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3nce upon a Time- Chloroplast protein Imporl Research from Infancy to Future Challenges

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Molecular Plant 2016年第6期9卷 798-812页

作者： Bettina Bolter Jurgen Soill Department Biotogie I-Botanik Ludwig-Maximilians-Universitat GroBhaderner Stral3e 2-4 82152 Planegg-Martinsried Germany Munich center for Integrated protein Science CiPSM Ludwig-Maximilians-Universitat Feodor-Lynen-Strasse 25 81377 Munich Germany

protein import into chloroplasts has been a focus of research for several decades. The first publications dealing with this fascinating topic appeared in the 1970s. From the initial realization that many plastid proteins are being encoded for in the nucleus and require transport into their target organelle to the identification of import components in the cytosol, chloroplast envelopes, and stroma, as well as elucidation of some mechanistic details, more fascinating aspects are still being unraveled. With this overview, we present a survey of the beginnings of chloroplast protein import research, the first steps on this winding road, and end with a glimpse into the future.

关键词： chloroplast protein import historical perspective Toc Tic evolution envelope membranes

Research journey of respirasome

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protein & Cell 2020年第5期11卷 318-338页

作者： Meng Wu Jinke Gu Shuai Zong Runyu Guo Tianya Liu Maojun Yang Ministry of Education Key Laboratory of protein Science Tsinghua-Peking Joint Center for Life SciencesBeijing Advanced Innovation Center for Structural BiologySchool of Life SciencesTsinghua UniversityBeijing 100084China School of Pharmacy Tongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China

Respirasome,as a vital part of the oxidative phosphorylation system,undertakes the task of transferring electrons from the electron donors to oxygen and produces a proton concentration gradient across the inner mitochondrial membrane through the coupled translocation of *** research has been carried out on this lynchpin of *** the discovery of individual respiratory complexes to the report of the high-resolution structure of mammalian respiratory supercomplex I1III2IV1,scientists have gradually uncovered the mysterious veil of the electron transport chain(ETC).With the discovery of the mammalian respiratory mega complex I2III2IV2,a new perspective emerges in the research field of the *** these advances glitters the light of the revolution in both theory and ***,we give a short review about how scientists‘see’the structure and the mechanism of respirasome from the macroscopic scale to the atomic scale during the past decades.

关键词： electron transport chain supercomplex organization cellular respiration structure of respirasome cryo-EM megacomplex

Cu/Co/Cr Nanocomposites Obtained from Hydrotalcite Precursors as Catalysts for Thermal Decomposition of Ammonium Perchlorate

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Acta Metallurgica Sinica(English Letters) 2014年第2期27卷 211-216页

作者： Hongbo Liu Zhiyong Huang Bingzhi Guo Qingze Jiao Zhiyong Xiong School of Chemical Engineering and Materials Science Beijing Institute of Technology Zhuhai School of Chemical Engineering and Environment Beijing Institute of Technology College of Light Industry and Food Sciences South China University of Technology

A series of Cu/Co/Cr nanocomposites with different Cu/Co/Cr molar ratios were obtained by calcination of Cu/ Co/Cr hydrotalcites precursors, which were prepared by a co-precipitation reaction. X-ray diffraction, inductively coupled plasma analysis, and transmission electron microscopy were used to characterize the structure, composition, and mor- phology of Cu/Co/Cr nanocomposites. The results show that Cu/Co/Cr nanocomposites have both CuCr204 and CoCr204 spinel phase. The particle size of Cu/Co/Cr nanocomposites is 15-20 nm and the specific surface area is 95-115 m2/g. Cu/ Co/Cr nanocomposites were used as new catalysts for improving thermal decomposition of ammonium perchlorate （AP）. Their catalytic activities were investigated using differential thermal analysis and thermal gravimetric analyzer coupled with an online mass spectrometer. The results show that the decomposition temperature of AP lowered 132-146℃ by adding 4 wt% Cu/Co/Cr nanocomposites. Catalytic activities of the prepared nanocomposites depend on the calcinations temperature and addition amount of corresponding nanocomposites. The possible catalytic mechanism of Cu/Co/Cr nanocomposites was also studied and discussed.

关键词： Nanocomposite Thermal decomposition Catalytic activities 1

Conformational dynamics in GPCR signaling by NMR

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Magnetic Resonance Letters 2022年第3期2卷 139-146页

作者： Yunfei Hu Changwen Jin Joint Laboratory of the National centers for Magnetic Resonance in Wuhan and in Beijing Wuhan430071China Beijing Nuclear Magnetic Resonance center College of Chemistry and Molecular EngineeringPeking UniversityBeijing100871China State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics National Center for Magnetic Resonance in WuhanKey Laboratory of Magnetic Resonance in Biological SystemsInnovation Academy for Precision Measurement Science and TechnologyChinese Academy of SciencesWuhan430071China

G-protein-coupled receptors(GPCRs)mediate a wide range of cellular responses to various ligands or stimuli,and are the most important drug targets associated with human *** major advances in GPCR structural biology have greatly deepened our understanding of its activation mechanism,the highly complex changes in the structural dynamics of GPCRs during activation remain underdetermined and their links to physiological functions largely *** nuclear magnetic resonance(NMR)spectroscopy is an essential technique that allows the characterization of protein structural dynamics at atomic level,and has been applied in the studies of GPCR structural-function relationship in the past ***,we summarize a few specific studies in which solution NMR methods were employed and provided novel insights into questions difficult to be addressed by other methods.

