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Effect of Porphyromonas gingivalis PrtC on cytokine expression in ECV304 endothelial cells and its level in subgingival plaques from patients with chronic periodontitis

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Acta Pharmacologica Sinica 2007年第7期28卷 1015-1023页

作者： Yan-min WU~2,Li-li CHEN~(2,6),Jie YAN~(3,6),Chun-yan ZHUANG~4,Zhi-yuan GU~5~2 department of stomatology,the Second affiliated hospital,college of medicine,zhejiang university,hangzhou 310009,china ~3 Depart-ment of Medical Microbiology and Parasitology,college of medicine,zhejiang university,hangzhou 310006,china ~4 department ofstomatology,the affiliated hospital of the School of medicine,Ningbo university,Ningbo 315020,china ~5 department of oral andmaxillofacial surgery,the affiliated hospital of stomatology,college of medicine,zhejiang university,hangzhou 310006,china department of stomatology the Second Affiliated Hospital College ofMedicine Zhejiang University Hangzhou 310009 China. yanchen_5657@***.

Aim:To investigate the effect of the collagenase gene (prtC) product ofPorphyromonas gingivalis on inducing host cells to secrete inflammatorycytokines,and to discuss the correlation between the PrtC level in subgingivalplaque samples and clinical ***:A prokaryotic expression sys-tem pET32a-prtC-Escheria coli BL21 DE3 was *** and im-munoreactivity of the recombinant PrtC protein (rPrtC) was identified by *** was applied to detect interleukin (IL)-1α,IL-8,and TNF-α levelsin supernatants from rPrtC-induced human umbilical vein endothelial cells (HUVEC)originated ECV304 *** parameters recorded at baseline and after treat-ment included bleeding on probing (BOP),probing depth (PD),and attachmentloss (AL).ELISA was established to measure the PrtC level in 196 subgingivalplaque samples from 49 patients with chronic ***:After co-incubation with 1 μg/mL rPrtC for 24 h and with 5 or 10 μg/mL rPrtC for 12 h,thelevels of IL-1α,IL-8,and TNF-α secreted by the ECV304 cells increased signifi-candy (P5).The PrtC level in the BOP-positive or the ≥5 mm AL or>6 mm PDsites was higher than that in the BOP-negative or the ≤2 mm AL or ≤6 mm PD sites(P5),*** with baseline,the PrtC levels in different ALsites or in the ≤6 mm PD pockets decreased remarkably after treatment (P6 mm PD sites,the PrtC levels changed insignifi-cantly (P>0.05).Conclusion:rPrtC is able to directly induce host cells to synthe-size and secrete IL-1α,IL-8,and TNF-α.The PrtC level in subgingival samples iscorrelated with BOP,AL,and PD.

关键词： Porphyromonas gingivalis collagenase cloning cytokine ELISA

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Bridging properties of the debonded interface with frictional sliding

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Progress in Natural Science:Materials International 2006年第2期16卷 153-162页

作者： LIU Pengfei, TAO Weiming, YU Xuezhong and GUO Yimu ( department of Engineering Mechanics, zhejiang university, hangzhou 310027, china Second affiliated hospital, zhejiang university, hangzhou 310009, china) [a]department of Engineering Mechanics Zhejiang University Hangzhou China [b]Second affiliated hospital Zhejiang University Hangzhou China

A theoretical model simulating the properties of frictional bridging is presented and relatively perfect stress solutions for the fiber and matrix are obtained. Based on the energy equilibrium in the process of interfacial debonding, an expression for the energy release rate G is derived to explore the interfacial fracture properties. By introducing an interfacial debonding criterion G≥Γi, a method for determining the critical debond length is proposed and the bridging constitutive relationship is also obtained. Numerical calculations are conducted for the fiber-reinforced composite SCS-6/Ti-6Al-4V and the results are also compared with those obtained by using other existing models.

关键词： interfacial stress transfer frictional sliding energy release rate bridging constitutive relationship fiber-reinforced composites.

