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2007中国植物生理学会全国学术会议

作者： Ya-Nan Yang Hong-Wei Xue (National key laboratory of plant molecular genetics,institute of plant physiology & ecology,shanghai institutes for biological Sciences,{12. academy of Sciences,300 Fenglin Road,2.0032 shanghai,P.R.china)

＜正＞Abscisic acid（ABA）is one of the major plant hormones and regulates many aspects of plant growth and development including embryo maturation,seed dormancy,germination, cell division and elongation,and responses to environmental stresses such as drought,salinity,cold,pathogen attack and UV radiation.A rice ABA-insensitive mutant was identified from shanghai T-DNA Insertion Population（SHIP）,and the mutant

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Studies on the regulatory network of Brassinosteroid effects in photomorphogenesis

Studies on the regulatory network of Brassinosteroid effects...

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中国遗传学会植物遗传和基因组学专业委员会2009年学术研讨会

作者： Li Song,Xiao-Yi Zhou,and Hong-Wei Xue~* National key laboratory of plant molecular genetics,institute of plant physiology and ecology,shanghai institutes for biological Sciences,chinese academy of Sciences,300 Fenglin Road,shanghai {12.

Brassinosteroids(BRs) is crucial in regulating plant growth and development,as well as the photomorphogenesis,however,the mechanism,especially how they synergistically function with light and other factors,is still la... 详细信息

关键词： brassinosteroid photomorphogenesis hormone interplay ubquitin

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Comparative Analysis of Two-component Signal Transduction System in Two Streptomycete Genomes

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Acta Biochimica et Biophysica Sinica 2007年第5期39卷 317-325页

作者： WU WEI~(1,3#), Weihua WANG~(2#), Zhiwei CAO~(3#), Hong YU~3, Xiaojing WANG~1, Jing ZHAO~3, Hao TAN~3, Hao XU~3, Weihong JIANG~(2*), and Yixue LI~(1,3*) 1 Bioinformation Center, key Lab of Systems Biology, shanghai institutes for biological Sciences, chinese academy of Sciences, Graduate School of the chinese academy of Sciences, shanghai 200031, china 2 laboratory of molecular Microbiology, {12., shanghai institutes for biological Sciences, chinese academy of Sciences, Graduate School of the chinese academy of Sciences, shanghai 2.0032, china 3 shanghai Center for Bioinformation Technology, shanghai 200235, china BioinformationCenter KeyLabofSystemsBiologyShanghaiInstitutesforBiologicalSciencesChineseAcademyofSciencesGraduateSchooloftheChineseAcademyofSciencesShanghai200031China

Species of the genus Streptomyces are major bacteria responsible for producing most natural antibiotics. Streptomyces coelicolor A3(2) and Streptomyces avermitilis were sequenced in 2002 and 2003, respectively. Two-component signal transduction systems (TCSs), consisting of a histidine sensor kinase (SK) and a cognate response regulator (RR), form the most common mechanism of transmembrane signal transduction in prokaryotes. TCSs in S. coelicolor A3(2) have been analyzed in detail. Here, we identify and classify the SK and RR of S. avermitilis and compare the TCSs with those of S. coelicolor A3(2) by computational approaches. Phylogenetic analysis of the cognate SK-RR pairs of the two species indicated that the cognate SK-RR pairs fall into four classes according to the distribution of their orthologs in other organisms. In addition to the cognate SK-RR pairs, some potential partners of non-cognate SK-RR were found, including those of unpaired SK and orphan RR and the cross-talk between different components in either strain. Our study provides new clues for further exploration of the molecular regulation mechanism of streptomycetes with industrial importance.

关键词： Streptomyces two-component system cross-talk phylogenetic analysis

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Regulation of Starch Biosynthesis in Sweet potato [Ipomoea batatas （L.） Lam.] Towards Starch Quality Improvement

Regulation of Starch Biosynthesis in Sweet potato [Ipomoea b...