关键词： G-protein-coupled receptor(GPCR) Membrane protein Dynamics Nuclear magnetic resonance(NMR) Cell signaling

Natural variation in the transcription factor REPLUMLESS contributes to both disease resistance and plant growth in Arabidopsis

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Plant Communications 2022年第5期3卷 186-198页

作者： Miqi Xu Xuncheng Wang Jing Liu Aolin Jia Chao Xu Xing Wang Deng Guangming He School of Life Sciences and School of Advanced Agricultural Sciences State Key Laboratory of Protein and Plant Gene ResearchPeking-Tsinghua Center for Life SciencesPeking UniversityBeijing 100871China Beijing Key Laboratory of Environment Friendly Management on Fruit Diseases and Pests in North China Institute of Plant ProtectionBeijing Academy of Agriculture and Forestry SciencesBeijing 100097China

When attacked by pathogens,plants need to reallocate energy from growth to defense to fend off the invaders,frequently incurring growth *** phenomenon is known as the growth–defense tradeoff and is orchestrated by a hardwired transcriptional *** key factors involved in this network has the potential to increase disease resistance without growth or yield loss,but the mechanisms underlying such changes require further *** conducting a genome-wide association study(GWAS)of leaves infected by the hemi-biotrophic bacterial pathogen Pseudomonas syringae ***(Pst)DC3000,we discovered that the Arabidopsis transcription factor REPLUMLESS(RPL)is necessary for bacterial *** importantly,RPL functions in promoting both disease resistance and *** analysis revealed a cluster of genes in the GRETCHEN HAGEN 3(GH3)family that were significantly upregulated in rpl mutants,leading to the accumulation of indole-3-acetic acid-aspartic acid(IAA-Asp).Consistent with this observation,transcripts of virulence effector genes were activated by IAA-Asp accumulated in the rpl *** found that RPL protein could directly bind to GH3 promoters and repress their *** also repressed flavonol synthesis by directly repressing CHI expression and thus activated the auxin transport pathway,which promotes plant ***,RPL plays an important role in plant immunity and functions in the auxin pathway to optimize Arabidopsis growth and defense.

关键词： natural variation GWAS REPLUMLESS bacterial resistance auxin

Biomimetic albumin-modified gold nanorods for photothermo-chemotherapy and macrophage polarization modulation

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Acta Pharmaceutica Sinica B 2018年第1期8卷 74-84页

作者： Dongdong Li Meng Zhang Fan Xu Yingzhi Chen Binfan Chen Ya Chang Huihai Zhong Hongyue Jin Yongzhuo Huang Shanghai Institute of Materia Medica Chinese Academy of Sciences Nano Sci-Tech Institute University of Science and Technology of China Shanghai University College of Sciences

Nanotechnology-based photothermal therapy has attracted great attention in the past decade. Nevertheless, photothermal therapy has some inherent drawbacks, such as the uneven heat production and limited laser penetration, often leading to insufficient treatment outcomes. Here, we developed a combination strategy to improve cancer therapy. The biomimetic albumin-modified gold nanorods(AuNRs) were prepared with incorporation of paclitaxel(PTX). This therapeutic system was characterized by several features. First, the albumin modification enhanced the biocompatibility and colloidal stability. Second, the surface-coated albumin promoted cellular uptake via the albumin-binding protein pathway. Third, PTX was incorporated via hydrophobic interaction between PTX and the albumin lipophilic domain. Fourth, the system can be used for combined photothermo-chemotherapy for yielding synergistic effects. The antitumor activity of the system was evaluated both in vitro and in vivo using the HCT116 colon cancer cell and tumor model. The combination therapy was found with an enhanced treatment efficiency and no obvious side effect. Most importantly, the thermal effect was also discovered with the ability to modulate the tumor microenvironments and suppress the macrophages polarization towards the M2 pro-tumor phenotype. It could be a mechanism for photothermal immunotherapy. The combination strategy and the system provide a potential method for cancer therapy.