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Progress in research into the mechanism of Radix salviae miltiorrhizae in treatment of acute pancreatitis

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Hepatobiliary & Pancreatic Diseases International 2006年第4期5卷 501-504页

作者： Xi-Ping Zhang, Zhi-Jun Li and Da-Ren Liu department of General surgery, hangzhou First People’s hospital, hangzhou 310006, china , zhejiang university of Traditional Chinese Medine, hangzhou 310053, china department of General surgery, Second affiliated hospital, zhejiang university School of medicine, hangzhou 310003, china department of General surgery Hangzhou First People's Hospital Hangzhou 310006 China. zxp99688@***

BACKGROUND: Acute pancreatitis (AP) is a common acute abdomen with high mortality, multiple complications and complicated causes. There is no effective therapy for AP. Radix salviae miltiorrhizae (Danshen), a traditional herbal medicine, has a low price and a wide range of clinical applications. It is effective to promote blood flow, eliminate stagnancy, and relieve pain. It is also found to be effective in treating AP. We reviewed the progress in research into the mechanism of Radix salviae miltiorrhizae in treatment of AP. DATA SOURCES: An english-language literature search was conducted using MEDLINE (1988-2005) on Radix salviae miltiorrhizae (Danshen) and acute pancreatitis. RESULTS: The mechanisms of Danshen in the treatment of AP include improvement of microcirculatory disturbances; elimination of oxygen free radicals; modulation of the metabolism of lipid inflammatory mediator; and blocking of calcium inflow and prevention of calcium ***: Danshen can effectively reduce the mortality and complications of AP.

关键词： Radix salviae miltiorrhizae (Danshen) acute pancreatitis mechanism

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Changes of phospholipase D activity of rat peritoneal mast cells in degranulation

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Acta Pharmacologica Sinica 2004年第1期 106-111页

作者： Yun- bi LU, Ming WU, Han-liang ZHOUdepartment of Pharmacology, School of medicine, zhejiang university, hangzhou 310031 department of Cardiovascular Thoracic surgery, Second affiliated hospital, School of medicine, zhejiang university, hangzhou 310009, china

IM: To study the changes of phospholipase D (PLD) activity of actively sensitized rat peritoneal mast cells (RPMC) in degranulation. METHODS: Degranulation of RPMC was determined by measurement of β-hexosaminidase release. PLD activity assay was carried out by measurement of PLD product, choline, with chemiluminescent oxidation of luminol. RESULTS: Actively sensitized RPMC challenged with ovalbumin (0.5-8 mg/L for 120 s, 4 mg/L for 15-120 s) resulted in significant activation of PLD accompanied with the increment of P-hexosaminidase release. PLD activity of sensitized RPMC was increased by more than 2-fold compared with that of unsensitized RPMC which contained low levels of PLD activity [(35±13) pmol choline/min in 1×106cells], but β-hexosaminidase releases of the sensitized cells were as low as spontaneous releases.

关键词： ovalbumin phospholipase D immunization mast cells

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Association of apolipoprotein E 4 polymorphism with cerebral infarction in Chinese Han population

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Acta Pharmacologica Sinica 2004年第03期25卷 98-102页

作者： Zhu-qing JIN2,Yong-sheng FAN3,Jing DING4,Mei CHEN5,Wei FAN4,Guang-ji ZHANG2,Bin-hui ZHANG5,Suo-jing YU2,Yong-sheng ZHANG2,Wei-feng JI5,Jian-gang ZHANG6 2Genomic Research Centre,zhejiang college of Traditional Chinese medicine,hangzhou 310053 3Schoolof Basic Medical Sciences,zhejiang college of Traditional Chinese medicine,hangzhou 310053 4department of Neurology,Zhongshan hospital,Fudan university,Shanghai200032 5department of Neurology,zhejiang hospital of Traditional Chinese medicine,zhejiang collegeof Traditional Chinese medicine,hangzhou 310006,china Genomic Research Centre Zhejiang College of Traditional Chinese Medicine Hangzhou 310053 School of Basic Medical Sciences Zhejiang College of Traditional Chinese Medicine Hangzhou 310053 department of Neurology Zhongshan Hospital Fudan University

