检索结果-南通市图书馆

Overcoming multidrug resistance(MDR) in cancer by nanotechnology

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Science china Chemistry 2010年第11期53卷 2226-2232页

作者： BU HuiHui,GAO Yu & LI YaPing Shanghai institute of materia {2.,chinese academy of sciences,Shanghai 2.12.3,china 1. Shanghai institute of materia {2. Chinese Academy of Sciences Shanghai 201203 China

The emerging nanotechnology-based drug delivery holds tremendous potential to deliver chemotherapeutic drugs for treatment of multidrug resistance(MDR) *** drug delivery system could improve the pharmacokinetic behavior of antitumor drugs,deliver chemotherapeutic drugs to target sites,control release of drugs,and reduce the systemic toxicity of drugs in MDR *** review addresses the use of nanotechnology to overcome MDR classified on the bases of the fundamental mechanisms of MDR and various approaches to deliver drugs for treatment of MDR cancer.

关键词： nanotechnology nanoparticles multidrug resistance chemotherapeutic drugs cancer drug delivery system

Assay of mitochondrial functions by resazurin in vitro

在线全文

学校读者我要写书评

暂无评论

Acta Pharmacologica Sinica 2004年第3期25卷 131-135页

作者： Hai-xia ZHANG,Guan-hua DU2.Jun-tian ZHANG institute of materia {2.,Peking Union {2.l College & chinese academy of {2.l sciences,Beijing100050,china institute of materia {2. Peking Union Medical College & Chinese Academy of Medical SciencesBeijing 100050 China

AIM: To study the mechanism of resazurin as indicator of mitochondrial function and to develop a rapid and sensitive assay for measuring metabolic activity of isolated mitochondria from rat liver in vitro. METHODS: The screening was carried out on 96-well microtitre plates by monitoring fluorescence intensity of resazurin reduced by mitochondria. Experimental conditions were optimized and influences of several inhibitors on mitochondrial func- tion were observed. RESULTS: Fluorescence intensity increased in a linear manner when the mitochondrial protein concentration from 5 to 50 μg protein per well was incubated with resazurin (5 μmol/L) during 2.0 min period at 37 oC. Edetic acid could promote the reduction of resazurin in mitochondria. The fluorescence intensity decreased greatly after pretreatment with NaN3, antimycin A, carbonyl cyanide p-trifluoromethoxyphenylhydrazone (FCCP), and oligomycin compared with the control. However, the typical complex I inhibitor, rotenone enhanced the fluorescence intensity without mitochondria. CONCLUSION: Using resazurin to determine mitochondrial func- tion is sensitive, inexpensive and could be easily automated for high throughput screening.

关键词： resazurin drug screening mitochondria

Insulin-sensitizing effects of a novel α-methyl-α-phenoxylpropionate derivative in vitro

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Acta Pharmacologica Sinica 2007年第3期28卷 417-422页

作者： Hao-shu WU~(2.4) Juan-hong YU~(3,4) Ying-yin LI~2.Yu-she YANG~3 Qiao-jun HE~2.Yi-jia LOU~(2.5) Ru-yun JI~3 ~2.epartment of Pharmacology and Toxicology,College of Pharmaceutical sciences,Zhejiang University,Hangzhou 310058,china ~3State Key Laboratory of Drug Research,Shanghai {2. of materia medica,Shanghai {2.s for Biological sciences,Graduate School of the chinese academy of sciences,Shanghai 2.12.3,china Department of Pharmacology and Toxicology College of Pharmaceutical Sciences Zhejiang University Hangzhou 310058 China Department of State Key Laboratory of Drug Research Shanghai Institute of Materia Medica Shanghai Institutes for Biological Sciences Graduate School of the Chinese Academy of Sciences Shanghai 201203 China

