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Optimization of fermentation factors to enhance rice straw degradation ability using a microbial consortium LZF-12

International Journal of Agricultural and Biological Engineering 2019年第3期12卷 168-173页

作者： Guoxiang Zheng Chenyang Zhou Ting Yin Zhaoxin Lu Shuang Ai College of Engineering Northeast Agricultural UniversityHarbin 150030China Key Laboratory of Pig-breeding Facilities Engineering Ministry of Agriculture and Rural AffairsHarbin 150030China heilongjiang Key Laboratory of Technology and Equipment for the Utilization of Agricultural Renewable Resources Harbin 150030China

Biological pretreatment has broad application prospects in agricultural waste treatment because of its economic benefits,environmental protection and energy saving *** this study,a microbial consortium LZF-12 was applied for a biological pretreatment to degrade rice *** experiments were performed under hydrolysis conditions by using the method of Box-Behnken factorial design(BBD).The results showed that the model multiple correlation coefficient R2 was 0.9816,and the effects of three factors on the degradation of rice straw of LZF-12 as descending order were the initial rice content,chicken manure content,and initial pH *** interaction between straw concentration,chicken manure concentration,and initial pH value had significant effects on the degradation of a microbial *** the optimum conditions of 0.86%rice straw,0.5%chicken manure and the initial pH value of 7.0,the degradation rate of rice straw reached 72.4%.There is only a small difference of 0.55%between the experimental value and predicted value from BBD ***,it is feasible for the established model due to the consistent results between the prediction and experimental *** microbial consortium LZF-12 has high cellulase enzyme activities and degradation ability over a wide range of temperature and pH value,indicating that it has a good development potential and application prospects in waste biodegradation and biomass energy production.

关键词： microbial consortium biological pretreatment biodegradation rice straw optimization

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Stability Analysis of a Lignocellulose Degrading Microbial Consortium

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Journal of Northeast Agricultural University(English Edition) 2018年第1期25卷 55-61页

作者： Zheng Guo-xiang Li Jian Zhou Chen-yang Chong Yu-ting College of Engineering Northeast Agricultural UniversityHarbin 150030China Key Laboratory of Pig-breeding Facilities Engineering Ministry of AgricultureHarbin 150030China heilongjiang Key Laboratory of Technology and Equipment for the Utilization of Agricultural Renewable Resources Harbin 150030China

An active mesophilic lignocellulose degrading microbial consortium, designated LZF-12, was bred from humus-rich soil by successive subcultivation under facultative aerobic static condition. Batch experiments were performed to investigate the structural and functional stability of lignocellulose degradation of rice straw of 10 g · L-1. The results showed that efficient degradation of rice straw(>70%) could be achieved and acetic acid concentration accounted for over 70% of total aqueous products from different generations by microbial consortium LZF-12 within 7 days. Denaturing gradient gel electrophoresis(DGGE) and sequencing of 16 S r DNA sequences amplified from the total consortium DNA representing the presence of sequences were related to those of Clostridium, Clostridium cellulolyticum, Pseudomonas, Acetivibrio and some uncultured bacteria in LZF-12. DGGE pattern profiles from different LZF-12 generations were reproducible, suggesting the relative stabilities of the microbial community structure and succession mechanism in the established consortium.

关键词： microbial consortium rice straw degradation stability

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Effect of electroacupuncture on knee osteoarthritis and relevant inflammatory factors:A randomized clinical trial

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World Journal of Acupuncture-Moxibustion 2022年第4期32卷 329-335页

作者： Yong LIU Hao WU First Department of Acupuncture and Moxibustion First Affiliated Hospital of Heilongjiang University of Chinese MedicineHarbin 150040Heilongjiang ProvinceChina Graduate School of heilongjiang University of Chinese Medicine Harbin 150040Heilongjiang ProvinceChina

