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检索条件"作者=~4 state key laboratory of Plant Molecular Genetics,Institute of Plant Physiology and Ecology,Shanghai Institutes for biological {12., Chinese Academy of {12.,Shanghai 2.0032.china"

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Effects of fatty acid synthesis in chloroplast division and embryo development

Effects of fatty acid synthesis in chloroplast division and ...

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中国植物学会植物细胞生物学2010年学术年会

作者： Hong-wei Xue~*,Guo-zhang Wu National key laboratory of plant molecular genetics,institute of plant physiology and ecology,shanghai institutes for biological Science(SIBS),chinese academy of Sciences,300 Fenglin Road,2.0032 shanghai,china

plant lipid metabolism is crucial in cell structure and multiple developmental processes.β-Ketoacyl-[acyl carrier protein]synthase I(KASI) catalyzes the elongation of de novo fatty acid(FA) *** observation of an Arabidopsis T-DNA insertion mutant,kasl,revealed multiple morphological defects including chlorotic leaves,reduced fertility,***,much less chloroplasts of mesophyll cells of chlorotic sectors of young kasl rosette leaves were observed, indicating the suppressed chloroplast division under KASI *** deficiency results in a significant change in the polar lipid composition,which may cause the suppressed expressions of FtsZ and Min system genes and further the disordered Z-ring placement in chloroplast to inhibit the polymerization of FtsZ protein at mid-site of the chloroplast of *** results demonstrate that de novo FA synthesis is crucial for plant growth and polar lipid supply is important for chloroplast division and development.

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Coordinated Replication of Two Internal Origins on Streptomyces Linear Plasmid pFRL1

Coordinated Replication of Two Internal Origins on Streptomy...

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2010年第四届全国微生物遗传学学术研讨会

作者： Ran Zhang and Zhongjun Qin~* key laboratory of Synthetic Biology,shanghai institute of plant physiology and ecology, shanghai institutes for biological Sciences,the chinese academy of Sciences,shanghai,china

Previous reports showed that Streptomyces linear plasmids usually contain one internal replication ***,we identified two new replication loci on pFRL1,one (rep1A/ncs1) next to a telomere and another(rep2A/ncs2)～10 kb from *** rep1A/ncs1 locus was able to direct replication independently in both linear and circular modes,whereas rep2A/ncs2 required an additional locus,rlrA/rorA,in order

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Development of a gene cloning system in fast-growing and moderately-thermophilic Streptomyces and heterologous expression of Streptomyces antibiotics biosynthetic gene clusters

Development of a gene cloning system in fast-growing and mod...

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2010年第四届全国微生物遗传学学术研讨会

作者： Weihua Chen and Zhongjun Qin~* key laboratory of Synthetic Biology,shanghai institute of plant physiology and ecology, shanghai institutes for biological Sciences,the chinese academy of Sciences,shanghai,china

Slow-growing,mesophilic Streptomyces species are a major natural source of antibiotics,whereas fast-growing,thermophilic Streptomyces strains have hardly been *** report here the isolation of 39 fast-growing,moderately thermophilic Streptomyces strains,detection of one linear and three circular plasmids in them,and

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Heterotrimeric G-protein controls chloroplast development via FtsH protease

Heterotrimeric G-protein controls chloroplast development vi...

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2006年拟南芥研究学术研讨会

作者： Qing Wei, Wenjuan Wu, Guangzhen Hu, Fenhong Hu, Jirong Huang National key laboratory of plant molecular genetics, institute of plant physiology and ecology, shanghai institutes for biological Sciences, chinese academy of Sciences, shanghai {12., china

＜正＞Chloroplasts not only endow higher plants with a distinct ability of photosynthesis but also hold many metabolic pathways that are essential for biosynthesis of amino acids, lipids, pigments and hormones, and thus play an important role in crop yield and stress resistance. For sake of a long period of symbiotic evolution, chloroplast development including number, volume and composition is dynamically coordinated with cell sizes and types, and ever changing environmental conditions. Although a marked progress has

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A“Minus and Plus”Method for Cloning Large Genomic Segments in the Linear Plasmid SCP1 of Streptomyces coelicolor A3(2)

A“Minus and Plus”Method for Cloning Large Genomic Segments...

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2010年第四届全国微生物遗传学学术研讨会

The model organism Streptomyces coelicolor A3(2) harbors a 356-kb linear plasmid,*** report here development of a"minus and sequential plus"method for deleting large segment from one telomere of SCP1 followed by seque...

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A novel ASYMMETRIC LEAVES1 and 2 function in Arabidopsis floral organ development

A novel ASYMMETRIC LEAVES1 and 2 function in Arabidopsis flo...

