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A telomere-to-telomere gap-free reference genome of watermelon and its mutation library provide important resources for gene discovery and breeding

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Molecular Plant 2022年第8期15卷 1268-1284页

作者： Yun Deng Shoucheng Liu Yilin Zhang Jingsheng Tan Xiaopeng Li Xiao Chu Binghua Xu Yao Tian Yudong Sun Bosheng Li YunbiXu Xing Wang Deng Hang He Xingping Zhang Peking University Institute of Advanced Agricultural Sciences Shandong Laboratory of Advanced Agricultural Sciences at WeifangWeifangShandong 261000China School of Advanced Agricultural Sciences and School of Life Sciences State Key Laboratory of Protein and Plant Gene ResearchPeking UniversityBeijing 100871China Jiangsu Xuhuai Area Huaiyin Institute of Agricultural Science HuaianJiangsu 223300China

Watermelon,Citrullus lanatus,is the world's third largest fruit *** genomes with gaps and a narrow genetic base hinder functional genomics and genetic improvement of ***,we report the assembly of a telomere-to-telomere gap-free genome of the elite watermelon inbred line G42 by incorporating high-coverage and accurate long-read sequencing data with multiple assembly *** 11 chromosomes have been assembled into single-contig pseudomolecules without gaps,representing the highest completeness and assembly quality to *** G42 reference genome is 369321829 bp in length and contains 24205 predicted protein-coding genes,with all 22 telomeres and 11 centromeres ***,we established a pollen-EMS mutagenesis protocol and obtained over 200000M1 seeds from *** a sampling pool,48 monogenic phenotypic mutations,selected from 223M1and 78 M2 mutants with morphological changes,were *** average mutation density was 1 SNP/1.69Mband1 indel/4.55 Mb per M1 plant and 1SNP/1.08Mb and 1 indel/6.25 Mb per M2 *** advantage of the gap-free G42 genome,8039 mutations from 32 plants sampled from M1 and M2 families were identified with 100%accuracy,whereas only 25% of the randomly selected mutations identified using the 97103v2 reference genome could be *** this library and the gap-free genome,two genes responsible for elongated fruit shape and male sterility(CiMs1)were identified,both caused by a single basechange from G to *** validated gap-free genome and its EMS mutation library provide invaluable resources for functional genomics and genetic improvement of watermelon.

关键词： watermelon gap-free genome EMS,mutation library gene discovery genome-wide sequencing genic male sterility

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A simple and efficient CRISPR/Cas9 system permits ultra-multiplex genome editing in plants

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The Crop Journal 2024年第2期12卷 569-582页

作者： Suting Wu Htin Kyaw Zhijun Tong Yirong Yang Zhiwei Wang Liying Zhang Lihua Deng Zhiguo Zhang Bingguang Xiao William Paul Quick Tiegang Lu Guoying Xiao Guannan Qin Xue'an Cui Biotechnology Research Institute Chinese Academy of Agricultural SciencesBeijing 100081China Key Laboratory of Genetics Breeding and Multiple Utilization of CropsMinistry of EducationCollege of AgricultureFujian Agriculture and Forestry UniversityFuzhou 350002FujianChina Key Laboratory of Biological Breeding for Fujian and Taiwan Crops Ministry of Agriculture and Rural AffairsCollege of AgricultureFujian Agriculture and Forestry UniversityFuzhou 350002FujianChina Key Laboratory of Agro-ecological Processes in Subtropical Region Institute of Subtropical AgricultureChinese Academy of SciencesChangsha 410125HunanChina School of Biosciences The University of SheffieldFirth CourtWestern BankSheffield S102TNUK Key Laboratory of Tobacco Biotechnological Breeding National Tobacco Genetic Engineering Research CenterYunnan Academy of Tobacco Agricultural SciencesKunming 650021YunnanChina

The development and maturation of the CRISPR/Cas genome editing system provides a valuable tool for plant functional genomics and genetic *** available genome-editing tools have a limited number of targets,restricting their application in genetic *** this study,we developed a novel CRISPR/Cas9 plant ultra-multiplex genome editing system consisting of two template vectors,eight donor vectors,four destination vectors,and one primer-design software *** combining the advantages of Golden Gate cloning to assemble multiple repetitive fragments and Gateway recombination to assemble large fragments and by changing the structure of the amplicons used to assemble sg RNA expression cassettes,the plant ultra-multiplex genome editing system can assemble a single binary vector targeting more than 40 genomic loci.A rice knockout vector containing 49 sg RNA expression cassettes was assembled and a high co-editing efficiency was *** plant ultra-multiplex genome editing system advances synthetic biology and plant genetic engineering.

