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Cell Research 2002年第2期12卷 123-132页

作者： MIN ZHANG, BAO HuI ZHANG, LI CHEN, WEI AN1 Institute of Sports Medicine, The Third Hospital, peking university, beijing 100083, china 2Department of Cell Biology, Capital university of medical sciences, beijing 100054, china Institute of Sports Medicine The Third Hospital Peking University Beijing China Department of Cell Biology Capital University of Medical Sciences Beijing China

To investigate whether the expression of exogenous heme oxygenase-1 (HO-1) gene within vascular smooth muscle cells (VSMC) could protect the cells from free radical attack and inhibit cell proliferation, we established an in vitro transfection of human HO-1 gene into rat VSMC mediated by a retroviral vector. The results showed that the profound expression of HO-1 protein as well as HO activity was 1.8- and 2.0-fold increased respectively in the transfected cells compared to the non-transfected ones. The treatment of VSMC with different concentrations of H2O2 led to the remarkable cell damage as indicated by survival rate and LDH leakage. However, the resistance of the HO-1 transfected VSMC against H2O2 was significantly raised. This protective effect was dramatically diminished when the transfected VSMC were pretreated with ZnPP-IX, a specific inhibitor of HO, for 24 h. In addition, we found that the growth potential of the transfected cells was significantly inhibited directly by increased activity of H

关键词： Heme oxygenase retroviral vector free radical cell proliferation vascular smooth muscle cells.

The correlation of multi-angle thermal infrared data and the choice of optimal view angles

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science china Earth sciences 2004年第6期47卷 570-576页

作者： FAN Wenjie1 & XU Xiru1,2 1.Institute of Remote Sensing and GIS of peking university,beijing 100871,china 2.center of Remote Sensing and GIS of beijing Normal university,beijing 100875,china Institute of Remote Sensing and GIS of peking university Beijing China center of Remote Sensing and GIS of beijing Normal university Beijing China

Based on the matrix formula of thermal infrared radiant system, the components temperature can be retrieved by the inversive matrix. Around the stability problem of retrieved result, the research work we did was focused on (i) the correlation of (wk,j) which is the key to affect the retrieval accuracy, (ii) a general method which can help us find the number of retrievable parameters and evaluate the retrieval error before its performance, (iii) the choice of “optimal viewing angle group” based on the formula of absolute error propagation. The row winter wheat field was chosen as an example. The results can provide a theoretical basis for multi-angle thermal infrared remote sensing and components temperature retrieval.

关键词： components temperature retrieval, correlation, number of retrievable parameters, optimal viewing angle group.

Human multi-drug resistant hepatocellular carcinoma induced in nude mice by B-ultrasonographically-directed orthotopic implantation:a new experimental model

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Hepatobiliary & Pancreatic Diseases International 2007年第4期6卷 393-398页

作者： Lei Ding,Xiao-Ping Chen,Zhi-Wei Zhang,Kai Jing and Wan-Guang Zhang Hepatic Surgery center,Affiliated Tongji Hospital,Tongji medical College,Huazhong university of science and Technology,Wuhan 430030,china Department of Oncology center,beijing Shijitan Hospital,beijing 100038,china Hepatic Surgery center Affiliated Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan 430030 China. dinglei1005@***