AIM: To study the association between APOE polymorphisms and cerebral infarction through a case-control study among the Chinese Han population. METHODS: First-ever cerebral infarction patients (n=226) whose ages ranged from 40 to 60 years old were recruited from department of Neurology, Zhongshan hospital, Shanghai, and zhejiang Chinese Traditional medicine hospital, zhejiang, china. Unrelated healthy controls (n=201) were selected from the general population in the same area with similar age and sex distribution. APOE was amplified by one-stage PCR using the forward primer: 5'-GGC ACG GCT GTC CAA GGA GCT-3' and reverse primer: 5'-GAT GGC GCTGAG GCC GCG CT-3'. The PCR product was digested directly with 5 U of CfoI and separated by a 20 % polyacryla- mide (acrylamide: bis-acrylamide=29:1) nondenaturing gel. RESULTS: Both cerebral infarction patient and control groups were in Hardy-Weinberg equilibrium. The allele frequency of APOE*2, APOE*3, and APOE*4 was 4.6 %, 81.9 %, and 13.5 % respectively in the patients with cerebral infarction; 5.7 %, 87.3 %, and 7.0 % respectively in the healthy control group. Compared with APOE3/3 subjects, APOE4/4 carriers had a 2.1-fold risk of cerebral infarction (odds ratio 2.1, 95 % confidence limits 1.3 to 3.4). The allele frequency of APOE*4 in the cerebral infarction patient group was significantly higher than that in the control group (13.5 % vs 7.0 %; P=0.002). CONCLUSION: APOE 4 is a risk factor for cerebral infarction among the Chinese Han population.

关键词： apolipoproteins E human alleles polymorphism brain infarction

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Kupffer cells contribute to concanavalin A-induced hepatic injury through a Th1 but not Th17 type response-dependent pathway in mice

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Hepatobiliary & Pancreatic Diseases International 2011年第2期10卷 171-178页

作者： Lin Chen,Xiao-Jun Xie,Yu-Fu Ye,Lin Zhou,Hai-Yang Xie,Qin-Fen Xie, Jiong Tian and Shu-Sen Zhengzhejiang university School of medicine,hangzhou 310003,chinaKey Laboratory of Combined Multi-organ Transplantation,Ministry of Public Health department of Hepatobiliary and Pancreatic surgery department of Nephrology,First affiliated hospital,zhejiang university School of medicine,hangzhou 310003,china [a] zhejiang university School of medicine Hangzhou 310003 China [b] Key Laboratory of Combined Multi-organ Transplantation Ministry of Public Health department of Hepatobiliary and Pancreatic surgery First Affiliated Hospital Zhejiang University School of Medicine Hangzhou 310003 China [c] department of Nephrology First Affiliated Hospital Zhejiang University School of Medicine Hangzhou 310003 China

BACKGROUND:Increasing evidence suggests that a close interaction of Kupffer cells with T cells plays a central role in concanavalin A-induced hepatic injury in mice,but the underlying mechanisms remain *** present study aimed to determine the relative roles of Th1 and Th17 type responses in concanavalin A-induced hepatic injury in mice, and to investigate whether or not Kupffer cells contribute to hepatic injury via a Th1 or Th17 type response-dependent pathway. METHODS:Immune-mediated hepatic injury was induced in C57BL/6 mice by intravenous injection of concanavalin A. Kupffer cells were inactivated by pretreatment with gadolinium chloride 24 hours before the concanavalin A *** interferon-gamma(IFN-γ)and interleukin-17(IL-17)pathways were blocked by specific neutralizing *** injury was assessed using serum transferase activity and pathological *** of inflammatory cytokines within the liver was detected by real-time polymerase chain reaction and immunohistochemistry. RESULTS:Neutralization of IFN-γsignificantly attenuated concanavalin A-induced hepatic ***,neutralization of IL-17 failed to suppress the *** of Kupffer cells by gadolinium chloride pretreatment protected against concanavalin A-induced injury and significantly reduced hepatic cytokine levels including TNF-α,IL-6 and IFN-γbut not ***:Our findings suggest that Kupffer cells contribute to concanavalin A-induced hepatic injury via a Th1 type response-dependent pathway and production of inflammatory cytokines including TNF-α,IL-6 and IFN-γ.

