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EFFECTS OF TEMPERATURE AND NUTRITION ON THE GERMINATION OF VERTICILLIUMLECANII CONIDIA

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EFFECTS OF TEMPERATURE AND NUTRITION ON THE GERMINATION OF V...

The ⅩⅤth International Plant Protection Congress

作者： YU Rong-rong and ZHANG Long Key Lab of Ministry of Agriculture for biological control of Pests china Agricultural University beijing 10009 china

非OI群霍乱弧菌在不同理化环境中产生色素的研究

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微生物学通报 1996年第4期23卷 220-221页

作者：黄廷学黄春德陈天寿海口卫生检疫局中国药品生物制品检定所

本文报道了理化因素对非ＯＩ群霍乱弧菌的生长及产生色素的影响。实验结果表明，２５℃～３６℃、０％－３％ＮａＣｌ、ｐＨ７．４～９．０；４２℃、１％～３％ＮａＣｌ、ｐＨ５．０—１０．０为最佳生长环境，适宜生长，并有利于色素... 详细信息

本文报道了理化因素对非ＯＩ群霍乱弧菌的生长及产生色素的影响。实验结果表明，２５℃～３６℃、０％－３％ＮａＣｌ、ｐＨ７．４～９．０；４２℃、１％～３％ＮａＣｌ、ｐＨ５．０—１０．０为最佳生长环境，适宜生长，并有利于色素的产生。

关键词：非OI群霍乱弧菌色素理化因素环境

Microsatellite Analysis in 2 Populations of Kunming Mice

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Microsatellite Analysis in 2 Populations of Kunming Mice

实验动物与药理学、毒理学研究学术交流会

作者： Haitao Shang~1,Hong Wei~1,Bingfei Yue~2,Ping Xu~3,Honggang Huang~4 1Department of Laboratory Animal Science,College of Basic Medicine,Third Military Medical University, Chongqing 400038,People's Republic of china 2 National institute for the control of Pharmaceutical and biological products,beijing 100050,People's Republic of china 3Shanghai Laboratory Animal Science Center,Chinese Academy of Sciences,Shanghai 201615,People's Republic of china 4institute of Animal Science,Chinese Academy of Agricultural Sciences,beijing 100094,People's Republic of china

Objective To evaluate the molecular genetic profiles of Kunming mice and the extent of genetic differentiation among *** 15 microsatellite markers were screened by a fluorescence-based semi-automated genotyping method for the 2 main populations of Kunming mice from beijing(BJ) and Shanghai(SH) in *** observed number of alleles,effective number of alleles,observed heterozygosity,unbiased expected heterozygosity and Shannon information index were used to estimate the genetic variation within *** A total of 89 alleles were detected in the 2 populations,with 2-12 at each locus,and the mean unbiased expected heterozygosity was 0.5724,which implies that there is abundant genetic variation in the populations of Kunming *** differentiation was shown by shared alleles,F-statistics,Nei genetic distance and Nei genetic *** population BJ and population SH,respectively,only 35 of 61 and 35 of 63 alleles were shared by *** Fst per locus varied from 0.0131(D2Mit30) to 0.5697(D7Mit281) and the average Fst of all loci was 0.1433,which indicates moderate genetic differentiation between the 2 Kunming mice *** differences were also observed by Nei's(1972) genetic distance(0.3987)and Nei's(1978) unbiased measures of genetic distance(0.3881) estimates of *** There is abundant genetic variation in the populations of Kunming mice,and moderate genetic differentiation between the 2 Kunming mice populations.

关键词： Kunming mice Microsatellite Outbred colony Genetic differentiation

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昆虫病原线虫共生菌的分类

微生物学报 2003年第4期43卷 527-533页

作者：庞在堂杨怀文中国农业科学院生物防治研究所北京100081

昆虫病原线虫共生菌是存在于昆虫病原线虫肠道内的一类细菌,属肠杆菌科(Enterobacteriaceae),兼性厌氧,包括致病杆菌属(Xenorhabdus)[1]和光杆状菌属(Photorhabdus)[2],它们分别与斯氏线虫属(Steinernema)和异小杆线虫属(Heterorhabditis)的线虫共生[3].在自然界,共生菌存在于3龄侵染期线虫的肠道中[4],不能从土壤中分离获得,是昆虫病原线虫的主要致病因子.

关键词：昆虫病原线虫共生菌分类致病杆菌属光杆状菌属

Molecular biology evaluation of Polyacrylamide Hydrogel using real time PCR

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Molecular biology evaluation of Polyacrylamide Hydrogel usin...