BACKGROUND:Present studies on the reversal of multi-drug resistance(MDR) are almost entirely limited to in vitro systems,and are seldom carried out in vivo. In order to study MDR under the in vivo situation,a MDR cell strain was used to establish a model in nude mice via orthotopic implantation directed by B-ultrasonography. This model is expected to provide a good platform for evaluating strategies to reverse the phenomenon of primary hepatocellular carcinoma(HCC) MDR. METHODS:An orthotopic MDR1 hepatoma was obtained by injecting the cell lines HepG2 and HepG2/ADM subserosally into the liver of nude mice(10 control and 20 MDR mice). The injections were made under B-ultrasonographic direction. Ultrasonography and laparotomy were used to assess tumor growth,and the long chain PCR technique was applied to sequence the MDR1 gene from multi-drug resistant human HCC cells,HepG2/ADM,and corresponding implanted tumor tissues. Furthermore,MDR1 mRNA and P-gp protein expression were evaluated by reverse transcriptase-polymerase chain reaction(RT-PCR),Western blotting,and immunohistochemical methods. RESULTS:The success rates of tumor implantation and induction of MDR were 100%(30/30) and 95%(19/20),respectively. The 3.8kbp MDR1 gene band was detected from the HepG2/ADM cell line and the correspondingimplanted tumor tissues,and the MDR1 gene sequence coincided with that reported in GenBank. The expressions of MDR1 mRNA and P-gp protein in MDR mice were significantly higher than those in the control group. There was a significant difference in P-gp protein expression between MDR and control mice. CONCLUSION:A MDR model has been successfully established in nude mice via orthotopic implantation of multi-drug resistant human HCC cells directed by ultrasonography.

关键词： nude mice orthotopic implantation carcinoma,hepatocellular drug resistance,multiple

Calcitonin gene-related peptide inhibits interleukin-1β-induced interleukin-8 secretion in human type Ⅱ alveolar epithelial cells

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Acta Pharmacologica Sinica 2006年第10期27卷 1340-1345页

作者： Wen-jing LI Teng-ke WANG Xian WANG~2 Department of Physiology,School of Basic medical sciences,peking university,Key Laboratory of Molecular Cardiovascular science,Ministry of Education,beijing 100083.china Department of Physiology School of Basic Medical Sciences Peking University Key Laboratory of Molecular Cardiovascular Science Ministry of Education Beijing China

Aim:Our previous data have shown that type Ⅱ alveolar epithelial(AEⅡ)cellsexpress neuropeptide calcitonin gene-related peptide(CGRP),and that pro-in-flammatory factor interleukin 1-β(IL-1β)induces CGRP secretion in the A549 hu-man AEⅡ cell *** the present study,we investigated the effect of endogenousand exogenous CGRP on IL-1β-induced chemokine interleukin-8(IL-8)***:We used enzyme-linked immunosorbent assay(ELISA)and RT-PCR todetect IL-8 protein and mRNA levels,respectively,siRNA and the stably trans-fected cell line were used to knock down and overexpress the CGRP gene,respectively,and chemiluminescence assay was used to detect reactive oxygenspecies(ROS)***:CGRP-1 receptor antagonist hCGRP8-37(0.1-1nmol·L-1)greatly amplified IL-1β-induced IL-8 *** inhibition of CGRPexpression by siRNA significantly increased IL-8 secretion upon IL-1β ***,cell clones stably transfected with CGRP showed significantly inhibitedmRNA and protein levels of IL-8 induced by 1L-1β.Conclusion:These data implythat AEⅡ cell-derived CGRP suppress IL-1β-induced IL-8 secretion in an autocrine/paracrine *** investigation showed that CGRP attenuated IL-1β-arousedROS formation,which is an early indication of pro-inflammatory factor signaling.

关键词： type Ⅱ alveolar epithelial cells interleukinl interleukin-8 calcitonin gene-related peptide anti-inflammation lung reactive oxygen species

Status of study on biological and toxicological effects of nanoscale materials

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science china Chemistry 2005年第5期48卷 385-394页

作者： WANG Bing1, FENG Weiyue1, ZHAO Yuliang1, XING Gengmei1, CHAI Zhifang1, WANG Haifang2 & JIA Guang3 1. Laboratory for Bio-Environmental health sciences of Nanoscale Materials (Nanosafety Lab), Institute of High Energy Physics, Chi-nese Academy of sciences, beijing 100049, china 2. College of Chemistry and Molecular Engineering, peking university, beijing 100871, china 3. Department of Occupational and Environmental health sciences, School of Public health, peking university, beijing 100083, china Laboratory for Bio-Environmental health sciences of Nanoscale Materials (Nanosafety Lab) Institute of High Energy Physics Chinese Academy of Sciences Beijing China College of Chemistry and Molecular Engineering Peking University Beijing China Department of Occupational and Environmental health sciences School of Public Health Peking University Beijing China

