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Screening and validation of reference genes for qPCR analysis in gonads and embryos of Takifugu bimaculatus

Aquaculture and Fisheries 2022年第3期7卷 278-286页

作者： Zhaowei Zhong Lulu Ao Liping Zhao Zeyu Zhang Yonghua Jiang Key Laboratory of Healthy Mariculture for East China Sea Ministry of AgricultureFisheries CollegeJimei UniversityXiamen361021China college of Animal Sciences Fujian Agriculture and Forestry UniversityFuzhou350002China college of Food and Biological Engineering Jimei UniversityXiamen361021China

Suitable reference genes are one of the necessary conditions for obtaining reliable results by real-time fluorescence quantitative PCR(qPCR).In this study,the expression of the 10 common candidate reference genes(18s rRNA,rps27,cnbp,rpl7,ube2,hsp-at,gapdh,β-actin,rpl13a,1-ef1a)at different developmental stages of gonad and embryo of Takifugu bimaculatus were analyzed by qPCR.And the expression stability of these reference genes is analyzed by GeNorm,NormFinder and Bestkeeper softwares.The results showed that the expression stability of 1-ef1a was the highest(p<0.05)combined with the results of three analysis softwares,and the optimal number of reference genes was 3,which were 1-ef1a,hsp-at and rps27.Based on this result,we analyzed the expression of objective genes(foxl2 and dmrt1)at different developmental stages(early,medium-term and late)of gonads and embryos(eye vesicle stage,motility stage,heart anlage stage,retinal pigmentation stage,newly hatched larva)of T.bimaculatus by qPCR.Among which the highest expression level of foxl2 was in the middle stage of ovary(p<0.05),and dmrt1 was expressed in the early stage of testis with the highest level(p<0.05).The expression levels of foxl2 and dmrt1 in the early stages of embryonic development were low or even almost non-existent,and had a slight increase in the late embryonic development(newly hatched larva).The expression profile of objective genes are consistent with their roles in the development of gonads and embryos,which indicates that 1-ef1a,hsp-at and rps27 are the suitable reference gene combination for detecting the expression of objective genes by qPCR in gonads and embryos of T.bimaculatus.This research also provides the reliability data for screening the reference genes by qPCR in fish.

关键词： Reference gene screening qPCR Gene expression Takifugu bimaculatu

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Long noncoding RNA H19 regulates degeneration and regeneration of injured peripheral nerves

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中国神经再生研究：英文版 2023年第8期18卷 1847-1851页

作者： Yu-Mei Feng Jian Shao Min Cai Yi-Yue Zhou Yi Yao Jia-Xi Qian Zi-Han Ding Mao-Rong Jiang Deng-Bing Yao School of Life Sciences Key Laboratory of Neuroregeneration of Jiangsu and Ministry of EducationCoinnovation Center of NeuroregenerationNantong UniversityNantongJiangsu ProvinceChina Medical School of Nantong University NantongJiangsu ProvinceChina Nantong University Xinglin college NantongJiangsu ProvinceChina

Our previous studies have shown that long noncoding RNA(lncRNA)H19 is upregulated in injured rat sciatic nerve during the process of Wallerian degeneration,and that it promotes the migration of Schwann cells and slows down the growth of dorsal root ganglion axons.However,the mechanism by which lncRNA H19 regulates neural repair and regeneration after peripheral nerve injury remains unclear.In this study,we established a Sprague-Dawley rat model of sciatic nerve transection injury.We performed in situ hybridization and found that at 4–7 days after sciatic nerve injury,lncRNA H19 was highly expressed.At 14 days before injury,adeno-associated virus was intrathecally injected into the L4–L5 foramina to disrupt or overexpress lncRNA H19.After overexpression of lncRNA H19,the growth of newly formed axons from the sciatic nerve was inhibited,whereas myelination was enhanced.Then,we performed gait analysis and thermal pain analysis to evaluate rat behavior.We found that lncRNA H19 overexpression delayed the recovery of rat behavior function,whereas interfering with lncRNA H19 expression improved functional recovery.Finally,we examined the expression of lncRNA H19 downstream target SEMA6D,and found that after lncRNA H19 overexpression,the SEMA6D protein level was increased.These findings suggest that lncRNA H19 regulates peripheral nerve degeneration and regeneration through activating SEMA6D in injured nerves.This provides a new clue to understand the role of lncRNA H19 in peripheral nerve degeneration and regeneration.

