dependent upon epidermal growth factor receptor expression or activation in androgen independent prostate cancer

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Asian Journal of Andrology 2008年第5期10卷 758-764页

作者： Rui-Peng Jia Lu-Wei Xu Qi Su Jian-Hua Zhao Wen-Cheng Li Feng Wang Zheng Xu Department of Urology Nanjing First Hospital Affiliated to Nanjing Medical University Nanjing 210006 China Department of Pathology Nanjing First Hospital Affiliated to NanjingMedical University Nanjing 210006 China Center laboratory Nanjing First Hospital Affiliated to Nanjing Medical University Nanjing 210006 China

Aim： To investigate the expression of cyclooxygenase-2 （COX-2） and epidermal growth factor receptor （EGFR） and the possible mechanism in the development in androgen independent prostate cancer （AIPC）. Methods： Immunohistochemistry was performed on paraffin-embedded sections with goat polyclonal against COX-2 and mouse monoclonal antibody against EGFR in 30 AIPC and 18 androgen dependent prostate cancer （ADPC） specimens. The effect of epidermal growth factor （EGF） treatments on the expression of COX-2 and signal pathway in PC-3 and DU-145 cells was studied using reverse transcription-polymerase chain reaction （RT-PCR） and Western blot analysis. ELISA was used to measure prostaglandin E2 （PGE2） levels in the media of PC-3 and DU-145 incubated with EGF for 24 h. Results： COX-2 was positively expressed in AIPC and ADPC, which were predominantly in endochylema of prostate cancer （PCa） cells. Intense staining was seen in AIPC （80%） and in ADPC （55.5%）, but there was no significant association between the two groups. EGFR expression was also positive in the two groups （61.8% in ADPC and 90% in AIPC, P 〈 0.01）. A significant association was found between EGFR expression and a higher Gleason score （P 〈 0.05） or tumor stage （P 〈 0.05）. The expression of PGE2 was increased in PC-3 and DU-145 cells after being incubated with EGF. Both p38MAPK and PI-3K pathway were involved in the PC-3 cell COX-2 upregulation course. In DU- 145, only p38MAPK pathway was associated with COX-2 upregulation. Conclusion： EGFR activation induces COX-2 expression through PI-3K and/or p38MAPK pathways. COX-2 and EGFR inhibitors might have a cooperative anti-tumor effect in PCa.

关键词： cyclooxygenase-2 epidermal growth factor receptor prostatic neoplasms

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Genetic Analysis of the Resistance to Aspergillus flavus Infection in Maize (Zea mays L.)

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Agricultural Sciences in China 2009年第6期8卷 761-765页

作者： DENG De-xiang JIANG Si-xia WANG Yi-jun BIAN Yun-long CHEN Jian-jian JIA Bo jiangsu province key laboratory of Crop Genetics and Physiology~key laboratory of Plant functional genomics Ministry of Education Yangzhou University Yangzhou 225009 P.R. China Yangzhou Environment and Resources Technical College Yangzhou 225009 P.R. China

Maize （Zea mays L.）, one of main crops in the world, is easily susceptible to Aspergillusflavus （Link： fr） infection, resulting huge loses worldwide. Breeding for A. flavus resistance has been proved an efficient way to solve the problem of aflatoxin contamination. Genetic analysis of the sources of resistance to *** in maize is necessary for this purpose. The complete diallel crosses of 6 inbred lines with different resistance to *** infection were implemented. Inoculation categorical data of each cross were analyzed with the additive-dominant and additive-dominant-epitasis genetic models. Results indicated some crosses fitted the 2 major genes with additive-dominant-epitasis genetic model. Others fitted the major gene and polygene mixed model. Moreover, the additive, dominant, and epitasis effects varied in crosses. The *** resistance was controlled by both major gene and polygene.

关键词： maize Aspergillus flavus resistance genetic model

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Morphological Structure and Genetic Mapping of New Leaf-Color Mutant Gene in Rice (Oryza sativa)

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Rice science 2012年第2期19卷 79-85页

作者： LI Yu-hong WANG Bao-he DAI Zheng-yuan LI Ai-hong LIu Guang-qing Zuo Shi-min ZHANG Hong-xi PAN Xue-biao key laboratory of Crop Genetics and Physiology of jiangsu province Yangzhou University Yangzhou 225009 China Lixiahe Region Agricultural Research Institute of jiangsu province Nanjing Subcenter of National Rice Improvement Center Yangzhou 225007 China