Because the physical and chemical properties of nanosized materials mostly differ from the existing microsized materials, their potential impacts on human health and the envi-ronment will be topics under the serious discussions in press and in a number of international scientific journals. We analyze and summarize the existing data of the experimental study on the biological activities and adverse effects of nanoscale materials/particles including single wall carbon nanotubes, multi wall carbon nanotubes, titanium oxide and iron powders. Though some biological behaviors of nanoscale materials observed cannot be understood on the basis of the current knowledge, as the existing data are mostly preliminary, it is too early to make some ex-clusive conclusions on biological activities (or the toxicity) of any of nanoscale materials. The experimental techniques, the current topics, and the future research directions for this new re-search field are also discussed.

关键词： nanomaterials, single wall carbon nanotubes, multi wall carbon nanotubes, titanium oxide and iron, biological activity and toxicological effect.

Sampling Survey of Disability in 0-6 Year-old Children in china

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Biomedical and Environmental sciences 2006年第5期19卷 380-384页

作者： HUI ZHANG SHAO-HUA BO XI-TAN ZHANG MIN LIU ZHI-XIANG ZHANG XIAO-LING YANG SHU-RONG JI HUA YAN XIU-LI SUI XIN NA SHAO-HUA GUO ZHENG-LAI WU Institute of Basic medical sciences Chinese Academy of Medical Sciences School of Basic Medicine Peking Union Medical College Beijing 100005 China china Disabled Persons＇ Federation Beijing 100006 China Academy of Military medical sciences Beijing 100071 China School of Public health Health Sciences Center Peking University Beijing 100083 China The First Hospital Peking University Beijing 100034 China Institute for Mental health Peking University Beijing 100083 China china center for Rehabilitation Research Beijing 100068 China General Hospital Tianjin Medical University Tianjin 300052 China The Third Hospital Affiliated to Tanjin medical university Tianjin 300203 China

Objective To investigate the current status of disabled children and prevalence of disabilities in children aged 0-6 years and their risk factors, and to provide scientific evidence for making relevant policies for disabled children. Metlmds In a community-based cross-sectional study, multi-phase, stratified, unequal proportional and cluster sampling was adopted to survey 60 124 children aged 0-6 years. All the investigated children were screened for disabilities, and those with positive screening tests were further diagnosed by various specialties. Results A total of 819 children were diagnosed as disabled with an overall prevalence of 1,362%, 0.155% for hearing disability, 0.160% for visual disability, 0.931% for intelligent disability, 0.424% for limb disability, and 0.101% for mental disability. Prevalence of disability in children was higher in rural areas, and in families with two or more children, low educational level or in divorced families. Conclusion The prevalence of disability can be reduced by economic development, improvement of health care and quality of population, as well as harmonious familial relationship, early prevention of disability, and preschool education for disabled children.

关键词： Children Disability Prevalence Risk factor

Local segment-shared protection based on source egress gateway selection for multi-domain optical mesh networks

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science china(Information sciences) 2011年第2期54卷 227-235页

作者： ZHANG ShengFeng1,3, PENG YunFeng1,2, DU Shu1, YANG XiaoLong1,2 & LONG KePing1,2 1Research center for Optical Internet and Mobile Information Networks, university of Electronic science and Technology of china, Chengdu 611731, china 2Institute of Advanced Network Technology and New Serives (ANTS), university of science and Technology beijing, beijing 100083, china 3Research center for Optical Internet Networks, Chongqing university of Posts and Telecommunications, Chongqing 400065, china Research center for Optical Internet and Mobile Information Networks University of Electronic Science and Technology of China Chengdu China Research center for Optical Internet Networks Chongqing University of Posts and Telecommunications Chongqing China Institute of Advanced Network Technology and New Serives (ANTS) University of Science and Technology Beijing Beijing China