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中国植物生理学会第十次会员代表大会暨全国学术年会

作者： Jun Yang and Peng Zhang* SIBS-ETH shanghai Center for Cassava Biotechnology, National laboratory of plant molecular genetics, institute of plant physiology & ecology, shanghai institutes for biological Sciences, {12. academy of Sciences, 300 Fenglin Road, shanghai 2.0032, china

＜正＞Granule-bound starch synthase I （GBSSI）, starch branching enzyme I （SBEI） and starch branching enzyme II （SBEII）, three key enzymes in starch biosynthesis, control starch composition in storage organs of p... 详细信息

关键词： sweet potato starch biosynthesis RNA interference genetic engineering

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Role of oxyR from Sinorhizobium meliloti in Regulating the Expression of Catalases

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Acta Biochimica et Biophysica Sinica 2005年第6期37卷 421-428页

作者： Li EUO~(1.3) Ming-Sheng QI~(1.3) Shi-Yi YAO~2 Hai-Ping CHENG~2 Jia-Bi ZHU~1 Guan-Qiao YU~1 1 National laboratory of plant molecular genetics, institute of plant physiology and ecology, shanghai institutes for biological Sciences.chinese academy of Sciences, shanghai 2.0032. {12. 2 biological Sciences Department, Lehman College, the City University of New York, Bronx, NY 10468. USA, 3 Graduate School of the chinese academy of Sciences, shanghai 2.0032. {12. Nationallaboratoryofplantmoleculargenetics InstituteofPlantPhysiologyandEcologyShanghaiInstitutesforBiologi

The process of symbiotic nitrogen fixation results in the generation of reactive oxygen speciessuch as the superoxide anion（O2-）and hydrogen peroxide（H2O2）.The response of rhizobia to these toxicoxygen species is an important factor in nodulation and nitrogen *** Sinorhizobium meliloti,oneoxyR homologue and three catalase genes,katA,katB,and katC were detected by sequence *** gene is located next to and divergently from katA on the *** investigate the possible rolesof oxyR in regulating the expression of catalases at the transcriptional level in ***,an insertion mutantof this gene was *** mutant was more sensitive and less adaptive to H2O2than the wild typestrain,and total catalase/peroxidase activity was reduced approximately fourfold with the OxyR mutationrelative to *** activities of KatA and KatB and the expression of katA::lacZ and katB::lacZ promoterfusions were increased in the mutant strain compared with the parental strain grown in the absence of H2O2,indicating that katA and katB are repressed by ***,when exposed to H2O2,katA expressionwas also increased in both *** and Escherichia *** exposed to H2O2,OxyR is convertedfrom a reduced to an oxidized form in *** concluded that the reduced form of OxyR functions as arepressor of katA and katB ***,in the presence of H2O2,reduced OxyR is converted to theoxidized form of OxyR that then results in increased katA *** further showed that oxyR expressionis autoregulated via negative feedback.

关键词： Sinorhizobium meliloti OxyR catalase katA H2O2

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Polar auxin transport in rice leaf development

Polar auxin transport in rice leaf development

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2006中国植物细胞发育与分子生物学学术研讨会

作者： Qian Xu, Zhi-Hong Xu, Hong-Wei Xue National key laboratory of plant molecular genetics, institute of plant physiology & ecology, shanghai institutes for biological Sciences, {12. academy of Sciences, 300 Fenglin Road, 2.0032 shanghai, P.R. china.

＜正＞The PAT （polar auxin transport） is required for the leaf pattern formation and vascular differentiation in dicots, however, fewer studies have been performed in monocots. To investigate the effects of PAT on leaf development in monocots, PAT inhibitors were used to treat the rice seedlings. Observations showed that leaves of treated seedlings were twisted irregularly, and in addition, the vascular continuity at the tip and base of leaf blade was defected. The xylogenesis of vascular elements were enhanced, and moter cells became larger, mesophyllous cells and the parenchyma cells around vascular bundle showed massive proliferation.

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水稻eIF3大亚基(eIF3a)编码基因的克隆及其表达模式分析

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实验生物学报 2003年第1期36卷 54-60页

作者：李竑沈思师许智宏薛红卫上海师范大学中国科学院上海生命科学研究院植物生理研究所植物分子遗传国家重点实验室

真核生物翻译起始因子(eIFs)在蛋白合成中起关键作用,在已经鉴定的13个因子中eIF3(由8个或更多的亚基组成)是分子量最大的一个并在翻译起始过程中起着核心作用。eIF3a是eIF3中最大的亚基并介导了eIF3的大多数的功能的实现。利用荧光-差... 详细信息

