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How Do Phytochromes Transmit the Light Quality Information to the Circadian Clock in Arabidopsis？

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Molecular Plant 2014年第11期7卷 1701-1704页

作者： Miii Yeom Hyunmin Kim Junhyun Lim Ah- Young Shin Sunghyun Hong Jeong-II Kim Hong Gil Nam Division of Molecular and Life sciences PohangUniversity of Science and Technology Hyojadong Pohang Gyeongbuk 790-784 Republic of Korea Integrative Biosciences & Biotechnology in POSTECH Hyojadong Pohang Gyeongbuk 790-784 Republic of Korea Department of Molecular Biotechnology and Kumho Life science Laboratory Chonnam National University Gwangju 500-757 Republic of Korea Center for Plant Aging Research Institute for Basic Science Daegu 711-873 Republic of Korea Department of New Biology DGIST Hyeongpoong-Myun Dalsung-gun Daegu 711-873 Republic of Korea

Dear Editor, Plants use light as an environmental signal to coor- dinate diverse physiological and developmental processes, thereby increasing their fitness. Light quality, quantity, and photoperiod change periodically under natural condi- tions of daily and seasonal cycles. Plants have developed a circadian clock to respond to these predictable, periodic environmental changes, providing plants with the ability to anticipate daily and seasonal environmental changes.

关键词： STABILITY PROTEIN COP1

Facile synthesis of fully ordered L10-FePt nanoparticles with controlled Pt-shell thicknesses for electrocatalysis

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Nano Research 2017年第8期10卷 2866-2880页

作者： Yonghoon Hong Hee Jin Kim Daehee Yang Gaehang Lee Ki Min Nam Myung-Hwa Jung Young-Min Kim Sang-II Choi Won Seok Seo Department of Chemistry Sogang University Seou104107 Republic of Korea Department of Chemistry and Green-Nano Materials Research Center Kyungpook National University Daegu 41566 Repubfic of Korea Korea basic science institute and University of science and Technology Daejeon 34133 Republic of Korea Department of Chemistry Mokpo National University Jeonnam 58554 Republic of Korea Department of Physics Sogang University Seoul 04107 Republic of Korea Center for Integrated Nanostructure Physics Institute for Basic Science (IBS) Suwon 16419 Repubfic of Korea Department of Energy science Sungkyunkwan University (SKKU) Suwon 16419 Republic of Korea

We report a simple one-step approach for the synthesis of -4 nm uniform and fully L10-ordered face-centered tetragonal （fct） FePt nanoparticles （NPs） embedded in -60 nm MCM-41 （fct-FePt NPs@MCM-41）. We controlled the Pt-shell thickness of the fct-FePt NPs by treating the fct-FePt NPs@MCM-41 with acetic acid （HOAc） or hydrochloric acid （HC1） under sonicafion, thereby etching the surface Fe atoms of the NPs. The fct-FePt NPs deposited onto the carbon support （fct-FePt NP/C） were prepared by mixing the fct-FePt NPs@MCM-41 with carbon and subsequently removing the MCM-41 using NaOH. We also developed a facile method to synthesize acid-treated fct-FePt NP/C by using a HF solution for simultaneous surface-Fe etching and MCM-41 removal. We studied the effects of both surface-Fe etching and Pt-shell thickness on the electrocatalytic properties of fct-FePt NPs for the methanol oxidation reaction （MOR）. Compared with the non-treated fct-FePt NP/C catalyst, the HOAc-treated and HCl-treated catalysts exhibit up to 34% larger electrochemically active surface areas （ECASAs）; in addition, the HCl-treated fct-FePt NP （with -1.0 nm Pt shell）/C catalyst exhibits the highest specific activity. The HF-treated fct-FePt NP/C exhibits an ECASA almost 2 times larger than those of the other acid-treated fct-FePt NP/C catalysts and shows the highest mass activity （1,435 mA·mgPt^-1, 2.3 times higher than that of the commercial Pt/C catalyst） and stability among the catalysts tested. Our findings demonstrate that the surface-Fe etching for the generation of the Pt shell on fct-FePt NPs and the Pt-shell thickness can be factors for optimizing the electrocatalysis of the MOR.