Survivability for multi-domain optical networks is becoming a key parameter due to expanding network. In order to provide the shared-path-protected lightpath in multi-domain optical mesh networks, we propose a protection solution, called GSLSSP. A new source egress gateway selecting mechanism is adopted in this solution. For each connection request, this mechanism can enable the source node to select a better egress gateway node to the destination domain and compute one inter-domain working path near the optimal path with the cooperating of this gateway node, under scalability and domain privacy constraint of multi-domain networks. And then one intra-domain link-disjoint local segment-backup path for each working-segment path is computed out in each single-domain passed by the working path. Simulation evaluation shows that the proposed scheme can provide 100% protection ability for multiple failures. Besides, it is effective in multi-domain path protection with more efficient resource utilization, lower blocking probabilities and less inter-domain cost than other schemes.

关键词： survivability optical mesh networks multi-domains multiple failures segment-shared protection

Recombinant Neural Protein PrP Can Bind with Both Recombinant and Native Apolipoprotein E In Vitro

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Acta Biochimica et Biophysica Sinica 2006年第9期38卷 593-601页

作者： Chen GAO~1 Yan-Jun LEI~(1,2) Jun HAN~1 Qi SHI~1 Lan CHEN~(1,3) Yan GUO~(1,4) Yong-Jun GAO~1 Jian-Ming CHEN~1 Hui-Ying JIANG~1 Wei ZHOU~1 Xiao-Ping DONG~1* 1 State Key Laboratory for Infectious Disease Prevention and Control,National Institute for Viral Disease Control and Prevention,Chinese center for Disease Control and Prevention,beijing 100052,china 2 School of Medicine,Xi'an Jiaotong university,Xi'an 710061,china 3 National Laboratory of medical Molecular Biology,Institute of Basic medical science,Chinese Academy of medical sciences and peking Union medical College,beijing 100005,china 4 College of science and Veterinary Medicine,Northwest Agriculture and Forest university,Yangling 712100,china State Key Laboratory for Infectious Disease Prevention and Control National Institute for Viral Disease Control and Prevention Chinese Center for Disease Control and PreventionBeijing 100052 China School of Medicine Xi'an Jiaotong UniversityXi'an 710061 China National Laboratory of medical Molecular Biology Institute of Basic Medical Science Chinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100005 China College of science and Veterinary Medicine Northwest Agriculture and Forest UniversityYangling 712100 China

The most essential and crucial step during the pathogenesis of transmissible spongiformencephalopathy is the conformational change of cellular prion protein （PrPC） to pathologic isoform (PrPSc).Alot of data revealed that caveolae-like domains （CLDs） in the cell surface were the probable place where theconversion of PrP proteins *** E （ApoE） is an apolipoprotein which is considered toplay an important role in the development of Alzheimer's disease and other neurodegenerative diseases byforming protein complex through binding to the receptor located in the clathrin-coated pits of the cell *** this study,a 914-bp cDNA sequence encoding human ApoE3 was amplified from neuroblastoma cell *** human ApoE isomers were expressed and purified from Escherichia ***-specificantiserum was prepared by immunizing rabbits with the purified ***/His pull-down assay,immunoprecipitation and ELISA revealed that three full-length ApoE isomers interact with the recombinantfull-length PrP protein in *** regions corresponding to protein binding were mapped in the N-terminalsegment of ApoE （amino acid 1-194） and the N-terminal of PrP （amino acid 23-90）.Moreover,the recombinantPrP showed the ability to form a complex with the native ApoE from liver *** data provided directevidence of molecular interaction between ApoE and *** also supplied scientific clues for assessing thesignificance of CLDs on the surface of cellular membrane in the process of conformational conversion fromPrPCto PrPScand probing into the pathogenesis of transmissible spongiform encephalopathy.