关键词： CRISPR/Cas9 Multiplex genome editing Assembly system Plant

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Bridge Crack Segmentation Method Based on Parallel Attention Mechanism and Multi-Scale Features Fusion

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Computers, Materials & Continua 2023年第3期74卷 6485-6503页

作者： Jianwei Yuan Xinli Song Huaijian Pu Zhixiong Zheng Ziyang Niu College of Civil Engineering Changsha University of Science and TechnologyChangsha410114China College of Computer&Communication Engineering Changsha University of Science and TechnologyChangsha410114China China Construction Fifth Engineering Bureau Co. Ltd.Changsha410004China

Regular inspection of bridge cracks is crucial to bridge maintenance and *** traditional manual crack detection methods are timeconsuming,dangerous and *** the same time,for the existing mainstream vision-based automatic crack detection algorithms,it is challenging to detect fine cracks and balance the detection accuracy and ***,this paper proposes a new bridge crack segmentationmethod based on parallel attention mechanism and multi-scale features fusion on top of the DeeplabV3+network ***,the improved lightweight MobileNetv2 network and dilated separable convolution are integrated into the original DeeplabV3+network to improve the original backbone network Xception and atrous spatial pyramid pooling(ASPP)module,respectively,dramatically reducing the number of parameters in the network and accelerates the training and prediction speed of the ***,we introduce the parallel attention mechanism into the encoding and decoding *** attention to the crack regions can be enhanced from the aspects of both channel and spatial parts and significantly suppress the interference of various ***,we further improve the detection performance of the model for fine cracks by introducing a multi-scale features fusion *** research results are validated on the self-made *** experiments show that our method is more accurate than other *** intersection of union(IoU)and F1-score(F1)are increased to 77.96%and 87.57%,*** addition,the number of parameters is only 4.10M,which is much smaller than the original network;also,the frames per second(FPS)is increased to 15 frames/*** results prove that the proposed method fits well the requirements of rapid and accurate detection of bridge cracks and is superior to other methods.

关键词： Crack detection DeeplabV3+ parallel attention mechanism feature fusion

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Nb_(2)CT_(x)MXene boosting PEO polymer electrolyte for all-solid-state Li-S batteries:two birds with one stone strategy to enhance Li+conductivity and polysulfide adsorptivity

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Rare Metals 2023年第8期42卷 2562-2576页

作者： Si-Ming Liu Meng-Xun Chen Ying Xie Deng-Hua Liu Jin-Fei Zheng Xiang Xiong Heng Jiang Li-Chang Wang Heng Luo Kai Han State Key Laboratory for Powder Metallurgy College of Chemistry and Chemical EngineeringCentral South UniversityChangsha 410083China Shinghwa Advanced Material Group Co. LtdDongying 257500China School of Physics and Electronics Central South UniversityChangsha 410083China Department of Chemistry Oregon State UniversityCorvallis OR 97331-4003USA School of Geosciences and Info-Physics Central South UniversityChangsha 410083China