关键词： adeno-associated virus dorsal root ganglion lncRNA H19 nerve degeneration nerve regeneration peripheral nerve rat sciatic nerve injury semaphorin 6D Wallerian degeneration

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Effects of urea-N and CO_(2) coupling fertilization on the growth,photosynthesis,yield and anthocyanin content of hydroponic purple cabbage Brassica campestris ssp.chinensis

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International Journal of Agricultural and Biological Engineering 2023年第5期16卷 123-131页

作者： Danyan Chen Yuanyuan Feng Ya Liu Juan Hu Shilong Li Jingze Ma Xinyu Lu Xiaorui Jiang Shuhui Sun Zhi Yang Changyi Wang college of Horticulture Jinling Institute of TechnologyNanjing 210038China Co-innovation Center for Sustainable Forestry in Southern China College of ForestryNanjing Forestry UniversityNanjing 210037China School of Software Engineering Jinling Institute of TechnologyNanjing 211169China Powerchina Zhongnan Engineering Co. LtdChangsha 410014China college of Agricultural Science and Engineering Hohai UniversityNanjing 210098China China Coal Technology Engineering Group Nanjing Design and Research Institute Co.Ltd Nanjing 210014China

Water and fertilizer coupling is a high-efficiency technology for the development of facility agriculture.However,the interaction effect of nitrogen(N)and air carbon dioxide(CO_(2))on hydroponic purple cabbage,especially on its leaf anthocyanins under hydroponic solution systems,remains unexplored.In this study,six treatments were set as C0N0,C0N2,C0N4,C1N0,C1N2 and C1N4,with N0,N2 and N4 being 0.0 g/L,0.2 g/L and 0.4 g/L exogenous urea-N to hydroponic solution dilution,respectively.C0 and C1 were set as with and without CO_(2)fertilizer(i.e.,800 g CO_(2)agent added one week after transplanting and 600 g CO_(2)agent added when the plant reached 15 cm in height),respectively.Pot experiments were conducted to investigate the interaction effect of N and air CO_(2)(N×CO_(2))on the growth,photosynthesis,yield and anthocyanin content of hydroponic purple cabbage Brassica campestris ssp.chinensis.The results showed N×CO_(2)extremely significantly influenced plant height(H),net photosynthetic rate(Pn),stomatal conductance(Gs),intercellular oxidation concentration(Ci),transpiration rate(Tr),leaf water use efficiency(LWUE)and yield.The C1N0 treatment had the largest yield at 262.5 g/plant,with higher values for root length,root weight,plant height and leaf number than the other treatments.The Pn,Ci and Tr of C1N4 were the highest at 3.05μmol CO_(2)/m2·s,352.8μmol CO_(2)/m2·s and 2.31 mmol H2O/m2·s,respectively.The C1N2 treatment received the largest Gs value of 0.70 mol H2O/m2·s and the largest Tr of 2.31 mmol H2O/m2·s.There was the highest LWUE for C0N2(1.41)and the highest anthocyanin content for C1N2(1.35 mg/kg).There was a significant negative correlation between leaf number and anthocyanin(r=-0.414,p<0.05).The findings demonstrated that adding CO_(2)fertilizer and 0.2 g/L exogenous urea-N to hydroponic solution dilution is a potential N×CO_(2)coupling strategy to increase anthocyanin and the yield of purple cabbage.

关键词： nitrogen concentration carbon dioxide purple cabbage hydroponic solution anthocyanins

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不同方法制备的AgSnO_(2)TiB_(2)电接触材料的微观结构和电弧侵蚀行为

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稀有金属材料与工程 2022年第4期51卷 1164-1171页

作者：刘彦峰王献辉何旋万梦豫苏亚宁叱小彤陈婷婷苗雨王立钊西安理工大学材料科学与工程学院陕西西安710048 商洛学院化学工程与现代材料学院陕西商洛726000

分别采用球磨法、可溶性淀粉模板法和滤纸模板法制备了AgSnO_(2)TiB_(2)复合粉末,并利用火花等离子体烧结技术(SPS)制备了块体材料。对Ag4%SnO_(2)4%TiB_(2)(质量分数)电接触材料的物理性能和电弧侵蚀特性进行了研究。结果表明,模板的... 详细信息

