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2006年拟南芥研究学术研讨会

作者： LI Chun-Hong and CHEN Xiao-Ya National key laboratory of plant molecular genetics, institute of plant physiology and ecology, shanghai institutes for biological sciences, chinese academy of sciences, shanghai 200032, china

＜正＞S-Adenosylhomocystein hydrolase （SAHH）, a key enzyme in S-adenosylmethionine （SAM）-dependent transmethylation reactions, catalyzes the conversion of S-adenosyl-L-homocysteine into adenosine and L-homocystein. Although the biological function of SAHH in human and mice has been elucidated in detail, our understanding of its function and regulation in plants is quite limited.

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Thermostability of photosynthesis in two new chlorophyll b-less rice mutants

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Science china(Life sciences) 2005年第2期48卷 139-147页

作者： LIN Zhifang1,PENG Changlian1,Xu Xinlan1,LIN Guizhu 1 & ZHANG Jingliu2 1.South china institute of Botany,chinese academy of sciences,Guangdong key laboratory of Digital Botanical Garden,Guangzhou 510650,china 2.National key laboratory of plant molecular genetics,institute of plant physiology & ecology,shanghai institutes of biological Sci- ences,chinese academy of sciences,shanghai 200032,china South china institute of Botany Chinese Academy of Sciences Guangdong Key Laboratory of Digital Botanical Garden Guangzhou 510650 China National key laboratory of plant molecular genetics Institute of Plant Physiology & Ecology Shanghai Institutes of Biological Sciences Chinese Academy of Sciences Shanghai 200032 China

Leaves of the two new chlorophyll b-less rice mutants VG28-1, VG30-5 and the wild type rice cv. Zhonghua 11 were subjected to temperatures 28, 36, 40, 44 and 48℃ in the dark for 30 min or gradually elevated temperature from 30℃ to 80℃ at 0.5℃/min. The thermostability of photosynthetic apparatus was estimated by the changes in chlorophyll fluorescence parameters, photosynthetic rate and pigment content, chloroplast ultrastructure and tissue location of H2O2 accumulation. There were different patterns of Fo-temperature curves between the Chl b-less mutants and the wild type plant, and the temperature of Fo rising threshold was shifted 3℃ lower in the Chl b-less mutants (48℃) than in the wild type (51℃). At temperature up to about 45℃, chloroplasts were swollen and thylakoid grana became misty accompanied with the complete loss of photosynthetic oxygen evolution in the two Chl b-less mutants, but chloroplast ultrastruc- ture in the wild type showed no obvious alteration. After 55℃ exposure, the disordered thylakoid and significant H2O2 accumulation in leaves were found in the two Chl b-less mutants, whereas in the wild type plant, less H2O2 was accumulated and the swollen thylakoid still maintained a cer- tain extent of stacking. A large extent of the changes in qP, NPQ and Fv/Fm was consistent with the Pn decreasing rate in the Chl b-less mutants during high temperature treatment as compared with the wild type. The results indicated that the Chl b-less mutants showed a tendency for higher thermosensitivity, and loss of Chl b in LHC II could lead to less thermostability of PSII structure and function. Heat damage to photosynthetic apparatus might be partially attributed to the in- ternal oxidative stress produced at severely high temperature.

关键词： Oryza sativa L, thermostability, chlorophyll b-less mutant, chloroplast ultrastructure, chlorophyll fluorescence, H2O2.

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Activities of floral meristem identity genes in “cauliflower-like” inflorescences of ago1 mutants

Activities of floral meristem identity genes in “cauliflowe...

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中国植物生理学会第十次会员代表大会暨全国学术年会

作者： Han Wang and Yuke He* National key laboratory of plant molecular genetics, institute of plant physiology and ecology, shanghai institutes for biological sciences, chinese academy of sciences, 300 Fenglin Road, shanghai 200032, china

＜正＞Floral development is an important process in the life cycle of plants and floral meristem genes play a key role in the transition to reproductive stage, such as LFY, AP1 and CAL[1,2,3]. MicroRNAs are endogenous... 详细信息

关键词： Arabidopsis Floral meristem genes AGO1 AP1 CAL

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The Effect of THF1 on Kinetics of NPQ Formation and Relaxation of Arabidopsis thalina

The Effect of THF1 on Kinetics of NPQ Formation and Relaxati...

