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Assembly and network of Rhei Radix et Rhizoma surface microbiome shaped by processing methods and sampling locations

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Chinese Herbal Medicines 2025年第1期17卷 189-199页

作者： Guangfei Wei Xiao Chen Guozhuang Zhang Conglian Liang Zhaoyu Zhang Bo Zhang Shilin Chen Linlin Dong State Key Laboratory for Quality Ensurance and Sustainable Use of Dao-di Herbs Institute of Chinese Materia Medica China Academy of Chinese Medical Sciences School of Biomedicine Beijing City University Shandong University of Traditional Chinese Medicine School of Pharmacy Linyi University Institute of Herbgenomics Chengdu University of Traditional Chinese Medicine

Objective:Rhei Radix et Rhizoma has five types of products,namely,raw rhubarb(RR),wine rhubarb(WR),vinegar rhubarb(VR),cooked rhubarb(CR),and rhubarb charcoal(RC).However,Rhei Radix et Rhizoma is easily contaminated with fungi and mycotoxins if not harvested or processed ***,we intend to analyze how microbiome assemblies and co-occurrence patterns are influenced by sampling locations and processing ***:High-throughput sequencing and internal transcribed spacer 2(ITS2) were carried out to study the diversities(α-and β-diversity),composition(dominant taxa and potential biomarkers),and network complexitity of surface fungi on RR, WR,VR,CR,and RC collected from Gansu and Sichuan provinces,***:The phyla Ascomycota and Basidiomycota;the genera Kazachstania,Malassezia,and Asterotremella;and the species Kazachstania exigua,Asterotremella pseudolonga,and Malassezia restricta were the dominant fungi and exhibited differences in the two provinces and the five processed *** α-diversity and network complexity were strongly dependent on processing *** 1,the Shannon index,and network complexity and connectivity were highest in the CR *** α-diversity and network complexity were influenced by sampling *** 1 and network complexity and connectivity were highest in the Gansu ***:The assembly and network of the surface microbiome on Rhei Radix et Rhizoma were shaped by processing methods and sampling *** paper offers a comprehensive understanding of microorganisms,which can provide early warning for potential mycotoxins and ensure the safety of drugs and consumers.

关键词： community assembly fungi processing methods Rhei Radix et Rhizoma sampling locations

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Research achievements and application in anaerobic treatment of organic solid wastes—A review

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Chinese Journal Of Geochemistry 2006年第2期25卷 178-181页

作者：周富春熊德国鲜学福徐龙君 Key Laboratory of the Exploitation of Southwestern Resources & the Environmental Disaster Control Engineering under the State Commission of Education Chongqing University College of Hehai Chongqing Jiaotong University Chongqing 400074 China Jiaozuo Industry University Jiaozuo 454000 China

Anaerobic digestion is a good method, which possesses the optimal combination of volume reduction, probability of success and potential for resource and energy recovery. However, relatively little research has been done on the anaerobic digestion of organic solid wastes (OSW), especially in China. However, different substrates, start-up conditions, micro-organisms, processing technologies, pre-treatment methods could influence the result of anaerobic digestion. Anaerobic treatment of municipal OSW is less than that of wastewaters because some problems and obstructions need to be solved. Meanwhile, the application of anaerobic digestion of OSW is also discussed in the present paper.

关键词：有机固体废物厌氧性处理环境污染环境处理

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Progress and perspective on cotton breeding in Pakistan

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Journal of Cotton Research 2022年第4期5卷 25-41页

作者： SHAHZAD Kashif MUBEEN Iqra ZHANG Meng ZHANG Xuexian WU Jianyong XING Chaozhu State Key Laboratory of Cotton Biology/Key Laboratory of Biological and Genetic Breeding of Cotton Ministry of Agriculture and Rural Affairs/Institute of Cotton ResearchChinese Academy of Agricultural ScienceAnyang 455000HenanChina Centre of Agricultural Biochemistry and Bio-technology University of AgricultureFaisalabad 38040Pakistan