分别采用球磨法、可溶性淀粉模板法和滤纸模板法制备了AgSnO_(2)TiB_(2)复合粉末,并利用火花等离子体烧结技术(SPS)制备了块体材料。对Ag4%SnO_(2)4%TiB_(2)(质量分数)电接触材料的物理性能和电弧侵蚀特性进行了研究。结果表明,模板的空间限域效应有效地改善了增强相在基体中的均匀分散,提高了Ag4%SnO_(2)4%TiB_(2)接触材料的导电率和硬度。与球磨法相比,滤纸模板法和淀粉模板法制备的Ag4%SnO_(2)4%TiB_(2)复合材料的电导率分别增加了12.18倍和9.60倍,显微硬度分别增加了17.10%和33.94%。滤纸模板更有利于SnO_(2)和TiB_(2)的均匀分散,减少集中电弧侵蚀和飞溅损失,因此具有更好的抗弧蚀性。

关键词：模板法电接触材料物理性能电弧侵蚀特性

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dbSCI:A manually curated database of SARS-CoV-2 inhibitors for COVID-19

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BIOCELL 2023年第2期47卷 367-371页

作者： QIANG WANG GUO ZHAO LONGXIANG XIE XUAN LI XIXI YU QIONGSHAN LI BAOPING ZHENG ZULIPINUER WUSIMAN XIANGQIAN GUO Institute of Biomedical Informatics School of SoftwareBioinformatics CenterHenan Provincial Engineering Center for Tumor Molecular MedicineSchool of Basic Medical SciencesAcademy for Advanced Interdisciplinary StudiesHenan UniversityKaifeng475004China

Severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)is the pathogen of the ongoing coronavirus disease 2019(COVID-19)global pandemic.Here,by centralizing published cell-based experiments,clinical trials,and virtual drug screening data from the NCBI PubMed database,we developed a database of SARS-CoV-2 inhibitors for COVID-19,dbSCI,which includes 234 SARS-CoV-2 inhibitors collected from publications based on cell-based experiments,81 drugs of COVID-19 in clinical trials and 1305 potential SARS-CoV-2 inhibitors from bioinformatics analyses.dbSCI provides four major functions:(1)search the drug target or its inhibitor for SARS-CoV-2,(2)browse target/inhibitor information collected from cell experiments,clinical trials,and virtual drug screenings,(3)download,and(4)submit data.Each entry in dbSCI contains 18 types of information,including inhibitor/drug name,targeting protein,mechanism of inhibition,experimental technique,experimental sample type,and reference information.In summary,dbSCI provides a relatively comprehensive,credible repository for inhibitors/drugs against SARS-CoV-2 and their potential targeting mechanisms and it will be valuable for further studies to control COVID-19.

关键词： SARS-CoV-2 COVID-19 Inhibitors Drugs Database

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BmCH25H,a vertebrate interferon-stimulated gene(ISG)homolog,inhibits BmNPV infection dependent on its hydroxylase activity in Bombyx mori

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Insect Science 2023年第2期30卷 321-337页

作者： Hongyun Wu Junming Xia Shigang Fei Yeyuan Wang Mengmeng Zhang Yiyao Guo Xian Li Luc Swevers Jingchen Sun Min Feng Guangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding College of Animal ScienceSouth China Agricultural UniversityGuangzhouChina Insect Molecular Genetics and Biotechnology National Center for Scientific Research DemokritosInstitute of Biosciences and ApplicationsAthensGreece