第2届世界华人生物医学工程研讨会

作者： Chengxiang Fan1 Tingfei Xi1 a Ziyi Wan2 Xixiong Kang3 Yajie Wang3 Chunren Wang1 1. Center for Medical Devices National institute for the control of Pharmaceutical & biological products beijing 100050 P.R.china 2. School of Materials Science and Engineering beijing institute of Technology beijing 100081 P.R.china 3. Titan Hospital Capital University of Medical Science beijing 100050 P.R.china a

Polyacrylamide Hydrogel, which is widely used in ophthalmic operations, drug treatment, food packaging products, and water purification etc, is a watery gel consisting of approximately 2.5% cross-lin

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Screening of Proteins Interacting with Nonstructural 1 Protein of H5N1 Avian Influenza Virus from T7-phage Display Library

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Chemical Research in Chinese Universities 2012年第1期28卷 103-107页

作者： ZHU Chun-yu1,2, SUN Ting-ting2, ZHAO Jian1,2, WANG Ning1,2, ZHENG Fang-liang1, AI Hai-xin1, ZHU Jun-feng1,2, WANG Xiao-ying3, ZHU Ying4, WU Jian-guo4 and LIU Hong-sheng1,2 1. Key Laboratory of Animal Resource and Epidemic Disease Prevention of Liaoning Province, 2. Research Center for Computer Simulating and Information Processing of Bio-macromolecules of Liaoning Province, Academy of Science, Liaoning University, Shenyang 110036, P. R. china 3. institute of Nutrition and Food Safety, Chinese Center for Disease control and Prevention, beijing 100050, P. R. china 4. State Key Laboratory of Virology, Wuhan University, Wuhan 430072, P. R. china Research Center for Computer Simulating and Information Processing of Bio-macromolecules of Liaoning Province Academy of Science Liaoning University Key Laboratory of Animal Resource and Epidemic Disease Prevention of Liaoning Province Shenyang 110036 Research Center for Computer Simulating and Information Processing of Bio-macromolecules of Liaoning Province Academy of Science Liaoning University Shenyang 110036 Liaoning University Key Laboratory of Animal Resource and Epidemic Disease Prevention of Liaoning Province Shenyang 110036 institute of Nutrition and Food Safety Chinese Center for Disease Control and Prevention Beijing 100050 Wuhan University State Key Laboratory of Virology Wuhan 430072

Avian influenza virus（AIV） nonstructural 1（NS1） gene was amplified by real-time polymerse chain reac tion（RT-PCR） and inserted into pET28a, then transformed into E. coli BL21（DE3） competent cell. With the induction of isopropyl-β-D-thiogalactoside（IPTG） and the purification of Ni-NTA column, we finally obtained purified NS1 protein. T7-phage display system was used to screen the proteins that interacted with NS1 from lung cell cDNA li brary. The selected positive clones were identified by DNA sequencing and analyzed by BLAST program in Gene Bank. Two proteins were obtained as NS1 binding proteins, Homo sapiens nucleolar and coiled-body phosphoprotein 1（NOLC1） and Homo sapiens similar to colon cancer-associated antigen. By co-immunoprecipitation and other me thods, Homo sapiens NOLC1 was found to interact with the NS1 protein, the results would provide the basis for fur ther studying biological function of NS1 protein.

关键词： T7-phage display Nonstructural 1 (NS 1) protein Interacting protein

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一个新的钩端螺旋体血清群——曼耗群

微生物学报 1979年第3期 230-234页

作者： institute of Military, Department of Logistics of Kunming Military Area(Kunming)National institute for the control of Pharmaceutical biological products, Ministry of Health(beijing) 昆明军区后勤部军事医学研究所卫生部药品生物制品检定所

本文介绍了致病性钩端螺旋体的一个新的血清群——曼耗(Manhao)群的分群结果。曼耗群钩端螺旋体与Javanica,Celledoni Canicola, Cynopteri,Autumnalis, Australis,Po-mona,Grippotyphosa,Hebdomadis,Bataviae,Tarassovi,Shermam,Panama等群各型没有阳性交叉反应。仅与Pyrogenes群中的alexi型HS 616株有共同的抗原因子联系,与Pyrogenes群内pyrogenea型等其他型没有交叉反应;另外还与Icterohaemorrhagiae ,Ballum群个别型有低度的,不稳定的交叉反应。因此确定曼耗群是钩端螺旋体的一个新血清群。到目前为止,本群各型地方株中除一株是由猪肾分离的外,其余菌株均获自钩端螺旋体病患者。尚未从其它常见的宿主动物中获得过本群钩端螺旋体。