真核生物翻译起始因子(eIFs)在蛋白合成中起关键作用,在已经鉴定的13个因子中eIF3(由8个或更多的亚基组成)是分子量最大的一个并在翻译起始过程中起着核心作用。eIF3a是eIF3中最大的亚基并介导了eIF3的大多数的功能的实现。利用荧光-差异显示PCR法对生长素处理后的水稻材料进行分析发现eIF3a的表达可被生长素诱导,进一步通过筛选cDNA文库分离出水稻编码eIF3a的全长cDNA(命名为OseIF3a1),OseIF3a1含3459碱基(含5’和3’非翻译区)并编码一个986氨基酸的蛋白,与基因组序列比较表明OseIF3a1基因存在有12.内含子。OSeIF3a1与玉米和烟草的同源蛋白一致性分别为82.4%和70.1%并与其他真核生物eIF3a蛋白具较高一致性。以组织材料进行的RT-PCR结果表明OseIF3a1在根、幼苗、幼穗、茎和叶中表达,启动子-报告基因的转基因结果进一步表明OseIF3a1在根尖及幼嫩叶片表达较高,进一步的RTPCR分析确证了生长素对OseIF3a1的诱导,表明生长素在调控植物生长时可能涉及了翻译水平上的调节。

关键词： eIF3大亚基编码基因水稻克隆表达模式真核翻译起始因子荧光差异显示PCR 生长素

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BP regulates root skewing in gravity-dependent processes

BP regulates root skewing in gravity-dependent processes

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“细胞活动生命活力”——中国细胞生物学学会全体会员代表大会暨第十二次学术大会

作者： Bin Qi,Huiqiong Zheng institute of plant physiology and ecology,shanghai institutes for biological Sciences,chinese academy of Sciences,300 Fenglin Road, shanghai,2.0032.china

plant knotted-like homeobox(KNOX) genes,which encode homeodomain transcription factors and play a key role in maintaining a pluripotent cell population in the shoot apical meristem,have been extensive studied,but their function in root is still *** this study,we show that the BREVIPEDECELLUS(BP) gene,which belongs to KNOXI Class family,is specifically involved in the regulation movements of Arabidopsis *** of bp-1 mutant grown on inclined plate exhibit right-handed axial twisting and the epidermal cells in its elongation zone appeared left-handed *** addition,the root of bp mutant also reduced gravitropic response and displayed a curling phonetype. Further tests show the pattern of auxin-induced gene expression,auxin response,basipetal auxin transport PIN2-green fluorescent protein are altered in bp ***,addition of auxin transport inhibitor TIBA abolishes root-continued skewing of bp mutant or the loss PIN2 function abolishes root curling of bp mutant *** results demonstrate that BP could play an important role in regulating root skewing movement with auxin mediation in gravity-dependent processes.

关键词： Arabidopsis skewing gravitropism KNAT1

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Activation of GPA1, the Heterotrimeric G-protein-Subunit, Suppresses the ftsh-Mediated Inhibition of Chloroplast Development in Arabidopsis

Activation of GPA1, the Heterotrimeric G-protein-Subunit, Su...

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纪念殷宏章先生百年诞辰暨全国光合作用学术研讨会

作者： Lingang Zhang, Qing Wei, Wenjuan Wu, Yuxiang Cheng, Guangzhen Hu, Fenhong Hu, Ying Zhu, Jirong Huang National key laboratory of plant molecular genetics, institute of plant physiology and ecology, shanghai institutes for biological Sciences, chinese academy of Sciences, shanghai {12., china

＜正＞The involvement of the heterotrimeric G-protein signaling pathway in chloroplast development has been demonstrated only by pharmaceutical evidence. In contrast, G-protein knockout mutants have no phenotypic defe... 详细信息

关键词： GPA1 heterotrimeric G-protein-Subunit ftsh-Mediated inhibition Chloroplast development Arabidopsis

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OXP1,a Vulnerable Protein to Oxidative Modification,Plays a Role in Starch Accumulation and Development in Arabidopsis

OXP1,a Vulnerable Protein to Oxidative Modification,Plays a ...

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植物分子生物学与现代农业——2010全国植物生物学研讨会

作者： Xun-Liang Liu~1,Hai-Dong Yu~1,Yuan Guan,Fang-Qing Guo~* The National key laboratory of plant molecular genetics,institute of plant physiology & ecology, shanghai institutes for biological Sciences,{12. academy of Sciences,300 Fenglin Road, shanghai2.0032.china

Carbonylation,an oxidative modification of proteins,is most commonly occurring during abiotic *** is thought to be an irreversible protein damage process and can be used as an overall protein oxidation *** modification usually leads to the loss of function of target proteins,which undergo proteolytic degradation or form

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