Aim:To examine the insulin sensitizing effects of a novel α-methyl-α-phenoxylpropionate derivative YY2. in insulin-sensitive cell ***:Theperoxisome proliferator-activated receptor γ（PPARγ）agonist bioactivities of YY2.were detected by a preadipocyte differentiation ***-PCR and Westernblotting analysis were used to detect the expression of the target gene or *** effects of YY2. on insulin-mediated glucose consumption were determined inthe HepG2.human hepatocellular carcinoma ***:YY2. could enhancethe differentiation of preadipocytes to adipocytes and upregulate the gene ex-pression of PPARγ2.as well as the protein expression of insulin receptor sub-strate-1（IRS-1）,glucose transporter-4（GLUT4）,and adiponectin（ACRP30）.Theeffects on GLUT4 and ACRP30 could be reversed by the PPARγ inhibitor ***,YY2. efficiently reduced glucose consumptions in HepG2.cells after2. h culture,and the effects were related to insulin and YY2. ***:YY2.,a potential insulin-sensitizing agent like rosiglitazone,couldenhance glucose consumption in HepG2.cells in a concentration-and insulin-dependent *** may improve the insulin resistance associated with type 2.iabetes.

关键词： YY2. peroxisome proliferator-activated receptor y insulin sensitivity glucose consumption

STUDIES ON THE CRINUM ALKALOIDS PART I: SYNTHESIS OF ANHYDROLYCORINE-7-ONE

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

chinese Chemical Letters 1996年第1期7卷 13-14页

作者： Hua Wu SHAO Jun Chao CAI (Shanghai institute of materia {2., chinese academy of sciences, Shanghai, 2.0031) Shanghai institute of materia {2. Chinese Academy of Sciences Shanghai 200031

Palladium acetate catalyzed Cyclization of 1-[(6-bromo-1, 3 -benzodioxol- 5-yl)carbonyl 2. 3-dihydro-1H-indole(7a) leading to the anhydrolycorine- 7-one (1a) as the key step inanew Synthesis of Crinum alkaloids is de...

关键词：

Total Synthesis of Przewaquinone A

在线全文

学校读者我要写书评

暂无评论

chinese Chemical Letters 1997年第3期8卷 205-206页

作者： Wen Hu DUAN Jun Chao CAI (Shanghai institute of materia {2., chinese academy of sciences, Shanghai 2.0031) chinese ACAD SCI SHANGHAI INST MAT MEDSHANGHAI 200031PEOPLES R CHINA

Przewaquinone A, a natural orthoquinone was synthesized through several steps, the analytic data of the synthetic target compound was identical with that of przewaquinone A.

关键词： HRMS HNMR Total Synthesis of Przewaquinone A

Centrophenoxine improves chronic cerebral ischemia induced cognitive deficit and neuronal degeneration in rats

在线全文

学校读者我要写书评

暂无评论

Acta Pharmacologica Sinica 2004年第12期25卷 24-30页

作者： Yun LIAO2. Rui WANG, Xi-can TANG3 State KeyLaboratory of Drug Research, Shanghai institute of materia {2., Shanghai institutes for Biological sciences, chinese academyofsciences, Shanghai 2.12.3, china 2.ongji{2.l College of Huazhong Universityof Science & Technology, Wuhan 430030, china Tongji medical College of Huazhong University of Science & Technology Wuhan 430030 China

AIM: To study the effects of centrophenoxine (CPH, meclofenoxate) on chronic cerebral hypoperfusion induced deficits in rats. METHODS: Chronic hypoperfusion in rats was performed by permanent bilateral ligation of the common carotid arteries. Morris water maze was used to measure spatial memory performance. Spectrophoto- metrical techniques were used to assay SOD, GPx activities, MDA content, TXB2. and 6-keto-PGF1α levels. Mor- phological change was examined by HE staining. The expression of Bax and p53 protein were assayed by immuno- histochemistry analysis. RESULTS: Chronic hypoperfusion in rats resulted in spatial memory impairments shown by longer escape latency and shorter time spent in the target quadrant. These behavioral dysfunction were accom- panied by increase in SOD and GPx activities, the content of MDA, the levels of pro-inflammatory mediators (TXB2. 6-keto-PGF1α), overexpression of Bax and P53 protein, and delayed degeneration of neurons in cortex and hippocampus. Oral administration of CPH (100 mg/kg, once per day for 37 d) markedly improved the memory impairment, reduced the increase in antioxidant enzyme activities, MDA content and the levels of pro-inflammatory mediators to their normal levels, and attenuated neuronal damage. CONCLUSION: The abilities of CPH to attenu- ate memory deficits and neuronal damage after ischemia may be beneficial in cerebrovascular type dementia.