All-solid-state lithium-sulfur(Li-S)battery is regarded as next-generation high energy density and safety battery *** key challenge is to develop a compatible high-performance solid-state ***,a two birds with one stone strategy is proposed to simultaneously enhance Li+conductivity and polysulfide adsorptivity of poly(ethylene oxide)(PEO)-based polymer electrolyte via the integration of Nb_(2)CT_(x)***,the sheet size of Nb_(2)CT_(x)MXene is crucial for the enhancement of Li^(+)conductivity and polysulfide adsorptivity,attributing to the difference in a specific surface area related to the percolation *** tuning the sheet size of Nb_(2)CT_(x)MXene from 500-300 nm to below 100 nm,the ionic conductivity of the PEO electrolyte is increased to2.62×10^(-4)S·cm^(-1)with improved Li+transference number of 0.37 at ***,theoretical calculation and X-ray photoelectron spectroscopy(XPS)conjointly prove that poly sulfides could be effectively adsorbed by Nb2CTxnanosheets via forming Nb-S bonding to inhibit their shuttle in the PEO *** a result,the all-solid-state Li-S cell exhibits an initial capacity of 1149 mAh·g^(-1)at 0.5C and good cycling stability with 491 mAh·g^(-1)after 200 *** results demonstrate the necessity of polysulfide inhibition and the application of Nb_(2)CT_(x)MXene in PEO-based electrolytes for all-solid-state Li-S batteries.

关键词： All-solid-state Li-S batteries Polyethylene oxide(PEO)-based electrolyte Nb_(2)CT,MXene Shuttle effect inhibition

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Genetic diversity,population structure and core collection analysis of hunan tea plant germplasm through genotyping-by-sequencing

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Beverage Plant Research 2022年第1期2卷 36-42页

作者： Feiyi Huang Jihua Duan Yu Lei Zhen Liu Yankai Kang Yi Luo Yingyu Chen Youyong Li Shuoqian Liu Saijun Li Zhonghua Liu College of Horticulture Hunan Agricultural UniversityChangsha 410128China Tea Research Institute Hunan Academy of Agricultural Sciences/National Centre for Tea ImprovementHunan Branch/Hunan Tea Variety and Seedling Engineering Technology Research CenterChangsha 410125China Tea Research Institute Yunnan Academy of Agricultural SciencesMenghai 666200China

Tea is considered to be a well-known and widely consumed beverage and hunan province is rich in tea plant *** order to better conserve and utilize hunan tea plant resources,110 tea accessions from seven geographical origins were used to assess genetic diversity of hunan tea plant germplasm through genotyping by sequencing(GBS)*** a result,a total of 311,044 high-quality single nucleotide polymorphism(SNP)markers were *** structure,phylogenetic relationships and principal component analysis(PCA)divided the entire accessions into three *** genetic diversity and population differentiation analysis showed that the mean observed heterozygosity(Ho)ranged from 0.16 to 0.24,while the mean polymorphic information content(PIC)ranged from 0.14 to 0.17,and mean minor allele frequency(MAF)ranged from 0.11 to *** of molecular variance(AMOVA)indicated that 81.38%of the total variance was derived from within populations,which suggested a rich genetic diversity in hunan tea ***,a core tea germplasm set was developed,which was comprised of 22 tea plant accessions and maintained the whole genetic diversity of the entire *** work should be valuable for conservation and utilization of tea germplasm in hunan。

关键词： hunan germplasm analysis

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Enteromorpha polysaccharide-zinc replacing prophylactic antibiotics contributes to improving gut health of weaned piglets

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Animal Nutrition 2021年第3期7卷 641-649页

作者： Chunyan Xie Yumei Zhang Kaimin Niu Xiaoxiao Liang Haihua Wang Junwei Shan Xin Wu College of Resources and Environment Hunan Agricultural UniversityChangshaHunan 410128China Key Laboratory of Agro-ecological Processes in Subtropical Region Institute of Subtropical AgricultureThe Chinese Academy of SciencesNational Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production Hunan Provincial Engineering Research Center for Healthy Livestock and Poultry ProductionChangshaHunan 410125China Institute of Biological Resources Jiangxi Academy of SciencesNanchang 330096China Henan Zhongke Ground Food Co. Ltd.ZhengzhouHenan 450001China Qingdao Seawin BioteSch Group Co. LtdQingdao266071China

