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Liberica coffee as an alternative cropping system for sustainable farming on Indonesian Peatlands

International Journal of agricultural and Biological Engineering 2024年第1期17卷 180-188页

作者： Bariot Hafif Yulius Ferry Budi Martono Rita Harni Kurnia Dewi Sasmita Busyra B.Saidi Maswar Maswar R.Bambang Heryanto Novizar Nazir Fahmuddin Agus Meynarti Sari Dewi Ibrahim National research and Innovation agency Research Center for Estate CropsIndonesia National research and Innovation agency Research Center for Food CropsIndonesia Faculty of agricultural Technology Andalas UniversityIndonesia

Indonesian peatlands are a fragile ecosystem,and to protect it,growing Liberica coffee is a promising way for both the environment and the economy.This study aimed to evaluate the performance of the liberica coffee on peatland with different water tables and develop an improved cultivation system in this ecosystem.The study area was in Tanjung Jabung Barat District,Jambi Province,Indonesia.The plant age is greater than 15 years and the average plant density was about 1000 trees/hm2.The study was conducted in two stages.The 1st stage was a survey to identify and characterize smallholder liberica coffee farming at three peatland zones,namely 0-100 m,200-300 m,and>400 m from the principal drainage canal,and the 2nd stage was a field experiment to test the effectiveness of amendments in improving liberica coffee growth and improving degraded peatland.The treatments were arranged in a randomized complete block design with four replications,including manure(M),at the rate of 10 t/hm^(2);lime(L),3 t/hm^(2);peat surface elevation(P),and a farmer’s practice(Control)without manure and/or lime as a control.This research revealed that liberica coffee can not tolerate high water table as it may stimulate disease incidence and cause low-quality yield.However,recycling of organic C of about 17.14 t/(hm^(2)·a)through cherry residue application and litter compensated part of the loss of organic C through CO_(2)emissions,coffee bean removal,and maintaining peat fertility for sustainable farming.The low yield of(0.70±0.12)t/(hm^(2)∙a)coffee bean could be increased to(0.87±0.24)and(0.94±0.14)t/(hm^(2)∙a)by adding 3 t/(hm^(2)∙3a)of lime or 10 t/(hm^(2)∙a)of manure,respectively.This research revealed that water table management and amendments are two main factors in liberica coffee farming on peatlands.It is of great significance to study the cultivation technology of coffee in peatland.

关键词： degraded peat amendments water table cultivation system liberica coffee alternative cropping system sustainable farming Indonesian Peatlands

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Co-encapsulation efficiency of silk sericin-alginate-prebiotics and the effectiveness of silk sericin coating layer on the survival of probiotic Lactobacillus casei

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Food Bioscience 2022年第2期46卷 507-516页

作者： Patchanok Apiwattanasiri Ratchanee Charoen Sriwiang Rittisak Kriangkrai Phattayakorn Sakwiboon Jantrasee Wanticha Savedboworn Department of Agro-Industry Technology and Management Faculty of Agro-IndustryKing Mongkut’s University of Technology North BangkokPrachinburiThailand Department of Innovation and Product development Technology Faculty of Agro-IndustryKing Mongkut’s University of Technology North BangkokPrachinburiThailand Department of Food Technology and Nutrition Faculty of Natural Resources and Agro-IndustryKasetsart UniversityChalermphrakiat Sakon Nakhon Province CampusSakon NakhonThailand Department of Chemistry Faculty of ScienceKhon Kaen UniversityMuangKhon KaenThailand

The use of silk sericin protein as a biomaterial to co-encapsulate probiotic Lactobacillus casei TISTR 1463 with alginate and various prebiotics was determined.In addition,0.2%silk sericin was also evaluated as a coating material to improve the viability of probiotic cells.Silk sericin coating has the potential to enhance probiotic survival in freeze-dried microcapsules.Under refrigerated storage condition of 4℃for 120 days,alginate-silk sericin-maltitol with silk sericin coating(ASM-C)provided the best performance with the lowest inactivation rate(k)and the highestδparameter(the first decimal reduction time)applied by the log-linear and Weibull models,respectively.However,the stability was relatively low at the temperature of 30℃.Co-encapsulation of alginate-silk sericin-prebiotic and coated with silk sericin exerted a protective impact on the strain survival when exposed to high temperatures and transited through the digestive system.

