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海峡两岸三地环境与资源学术研讨会暨第二届中国环境资源与生态保育学会会员代表大会

作者： LIANG Renjun~(1,2),QI Xiangzhen~2 1.School of Chemistry & Resources Environment,linyi Normal University,P.R.China,276005 2.Jiangsu Key Laboratory of Environmental Change and Ecological Construction,P.R.China,210046

By using analysis on ecological footprints through comparison of ecological footprint and ecological capacity,in this paper,the principle of ecological footprint is elaborated and also the ecological footprint of linyi in 2006 is practiced and *** show that per capita ecological footprint is 1.9737 hm,per capita ecological capacity is 0.3564 hm,per capita ecological deficit is 1.6173 *** ecological footprint need of ten thousands yuan GDP is 0.6958 hm,being greatly lower than the national average *** the economic and social developments were in an unsustainable development ***, the reasons for the high ecological deficit in linyi were analyzed and the countermeasures of how to lower the ecological deficit and realize sustainable development were put forward.

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Bone Marrow Angiogenesis in Patients Presenting with Differential Chinese Medicine Syndrome:Correlation with the Clinico-pathological Features of Aplastic Anemia

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Chinese Journal of Integrative Medicine 2013年第12期19卷 905-912页

作者：吴迪炯叶宝东胡致平沈一平沈建平林圣云陈明涛刘永林周郁鸿 Department of Hematology the First Affiliated HospitalZhejiang University of Traditional Chinese Medicine

Objective： To explore differences in bone marrow angiogenesis seen in aplastic anemia （AA） patients presenting with differential Chinese medicine （CM） syndrome, and to correlate these differences with clinical pathology. Methods： Thirty-five patients were enrolled, including 18 with ＂yang deficiency syndrome＂ and 17 with ＂yin deficiency syndrome.＂ Bone marrow biopsies and serum were collected. Microvessel density （MVD） and positive expression of vascular endothelial-derived growth factor （VEGF） were detected by immunohistochemisty. Hypoxia inducible factor -α （HIF-α ）, and VEGF expression were assayed by enzyme-linked immunoabsorbent assay （ELISA）, serum lactate dehydrogenase （LDH） was tested by enzyme method and liquid chip technology was used to detected the expression of interleukin （IL）-2, IL-4, IL-6, IL-10, interferon （IFN）-＇y and tumor necrosis factor （TNF）-α. Results： Counts for leukocytes, absolute neutrophils and platelets in ＂yin deficiency syndrome＂ were lower than those found in ＂yang deficiency syndrome＂（P〈0.05）. MVD and VEGF expression, and the positive rate of CD34 and VEGF in bone marrow were lower in hA, especially in ＂yin deficiency syndrome＂（P〈0.01 or P〈0.05）. ＂Yin deficiency syndrome＂ displayed decreased VEGF and LDH expression, and enhanced expression of HIF-α as compared to ＂yang deficiency syndrome＂（P〈0.05）. Levels of IL-4 and IL-6 were higher in AA （P〈0.01）, but IL-10 was decreased （P〈0.05）. High TNF-c~ expression was seen in ＂yang deficiency syndrome＂ and IFN- γ expression was decreased in ＂yin deficiency syndrome＂ as compared with normals （P〈0.01 and P〈0.05, respectively）. Conclusion： AA patients have lower MVD than normals, especially in ＂yin deficiency syndrome.＂ MVD might differentially correlate to disease severity, and could be dependent on bone marrow or serum VEGF expression and LDH. Additionally, IL-2, IL-10, IL-4 and IFN- γ were negatively associated while IL-6 and TNF- α were positively associated with MVD.

关键词： aplastic anemia angiogenesis Chinese medicine syndrome clinico-pathological vascular endothelial- derived growth factor tumor necrosis factor-αL

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Effect of kidney-reinforcing,blood-activating and stasis-removing recipes on adhesion molecule expression of bone marrow mesenchymal stem cells from chronic aplastic anemia patients

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Journal of Traditional Chinese Medicine 2012年第4期32卷 596-603页

作者： Baodong Ye Dan Chen Dijiong Wu Xiangping Wu Xiang Zhang Yuhong Zhou Yiping Shen Keding Shao Qinhong Yu Department of Hematology The First Affiliated HospitalZhejiang University of Traditional Chinese Medicine