Cholesterol-25-hydroxylase(CH25H)has been identified as an interferon-stimulated gene(ISG)in mammals that exerts its antiviral effects by catalyzing the conversion of cholesterol to 25-hydroxycholesterol(25HC).However,invertebrates lack an antiviral system homologous to vertebrate interferons(IFNs)because the genomes of invertebrates do not encode IFN-like cytokines.Nevertheless,CH25H is present in insect genomes and it therefore deserves further study of whether and by which mechanism it could exert an antiviral effect in invertebrates.In this study,the Bombyx mori CH25H(BmCH25H)gene,of which the encoded protein has high homology with other lepidopteran species,was identified and located on chromosome 9.Interestingly,we found that the expression of BmCH25H was significantly upregulated in B.mori nucleopolyhedrovirus(BmNPV)-infected BmN cells and silkworm(B.mori)larvae at the early infection stage.The inhibitory effect of BmCH25H on BmNPV replication was further demonstrated to depend on its catalytic residues to convert cholesterol to 25HC.More importantly,we demonstrated that during BmNPV infection,BmCH25H expression was increased through the Janus kinase–signal transducer and activator of transcription(JAK–STAT)pathway,similar to the induction of ISGs following virus infection in vertebrates.This is the first report that CH25H has antiviral effects in insects;the study also elucidates the regulation of its expression and its mechanism of action.

关键词： Bombyx mori nucleopolyhedrovirus Bombyx mori Cholesterol 25-hydroxylase Interferon-stimulated genes Signal transducer and activator of transcription

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Difference in calcium ion precipitation between free and immobilized Halovibrio mesolongii HMY2

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Journal of Environmental Sciences 2022年第12期34卷 184-200页

作者： Huaxiao Yan Meiyu Huang Jihan Wang Heding Geng Xiyu Zhang Ziyang Qiu Yongliang Dai Zuozhen Han Yudong Xu Long Meng Lanmei Zhao Maurice E.Tucker Hui Zhao college of Chemical and Biological Engineering College of Safety and Environmental EngineeringCollege of Earth Science and EngineeringShandong University of Science and TechnologyQingdao 266590China Laboratory for Marine Mineral Resources Center for Isotope Geochemistry and GeochronologyQingdao National Laboratory for Marine Science and TechnologyQingdao 266237China School of Earth Sciences University of BristolBristol BS81RJUK Cabot Institute University of BristolCantock’s CloseBristol BS81UJUK

Biomineralization has become a research focus in wastewater treatment due to its much lower costs compared to traditional methods.However,the low sodium chloride(NaCl)-tolerance of bacteria limits applications to only water with low NaCl concentrations.Here,calcium ions in hypersaline wastewater(10%NaCl)were precipitated by free and immobilized Halovibrio mesolongii HMY2 bacteria and the differences between them were determined.The results show that calcium ions can be transformed into several types of calcium carbonate with a range of morphologies,abundant organic functional groups(C-H,C-O-C,C=O,etc),protein secondary structures(β-sheet,α-helix,3_(10)helix,andβ-turn),P=O and S-H indicated by P2p and S2p,and more negativeδ^(13)C_(PDB)(‰)values(-16.8‰to-18.4‰).The optimal conditions for the immobilized bacteria were determined by doing experiments with six factors and five levels and using response surface method.Under the action of two groups of immobilized bacteria prepared under the optimal conditions,by the 10^(th)day,Ca^(2+)ion precipitation ratios had increased to 79%-89%and 80%-88%with changes in magnesium ion cencentrations.Magnesium ions can significantly inhibit the calcium ion precipitation,and this inhibitory effect can be decreased under the action of immobilized bacteria.Minerals induced by immobilized bacteria always aggregated together,had higher contents of Mg,P,and S,lower stable carbon isotope values and less well-developed protein secondary structures.This study demonstrates an economic and eco-friendly method for recycling calcium ions in hypersaline wastewater,providing an easy step in the process of desalination.

关键词： Biomineralization Halovibrio mesolongii Hypersaline wastewater Calcium ion removal Intracellular aragonite

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Comprehensive Control of Ecological Environment in Water Sources

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Meteorological and Environmental Research 2023年第3期14卷 73-77页

作者： Jiarong ZHANG Shuang WANG Tengfei XU Shaanxi Provincial Key Laboratory of Comprehensive Utilization of Tailings Resources Shangluo UniversityShangluo 726000China

To scientifically and effectively conduct comprehensive control of water sources to achieve water safety,the importance,main steps and plan of comprehensive control of ecological environment in water sources were analyzed on the basis of analyzing the development and protection process of foreign water sources.The results show that for the comprehensive control of water sources,it is needed to pay attention to the accurate grasp of the current environmental quality of water sources and carefully analyze the problems in the ecological environment of water sources.Besides,it is necessary to ensure the water quality and quantity of sources,focus on the implementation of environmental remediation and ecological restoration,implement regional protection of water sources,and combine engineering and non-engineering measures to take comprehensive control of environmental problems.