关键词： Microencapsulation Silk sericin Probiotic Storage stability

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development of new aromatic rice lines with high eating and cooking qualities

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Journal of Integrative Agriculture 2023年第3期22卷 679-690页

作者： Kanokwan KAEWMUNGKUN Keasinee TONGMARK Sriprapai CHAKHONKAEN Numphet SANGARWUT Thiwawan WASINANON Natjaree PANYAWUT Khanittha DITTHAB Kannika SIKAEWTUNG QI Yong-bin Sukanya DAPHA Atikorn PANYA Natthaporn PHONSATTA Amorntip MUANGPROM National Center for Genetic Engineering and Biotechnology Thailand Science ParkPathum Thani 12120Thailand Department of Biotechnology Faculty of Science and TechnologyThammasat UniversityPathum Thani 12120Thailand Institute of Crop Science and Nuclear Technology Utilization Zhejiang Academy of Agricultural SciencesHangzhou 310021P.R.China Khlong Luang Rice research Center Rice DepartmentPathum Thani 12120Thailand

Rice is the staple food for about half of the world’s population.Preferred by consumers,aromatic rice is a special type of rice with great commercial value.Cooking and eating qualities and aroma are the major grain qualities favored by most consumers.Currently,most of the available aromatic varieties have low yields and some undesirable agronomic traits.Thus,there is an urgent need to develop better aromatic rice varieties.This work aims to identify rice germplasm lines that have good grain quality and to develop new varieties with desirable traits.Thirty-six out of 188 germplasm lines were found to have betaine aldehyde dehydrogenase 2 (badh2) controlling the aroma and were analyzed for their 2-acetyl-1-pyrroline (2AP) contents.Then,17 of those lines were found to have alleles for low amylose content and low gelatinization temperature,controlled by waxy and starch synthase Ⅱa (SSⅡa),respectively,suggesting that they are aromatic rice lines with high cooking and eating qualities.A total of 158 F_(7)recombinant inbred lines (RILs) generated from five crosses of the selected germplasm lines were planted for phenotypic and yield observations,resulting in 27 F_(8)RILs selected for yield evaluation and genotyping.Finally,four out of the seven F_(9)aromatic RILs showed high yield,high 2AP production,and low amylose content,in agreement with their genotypes.The other three F9RILs were aromatic rice lines with high amylose content and high yield.Because consumer preferences for grain quality vary depending on regions and ethnic groups,the high-yielding aromatic RILs generated from this study can be used to increase the yield of Thai rice and to raise market value and farm profits.

关键词： germplasm functional marker aromatic rice badh2 waxy SSIIa RILs

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Applying singular value decomposition technique for quantifying the insects in commercial Thai Hommali Rice from NIR Spectrum

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Journal of Innovative Optical Health Sciences 2017年第2期10卷 93-104页

作者： Puttinun Jarruwat Prasan Choomjaihan Department of agricultural Engineering Faculty of Engineering King Mongkut's Institute of Technology Ladkrabang LadkrabangBangkokThailand10520

Insect infestation in rice stock is a significant issue in rice exporting business,resulting in the loss of product quality,nutrient as well as the economic losses.However,detecting the insect cont amination with the traditional sorting techniques were destructive,inaccurate,time consuming and unable to detect the internal insect infestation.This study used near infrared(NIR)spectroscopy for obtaining the absorbent spectra from the insect cont amination in two kinds of rice samples,Milled Hommali rice(MHR)and Brown Hommali rice(BHR).The mathematical methods of partial least squares(PLSs)regression and singular value de-composition(SVD)were employed to construct the predicting model.The statistical analysis results,R,RMSEP,RPD and bias,concluded that the predictive models from PLS for MHR and BHR were 0.95 and 0.90,0.014 and 0.019,4.79 and 3.11,as well as-0.007 and-0.008,respectively;while the statistical analysis results from SVD for MHR and BHR were 0.97 and 0.96,0.012 and 0.013,5.71 and 5.39,as well as-0.003 and 0.002,respectively.It showed that SVD technique performed better than PLS technique which shows that using the advantage of SVD technique required less amounts of wave numbers for predicting and was possible to construct the low cost handheld equipment for detecting the insects in rice samples.