关键词： Photothermal therapy Paclitaxel Combination therapy Gold nanorods Albumin Tumor-associated macrophage

A novel CRISPR/Cas9 system for efficiently generating Cas9- free multiplex mutants in Arabidopsis

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aBIOTECH 2020年第1期1卷 6-14页

作者： Jiajun Wang Haodong Chen State Key Laboratory of protein and Plant Gene Research School of Advanced Agricultural Sciences and School of Life SciencesPeking-Tsinghua Center for Life SciencesPeking UniversityBeijing 100871China

The CRISPR/Cas9 genome-editing system has emerged as a popular powerful tool for biological ***,the process of selecting efficiently edited Cas9-free plants is usually laborious and time ***,we demonstrated P2A to be the most efficient self-cleaving peptide for fusing Cas9 and GFP in Arabidopsis and then used Cas9-P2A-GFP to develop a novel CRISPR/Cas9 ***,a pair of isocaudomer restriction enzymes were selected to conveniently assemble multiple *** this system,the GFP fluorescence intensity in T1 transgenic plants indicates the expression level of the Cas9 protein,which correlates well with the editing ***,Cas9-free plants can be easily selected by examining GFP fluorescence in T2 transgenic *** efficient knockout of BRI1,BZR1 and BES1 demonstrated the robustness of our new ***,we designed a novel CRISPR/Cas9 system that can generate Cas9-free multiplex mutants efficiently in Arabidopsis and possibly in other plant species.

关键词： CRISPR Cas9 Cas9-free P2A Multiplex mutant Arabidopsis

4.4 A Resolution Cryo-EM structure of human mTOR Complex 1

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protein & Cell 2016年第12期7卷 878-887页

作者： Huirong Yang Jia Wang Mengjie Liu Xizi Chen Min Huang Dan Tan Meng-Qiu Dong Catherine C. L, Wong Jiawei Wang Yanhui Xu Hong-Wei Wang Fudan University Shanghai Cancer center Institute of Biomedical Sciences Shanghai Medical College of Fudan University Shanghai 200032 China Key Laboratory of Molecular Medicine Ministry of Education Department of Systems Biology for Medicine School of Basic Medical Sciences Shanghai Medical College of Fudan University Shanghai 200032 China State Key Laboratory of Genetic Engineering Collaborative Innovation Center of Genetics and Development School of Life Sciences Fudan University Shanghai 200433 China Ministry of Education Key Laboratory of protein Sciences Tsinghua-Peking Joint Center for Life Sciences Beijing Advanced Innovation Center for Structural Biology School of Life Sciences Tsinghua University Beijing 100084 China National center for protein Science Shanghai Institute of Biochemistry and Cell Biology Shanghai Institutes of Biological Sciences Chinese Academy of Sciences Shanghai 200031 China National Institute of Biological Sciences Beijing 102206 China

Mechanistic target of rapamycin （mTOR） complex 1 （mTORC1） integrates signals from growth factors, cel- lular energy levels, stress and amino acids to control cell growth and proliferation through regulating trans- lation, autophagy and metabolism. Here we determined the cryo-electron microscopy structure of human mTORC1 at 4.4 A resolution. The mTORCI comprises a dimer of heterotrimer （mTOR-Raptor-mLST8） mediated by the mTOR protein. The complex adopts a hollow rhomboid shape with 2-fold symmetry. Notably, mTORC1 shows intrinsic conformational dynamics. Within the complex, the conserved N-terminal caspase- like domain of Raptor faces toward the catalytic cavity of the kinase domain of mTOR. Raptor shows no caspase activity and therefore may bind to TOS motif for sub- strate recognition. Structural analysis indicates that FKBP12-Rapamycin may generate steric hindrance forsubstrate entry to the catalytic cavity of mTORCI. The structure provides a basis to understand the assembly of mTORC1 and a framework to characterize the regu- latory mechanism of mTORC1 pathway.

关键词： mTORC1 structure cryo-electronmicroscopy

Natural variation of H3K27me3 modification in two Arabidopsis accessions and their hybrid

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Journal of Integrative Plant Biology 2016年第5期58卷 466-474页

作者： Mei Yang Xuncheng Wang Hao Huang Diqiu Ren Yan'e Su Pan Zhu Danmeng Zhu Liumin Fan Liangbi Chen Guangming He Xing Wang Deng State Key Laboratory of protein and Plant Gene Research Peking-Tsinghua Center for Life Sciences School of Advanced Agricultural Sciences and School of Life Sciences Peking University Tsinghua-Peking Joint center for Life Sciences School of Life SciencesTsinghua University Department of Botany College of Life Sciences Hunan Normal University

Histone modifications affect gene expression, but the mechanism and biological consequence of natural variation in histone modifications remain unclear. Here, we generated genome-wide integrated maps of H3K27me3 modification and transcriptome for Col, C24 and their F1 hybrid. A total of 1,828 genomic regions showing variation in H3K27me3 modification between Col and C24 were identified, most of which were associated with genic regions. Natural variation of H3K27me3 modification between parents could result in aUelic bias of H3K27me3 in hybrids. Furthermore, we found that H3K27me3 variation between Col and C24 was negatively correlated with gene expression differences between two accessions, espe- cially with those arising from the cis-effect. Importantly, mutation of CLF, an Arabidopsis methyltransferase for H3K27,altered gene expression patterns between the parents. Together, these data provide insights into natural variation of histone modifications and their association with gene expression differences between Arabidopsis ecotypes.

关键词： Allelic bias Arabidopsis gene expression H3K27me3 natural variation