关键词： FePt face centered tetragonal bimetallic methanol oxidation reaction electrocatalysts

相位分布管理的准二维钙钛矿薄膜在纳秒脉冲激光泵浦下实现高效放大自发发射

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science China Materials 2024年第3期67卷 921-930页

作者：韦晓燕刘征征杨洁唐孝生王艳平黄业雄姚亿洪徐新鑫皮明雨杜鹃张丁可 School of Physics and Electronic Engineering Chongqing Normal UniversityChongqing 401331China State Key Laboratory of High Field Laser Physics and CAS Center for Excellence in Ultra-intense Laser science Shanghai Institute of Optics and Fine MechanicsChinese Academy of SciencesShanghai 201800China College of Optoelectronic Engineering Chongqing University of Posts and TelecommunicationsChongqing 400065China Chongqing Research institute Changchun University of Science and TechnologyChongqing 401135China School of Physics and Optoelectronic Engineering Hangzhou Institute for Advanced StudyUniversity of Chinese Academy of SciencesHangzhou 310024China

准二维钙钛矿表现出独特的光物理性质,这使它们成为激光技术潜在进步的优秀候选材料.高性能纳秒脉冲激光和放大自发发射(ASE)是实现连续波抽运光电泵浦激光器的关键工艺.然而,在纳秒脉冲激光泵浦下发展准二维钙钛矿ASE或激光仍然存在一... 详细信息

准二维钙钛矿表现出独特的光物理性质,这使它们成为激光技术潜在进步的优秀候选材料.高性能纳秒脉冲激光和放大自发发射(ASE)是实现连续波抽运光电泵浦激光器的关键工艺.然而,在纳秒脉冲激光泵浦下发展准二维钙钛矿ASE或激光仍然存在一些困难,包括表面缺陷浓度高、俄歇复合和能量传递效率低.本文通过无添加剂的Cs^(+)阳离子掺杂策略,可以很好地控制准二维钙钛矿膜的形貌和相分布,从而解决了低n相导致的能量传递途径不足的问题.本文通过快速的能量传递过程揭示了增益性质和载流子复合.基于此,掺杂适当比例Cs^(+)阳离子的PEA_(2)(FA_(0.7)Cs_(0.3))_(2)Pb_(3)Br_(10)钙钛矿膜的净增益系数比原始钙钛矿薄膜提高了近3倍,从而将纳秒激光激发下的放大自发发射阈值降低到1/3.研究结果表明,在准二维钙钛矿中掺杂Cs^(+)可为实现高性能激光器件的相位分布设计提供见解.

关键词： quasi-2D perovskite cesium cation amplified spontaneous emission energy transfer phase distribution

A simple, flexible and high-throughput cloning system for plant genome editing via CRISPR-Cas system

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Journal of Integrative Plant Biology 2016年第8期58卷 705-712页

作者： Hyeran Kim Sang-Tae Kim Jahee Ryu Min Kyung Choi Jiyeon Kweon Beum-Chang Kang Hyo-Min Ahn Suji Bae Jungeun Kim Jin-Soo Kim Sang-Gyu Kim Center for Genome Engineering Institute for Basic Science Department of Chemistry Seoul National University

CRISPR-Cas9 system is now widely used to edit a target genome in animals and plants. Cas9 protein derived from Streptococcus pyogenes（Sp Cas9） cleaves double-stranded DNA targeted by a chimeric single-guide RNA（sg RNA）. For plant genome editing, Agrobacterium-mediated T-DNA transformation has been broadly used to express Cas9 proteins and sg RNAs under the control of Ca MV 35 S and U6/U3 promoter, respectively. We here developed a simple and high-throughput binary vector system to clone a 19 20 bp of sg RNA, which binds to the reverse complement of a target locus, in a large T-DNA binary vector containing an Sp Cas9 expressing cassette. Twostep cloning procedures：（1） annealing two target-specific oligonucleotides with overhangs specific to the Aar I restriction enzyme site of the binary vector; and（2） ligating the annealed oligonucleotides into the two Aar I sites of the vector, facilitate the high-throughput production of the positive clones. In addition, Cas9-coding sequence and U6/U3 promoter can be easily exchanged via the GatewayTMsystem and unique Eco RI/Xho I sites on the vector, respectively. We examined the mutation ratio and patterns when we transformed these constructs into Arabidopsis thaliana and a wild tobacco, Nicotiana attenuata. Our vector system will be useful to generate targeted large-scale knock-out lines of model as well as non-model plant.