关键词： Insect rice NIR SVD

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Stability and solubility improvement of Sompoi(Acacia concinna Linn.) pod extract by topical microemulsion

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Asian Journal of Pharmaceutical Sciences 2017年第4期12卷 386-393页

作者： Worrapan Poomanee Wantida Chaiyana R.Randall Wickett Pimporn Leelapornpisid Faculty of Pharmacy Chiang Mai UniversityChiang MaiThailand James L.Winkle College of Pharmacy University of CincinnatiCincinnatiUSA

The aim of this study was to enhance the solubility and stability of Acacia concinna extract by loading in a microemulsion for topical application. Both physical appearance and biological activities of the extract-loaded microemulsion were determined in comparison with the extract solution. Pseudoternary phase diagrams of three oil types including tea seed oil, grape seed oil, and sesame oil, together with polysorbate 85 or the mixture of polysorbate 85 and sorbitan oleate as surfactants, and absolute ethanol as a co-surfactant were constructed to optimize the microemulsion area. The selected microemulsion was then characterized for droplet size, polydispersity index, and viscosity. Tea seed oil exhibited the highest microemulsion area in the phase diagram because it had the highest unsaturated fatty acid content. The microemulsion composed of tea seed oil(5%), polysorbate 85(40%), ethanol(20%), and water(35%) exhibited Newtonian flow behavior with the droplet size and polydispersity index of 68.03 ± 1.09 nm and 0.44 ± 0.04, respectively. After 4% w/w of the extract was incorporated into the microemulsion, larger droplets size was observed(239.77 ± 12.69 nm)with a lower polydispersity index(0.37 ± 0.02). After storage in various conditions, both physical appearances and the stability of biological activity of the extract-loaded microemulsion were improved compared to the solution. Therefore, the A. concinna loaded microemulsion may be a promising carrier for further development into a topical formulation and clinical trials for pharmaceutical and cosmeceutical applications are also suggested.

关键词： Microemulsions Stability Acacia concinna Linn Antioxidant activity Lipid peroxidation Antityrosinase activity

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Molecular Markers and Candidate Genes for Thermo-Sensitive Genic Male Sterile in Rice

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Rice science 2019年第3期26卷 147-156,I0008-I0012页

作者： Sudthana KHLAIMONGKHON Sriprapai CHAKHONKAEN Keasinee PITNGAM Khanittha DITTHAB Numphet SANGARWUT Natjaree PANYAWUT Thiwawan WASINANON Chareerat MONGKOLSIRIWATANA Julapark CHUNWONGSE Amorntip MUANGPROM Center for agricultural Biotechnology Kasetsart University National Center for Genetic Engineering and Biotechnology National Science and Technology Development Agency Division of Genetics Faculty of Liberal Arts and Science Kasetsart University

The discovery of thermo-sensitive genic male sterility(TGMS) has led to development of a simple and highly efficient two-line breeding system. In this study, genetic analysis was conducted using three F_2 populations derived from crosses between IR68301 S, an indica TGMS rice line, and IR14632(tropical japonica), Supanburi 91062(indica) and IR67966-188-2-2-1(tropical japonica), respectively.Approximately 1:3 ratio between sterile and normal pollen of F_2 plants from the three populations revealed that TGMS is controlled by a single recessive gene. Bulked segregant analysis using simple sequence repeat(SSR) and insertion-deletion(InDel) markers were used to identify markers linked to the tms gene. The linkage analysis based on the three populations indicated that the tms locus was located on chromosome 2 covering the same area. Using IR68301S × IR14632 F_2 population, the results showed that the tms locus was located between SSR marker RM12676 and InDel marker 2gAP0050058. The genetic distance from the tms gene to these two flanking markers were 1.10 and 0.82 cM, respectively.InDel marker 2gAP004045 located between these two markers showed complete co-segregation with the TGMS phenotype. In addition, InDel marker vf0206114052 showed 2.94 cM linked to the tms gene using F_2 populations of IR68301S × Supanburi 91062. These markers are useful tool for developing new TGMS lines by marker-assisted selection. There were ten genes located between the two flanking markers RM12676 and 2gAP0050058. Using quantitative real-time PCR for expression analysis, 7 of the 10 genes showed expression in panicles, and response to temperatures. These genes could be the candidate gene controlling TGMS in IR68301S.