关键词： Kupffer cells interferon-gamma interleukin-17 concanavalin A hepatic injury hepatitis

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Liver transplantation in china: problems and their solutions

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Hepatobiliary & Pancreatic Diseases International 2004年第2期3卷 170-174页

作者： Jian Wu and Shu-Sen Zheng hangzhou, china department of Hepatobiliary surgery, First affiliated hospital, zhejiang university School of medicine, hangzhou 310003 , Chi- na department of Hepatobiliary surgery First Affiliated Hospital Zhejiang University School of Medicine Hangzhou 310003 China.

BACKGROUND: The past decade has witnessed the rapid development of liver transplantation in china. The 1-year survival of liver transplant patients comes to 80% in many leading medical centers and the number of liver transplanta- tion is increasing. However, liver transplantation in china is facing several challenges including recipient with hepato- cellular carcinoma (HCC), recurrence of HCC and hepati- tis B, long-term postoperative care, the bridge to liver transplantation, and shortage of liver donor. This review was to understand the status of and problems in liver trans- plantation in china. DATA RESOURCES: An English-language literature search using MEDLINE (1990-2003) on liver transplantation and other related reports and review articles in Chinese from major transplant centers in china. RESULTS: HCC is one of the main indications for liver transplantation in china but different centers adopted dif- ferent criteria for selection of patients. Hepatitis B virus re- infection is a vital problem after liver transplantation in HBV-related patients. More and more attention was fo- cused on long-term postoperative care and donor shortage. Artificial liver support system has been applied in patients waiting for a graft in many centers. CONCLUSIONS: HCC remains to be one of the main indi- cations for liver transplantation in china; combined hepati- tis B immune globulin and lamivudine is considered effec- tive to prevent hepatitis B virus reinfection. Apart from long-term postoperative care for the improvement of the survival rate, early steroid withdrawal is feasible in liver transplantation. Living donor liver transplantation, split liv- er transplantation, and marginal donor transplantation can deal with donor shortage to some extent. Artificial liver as- sist system serves as a bridge to liver transplantation.

关键词： liver transplantation hepatocellular carcinoma hepatitis B virus donor shortage artificial liver support system, postoperative care

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Resting state brain activity and functional brain mapping

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Progress in Natural Science:Materials International 2007年第5期17卷 511-519页

作者： Zhao Xiaohu , Wang Peijun and Tang Xiaowei (college of life Sciences in zhejiang university, hangzhou 310058, china Imaging department of Tong Ji hospital of Tong Ji university, Shanghai 200065, china Bio-X Lab, department of Physics, zhejiang university, hangzhou 310027, china) [a]college of life Sciences in zhejiang university Hangzhou China [b]Imaging department of Tong Ji hospital of Tong Ji university Shanghai China [c]Bio-X Lab Department of Physics Zhejiang University Hangzhou China

Functional brain imaging studies commonly use either resting or passive task states as their control conditions, and typically identify the activation brain region associated with a specific task by subtracting the resting from the active task conditions. Numerous studies now suggest, however, that the resting state may not reflect true mental "rest" conditions. The mental activity that occurs during "rest" might therefore greatly influence the functional neuroimaging observations that are collected through the usual subtracting analysis strategies. Exploring the ongoing mental processes that occur during resting conditions is thus of particular importance for deciphering functional brain mapping results and obtaining a more comprehensive understanding of human brain functions. In this review article, we will mainly focus on the discussion of the current research background of functional brain mapping at resting state and the physiological significance of the available neuroimaging data.

关键词： resting state brain activity default mode brain networks BOLD deactivation OEF.

