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Expression of Notch signal in rat model of chronic periodontitis

Journal of Hainan Medical University 2019年第6期25卷 1-5页

作者： Li Zhang Qi-Ya Fu Bao-Liang Wang School of Stomatology Hainan Medical UniversityHaikou571101Hainan ProvinceChina Department of Orthodontics Guanghua Stomatological HospitalHaikou570203Hainan ProvinceChina Department of Stomatology Linyi People HospitalLinyi276000Shandong ProvinceChina

Objective: To establish a chronic periodontitis model of SD rats and detect the expression of Notch in peripheral blood mononuclear cells (PBMCs) of chronic periodontitis model was detected by real-time quantitative polymerase chain reaction (RT-PCR), intracellular staining and flow cytometry. It is hoped that we can find out the potential significance of Notch related molecules in the development of chronic periodontitis and provide a theoretical basis for seeking new methods of treating chronic periodontitis. Methods: 27 male SD rats aged 6-8 weeks were randomly divided into control group and model group. By means of Porphyromonas gingivalis, a chronic periodontitis rat model was established. The gingival index, tooth mobility and periodontal pocket depth were recorded. The expression levels of Notch signaling related receptors (Notch 1 to Notch 4) in PBMC of model mice were detected by fluorescence quantitative polymerase chain reaction, flow cytometry and intracellular staining at different levels and compared with the control group. Results: The model of chronic periodontitis in SD rats was successfully established. The expression of Mrna of Notch 1, Notch 3, Notch 4 and percentage of cell expression in the model group were significantly lower than that in the control group (P2 Mrna and percentage of cell expression were significantly higher (P<0.05). Conclusion: This indicated that Notch and its ligands may play important roles in imbalance of immune balance for chronic periodontitis and provide ideas for in-depth study of the pathogenesis of chronic periodontitis.

关键词： Notch gene Chronic periodontitis Animal model

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