This research aimed to study whether Enteromorpha polysaccharide-zinc(EP-Zn)can act as an alternative to antibiotics in weaned piglet *** hundred and twenty-four weaned piglets from 14 pens were randomly assigned into 1 of 2 groups according to their body weight and litter size(7 pens/group).The piglets in the antibiotics group were fed with olaquindox at 400 mg/kg and enduracidin at 800 mg/kg basal diet,and piglets in the EP-Zn group were fed with EP-Zn at 800 mg/kg basal *** piglet per pen was selected to collect samples after 14 d of *** showed that EP-Zn supplementation significantly increased the plasma anti-oxidants level compared with the antibiotics ***,a nonsignificant difference was observed in growth performance between treatment ***,the intestinal tight junction(TJ)protein expression and the histopathologic evaluation data showed that EP-Zn contributed to improving intestinal ***,piglets in the EP-Zn group had a lower level of intestinal inflammation-related cytokines including IL-6(P<0.001),IL-8(P<0.05),IL-12(P<0.05)and tumor necrosis factor-α(TNF-α)(P<0.001),and showed an inhibition of the phosphor-ylation nuclear transcription factor-kappa B(p-NF-kB)(P<0.05)and total NF-kB(P<0.001)level in the jejunal *** together,it is supposed that EP-Zn,to some extent,would be a potent alternative to prophylactic antibiotics in improving the health status of weaned piglets.

关键词： Enteromorpha polysaccharide-zinc chelate Prophylactic antibiotic Intestinal development Weaned piglet

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A Novel Evaluation Strategy to Artificial Neural Network Model Based on Bionics

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Journal of Bionic Engineering 2022年第1期19卷 224-239页

作者： Sen Tian Jin Zhang Xuanyu Shu Lingyu Chen Xin Niu You Wang School of Mathematics and Statistics Hunan Normal UniversityChangsha410081China College of Information Science and Engineering Hunan Normal UniversityChangsha410081China School of Computer and Communication Engineering Changsha University of Science and TechnologyChangsha410114China Key Laboratory of Industrial Control Technology Zhejiang UniversityHangzhou310058China Science and Technology on Parallel and Distributed Laboratory College of ComputerNational University of Defense TechnologyChangsha410199China Key Laboratory of Industrial Control Technology Institute of Cyber Systems and ControlZhejiang UniversityHangzhou310027China

With the continuous deepening of Artificial Neural Network(ANN)research,ANN model structure and function are improving towards diversification and ***,the model is more evaluated from the pros and cons of the problem-solving results and the lack of evaluation from the biomimetic aspect of imitating neural networks is not inclusive ***,a new ANN models evaluation strategy is proposed from the perspective of bionics in response to this problem in the ***,four classical neural network models are illustrated:Back Propagation(BP)network,Deep Belief Network(DBN),LeNet5 network,and olfactory bionic model(KIII model),and the neuron transmission mode and equation,network structure,and weight updating principle of the models are analyzed *** analysis results show that the KIII model comes closer to the actual biological nervous system compared with other models,and the LeNet5 network simulates the nervous system in ***,evaluation indexes of ANN are constructed from the perspective of bionics in this paper:small-world,synchronous,and chaotic ***,the network model is quantitatively analyzed by evaluation indexes from the perspective of *** experimental results show that the DBN network,LeNet5 network,and BP network have synchronous *** the DBN network and LeNet5 network have certain chaotic characteristics,but there is still a certain distance between the three classical neural networks and actual biological neural *** KIII model has certain small-world characteristics in structure,and its network also exhibits synchronization characteristics and chaotic *** with the DBN network,LeNet5 network,and the BP network,the KIII model is closer to the real biological neural network.

关键词： Artificial neural network(ANN) Back Propagation(BP)network Deep Belief Network(DBN) LeNet5 network Olfactory bionic model(KIII model) Small world Chaos Synchronous

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Performance analysis of UAV-based mixed underwater PLC-RF systems

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Digital Communications and Networks 2024年第5期10卷 1332-1340页

作者： Shenghai Chen Liang Yang Xianfu Lei College of Computer Science and Electronic Engineering Hunan University Key Laboratory of Intelligent Control Technology for Wuling-Mountain Ecological Agriculture in hunan Province Huaihua University School of Information Science and Technology Southwest Jiaotong University

In this study,we analyzed the performance of an Unmanned Aerial Vehicle(UAV)-based mixed Underwater Power Line Communication-Radio Frequency(UPLC-RF) *** this network,a buoy located at the sea is used as a relay to transmit signals from the underwater signal source to the UAV through the PLC *** assume that the UPLC channel obeys a log-normal distribution and that the RF link follows the Rician *** this model,we obtained the closed-form expressions for the Outage Probability(OP),Average Bit-error-rate(ABER),and Average Channel Capacity(ACC).In addition,the asymptotic analysis of the OP and ABER was performed,and an upper bound for the average capacity was ***,the analytical results were verified by Monte Carlo simulation thereby demonstrating the effect of impulse noise and the altitude of the UAV on network performance.