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Inhibitory effect of cyclosporine A on hepatitis B virus replication in vitro and its possible mechanisms

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Hepatobiliary & Pancreatic Diseases International 2005年第1期4卷 18-22页

作者： Wei-Liang Xia, Yan Shen and Shu-Sen Zheng hangzhou, china department of Hepatobiliary surgery, First affiliated hospital, zhejiang university School of medicine, hangzhou 310003, china department of Hepatobiliary surgery First Affiliated Hospital Zhejiang University School of Medicine Hangzhou 310003 China.

BACKGROUND: Hepatitis B related end-stage liver disease is recently acknowledged as one of the main indications for orthotopic liver transplantation (OLT). However, the high recurrence rate of hepatitis B virus infection following transplantation is regarded as a major factor affecting the long-term survival of transplant recipients especially in Chi- na. Cyclosporine A (CsA), which is routinely used to pre- vent the allograft rejection, is reported to have the inhibito- ry activity on hepatitis B virus (HBV) replication in vitro. In this paper, we review the inhibitory effect and its possi- ble mechanisms of CsA on HBV replication in vitro. DATA RESOURCES: An English-language literature search was conducted using MEDLINE (1990-2004) on cyclospo- rine A, hepatitis B virus, mitochondria, calcium and other related reports and review articles. RESULTS: Hepatitis B x protein (HBx) is essential to HBV replication. The cytosolic calcium signaling mediated by mitochondria and the Src kinase pathway were involved during HBx activation of HBV replication. CsA inhibits the HBV replication in vitro by its binding to mitochondrial cy- clophilin D, then blocking the mitochondria-mediated cy- tosolic calcium signaling. The derivates of CsA also have the HBV replication inhibitory effect in vitro. CONCLUSIONS; By interacting with mitochondria, pre- venting the release of intramitochondrial calcium, and then blocking the cytosolic calcium signaling, CsA inhibits the HBV replication in vitro. The derivates of CsA also have this activity.

关键词： hepatitis B virus cyclosporine A calcium

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Liver transplantation in Crigler-Najjar syndrome type I disease

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Hepatobiliary & Pancreatic Diseases International 2012年第5期11卷 545-548页

作者： Zhen-Hua Tu, De-Sheng Shang, Jin-Cai Jiang, Wu Zhang, Min Zhang, Wei-Lin Wang, Hai-Yan Lou,Shu-Sen Zheng Division of Hepatobiliary and Pancreatic surgery, department of surgery, First affiliated hospital, zhejiang university School of medicine, Key Laboratory of Combined Multi-Organ Transplantation, Ministry of Public Health & Key Laboratory of Organ Transplantation of zhejiang Province department of Radiology,First affiliated hospital,zhejiang university School of medicine, hangzhou 310003, china [a] Division of Hepatobiliary and Pancreatic surgery Department of Surgery First Affiliated Hospital Zhejiang University School of Medicine Key Laboratory of Combined Multi-Organ Transplantation Ministry of Public Health & Key Laboratory of Organ Transplantation of Zhejiang Province Hangzhou 310003 Hangzhou China [b] department of Radiology First Affiliated Hospital Zhejiang University School of Medicine Hangzhou 310003 Hangzhou China

BACKGROUND: Crigler-Najjar syndrome type I (CNS I) is a very rare autosomal recessive inherited disease that liver transplantation can properly deal with. METHODS: We present one case of an 18-month-old child with CNS I diagnosed by clinical findings and genetic detecting LTx was performed 5 days after kernicterus broke out and neurological symptoms were successfully reversed. RESULT: Magnetic resonance imaging and magnetic resonance spectroscopy showed encouraging results that brain pathology had a trend to return to normal in 1-year follow-up, combined with electroencephalogram and motor development estimate studies. CONCLUSIONS: Liver transplantation can cure CNS I with reversible neurological symptoms to some extent in time Magnetic resonance spectroscopy may be a future option of predicting brain conditions and selecting suitable patients with CNS I for transplantation.

关键词： Crigler-Najjar liver transplantation inborn error of metabolism magnetic resonance imaging hyperbilirubinemia

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