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A simple taurocholate-induced model of severe acute pancreatitis in rats

World Journal of Gastroenterology 2009年第45期15卷 5732-5739页

作者： Zhong-Hui Liu Jun-Sheng Peng Chu-Jun Li Zu-Li Yang Jun Xiang Hu Song Xiao-Bing Wu Jun-Rong Chen De-Chang Diao Department of Gastrointestinal Surgery the Sixth Affiliated Hospital of Sun Yat-sen University 26 Yuanchun Erheng Road Guangzhou 510655 Guangdong Province China Department of Gastroenterology the Sixth Affiliated Hospital (Gastrointestinal & Anal Hospital) of Sun Yat-sen University 26 Yuanchun Erheng Road Guangzhou 510655 Guangdong Province China

AIM: To investigate gut barrier damage and intestinal bacteria translocation in severe acute pancreatitis (SAP), a simple rat model of SAP was induced and ***: Pancreatitis was induced by uniformly distributed injection of 3.8% Na taurocholate (1 mL/kg) beneath the pancreatic capsule. Rats in the control group were injected with normal saline in the identical location. RESULTS: Serum amylase, plasma endotoxin, intestinal permeability, and pancreatitis pathology scores were all markedly higher in the pancreatitis group than in the control group (P < 0.01). The bacterial infection rate was signif icantly higher in the SAP group than in the control group (P < 0.01), observed in parallel by both bacterial culture and real-time polymerase chain reaction. Acute damage of the pancreas was observed histologically in SAP rats, showing interstitial edema, leukocyte infiltration, acinar cell necrosis and hemorrhage. The microstructure of the intestinal mucosa of SAP ratsappeared to be destroyed with loose, shortened microvilli and rupture of the intercellular junction, as shown by electron microscopy. CONCLUSION: Significant gut barrier damage and intestinal bacterial translocation were def initely observed with few potential study confounders in this SAP rat model, suggesting that it may be an appropriate animal model for study of gut barrier damage and bacterial translocation in SAP.

关键词： Acute pancreatitis Bacterial translocation Inflammation Real-time polymerase chain reaction

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