Cotton is the prime natural fiber with economic significance *** farming and breeding have a long his-tory in *** development of high yielding upland cotton(Gossypium hirsutum)varieties gradually replaced the cultivation of diploid Gossypium *** change along with emergence of new epidemic diseases caused yield loss in recent *** biotic stress considerably reduced the performance and yield potential of ***-able breeding strategies are essential to generate useful genetic variations and to identify desired *** breeding has remarkably increased cotton yield and fiber quality,which has cultivated the NIAB-78,S-12,MNH‐786,and FH‐Lalazar like ***,this phenotypic selection based breeding method has low efficiency to pro-duce stress resilient *** efficiency of traditional breeding has significantly improved by the marker assisted selection *** in molecular genetics,bioinformatics analysis,genetic engineering,and genome sequencing have opened new technique routes for cotton *** addition,genetic improvement through quantitative trait loci,transcriptome,and CRISPR/Cas9 mediated genomic editing can provide suitable platform to improve the resistance to stresses induced by bollworms,cotton leaf curl virus,heat,drought,and *** approval of transgenic lines harboring triple gene Cry1Ac+Cry2A+GTG are critical for cotton *** review has critically discussed the progress and limitations of cotton breeding in Pakistan,and reviewed the utilization of novel genetic variations and selection tools for sustainable cotton production.

关键词： Cotton yield Biotic stress Abiotic stress Conventional breeding Molecular marker Genome selection

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Comparative performance of hybrid generations reveals the potential application of F_(2)hybrids in upland cotton

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Journal of Cotton Research 2022年第2期5卷 193-206页

作者： CHEN Liangliang TANG Huini ZHANG Xuexian QI Tingxiang GUO Liping SHAHZAD Kashif WANG Hailin QIAO Xiuqin ZANG Rong ZHANG Meng WU Jianyong XING Chaozhu State Key Laboratory of Cotton Biology In stitute of Cotton Research of Chinese Academy of Agricultural SciencesKey Laboratory for Cotton Genetic ImprovementMinistry of Agriculture and Rural AffairsAnyang 455000HenanChina

Background:The utilization of heterosis has greatly improved the productivity of cotton ***,a major constraint for the large-scale promotion of F_(1) hybrid cotton is artificial emasculation and *** study proposed the potential utilization of F_(2) hybrids to improve upland cotton production through a comparative evaluation of hybrid ***:Eight upland cotton varieties were analyzed and crosses were made according to NCII incomplete diallel cross-breeding design in two cotton belts of *** analysis revealed significant differences in agronomic,yield,and fiber quality in both generations and *** broad-sense heritability of agronomic and yield traits was relatively higher than quality ***,the narrow-sense heritability of some traits was higher in F_(2) than in the F_(1) generation in both cotton ***,parental lines Zhong901,ZB,L28,and Z98 were observed with maximum combining ability while combinations with strong special combining ability were ZB×DT,L28×Z98,and ZB×*** yield traits heterosis was predominant in both ***,the level of heterosis was altered with trait,hybrid combination,generation,and ***,L28×Z98 performed outstandingly in *** lint yield(LY)was 24.2%higher in F_(1) and 11.6%in F_(2) than that of the control Ruiza *** performance of SJ48×Z98 was excellent in Aral which showed 36.5%higher LY in F_(2)and 10.9%in F_(2)than control CCRI *** results revealed most hybrid combinations had shown a low level of heterosis for agronomic and fiber quality traits in both ***,ZB×DT and L28×Z98 showed hybrid vigor for multiple traits in both generations and cotton *** is feasible to screen strong heterosis hybrid combinations with fine fiber in early *** the two environments,the correlation of some traits showed the same trend,and the correlation degree of Anyang site was higher than that

关键词： Upland cotton F_(2)generation Combining ability Heterosis Heritability

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Transcriptome analysis reveals immune-related genes in tissues of Vibrio anguillarum-infected turbot Scophthalmus maximus

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Journal of Oceanology and Limnology 2024年第1期42卷 332-344页

作者： Yuting SONG Maqsood Ahmed SOOMRO Xianzhi DONG Guobin HU College of Marine Life Sciences Ocean University of ChinaQingdao 266003China Institute of Evolution&Marine Biodiversity Ocean University of ChinaQingdao 266003China Institute of Biophysis Chinese Academy of SciencesBeijing 100101China