关键词： Aar I-mediated sg RNA cloning CRISPR-Cas9 T-DNA binary vector Exchangeable U6/U3 promoter Gateway compatible Cas9 cloning

K2Ti6O13 Nanoparticle-Loaded Porous rGO Crumples for Supercapacitors

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Nano-Micro Letters 2020年第1期12卷 115-123页

作者： Chongmin Lee Sun Kyung Kim Hankwon Chang Hee Dong Jang Department of Nanomaterials science and Engineering University of Science and TechnologyYuseong‑guDaejeon 34113Republic of Korea Resources Utilization Research Center Korea Institute of Geoscience and Mineral ResourcesYuseong‑guDaejeon 34132Republic of Korea

One-dimensional alkali metal titanates containing potassium,sodium,and lithium are of great concern owing to their high ion mobility and high specific surface *** those titanates are combined with conductive materials such as graphene,carbon nanotube,and carbon nanofiber,they are able to be employed as efficient electrode materials for *** hexa-titanate(K2Ti6O13,KTO),in particular,has shown superior electrochemical properties compared to other alkali metal titanates because of their large lattice parameters induced by the large radius of potassium ***,we present porous rGO crumples(PGC)decorated with KTO nanoparticles(NPs)for application to *** KTO NP/PGC composites were synthesized by aerosol spray pyrolysis and post-heat *** NPs less than 10 nm in diameter were loaded onto PGCs ranging from 3 to 5μ*** porous structure of the composites was obtained by the activation of rGO by adding an excessive amount of KOH to the *** KTO NP/PGC composite electrodes fabricated at the GO/KOH/TiO2 ratio of 1:3:0.25 showed the highest performance(275 F g−1)in capacitance with different KOH concentrations and cycling stability(83%)after 2000 cycles at a current density of 1 A g−1.

关键词： Potassium hexa-titanate Porous graphene crumples Supercapacitors

Highly efficient gene silencing and bioimaging based on fluorescent carbon dots in vitro and in vivo

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Nano Research 2017年第2期10卷 503-519页

作者： Seongchan Kim Yuri Choi Ginam Park Cheolhee Won Young-Joon Park Younghoon Lee Byeong-Su Kim DaI-Hee Min Center for RNA Research Institute for Basic Sciences (IBS) Department of Chemistry Seoul National University Seou108826 Republic of Korea Department of Energy Engineering and Department of Chemistry Ulsan National Institute of Science and Technology (UNIST) Ulsan 44919 Republic of Korea institute of Nanobio Convergence Technology Lemonex Inc. Seou108826 Republic of Korea College of Pharmacy Ajou University Worldcuplo 206 Yeongtong-gu Suwon 16499 Republic of Korea Department of Chemistry Korea Advanced Institute of Science and Technology (KAIST) Daejeon 34141 Republic of Korea

Small interfering RNA （siRNA） is an attractive therapeutic candidate for sequence- specific gene silencing to treat incurable diseases using small molecule drugs. However, its efficient intracellular delivery has remained a challenge. Here, we have developed a highly biocompatible fluorescent carbon dot （CD）, and demonstrate a functional siRNA delivery system that induces efficient gene knockdown in vitro and in vivo. We found that CD nanoparticles （NPs） enhance the cellular uptake of siRNA, via endocytosis in tumor cells, with low cytotoxicity and unexpected immune responses. Real-time study of fluorescence imaging in live cells shows that CD NPs favorably localize in cytoplasm and successfully release siRNA within 12 h. Moreover, we demonstrate that CD NP-mediated siRNA delivery significantly silences green fluorescence protein （GFP） expression and inhibits tumor growth in a breast cancer cell xenograft mouse model of tumor-specific therapy. We have developed a multifunctional siRNA delivery vehicle enabling simultaneous bioimaging and efficient downregulation of gene expression, that shows excellent potential for gene therapy.

关键词： bioimaging carbon dot gene delivery RNA interference targeted cancer therapy

Protective Effect of Brassica napus *** against Inflammation Response in RAW 264.7 Cells

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Chinese Journal of Integrative Medicine 2021年第4期27卷 273-279页

作者： Su-Hyeon Cho Song Rae Kim Myeong Seon Jeong Miri Choi SeonJu Park Kil-Nam Kim Chuncheon Center Korea Basic Science InstituteChuncheon(24341)Republic of Korea