关键词： Drinking water safety Non-engineering measures Engineering measures Comprehensive control

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Th-MOF showing six-fold imide-sealed pockets for middle-sizeseparation of propane from natural gas

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Nano Research 2023年第2期16卷 3287-3293页

作者： Li Wang Wenhui Zhang Jie Ding Lele Gong Rajamani Krishna Youyuan Ran Lan Chen Feng Luo Jiangxi province Key Laboratory of Synthetic Chemistry School of ChemistryBiology and Material ScienceEast China University of TechnologyNanchang 330013China Van’t Hoff Institute for Molecular Sciences University of AmsterdamScience Park 9041098 XH AmsterdamThe Netherlands

Separation of propane from natural gas is of great importance to industry.However,in light of size-based separation,there still lacks effective method to directly separate propane from natural gas,due to the comparable physical properties for these light alkanes(C1–C4)and the middle size of propane.In this work,we found that a new Th-metal-organic framework(MOF)could be an ideal solution for this issue.The Th-MOF takes UiO-66-type structure,but with the pocket sealed by six-fold imide groups;this not only precisely reduces the size of pocket to exactly match propane,but also enhances the host–guest interactions through multiple(C)H(^(δ+))∙∙∙(^(δ−))O(C)interactions.As a result,highly selective adsorption of propane over methane,ethane,and butane was observed,implying unique middle-size separation.The actual separation was confirmed by breakthrough experiments of simulated natural gas,confirming its superior application in direct separation of propane from natural gas.The separation mechanism,as unveiled by both theoretical calculation and comparative experiments,is due to the six-fold imide-sealed pocket that could effectively distinguish propane from other light alkanes through both size effect and host–guest interactions.

关键词： Th-metal-organic framework(MOF) intermediate-size-separation natural gas selectivity

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Recycling and utilization of coal gasification residues for fabricating Fe/C composites as novel microwave absorbents

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International Journal of Minerals,Metallurgy and Materials 2023年第3期30卷 591-599页

作者： Guomin Li Xiaojie Xue Lutao Mao Yake Wang Lingxiao Li Guizhen Wang Kewei Zhang Rong Zhang Yuexiang Wang Liping Liang School of Materials Science and Engineering Taiyuan University of Science and TechnologyTaiyuan 030024China Key Laboratory of Advanced Materials of Tropical Island Resources of Ministry of Education Hainan UniversityHaikou 570228China State Key Laboratory for Coal Conversion Institute of Coal ChemistryChinese Academy of SciencesTaiyuan 030001China China-Belarus Belt and Road Joint Laboratory on Electromagnetic Environment Effect Taiyuan 030032China

Under the background of a transformation of the global energy structure,coal gasification technology has a wide application prospect,but its by-product,the coal gasification residue(CGR),is still not being efficiently utilized for recycling.The CGR contains abundant carbon components,which could be applied to the microwave absorption field as the carbon matrix.In this study,Fe/CGR composites are fabricated via a two-step method,including the impregnation of Fe^(3+)and the reduction process.The influence of the different loading capacities of the Fe component on the morphology and electromagnetic properties is studied.Moreover,the loading content of Fe and the surface morphology of the Fe/CGR can be reasonably controlled by adjusting the concentration of the ferric nitrate solution.Meanwhile,Fe particles are evenly inserted on the CGR framework,which expands the Fe/CGR interfaces to enhance interfacial polarization,thus further improving the microwave-absorbing(MA)properties of composites.Particularly,as the Fe^(3+)concentration is 1.0 mol/L,the Fe/CGR composite exhibits outstanding performance.The reflection loss reaches-39.3 dB at 2.5 mm,and the absorption bandwidth covers 4.1 GHz at 1.5 mm.In this study,facile processability,resource recycling,appropriately matched impedance,and excellent MA performance are achieved.Finally,the Fe/CGR composites not only enhance the recycling of the CGR but also pioneer a new path for the synthesis of excellent absorbents.

关键词： coal gasification residue recycling utilization composite microwave absorption

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