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上海市植物生理学会成立三十周年庆祝活动暨第三届上海市植物生理学青年学术研讨会

作者： Lingang Zhang, Jirong Huang National key laboratory of plant molecular genetics, institute of plant physiology and ecology, shanghai institutes for biological Science, chinese academy of sciences

Nonphotochemical quenching of chlorophyll fluorescence (NPQ) is always regarded as an efficient method to dissipate the excess energy and protect the photosystem

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The differential reversion of ethylene response by ETRl and ERS1 implies functional significance of ethylene receptor collaboration

The differential reversion of ethylene response by ETRl and ...

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2011全国植物生物学研讨会

作者： Qian Liu and Chi-Kuang Wen~* National key laboratory of plant molecular genetics,institute of plant physiology and ecology, shanghai institutes for biological sciences,chinese academy of sciences,300 Fenglin Rd,shanghai 200032,china

In Arabidopsis,the ethylene signal is mediated by a family of five receptor members while little is known about the nature of the receptor signal and how the five receptor members may act *** we show that ETR1,but not ERS 1,was able to reverse ethylene growth inhibitions to a great extent upon 1-MCP,an ethylene antagonist,treatment in the absence of other ***-1,ers 1-1,and ers 1 are ethylene-insensitive ***-1 substantially repressed various ethylene responses independent of other receptor *** inhibition of ethylene response

关键词： Ethylene etr1-1 ers1-1 ers1C65Y receptor collaborations

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Study of Complex Redundancy-Pleiotropy Network of Arabidopsis Ethylene Receptors

Study of Complex Redundancy-Pleiotropy Network of Arabidopsi...

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2010中国青年遗传学家论坛

作者： Qian Liu and Chi-Kuang Wen National key laboratory of plant molecular genetics,institute of plant physiology and ecology,shanghai institutes for biological sciences,chinese academy of sciences,300 Fenglin Rd,shanghai 200032,china

Ethylene responses are modulated by a family of five ethylene receptor members in *** five receptors are genetically redundant while not functionally exchangeable;how the five receptor members may act coordinately remains *** addressed this question by examining the signal output of transmitter-on ETR1 or ERS1 in the absence of other wild-type receptors or in the presence of a wild-type receptor *** to a singling active state by silver or 1-MCP, ETR1 alone was able to reverse ethylene growth inhibition to a great extent while ERS1 was ***-1, ersl-1,and erslC65Y are transmitter-on receptor ***-1 substantially repressed ethylene responses in quintuple receptor knockout mutants while ersl-1 did *** inhibition of ethylene responses by ersl-1 is mainly dependent on ETR1 or ***4 had a greater role than ETR1 in erslC65Y *** lack of both ETR1 and EIN4 abolished the ability of erslC65Y in the repression of ethylene,*** show that the wild-type receptors are not dispensable to the signal output of a transmitter-on ethylene receptor,implying collaborative receptor *** collaborations may facilitate a plant to make corresponding responses to a wide range of ethylene *** redundancy and functional divergence of the five receptor members are essential to the maintenance of collaborative receptor signaling.

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Global Expression Pattern Analysis of Rice RLK Coding Genes in Developing Seeds and in Response to Stress Conditions

Global Expression Pattern Analysis of Rice RLK Coding Genes ...