OBJECTIVE： To explore the effect of kidney-rein- forcing, blood-activating and stasis-removing recipes on adhesion molecule expression of bone mar- row mesenchymal stem cells （MSCs） from patients with chronic aplastic anemia （CAA）. METHODS： We used three Traditional Chinese Medicine recipes, namely a kidney-reinforcing recipe （KRR）, blood-activating and stasis-removing recipe （BASRR）, and kidney-reinforcing, blood-activating and stasis-removing recipe （KRBASFIR）, and a nor- mal saline control to prepare herbal medicine se- rum in Sprague Dawley rats. Thirty CAA patients were enrolled in the experimental group, including 17 kidney-Yang deficient patients and 13 kidney-Yin deficient patients. Ten healthy individuals were included in the control group. MSCs were isolated from bone marrow samples, and the cell density was observed to measure their proliferation ability by microscopy on days 2, 7, and 14 after isolation. In addition, the expression of adhesion molecules of bone marrow MSCs （CD106, CD49d, CD31 and CD44） were detected by flow cytometry after 48 h of treatment with the four different herbal medi- cine serums. RESULTS： The proliferation of MSCs from kid- ney-Yang deficient and kidney-Yin deficient pa- tients was weaker than that of MSCs from the con- trol group. The expression of all adhesion mole- cules of bone marrow MSCs from CAA patients was obviously lower than that in the control group （P〈 0.01）. The expression of CD49d and CD31 in MSCs from patients with a kidney-Yin deficiency was low- er than in those with a kidney-yang deficiency （P〈 0.05 and P〈O.01, respectively）. For kidney-Yang defi- cient patients, CD31 expression in the KRBASRR group was significantly higher than that in the BASRR group （P〈O.01）, while CD44 in the KRBASRR group was significantly higher than that in both KRR and BASRR groups （P〈O.01）. For kidney-Yin defi- cient patients, CD106 and CD49d expression in the KRBASRR group was obviously higher than that in the KRR group （P〈0.05）, wh

关键词： Anemia Aplastic Bone marrow Mesen-chymal stem cells Cell adhesion molecules Rein-forcing kidney Blood-activating stasis-removing

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Analysis of the Coupling between Ecosystems and Economy in Mountainous Regions—A Case Study in Anji County of Zhejiang Province in China

Analysis of the Coupling between Ecosystems and Economy in M...

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海峡两岸三地环境与资源学术研讨会暨第二届中国环境资源与生态保育学会会员代表大会

作者： ZHANG Yunqi~(1,2) 1 School of Chemistry and Resources Environment,linyi Normal University,linyi 276000,China 2 Research Center for Eco-Environmental sciences,CAS,Beijing,100085,China

This paper aims to analyse the coupling between ecosystem and economy(CBEE) in mountainous regions in a spatial *** research is carried out in Anji County of Zhejiang *** sea level to the peak,according to altitude variation of every 100 m,the whole county is divided into 16 space *** of Anji County are composed of 9 types of land *** GDP and the CBEE of every type of land covers on every space unit is calculated with statistical data and results of field surveys. Subsequently,the ratio of the CBEE to the VES and the ratio of the CBEE to the GDP of every type of land cover on every space unit are worked out *** indicate that,with altitude descending,more and more VES is exploited and economy's output *** the whole county,14.34%of local VES,97% of which is the value of material productions,has been exploited into local GDP,meanwhile the VES contributes 12.12%of the GDP.

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Study on Agrobacterium tumefaciens-mediated Transformation of Brassica campestris L. with Fusion Gene Ycoil-bFGF

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Agricultural science & technology 2009年第4期10卷 31-36页

作者：徐岩肖艳双杜金霞汪洪郑伟李营庞实锋生物反应器与药物开发教育部工程研究中心吉林长春130118 吉林农业大学生命科学学院吉林长春130118 吉林农业大学中药材学院吉林长春130118 温州医学院药学院浙江温州325035

[ Objective] The study is to generate pharmaceutical protein via plant transgenic technique. [Methed] Using the cotyledons with petiole as transformation receptor, the fusion gene of rapeseed oil-body gene and bFGF was introduced into the rapeseed （ Brassica campestris L. ） by Agrobacterium tumefaciens-mediated transformation; meanwhile regeneration conditions of rapeseed were also optimized, and the regenerated resistant plantlets were detected by PCR and Southern blot. [ Result] This fusion gene had been integrated into rapeseed genome successfully, and the optimized conditions of transformation and regeneration were as follows： explants pre-culture for 2 d, co-culture for 3 d, bacteria solution OD600 for 0.3 and infection time for 5 min. [ Conclusion] The results laid a solid foundation for extraction, isolation and purification of protein in transgenic plant seeds.

关键词： Basic fibroblast growth factor (bFGF) Plant bioreactor Oil-body system Agrobacterium tumefaciens Brassica campestris L.

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