关键词： hybrid rice thermo-sensitive genic male sterility insertion/deletion simple sequence repeat marker-assisted selection

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Kinetics of Ligand-Controlled Release of Zinc in Acid Sulfate Paddy Soils

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Pedosphere 2019年第2期29卷 216-223页

作者： Worachart WISAWAPIPAT Aksarapak PONGPOM Department of Soil Science Faculty of Agriculture Kasetsart University

The elevated solubility of zinc(Zn) in acid sulfate paddy soils can limit rice production and pose a risk of environmental pollution.However, little attention has been paid to the ligand-controlled release of Zn in these soils. Here we quantified the rate of ligandcontrolled Zn release in Thai acid sulfate paddy soils, using ethylenediaminetetraacetic acid as the extractant. Sequential extractions were performed to obtain quantitative information on Zn fractions contributing to the ligand-controlled mechanisms. The amount of Zn released within 192 h varied significantly(8–43 mg kg^(-1)), which corresponded to 12%–40% of the total soil Zn, indicating that Zn solubility in most soils was relatively low and that Zn mainly occurred as residual phases. The kinetics of Zn release was well described by the power function model(r = 0.65–0.99, median = 0.87). The magnitude of initial Zn release(coefficient a) was significantly(P < 0.05) related to the aqua regia-soluble Zn. Easily mobile Zn, organically bound Zn, and Zn associated with Fe and Mn oxides also contributed to the ligand-controlled release mechanisms to various degrees. Our results provide a systematic understanding of Zn fractions and release from acid sulfate paddy soils, the dynamics of which have a significant influence on the availability, phytoextraction, and mobility of Zn in terrestrial and engineered environments.

关键词： ethylenediaminetetraacetic acid kinetic release ligand exchange sequential extraction zinc fraction zinc solubility

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Metabolic disturbance and phytochemical changes in Andrographis paniculata and possible action mode of andrographolide

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Asian Pacific Journal of Tropical Biomedicine 2018年第2期8卷 85-91页

作者： Netiya Karaket Nuchada Maneejantra Patoomratana Tuchinda Jirapna Kunapin Choowong Auesukaree Kanyaratt Supaibulwatana School of Interdisciplinary Studies Mahidol University Kanchanaburi Campus Department of Biotechnology Faculty of Science Mahidol University Department of Chemistry Faculty of Science Mahidol University Department of Biology Faculty of Science Mahidol University

Objecive: To explore the effect of gibberellic acid(GA_3) and its inhibitor paclobutrazol(PBZ)on chemical composition and their pharmacological effects on Andrographis paniculata(Burm.f.) Wall. ex Nees, and to clarify action mode of andrographolide.Methods: The chemical composition was extracted by sequential extraction with hexane, dichloromethane, ethyl acetate and methanol, respectively. Andrographolide and its derivatives were evaluated by HPLC. Moreover, the metabolic profiling was analyzed by GC-MS. Inhibitory effect of crude extracts was tested against Staphylococcus aureus using agar well diffusion method. Mode of action was tested against mutant yeast by spotting assay. Andrographolide were tested for their mode of action against eukaryotes. Rsults: Among different solvents, dichloromethane gave the highest yield of crude(3.58% DW), with the highest andrographolide content(8.3 mg/g DW). The effect of plant hormone(10 mg/L GA_3 or PBZ) on phytochemical variations and bioactivity of Andrographis paniculata was demonstrated. It was found that PBZ promoted sesquiterpene compounds about 3.5 times over than GA_3 treatment. But inhibitory effect of extracts against Staphylococcus aureus was highest in GA_3 treated plants; andrographolide and 14-deoxy-11,12-didehydroandrographolide contents were significantly higher than those of water or PBZ. It was found that there were 11 strains involving in ergosterol biosynthesis, V-ATPase activity and homeostasis, and superoxide detoxification process. In this regard, andrographolide might cause the damage on the lipid bilayer of yeast cell and plasma membrane by interfering ergosterol biosynthesis.Conclusions: It is found that GA_3 promotes andrographolide and 14-deoxy-11,12-didehydroandrographolide content while PBZ promotes sesquiterpene content. Andrographolide might cause the damage on the lipid bilayer of yeast cell and plasma membrane by interfering ergosterol biosynthesis. It might also affect mitochondria electron transport chain, leading to the occurrence of ROS