Turbot Scophthalmus maximus is an important mariculture fish species with high economic ***,the bacterial diseases caused by Vibrio anguillarum infection bring huge economic losses to the turbot aquaculture *** understand the immune response of the turbot against *** infection and to explore novel immune-related genes,the transcriptome analysis of turbot spleen and gills were conducted after *** *** expressed genes(DEGs)were identified in spleen and gill of the turbot amounted to 17261 and 16436,respectively.A large number of immunerelated DEGs were enriched in cytokine-cytokine receptor interaction signaling pathway,and the others by the kyoto encyclopedia of genes and genomes(KEGG)*** gene ontology(GO)classification analysis revealed that *** infection had the greatest effect on biological processes and cellular *** immune-related DEGs were identified in the spleen(cstl.1,egfl6,lamb21,v2rx4,calcr,and gpr78a)and gills(ghra,sh3gl2a,cst12,inhbaa,cxcl8,and il-1b)by heat *** proteinprotein interaction(PPI)networks were constructed to analyze the immune *** results demonstrate that the maturation and antigen processing of major histocompatibility complex(MHC)class II molecule,and calcitonin-or adrenomedullin-regulated physiological activity were important events in the immunity of turbot against *** *** the gills,the protein interactions in TGF-βsignaling pathway,production of inflammatory factors,and endocytosis regulation were most *** research laid a foundation for discovering novel immune-related genes and enriching the knowledge of immune mechanisms of turbot against *** infection.

关键词： Scophthalmus maximus Vibrio anguillarum transcriptome differentially expressed genes immune mechanism

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Identification and analysis of serum samples by surface-enhanced Raman spectroscopy combined with characteristic ratio method and PCA for gastric cancer detection

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Journal of Innovative Optical Health Sciences 2019年第2期12卷 13-23页

作者： Liu Guo Yuanpeng Li Furong Huang Jia Dong Fucui Li Xinhao Yang Siqi Zhu Maoxun Yang Department of Opto-Electronic Engineering Jinan University Guangzhou 510632P.R.China Zhuhai Da Hengqin Science and Technology Development Co. Ltd Hengqin New AreaZhuhai 519000P.R.China Zhuhai Hopegenes Medical&Pharmaceutical Institute Co. Ltd Hengqin New AreaZhuhai 519000P.R.China Research Institute of Jinan University in Dongguan Dongguan 523000 P.R.China

This study aimed to explore the application of surface-enhanced Raman scattering(SERS)in the rapid diagnosis of gastric *** SERS spectra of 68 serum samples from gastric cancer patients and healthy volunteers were *** characteristic ratio method(CRM)and principal component analysis(PCA)were used to differentiate gastric cancer serum from normal *** with healthy volunteers,the serum SERS intensity of gastric cancer patients was relatively high at 722 cm^(-1),while it was relatively low at 588,644,861,1008,1235,1397,1445 and 1586 cm^(-1).These results indicated that the relative content of nucleic acids in the serum of gastric cancer patients rises while the relative content of amino acids and carbohydrates *** PCA,the sensitivity and specificity of discriminating gastric cancer were 94.1%and 94.1%,respectively,with the accuracy of 94.1%.Based on the intensity ratios of four characteristic peaks at 722,861,1008 and 1397 cm^(-1),CRM presented the diagnostic sensitivity and specificity of 100%and 97.4%,respectively,and the accuracy of 98.5%.Therefore,the three peak intensity ratios of I_(722)/I_(861),I_(722)/I_(1008)and I_(722)/I_(1397)can be considered as biologicalfingerprint information for gastric cancer diagnosis and can rapidly and directly reflect the physiological and pathological changes associated with gastric cancer *** study provides an important basis and standards for the early diagnosis of gastric cancer.