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2006年拟南芥研究学术研讨会

作者： Ben Xu Aiwu Dong Hai Huang National laboratory of plant molecular genetics, shanghai institute of plant physiology and ecology, shanghai institutes for biological Sciences, chinese academy of Sciences, shanghai {12., china state key laboratory of Genetic Engineering, Department of Biochemistry, School of Life Sciences, Fudan University, shanghai 200433, china

＜正＞The Arabidopsis ASYMMETRIC LEAVES1 and 2 （AS1 and AS2） genes are demonstrated to repress class I KNOTTED1-like homeobox genes and to promote the leaf adaxial specifying genes PHABULOSA, PHAVOLUTA and REVOLUTA during leaf morphogenesis. However, very little is known about AS1/2 roles in flower development, although as1 and as2 flowers appear not completely normal. To investigate the AS1/2 function in flower development, we constructed double mutants by combining as1 or as2 with

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Preliminary study of OsNPR1-based gene-engineering for rice disease resistance

Preliminary study of OsNPR1-based gene-engineering for rice ...

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第十三届全国植物基因组学大会

作者： Xiaozun Li,Qun Li,Longjun Zeng,Wenfang Zhao,Zuhua He~* National key laboratory of plant molecular genetics,institute of plant physiology and ecology,shanghai institutes For biological Sciences,chinese academy of Sciences, shanghai 2.0032.china

Systematic acquired resistance(SAR) is regarded as a long-lasting and broad spectrum disease *** studies showed that over-expression of OsNPRI,a master gene for SAR in rice increased resistance to Xanthomonas oryzae pv oryzae(Xoo) and Magnaporthe oryzae,two major rice pathogens. However,the growth and development of OsNPR1 over-expressed(OE) lines are

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A role of At5PTase12.in pollen germination

A role of At5PTase12 in pollen germination

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2006中国植物细胞发育与分子生物学学术研讨会

作者： Yuan Wang, Hong-Wei Xue (National key laboratory of plant molecular genetics, institute of plant physiology & ecology, shanghai institutes for biological Sciences, {12. academy of Sciences, 300 Fenglin Road, 2.0032 shanghai, P.R.china)

＜正＞Inositol polyphosphate 5-phosphatases （5PTases） have been known to be involved in phosphatidylinositol metabolism and affect various aspects of plant growth and development. Presence of multiple members （-15 in arabidopsis） suggests the specific functions of 5PTases in plant cells. Through molecular and genetic approaches, we are trying to study the physiological functions of the At5PTase12. Structural organization analysis of At5PTase12.protein identified a C-terminal localized 5PTase catalytic domain and an N-terminal localized WD-repeat motif that may involve in protein-protein interaction. Semi-quantitative reverse transcription-PCR and promoter-β-glucuronidase reporter gene analysis showed that At5PTase12.gene was mostly expressed in mature pollen grains and relatively less in seedlings and leaves. A T-DNA knock-out mutant of At5PTase12.were further identified and characterized. Analysis on the mutant showed that defected At5PTase12.protein impacted pollen germination in vitro, consistent with the highly expression in mature pollen grains, which was probably correlative with the alteration of cytosolic calcium homeostasis. Preliminary studies suggest that At5PTase12.affects pollen germination through regulating calcium homeostasis in pollen grains.

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Auxin-binding protein in auxin-mediated cell division

Auxin-binding protein in auxin-mediated cell division

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2006年拟南芥研究学术研讨会

作者： Ke Yin, Zhi-Hong Xu, Hong-Wei Xue National key laboratory of plant molecular genetics, institute of plant physiology and ecology, shanghai institutes for biological Science (SiBS), chinese academy of Sciences

＜正＞The plant hormone auxin, playing vital roles in many aspects of plant growth and development, and is essential for various physiological phenomena. However, how auxin transfers its signal and achieve the multiple ends is still puzzled. Recent studies have, on some degree, revealed the functional mechanism on auxin signal transduction at cell surface and relevant gene expressions.

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The Rice 14-3-3 gene family and its involvement in responses to biotic and abiotic stress

The Rice 14-3-3 gene family and its involvement in responses...

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长三角现代植物生物学与农业专题论坛

作者： Fang CHEN, Qun LI, Liangxian SUN, Zuhua HE National key laboratory of plant molecular genetics, institute of plant physiology and ecology, shanghai institutes for biological Sciences, The chinese academy of Sciences, 300 Fenglin Road, shanghai {12., china

＜正＞14-3-3 proteins function as major regulators of primary metabolism and cellular signal transduction in plants. However, their involvement in plant defense and stress responses is largely unknown. In order to better address functions of the rice 14-3-3/GF14 proteins in defense and abiotic stress responses, we examined the rice GF14 family that comprises 8 numbers. The phylogenetic comparison with the Arabidopsis 14-3-3

关键词： Rice 14-3-3 family Biotic and abiotic stress Expression regulation Subcellular localization

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