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植物分子生物学与现代农业——2010全国植物生物学研讨会

作者： Linlin Gao,Hong-Wei Xue~* National key laboratory of plant molecular genetics,institute of plant physiology and ecology, shanghai institutes for biological sciences,chinese academy of sciences,300 Fenglin Road, 200032 shanghai,china

The receptor-like kinase(RLK) plays critical roles in plant growth,development,and stresses responses,through sensing the extracellular signals and activating the downstream signal transduction by phosphorylating the ... 详细信息

关键词： receptor-like kinase rice seed development stress

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Proteomic analysis of rice plasma membrane in early defense response to bacterial blight and research on PM associated proteins

Proteomic analysis of rice plasma membrane in early defense ...

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中国遗传学会七届二次青年研讨会

作者： Fang Chen,Yuexin Yuan,Qun Li,Zuhua He National key laboratory of plant molecular genetics,institute of plant physiology and ecology,shanghai institutes for biological sciences,The chinese academy of sciences,shanghai 200032,china

plant plasma membrane (PM) proteins play important roles in signal perception and transduction during defense response to an attacking pathogen. Through using an improved method of PM protein preparation, and PM-bound GFP fusion protein as a visible marker for routine experiments, we conducted PM proteomics analysis of the rice suspension cells expressing the disease resistance gene Xa21, to identify PM components involved in the early defense response to bacterial blight (Xanthomonas oryzae pv. oryzae). A total of 20 regulated protein spots were observed on 2-D gels of PM fractions at 12h and 24h after pathogen inoculation, of which some were differentially regulated between the incompatible and compatible interactions mediated by Xa21. Eleven protein spots with predicted functions in plant defense were identified by MS/MS, with 9 putative PM-associated proteins including H+-ATPase, protein phosphatase, hypersensitive-induced response protein (OsHIR1), prohibitin (OsPHB2), zinc finger and C2 domain protein, universal stress protein (USP), and LMW heat shock protein. OsHIR1 could be phosphorylated by the microbe challenge, leading to two differentially accumulated protein spots. Finally, gene expression analysis of regulated proteins was performed by RT-PCR, which showed that most, if not all, different proteins’ mRNA level were consistant with their protein level. Besides, some research was also performed on a membrane protein kinase involved in disease resistance. Our study would provide a starting point for the functionality of PM proteins in the rice defense.

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A Proteomics Study of Auxin-Induced Protein Degradation in Arabidopsis thaliana

A Proteomics Study of Auxin-Induced Protein Degradation in A...

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植物分子生物学与现代农业——2010全国植物生物学研讨会

作者： Mei-Qing Xing,Hong-Wei Xue~* National key laboratory of plant molecular genetics,institute of plant physiology and ecology, shanghai institutes for biological sciences,chinese academy of sciences,shanghai 200032 People’s Republic of china

A proteome study based on multi-dimensional non-gel shotgun approach was performed in order to analyze the auxin-induced protein degradation via ubiquitin-proteasome pathway of Arabidopsis *** emphasis was to monitor the overall changes in the protein complexes after auxin *** and 1uM IAA were used to treat 7-day-old seedlings of col-0 and tir1-1 at three time points--6,12,24 hours,more than

关键词： shotgun proteomics auxin protein degradation label-free quantification Arabidopsis

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A Proteasome Inhibitor Protein Mediates Multiple Developmental Processes and Involves in Photomorphogenesis through the Regulation of HY5 Activity in Arabidopsis

A Proteasome Inhibitor Protein Mediates Multiple Development...

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植物分子生物学与现代农业——2010全国植物生物学研讨会

作者： Xin-Xin Han and Hong-Wei Xue~* National key laboratory of plant molecular genetics,institute of plant physiology and ecology, shanghai institutes for biological sciences,chinese academy of sciences,200032 shanghai, People’s Republic of china

The ubiquitin-26S proteasome pathway is a dominant proteolytic system and controls plant growth and development by effectively removing the abnormal proteins and most short-lived regulatory *** regulatory mechanism of 26S proteasome and the function of 26S proteasome inhibitor in plants are still unknown up to now,and we tried to exploit the function ofproteasome inhibitor in Arabidopsis.

关键词： ubiquitin proteasome inhibitor Arabidopsis thaliana HY5 photomorphogenesis

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