关键词： Surface-enhanced Raman spectroscopy serum gastric cancer characteristic ratio method principal components analysis

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Seismic airgun exploration of continental crust structures

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Science China Earth Sciences 2017年第10期60卷 1739-1751页

作者： CHEN Yong WANG BaoShan YAO HuaJian School of Earth Sciences and Engineering Nanjing University Nanjing 210046 China Institute of Earthquake Science China Earthquake Administration Beijing 100036 China Key Laboratory of Seismic Observation and Geophysical Imaging Institute of Geophysics China Earthquake Administration Beijing 100081 China Laboratory of Seismology and Physics of Earth's Interior School of Earth and Space Sciences University of Science and Technology of China Hefei 230026 China National Geophysical Observatory at Mengcheng University of Science and Technology of China Hefei 230026 China

The active sources generate seismic waves transmitting appropriate through the deep is underground key and can be used to image Abstract high-resolution subsurface ***,an seismic source the factor to active source *** order to study the structure of continental crust and its temporal variations,we selected an artificial seismic source generated from releasing air bubbles in water(airgun source hereinafter)out of a variety and of artificial sources like the is explosion,new electronic sparkers,source hammering,eccentric proven vibration,be heavy-duty train vibration,vibroseis *** Three source Fixed a type of artificial that have been to environmentally friendly,safe,and highly *** western Signal China Transmission and Stations(FASTS)have been for built a few years ago in Yunnan,Xinjiang,and Gansu provinces in have been continuously them running several *** waves generated away by the the airgun sources are highly seismic reproducible waves and stacking in of can produce can good seismograms on 1300 stations km far from source,for instance,an produced Xinjiang FASTS be well about recorded 60 nearly away after 5000 stacking,China covering area of 6 million km2 and penetrating down to of a depth of *** about 10 FASTSs in would enable long-term illuminate continuous subsurface underground structures,monitoring can all 9.6 million km2 of land *** from airgun sky active sources as lanterns to we achieve the situation with"Beidou surveys the and lantern illuminates underground".

关键词： Continental crust structure Airgun Green Environmental protection Seismic surveys

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Basic Features of the Crustal Structure in the Lower Yangtze and Its Neighboring Area in the Chinese Mainland： Review of Deep Seismic Sounding Research

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Earthquake Research in China 2016年第3期30卷 298-315页

作者： Bai Zhiming Wu Qingju Xu Tao Wang Xiao State Key Laboratory of Lithosphere Evolution Institute of Geology and Geophysics CAS Beijing 100029 China Institute of Geophysics China Earthquake Administration Beijing 100081 China CAS Center for Excellence in Tibetan Plateau Earth Sciences Beijing 100101 China University of Chinese Academy of Sciences Beijing 100049 China

The Deep Seismic Sounding( DSS) projects carried out from the 1970 s in the lower Yangtze region and its neighboring area were reviewed in this paper,then the basic wave group features of those wide angle reflection / refraction record sections,and of the crustal structure are summarized. It shows that there were in total five clear wave groups on the record sections,which include the first arrival Pg,the reflection P1 from the bottom interface of the upper crust,the reflection P3 from the bottom interface of the middle crust,the strong reflection Pm from the Moho boundary,and the refraction Pn from uppermost mantle. In general,these phases are easily consistently traced and compared,despite some first arrivals being delayed or arriving earlier than normal due to the shallow sedimentary cover or bedrocks. In particular,in the Dabie Mountain region the seismic events of a few gathered shots always have weak reflection energy,are twisted,or exhibit disorganized waveforms, which could be attributed to the disruption variations of reflection depth,the broken Moho,and the discontinuity of the reflection boundary within crust. The regional crustal structures are composed of the upper,middle and lower crust,of which the middle and lower layers can be divided into two weak reflection ones. The crustal thickness of the North China and Yangtze platform are 30km- 36 km,and the Moho exhibits a flat geometry despite some local uplifts. The average pressure velocity in lower crust beneath this two tectonic area is 6. 7 ± 0. 3km / s. Nevertheless,beneath the Dabieshan area the crustal thickness is 32km- 41 km,the Moho bends down sharply andtakes an abrupt 4km- 7km dislocation in the vertical direction. The average pressure velocity in the lower crust beneath the Dabieshan area is 6. 8 ± 0. 2km / s.