关键词： prion disease PrP apolipoprotein E protein interaction caveolae-like domain

Association between ICU quality and in-hospital mortality of V-V ECMO-supported patients—the ECMO quality improvement action(EQIA)study:a national cohort study in china from 2017 to 2019

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Frontiers of Medicine 2024年第2期18卷 315-326页

作者： Wei Cheng Jieqing Chen Xudong Ma Jialu Sun Sifa Gao Ye Wang Longxiang Su Lu Wang Wei Du Huaiwu He Yujie Chen Zunzhu Li Qi Li Jianhua Sun Hongbo Luo Jinbang Liu Guangliang Shan Bing Du Yanhong Guo Dawei Liu Chang Yin Xiang Zhou on behalf of the china National Critical Care Quality Control center-the ECMO quality improvement action(EQIA)study Department of Critical Care Medicine State Key Laboratory of Complex Severe and Rare DiseasesPeking Union Medical College HospitalChinese Academy of Medical Science and Peking Union Medical CollegeBeijing100730China Information center Department/Department of Information Management State Key Laboratory of Complex Severe and Rare DiseasesPeking Union Medical College HospitalPeking Union Medical College&Chinese Academy of Medical SciencesBeijing100730China Department of medical Administration National Health Commission of the People’s Republic of ChinaBeijing100044China School of Population Medicine and Public health Chinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing100730China Department of Epidemiology and Biostatistics Institute of Basic Medicine SciencesChinese Academy of Medical Sciences(CAMS)&School of Basic MedicinePeking Union Medical CollegeBeijing100730China National Institute of Hospital Administration National Health Commission of the People’s Republic of ChinaBeijing100044China 不详

This cohort study was performed to explore the influence of intensive care unit(ICU)quality on in-hospital mortality of veno-venous(V-V)extracorporeal membrane oxygenation(ECMO)-supported patients in *** study involved all V-V ECMO-supported patients in 318 of 1700 tertiary hospitals from 2017 to 2019,using data from the National Clinical Improvement System and china National Critical Care Quality Control *** quality was assessed by quality control indicators and capacity *** the 2563 V-V ECMO-supported patients in 318 hospitals,a significant correlation was found between ECMO-related complications and *** reintubation rate within 48 hours after extubation and the total ICU mortality rate were independent risk factors for higher in-hospital mortality of V-V ECMO-supported patients(cutoff:1.5%and 7.0%;95%confidence interval:1.05–1.48 and 1.04–1.45;odds ratios:1.25 and 1.23;P=0.012 and P=0.015,respectively).Meanwhile,the V-V ECMO center volume was a protective factor(cutoff of≥50 cases within the 3-year study period;95%confidence interval:0.57–0.83,odds ratio:0.69,P=0.0001).The subgroup analysis of 864 patients in 11 high-volume centers further strengthened these ***,ICU quality may play an important role in improving the prognosis of V-V ECMO-supported patients.

关键词： veno-venous extracorporeal membrane oxygenation in-hospital mortality high-volume centers quality control intensive care unit capacity parameters

Antiviral therapy with nucleos(t)ide analogues for severe chronic hepatitis B

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Hepatobiliary & Pancreatic Diseases International 2009年第4期8卷 438-439页

作者： Fu-Kui Zhang, Liver Research center, beijing Friendship Hospital, Capital medical university, beijing 100050,china Capital Med Univ Beijing Friendship Hosp Liver Res Ctr Beijing 100050 Peoples R China

To the Editor:I read the paper by Chen et al[1]with great *** authors performed a retrospective study to evaluate the short-term efficacy of antiviral therapy with

关键词： CHB HBV Antiviral therapy with nucleos t)ide analogues for severe chronic hepatitis B