Objective:To demonstrate the anti-inflammatory activity of Brassica napus ***(BNH)in lipopolysaccharide(LPS)-stimulated RAW 264.7 ***:Composition analysis of BNH was conducted via gas chromatography-mass spectrometry after BNH were *** nitric oxide(NO)production was measured using the Griess *** E2(PGE2)production was evaluated with enzyme-linked immunosorbent *** effects of BNH on LPS-induced pro-inflammatory enzymes including inducible nitric oxide synthase(iNOS)and cyclooxygenase-2(COX-2)were evaluated using Western blot ***,phosphorylation of nuclear factor-kappa B(N F-k B)and nuclear translocation of N F-k B p65 were evaluated with Western blot analysis and immunofluorescence staining,***:Compared with LPS-stimulated cells,BNH markedly decreased the generation of NO and PGE_(2)in LPS-stimulated RAW 264.7 cells(P<0.01 or P<0.05).Moreover,BNH inhibited protein levels of iNOS and COX-2(P<0.01).Phosphorylation of NF-k B and nuclear translocation of NF-k B p65 was significantly inhibited by BNH(P<0.01 or P<0.05).Conclusion:The anti-inflammatory activities of BNH were mediated via blockage of the N F-k B signaling pathways in LPS-stimulated RAW 264.7 cells.

关键词： Brassica napus L. inflammation lipopolysaccharide RAW 264.7 cells hydrosols

Installing a molecular truss beam stabilizes MOF structures

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npj Computational Materials 2022年第1期8卷 1110-1118页

作者： Hong Ki Kim Jong-Yeong Jung Gyumin Kang Mu-Hyun Baik Eun-Young Choi Department of Chemistry Korea Advanced Institute of Science and Technology(KAIST)Daejeon34141Republic of Korea Center for Catalytic Hydrocarbon Functionalizations Institute for Basic Science(IBS)Daejeon34141Republic of Korea Korea science Academy of KAIST 105-47 Baegyanggwanmun-roBusanjin-guBusan47162Republic of Korea

Enhancing the stability and durability of metal-organic frameworks(MOFs)is vital for practical applications because many promising MOF materials suffer from phase transitions and/or structural decompositions with humidity being a particularly damaging *** mechanical engineering,the frame of buildings and furniture can be stabilized significantly by installing a truss *** the same principle,we functionalized the organic component of MOF-5 to contain a carbazole moiety that can act as a molecular truss beam by reaching across the corner and forming a stableπ–πinteraction with a phenyl group on the edge position of the *** structural support enhanced the stability of the MOF substantially,allowing the designed MOF to maintain compositional integrity under steam conditions at 90℃for~5 *** unmodified MOF-5 shows clear signs of structural collapse after~1 h.

关键词： beam moiety durability

Biocompatible custom ceria nanoparticles against reactive oxygen species resolve acute inflammatory reaction after intracerebral hemorrhage

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Nano Research 2017年第8期10卷 2743-2760页

作者： Dong-Wan Kang Chi Kyung Kim Han-Gil Jeong Min Soh Taeho Kim In-Young Choi SeuI-Ki Ki Do Yeon Kim Wookjin Yang Taeghwan Hyeon Seung-Hoon Lee Laboratory of Innovative Nanobiotechnology Biomedical Research Institute Seoul National University Hospital Seoul 03080 Republic of Korea Department of Neurology Seoul National University Hospital Seou103080 Republic of Korea Department of Neurology Korea University Guro Hospital and Korea University College of Medicine Seou108308 Republic of Korea Center for Nanoparticle Research Institute for Basic Science (IBS) Seoul 151-742 Republic of Korea School of Chemical and Biological Engineering and institute of Chemical Processes Seoul National University Seoul 151-742 Republic of Korea

Intracerebral hemorrhage （ICH） is a devastating subtype of stroke with a high mortality rate, for which there currently is no effective treatment. A perihematomal edema caused by an intense inflammatory reaction is more deleterious than the hematoma itself and can result in neurological deterioration and death. Ceria nanoparticles （CeNPs） are potent free radical scavengers with potential for biomedical applications. As oxidative stress plays a major role in post-ICH inflammation, we hypothesized that CeNPs might protect against ICH. To test this hypothesis, core CeNPs were synthesized using a modified reverse micelle method and covered with phospholipid-polyethylene glycol （PEG） to achieve biocompatibility. We investigated whether our custom-made biocompatible CeNPs have protective effects against ICH. The CeNPs reduced oxidative stress, hemin-induced cytotoxicity, and inflammation in vitro. In a rodent ICH model, intravenously administered CeNPs were mainly distributed in the hemorrhagic hemisphere, suggesting that they could diffuse through the damaged blood-brain barrier. Moreover, CeNPs attenuated microglia/macrophage recruitment around the hemorrhagic lesion and inflammatory protein expression. Finally, CeNP treatment reduced the brain edema by 68.4% as compared to the control. These results reveal the great potential of CeNPs as a novel therapeutic agent for patients with ICH.

关键词： ceria nanoparticles intracerebral hemorrhage free radical injury anti-inflammation neuroprotective agents biomedical application