关键词： meclofenoxate brain ischemia free radicals maze learning TXB2 6-keto-PGF1α Bax protein p53 protein

Salvicine,a novel topoisomerase Ⅱ inhibitor,exerts its potent anticancer activity by ROS generation

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Acta Pharmacologica Sinica 2007年第9期28卷 1460-1465页

作者： Ling-hua MENG Jian DING Division of Antitumor Pharmacology,State Key laboratory of Drug Research,Shanghai institute of materia {2.,chinese academy of sciences,Shanghai 2.12.3,china Division of Antitumor Pharmacology State Key laboratory of Drug Research Shanghai Institute of Materia Medica Chinese Academy of Sciences Shanghai China

Salvicine is a novel diterpenoid quinone compound obtained by structural modi-fication of a natural product lead isolated from a chinese herb with potent growthinhibitory activity against a wide spectrum of human tumor cells in vitro and inmice bearing human tumor xenografts. Salvicine has also been found to have aprofound cytotoxic effect on multidrug-resisitant (MDR) cells. Moreover, Salvicinesignificantly reduced the lung metastatic foci of MDA-MB-435 orthotopicxenograft. Recent studies demonstrated that salvicine is a novel non-intercalativetopoisomerase II (Topo II) poison by binding to the ATPase domain, promotingDNA-Topo II binding and inhibiting Topo II-mediated DNA relegation and ATPhydrolysis. Further studies have indicated that salcivine-elicited ROS plays acentral role in salvicine-induced cellular response including Topo II inhibition,DNA damage, circumventing MDR and tumor cell adhesion inhibition.

关键词： Salvicine is a novel diterpenoid quinone compound obtained by structural modi-fication of a natural product lead isolated from a chinese herb with potent growthinhibitory activity against a wide spectrum of human tumor cells in vitro and inmice

Synthesis of New 1,6-disubstituted Fulvenes

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

chinese Chemical Letters 1996年第10期7卷 881-883页

作者： Hu XIAO Shu Feng CHEN and Xiao Tian LIANG(institute of materia {2., chinese academy of {2.l sciences, Beijing, 100050) chinese ACAD MED SCI INST MAT MEDBEIJING 100050PEOPLES R CHINA

Sixteen new 1,6-disubstituted fulvenes have been prepared by acylation of cyclopentadienyl sodium and the result of preliminary pharmacological tests showed that several of them have antineoplastic activity.

关键词： Synthesis of New 1 6-disubstituted Fulvenes

Molecular pharmacognosy

在线全文

学校读者我要写书评

暂无评论

Science china(Life sciences) 2010年第6期53卷 643-652页

作者： HUANG LuQi1,XIAO PeiGen2.GUO LanPing1 & GAO WenYuan3 1institute of chinese {2. medica,china academy of chinese medical sciences,Beijing 100700,china 2.0} of Medicinal Plant Development,chinese academy of medical sciences,Peking Union medical College,Beijing 100094,china 3School of Pharmaceutical Science and Technology,Tianjin University,Tianjin 300072.china 1. institute of chinese {2. medica China Academy of Chinese Medical Sciences Beijing 100700 China2. Institute of Medicinal Plant Development Chinese Academy of Medical Sciences Peking Union Medical College Beijing 100094 China3. School of Pharmaceutical Science and Technology Tianjin University Tianjin 300072 China

This article analyzes the background and significance of molecular pharmacognosy,including the molecular identification of medicinal raw materials,phylogenetic evolution of medicinal plants and animals,evaluation and preservation of germplasm resources for medicinal plants and animals,etiology of endangerment and protection of endangered medicinal plants and animals,biosynthesis and bioregulation of active components in medicinal plants,and characteristics and the molecular bases of top-geoherbs.

关键词： molecular pharmacognosy pharmacognosy borderline subject