关键词：血清群国际参考血清型血清变种钩端螺旋体标准菌株

A membrane-tethered transcription factor ANAC089 negatively regulates floral initiation in Arabidopsis thaliana

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Science china(Life Sciences) 2010年第11期53卷 1299-1306页

作者： LI JianQin1,2, ZHANG Juan1, WANG XueChen1 & CHEN Jia1 1 State Key Laboratory of Plant Physiology and Biochemistry,College of biological Sciences, china Agricultural University, beijing 100193, china 2 College of Life Science and Technology, Guangxi University, Nanning 530005, china State Key Laboratory of Plant Physiology and Biochemistry College of Biological Sciences China Agricultural University Beijing China College of Life Science and Technology Guangxi University Nanning China

The plant-specific NAC (NAM, ATAF1/2,and CUC2) transcription factors have a regulatory function in developmental processes and stress responses. Notably a group of NAC members named NTLs (NTM1-Like) are membrane-tethered, ensuring plants rapidly respond to developmental changes and environmental stimuli. Our results indicated that ANAC089 was a membrane-tethered transcription factor and its truncated form was responsible for the physiological function in flowering time control.

关键词： Arabidopsis thaliana ANAC089 floral initiation membrane-tethered transcription factor

The impact of vegetation restoration on erosion-induced sediment yield in the middle Yellow River and management prospect

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Science china Earth Sciences 2005年第6期48卷 724-741页

作者： CHEN Hao1, ZHOU Jinxing2, CAI Qiangguo1, YUE Zhongqi3, LU Zhongchen4, LIANG Guanglin1& HUANG Jianguo5 1. Key Laboratory of Water Cycle and Related Land Surface Processes, institute of Geographic Sciences and Natural Resources Re-search, Chinese Academy of Sciences, beijing 100101, china 2. institute of Forestry, Chinese Academy of Forestry Sciences, beijing 100091, china 3. Department of Civil Engineering, University of Hong Kong, Hong Kong, china 4. Eco-environmental Research Center, Chinese Academy of Sciences, beijing 100085, china 5. College of Resources and Environment, Southwest Agricultural University, Chongqing 400716, china Correspondence should be addressed to Chen Hao Key Laboratory of Water Cycle and Related Land Surface Processes Institute of Geographic Sciences and Natural Resources Research Chinese Academy of Sciences Beijing China institute of Forestry Chinese Academy of Forestry Sciences Beijing China Department of Civil Engineering University of Hong Kong Hong Kong China Eco-environmental Research Center Chinese Academy of Sciences Beijing China College of Resources and Environment Southwest Agricultural University Chongqing China

According to the characteristics of water and erosion environments of different natural zones on the Loess Plateau, this paper studies changes of vegetation types, distribution boundaries of forest and grass as well as restoration capacity of vegetation in different natural zones in the middle Yellow River. The annual precipitation of 530 mm is the critical annual pre-cipitation for forest and grass distribution in the middle Yellow River. Among the zonal and azonal environmental factors affecting watershed sediment yield, the intermediate diameter D50 (mm) of suspended load and forest coverage (V, %) play the leading role. Of them the effect weight of forest coverage (V, %) on catchments sediment yield is only 3.4% less than the role of the intermediate diameter (D50, mm), they are almost the same. To effectively control soil erosion in semiarid, especially in hilly-gullied areas and make sediment transport modulus reduce to less than 6000 t/km2, it is rather difficult by merely relying on natural restoration of forest. In the process of cultivated land conversion into forest land and grassland, measures suiting local conditions should be adopted in tree species selection and artificial afforestation (grass planting) based on management with biological measures for slopeland and engineering measures for hilly-gullied areas, so that watershed forest coverage in key counties can reach at least over 30%.Compared with the standard period of precipitation prior to the 1960s, with the decrease of an-nual precipitation at various periods, plant productivities decline to different degrees under natural conditions. The main reason accountable for the low survival rate of new seedlings and grass over years is due to precipitation decrease. In light with regression models of annual pre-cipitation and natural vegetation productivities, it is possible to obtain estimated values of wa-tershed natural vegetation productivity and eco-water consumption needed for the restoration to

关键词： vegetation restoration, eco-water requirement, watershed sediment yield, the middle Yellow River.