Leaf-color mutations are a widely-observed class of mutations, playing an important role in the study of chlorophyll biosynthesis and plant chloroplast structure, function, genetics and development. A naturally-occurring leaf-color rice mutant, Baihuaidao 7, was analyzed. Mutant plants typically exhibited a green-white-green leaf-color progression, but this phenotype was only expressed in the presence of a stress signal induced by mechanical scarification such as transplantation. Prior to the appearance of white ~eaves, mutant plant growth, leaf color, chlorophyll content, and chloroplast ultrastructure appeared to be identical to those of the wild type. After the changeover to white leaf color, an examination of the mutated leaves revealed a decrease in total chlorophyll, chlorophyll a, chlorophyll b, and carotenoid content, a reduction in the number of chloroplast grana lamella and grana, and a gradual degradation of the thylakoid lamellas. At maturity, the mutant plant was etiolated and dwarfed compared with wild-type plants. Genetic analysis indicated that the leaf mutant character is controlled by a recessive nuclear gene. Genetic mapping of the mutant gene was performed using an F2 population derived from a Baihuaidao 7 ~ Jiangxi 1587 cross. The mutant gene was mapped to rice chromosome 11, positioned between InDel markers L59.2-7 and L64.8-11, which are separated by approximately 740.5 kb. The mutant gene is believed to be a new leaf-color mutant gene in rice, and is tentatively designated as gwgl.

关键词： Oryza sativa leaf-color mutant morphological structure genetic analysis gene mapping

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Strategy for Use of Rice Blast Resistance Genes in Rice Molecular Breeding

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Rice science 2020年第4期27卷 263-277页

作者： XIAO Ning WU Yunyu LI Aihong Institute of Agricultural Sciences for Lixiahe Region in jiangsu Yangzhou 225007China jiangsu Collaborative Innovation Center for Modern Crop Production Nanjing 210095China jiangsu key laboratory of Crop genomics and Molecular Breeding Yangzhou UniversityYangzhou 225009China State key laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant ProtectionChinese Academy of Agricultural SciencesBeijing 100193China

Rice blast is one of the most destructive diseases affecting rice production *** development and rational use of resistant varieties has been the most effective and economical measure to control *** this review,we summarized the cloning and utilization of rice blast resistance genes,such as Pi1,Pi2,Pi9,Pi54,Pigm and *** concluded that three main problems in the current breeding of rice blast resistance are:availability of few R(resistance)genes that confer resistance to both seedling and panicle blast,the resistance effect of pyramided lines is not the result of a simple accumulation of resistance spectrum,and only a few R genes have been successfully used for molecular ***,novel utilization strategies for rice blast R genes in molecular breeding were proposed,such as accurately understanding the utilization of R genes in main modern rice varieties,creating a core resistant germplasm with excellent comprehensive traits,screening and utilizing broadspectrum and durable resistance gene ***,the trends and possible development direction of blast resistance improvement were also discussed,including new genes regulating resistance identified via GWAS(genome-wide association study)and improving rice blast resistance using genetic editing.

关键词： rice blast resistance gene molecular breeding strategy

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Cloning and Identification of A New Na^+/H^+ Antiporter Gene ZmSOS1 in Maize(Zea mays L.)

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Agricultural Science & Technology 2009年第6期10卷 57-62,158页

作者：赵祥强南通大学生命科学学院江苏南通226007

[ Objective] The study aimed to clone and identify Na^＋/H^＋ antiporter genes in maize, and provided the information for characterizing the function of such genes in abiotic stress tolerance of maize. Method The in silico cloning, RT-PCR, and bioinformatics analysis were used in this study. Result By in sifico cloning, a plasma membrane Na^＋/H^＋ antiporter gene, named as ZmSOS1 （EMBL accession No. BN001309）, was cloned from maize （ Zea mays L. ）. ZmSOS1 has an open reading frame （ORF） of 3 411 bp which encoded a protein of 1 136 amino acids. By multiple sequence alignment analysis, it showed the predicated peptide of ZmSOS1 were 61% and 82% identities in amino acids to the plasma membrane Na^＋/H^＋ antiporter AtSOS1 and OsSOS1, respectively. The RT-PCR analysis revealed that ZmSOS1 could be significantly up-regulated by salt stress, which indicated ZmSOS1 might play a role in salt tolerance of maize. Conclusion ZmSOS1 is a putative plasma membrane Na^＋/H^＋ antiporter gene and may play a role in abiotic stress tolerance of maize.

关键词： Zea mays Na^+/H^+ antiporter In silico cloning Bioinformatics analysis

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