关键词： ABER Underwater power line communication Average bit-error-rate ACC Average channel capacity OP Outage probability UAV Unmanned aerial vehicle UPLC

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Effect of Multi-Hydroxyl Polymer-Treated MUF Resin on the Mechanical Properties of Particleboard Manufactured with Reed Straw

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Journal of Renewable Materials 2023年第9期11卷 3417-3431页

作者： Yuhui Huang Zhiyuan Yin Ming Liu Meng Li Yingfeng Zuo Yan Qing Yiqiang Wu College of Materials Science and Engineering Central South University of Forestry and TechnologyChangsha410004China Department of Human Resources Shengao Technology Co.Ltd.Hangzhou311217China

The poor bonding performance between aqueous adhesives represented by melamine-urea formaldehyde(MUF)resins and reed straw hinders their applications in the field of non-wood-based ***-hydroxyl polymers are highly reactive and are often used as *** study fabricated a resin with a strengthened crosslinked structure by combining a multi-hydroxyl polymer and MUF resin *** reed particleboard was prepared by using this resin as an adhesive and reed stalk as the *** results show that neighboring molecules combined to form C–O–C bonds that strengthened the cross-linked structure of the *** addition,the viscosity of the resin was increased,and a continuous adhesive layer on the surface of reed particles was formed,which slowed the penetration of reed *** adhesive layer significantly improved the mechanical properties of the reed *** maximum modulus of rupture(MOR),modulus of elasticity(MOE),and internal bonding strength(IB)of the reed particleboard were 33.53,4126,and 0.79 MPa,*** IB of the board was 3.3 times higher than that of the reed particleboard prepared with a conventional MUF *** straw is a non-wood biomass material that has the advantage of sustainable development and may replace woodbased materials to produce *** resin-prepared reed particleboard is expected to be used in areas such as custom furniture and engineering materials.

关键词： Multi-hydroxyl polymers melamine-urea formaldehyde(MUF)resin cross-linked reed particleboard mechanical property

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Quantitative Trait Locus Analysis for Rice Yield Traits under Two Nitrogen Levels

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Rice science 2015年第3期22卷 108-115页

作者： FENG Yue ZHAI Rong-rong LIN Ze-chuan CAO Li-yong WEI Xing-hua CHENG Shi-hua Chinese National Center for Rice Improvement / State Key Laboratory of Rice Biology China National Rice Research Institute Institute of Crop and Nuclear Technology Utilization Zhejiang Academy of Agricultural Sciences

A recombinant inbred line population derived from a super hybrid rice Xieyou 9308（Xieqingzao B/Zhonghui 9308） and its genetic linkage map were used to detect quantitative trait loci（QTLs） for rice yield traits under the low and normal nitrogen（N） levels. A total of 52 QTLs for yield traits distributed in 27 regions on 9 chromosomes were detected, with each QTL explaining 4.93%–26.73% of the phenotypic variation. Eleven QTLs were simultaneously detected under the two levels, and 30 different QTLs were detected under the two N levels, thereby suggesting that the genetic bases controlling rice growth under the low and normal N levels were different. QTLs for number of panicles per plant, number of spikelets per panicle, number of filled grains per panicle, and grain density per panicle under the two N levels were detected in the RM135–RM168 interval on chromosome 3. QTLs for number of spikelets per panicle and number of filled grains per panicle under the two N levels, as well as number of panicles per plant and grain density per panicle, under the low N level, were detected in the RM5556–RM310 interval on chromosome 8. The above described QTLs shared similar regions with previously reported QTLs for rice N recycling.

关键词： nitrogen quantitative trait locus rice yield trait

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