关键词： Andrographis paniculata Andrographolide Cell elicitation Gibberellic acid Paclobutrazol Mode of action

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Genetic Diversity and Allelic Frequency of Selected Thai and Exotic Rice Germplasm Using SSR Markers

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Rice science 2019年第6期26卷 393-403页

作者： Wanwarang PATHAICHINDACHOTE Natjaree PANYAWUT Kannika SIKAEWTUNG Sujin PATARAPUWADOL Amorntip MUANGPROM Center for agricultural Biotechnology Kasetsart University Kamphaeng Saen Campus Nakhon Pathom 73140 Thailand Center of Excellence on agricultural Biotechnology Science and Technology Postgraduate Education and Research Development Office Office of Higher Education Commission Ministry of Education Bangkok 10900 Thailand Department of agricultural Sciences Faculty of Agriculture Natural Resources and Environment Naresuan University Phitsanulok 65000 Thailand National Center for Genetic Engineering and Biotechnology Thailand Science Park Pathum Thani 12120 Thailand Department of Plant Pathology Faculty of Agriculture at Kamphaeng Saen Kasetsart University Kamphaeng Saen Campus Nakhon Pathom 73140 Thailand

A collection of 167 Thai and exotic rice accessions was subjected for evaluation of genetic diversity and assessment of relationship by simple sequence repeat (SSR) markers. Among a total of 49 SSR markers, 13 markers distributing over 12 rice chromosomes showed clear polymorphic band patterns, and they were selected for genetic assessment. A total of 110 alleles were detected with an average of 8.46 alleles per locus. The averages of gene diversity, heterozygosity and polymorphic information content were 0.59, 0.02 and 0.56, respectively. The unweighted-pair group method with arithmetic averages (UPGMA) clustering analysis was performed for genetic distance, and phylogenetic tree was constructed. The result showed that this rice collection was divided into two major groups, classified as japonica and indica subspecies. Within the japonica group, temperate japonica and tropical japonica subgroups can be clearly separated. Three-dimensional principal component analysis projection and model-based population structure analysis showed consistent clustering results with two major groups of UPGMA analysis, supporting the classification of japonica and indica subspecies. The indica allelic frequency was also investigated to provide an indicative guide for breeders to overcome the practical problems on sterility of inter-subspecies hybrid offspring. This rice collection and information obtained in this study will be useful for rice breeding programs.

关键词： rice genetic diversity indica japonica allelic frequency simple sequence repeat

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Physico-mechanical characterization of polyurethane foam dressings containing natural polyols

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Asian Journal of Pharmaceutical Sciences 2016年第1期11卷 114-115页

作者： Nantaporn Namviriyachote Kitipun Manosittisak Garnpimol C.Ritthidej Department of Pharmaceutics and Industrial Pharmacy Faculty of Pharmaceutical SciencesChulalongkorn UniversityBangkok 10330Thailand Bangkok Botanica Co. Ltd.Bangkok 10600Thailand

Advanced wound dressings are replaced traditional dressings in order to provide appropriate environment for healing and also to promote cell migration[1].The key characteristics of wound dressings are non-toxic,highly absorbed,air permeable,biocompatible and have good mechanical properties[2].Due to providing moist environment and also protecting maceration at the wound edge area,foam dressings are used in various clinical applications[3].They are mostly prepared from polyurethane between polyols and isocyanate polymerization.

关键词： Polyurethane foam Natural polyols Starch Physico-mechanical properties

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