Objective:To explore the treatment effects of electroacupuncture(EA),acupuncture with filiform needle,and western medication for knee osteoarthritis(KOA).Methods:It was a randomized,controlled trial with the blinding of outcome assessors and statistician.90 outpatients were diagnosed as KOA in Department of Acupuncture and Moxibustion,the First Affiliated Hospital of heilongjiang University of Traditional Chinese *** the random number table,they were divided into a medication group,an acupuncture group and an EA group,30 cases in each *** the medication group,routine medication was provided with oral administration of celebrex for 21 *** acupuncture was applied in the remaining groups,at Liángqiū(梁丘ST34),Xuèhǎi(血海SP10),Dúbí(犊鼻ST35),Nèixīyǎn(内膝眼EX-LE4),Yánglíngquán(阳陵泉GB34),Hèdǐng(鹤顶EX-LE2)and Sānyīnjiāo(三阴交SP6)and the needles were retained for 30 *** the EA group,electric stimulation with low-frequency pulse current and dense wave was applied for 30 min on the basis of the treatment of the acupuncture *** treatment was applied once daily at 1-day intervals after each 6-day treatment for a total of 21 *** Ontario and McMaster University Osteoarthritis Index(WOMAC)and visual analogy scale(VAS)scores and levels of serum inflammatory factors(interleukin-1β[IL-1β]and tumor necrosis factor[TNF-α])were used to assess the clinical therapeutic ***:Following treatment,there were significant differences in the WOMAC score in the medication,acupuncture,and EA groups after treatment(all P<0.01).In the comparison among groups,after treatment,the WOMAC score in the EA group was lower than that in either the acupuncture or medication group(both P<0.01).Compared to before treatment,VAS scores were significantly different in the medication group(3.95±0.55 vs 5.75±1.40),the acupuncture group(2.78±0.38 vs 5.78±1.44)and EA group(1.72±0.38 vs 5.78±1.39)separately after treatment(all P<0.01).In the comparison among groups,after treatment

关键词： Knee osteoarthritis Pain Acupuncture Electroacupuncture Dense wave

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电针治疗膝骨关节炎以及对相关炎症因子水平的影响(英文)

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World Journal of Acupuncture-Moxibustion 2022年

作者：刘勇吴昊黑龙江中医药大学研究生院黑龙江中医药大学第一附属医院针灸一科

Objective: To explore the treatment effects of electroacupuncture (EA)， acupuncture with filiform needle， and western medication for knee osteoarthritis (KOA).Methods: It was a randomized， controlled trial with the blinding of outcome assessors and statistician. 90 outpatients were diagnosed as KOA in Department of Acupuncture and Moxibustion， the First Affiliated Hospital of heilongjiang University of Traditional Chinese Medicine. Using the random number table， they were divided into a medication group， an acupuncture group and an EA group， 30 cases in each one. In the medication group， routine medication was provided with oral administration of celebrex for 21 days. Regular acupuncture was applied in the remaining groups， at Liángqiū (梁丘ST34)， Xuèh?i (血海SP10)， Dúbí (犊鼻ST35)， Nèixīy?n (内膝眼EX-LE4)， Yánglíngquán (阳陵泉GB34)， Hèd?ng (鹤顶EX-LE2) and Sānyīnjiāo (三阴交SP6) and the needles were retained for 30 min. In the EA group， electric stimulation with low-frequency pulse current and dense wave was applied for 30 min on the basis of the treatment of the acupuncture group. The treatment was applied once daily at 1-day intervals after each 6-day treatment for a total of 21 days. Western Ontario and McMaster University Osteoarthritis Index (WOMAC) and visual analogy scale (VAS) scores and levels of serum inflammatory factors (interleukin-1β [IL-1β] and tumor necrosis factor [TNF-α]) were used to assess the clinical therapeutic ***: Following treatment， there were significant differences in the WOMAC score in the medication， acupuncture， and EA groups after treatment (all P<0.01). In the comparison among groups， after treatment， the WOMAC score in the EA group was lower than that in either the acupuncture or medication group (both P<0.01). Compared to before treatment， VAS scores were significantly different in the medication group (3.95 ± 0.55 vs 5.75 ± 1.40 )， the acupuncture group (2.78 ± 0.38 vs 5.78 ± 1.44) and EA group (1.72 ± 0.38 vs 5.78 ± 1.39) separately

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