关键词： Deep seismic sounding projects Wave group feature Crustal structure feature Anhui Province and its neighboring area

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Identification and Expression Analysis of Abscisic Acid Signal Transduction Genes in Hemp Seeds

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Phyton-International Journal of Experimental Botany 2023年第7期92卷 2087-2103页

作者： Cong Hou Kang Ning Xiuye Wei Yufei Cheng Huatao Yu Haibin Yu Xia Liu Linlin Dong School of Chemistry Chemical Engineering and Life SciencesWuhan University of TechnologyWuhan430070China Yunnan Hemp Industrial Investment Co. Ltd.Kunming650000China

Abscisic acid(ABA)is involved in regulating diverse biological processes,but its signal transduction genes and roles in hemp seed germination are not well ***,the ABA signaling pathway members,PYL,PP2C and SnRK2 gene families,were identified from the hemp reference genome,including 7 CsPYL(pyrab-actin resistance1-like,ABA receptor),8 CsPP2CA(group A protein phosphatase 2c),and 7 CsSnRK2(sucrose nonfermenting1-related protein kinase 2).The content of ABA in hemp seeds in germination stage is lower than that in non-germination *** ABA(1 or 10μM)treatment had a significant regulatory effect on the selected PYL,PP2C,SnRK2 gene ***3 and CsHAI1 were most significantly affected by exogenous ABA *** two-hybrid experiments were performed to reveal that CsPYL5,CsSnRK2.2,and CsSnRK2.3 could interact with CsPP2CA7 and demonstrate that this interaction was *** results indicated that CsPYL5,CsSnRK2.2,CsSnRK2.3 and CsPP2CA7 might involve in the ABA signaling transduction pathway of hemp seeds during the hemp seed germination *** study suggested that novel genetic views can be brought into investigation of ABA signaling pathway in hemp seeds and lay the foundation for further exploration of the mechanism of hemp seed germination.

关键词： Hemp seeds abscisic acid seed germination PYL-PP2C-SnRK2 gene expression

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lncR-GAS5 upregulates the splicing factor SRSF10 to impair endothelial autophagy,leading to atherogenesis

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Frontiers of Medicine 2023年第2期17卷 317-329页

作者： Yuhua Fan Yue Zhang Hongrui Zhao Wenfeng Liu Wanqing Xu Lintong Jiang Ranchen Xu Yue Zheng Xueqing Tang Xiaohan Li Limin Zhao Xin Liu Yang Hong Yuan Lin Hui Chen Yong Zhang Department of Pharmacology State–Province Key Laboratories of Biomedicine-Pharmaceutics of ChinaKey Laboratory of Cardiovascular Medicine ResearchMinistry of EducationCollege of PharmacyHarbin Medical UniversityHarbin 150081China Department of Pathology and Pathophysiology College of Basic Medical SciencesHarbin Medical University-DaqingDaqing 163319China Center for Drug Research and Development Guangdong Pharmaceutical UniversityGuangzhou 510006China

Long noncoding RNAs(lncRNAs)play a critical role in the regulation of ***,we investigated the role of the lncRNA growth arrest-specific 5(lncR-GAS5)in *** found that the enforced expression of lncR-GAS5 contributed to the development of atherosclerosis,which presented as increased plaque size and reduced collagen ***,impaired autophagy was observed,as shown by a decreased LC3II/LC3I protein ratio and an elevated P62 level in lncR-GAS5-overexpressing human aortic endothelial *** contrast,lncR-GAS5 knockdown promoted ***,serine/arginine-rich splicing factor 10(SRSF10)knockdown increased the LC3II/LC3I ratio and decreased the P62 level,thus enhancing the formation of autophagic vacuoles,autolysosomes,and ***,lncR-GAS5 regulated the downstream splicing factor SRSF10 to impair autophagy in the endothelium,which was reversed by the knockdown of *** results revealed that overexpression of the lncR-GAS5-targeted gene miR-193-5p promoted autophagy and autophagic vacuole accumulation by repressing its direct target gene,***,miR-193-5p overexpression decreased plaque size and increased collagen ***,these findings demonstrate that lncR-GAS5 partially contributes to atherogenesis and plaque instability by impairing endothelial *** conclusion,lncR-GAS5 overexpression arrested endothelial autophagy through the miR-193-5p/SRSF10 signaling ***,miR-193-5p/SRSF10 may serve as a novel treatment target for atherosclerosis.

关键词： lncR-GAS5 miR-193-5p splicing factor SRSF10 autophagy atherogenesis

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