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Augmenter of liver regeneration promotes hepatocyte proliferation induced by Kupffer cells

World Journal of Gastroenterology 2006年第30期12卷 4859-4865页

作者： Chun-Ping Wang Lin Zhou Shu-Hui Su Yan Chen Yin-Ying Lu Fei Wang Hong-Jun Jia Yong-Yi Feng Yong-Ping Yang Department of Gastroenterology 302 Hospital of Chinese PLA Beijing 100039 China

AIM： To observe the effects of augmenter of liver regeneration （ALR） on Kupffer cells and to determine whether ALR promotes hepatocyte proliferation induced by Kupffer cells. METHODS： Kupffer cells and hepatocytes were cultured in vitro and various concentrations of recombinant rat ALR （rrALR） were added. ^3H-thymidine, BrdU and ^3H-leucine incorporation was determined in cultured Kupffer cells and hepatocytes, in hepatocytes conditioned by Kupffer cells, and in associated medium, rrALR was labeled by iodination and used to determine its binding activity by Scatchard analysis in Kupffer cells and primarily cultured rat hepatocytes. RESULTS： rrALR stimulated DNA replication in Kupffer cells and protein synthesis both in cells and in medium in a no.-concentration-dependent manner. The effect was significant at the concentration of 1μg/L ALR. However, rrALR had no.effect on primarily cultured hepatocytes, when hepatocytes were cultured with the Kupffer cell medium conditioned by ALR, DNA replication and protein synthesis in hepatocytes increased significantly at the concentration of 1μg/L ALR. When the ALR concentration was increased, its effect on hepatocyte proliferation decreased to the basal level. Scatchard analysis indicated the presence of a single class of high affinity receptors with a dissociation constant （Kd） of 0.883 nmol/L and a maximum binding capacity （Bmax） of 126.1 pmol/g protein in the rat Kupffer cells. CONCLUSION： ALR can promote hepatocyte proliferation induced by Kupffer cells, which is associated with the concentration of ALR, suggesting that Kupffer cells play a dual role in liver regeneration.

关键词： Liver regeneration Augmenter of liver regeneration Kupffer cell

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